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The Use