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Antigen unmasking does not improve the visualization of phospholipase C zeta in human spermatozoa

Asian Journal of Andrology 2022年第4期24卷 345-352页

作者： Xin Meng Celine Jones Pedro Melo caroline ross Ginny Mounce Tim Child Kevin Coward Nuffield Department of Women's and Reproductive Health University of OxfordOxford OX39DUUK The Women's Centre Oxford University Hospitals NHS Foundation TrustOxford OX39DUUK xford Fertility Oxford Business Park NorthOxford OX42HWUK.

Phospholipase C zeta(PLCζ)is a sperm-specific protein that triggers oocyte *** analysis of PLCζexpression in human spermatozoa can be used as a diagnostic marker for oocyte activation *** laboratory has previously optimized a standard"in-house"assay to determine PLCζexpression in human ***,one study has suggested that an antigen unmasking method(AUM)would be more efficient in visualizing PLCζin human *** study aimed to compare our established assay and AUM(involving HCl,acidic Tyrode's solution[AT],and heat).The mean relative fluorescence(RF)intensity of PLCζin frozen-thawed spermatozoa from fourteen fertile donors stained with the in-house method was significantly higher than three other AUM groups(in-house[mean±standard error of mean]:18.87±2.39 arbitrary units[a.u.]vs non-AUM:11.44±1.61 a.u.,AT-AUM:12.38±1.89 a.u.,and HCl-AUM:12.51±2.16 a.u.,P<0.05,one-way analysis of variance).The mean RF intensity of PLCζin AT-and HCl-treated spermatozoa from 12 infertile males was not significantly different from that of the non-AUM ***,the in-house method resulted in the highest RF intensity(12.11±1.36 a.u.,P<0.01).Furthermore,specificity testing of antibody-antigen binding indicated that the in-house method showed more specific binding than spermatozoa treated by the *** conclusion,our in-house method showed superior visualization and reliability than the AUM,thus supporting the continued use of our in-house assay for clinical research screening.

关键词： antigen unmasking immunofluorescence staining male infertility phospholipase C zeta

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Parallel personal measurements of diesel engine exhaust and crystalline silica using dual sampling port

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International Journal of Mining Science and Technology 2020年第3期30卷 313-319页

作者： Alan Fleck caroline Couture Virginie Cabelguen Patrick Eddy Ryan ross Thuot Guillaume Lachapelle Hugo Coulombe Maximilien Debia Department of Environmental and Occupational Health University of MontrealMontreal H3T 1A8Canada Health and Safety Agnico Eagle MinesToronto M5C 2Y7Canada Health and Safety Westwood Mine(IAMGOLD)Preissac J0Y 2E0Canada

Diesel engine exhaust(DEE) and crystalline silica exposures occur simultaneously in the mining industry,and occupational sampling campaigns can be time-and cost-consuming. The authors evaluated a dualport system for simultaneous sampling of DEE and crystalline silica in laboratory and field *** tests evaluated the operation of pumps during 8 h sampling and the intensity of the flow variation for various filter loading conditions and for different modes of operation. Field validation was performed in an underground mine. Pumps operated in constant flow or constant pressure *** in constant flow mode showed that when the flowrate increased on one side of the system, it decreased on the opposite side according to the loading intensity. Tests in constant pressure mode showed that flowrates systematically decreased when using loaded cassettes. However, the higher the backpressure setting, the lower the flow variation was. Flow variations during field tests were generally within the acceptable ±5% range. However, significant flow variations were identified in higher concentrations. A significant negative correlation was found between flowrate variation and total carbon concentration. While the majority of tests support the use of the dual-port for evaluating concomitant exposures, results highlight the possibility of filter overloading as a cause of flowrate changes.

关键词： Crystalline silica Diesel engine exhaust Dual-port Mining Monitoring

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基于核磁共振的代谢组学方法研究绵羊围孕期营养过剩对于血浆代谢物的影响

基于核磁共振的代谢组学方法研究绵羊围孕期营养过剩对于血浆代谢...

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第十九届全国波谱学学术会议

作者：李娟 Horst Joachim Schirra Song Zhang Janna Morrison caroline McMillen ross L.Tellam 华东师范大学物理与材料学院上海市磁共振重点实验室澳大利亚CSIRO动物食物与健康科学研究所澳大利亚昆士兰大学高级影像学中心澳大利亚南澳大学药学与医学学院

＜正＞肥胖症患者的数量在全球范围内的急剧上升对现代社会的人类健康造成了极大的威胁,这是因为肥胖是许多慢性疾病的主要危险因素,包括高血压,心血管疾病,癌症和Ⅱ型糖尿病等等。有越来越多的证据表明母体怀孕期间的肥胖或营养过剩等...

＜正＞肥胖症患者的数量在全球范围内的急剧上升对现代社会的人类健康造成了极大的威胁,这是因为肥胖是许多慢性疾病的主要危险因素,包括高血压,心血管疾病,癌症和Ⅱ型糖尿病等等。有越来越多的证据表明母体怀孕期间的肥胖或营养过剩等症状对于后代的能量代谢有着长期的影响。目前已发现婴儿出生时的体重以及儿童期肥胖和青春期肥胖均与母体肥胖有直接的联系,但是与之相关的生理机制尚不清楚。代谢组学作为一门新兴学科已

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Better Than You Think—Appropriate Use of Implantable Cardioverter-Defi brillators at a Single Academic Center:A Retrospective Review

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Cardiovascular Innovations and Applications 2021年第2期5卷 235-243页

作者： Nikhil H.Shah Steven J.ross Steve A.Noutong Njapo Justin Merritt Andrew Kolarich Michael Kaufmann William M.Miles David E.Winchester Thomas A.Burkart Matthew McKillop UF Division of Cardiovascular Medicine 1600 SW Archer RdPO Box 100277GainesvilleFL 32610USA UVA Division of Cardiovascular Medicine PO Box *** Lee St.CharlottesvilleVA 22908-0158USA The Johns Hopkins Hospital Department of Radiology 601 N Caroline StBaltimore 21287USA The Heart Center 930 Franklin Street SEHuntsvilleAL358015USA Intermountain Medical Center 1380 E Medical Center DrSte 1500St.GeorgeUT 847906USA Carolina Cardiology Consultants Prisma Health1005 Grove RoadGreenvilleSC 29605USA

Background:Implantable cardioverter-defi brillators(ICDs)can be life-saving devices,although they are expensive and may cause *** 2013,several professional societies published joint appropriate use criteria(AUC)assessing indications for ICD *** evaluating the clinical application of AUC are *** registry-based studies estimated that 22.5%of primary prevention ICD implantations were“non-evidence-based”*** the basis of AUC,we aimed to determine the prevalence of“rarely appropriate”ICD implantation at our institution for comparison with previous ***:We reviewed 286 patients who underwent ICD implantation between 2013 and *** of each ICD implantation was assessed by independent review and rated on the basis of ***:Of 286 ICD implantations,two independent reviewers found that 89.5%and 89.2%,respectively,were appropriate,5.6%and 7.3%may be appropriate,and 1.8%and 2.1%were rarely *** AUC indication was found for 3.5%and 3.4%of ICD implantations,*** prevention ICD implantations were more likely rarely appropriate(2.6%vs.1.2%and 3.6%vs.1.1%)or unrated(6.0%vs.1.2%and 2.7%vs.0.6%).The reviewers found 3.5%and 3.4%of ICD implantations,respectively,were non-evidence-based *** difference in rates between reviewers was not statistically signifi ***:Compared with prior reports,our prevalence of rarely appropriate ICD implantation was very *** high appropriate use rate could be explained by the fact that AUC are based on current clinical *** AUC could benefi t from additional secondary prevention *** importantly,clinical judgement and individualized care should determine which patients receive ICDs irrespective of guidelines or criteria.

关键词： Appropriate use appropriate use criteria implantable cardioverter-defi brillator

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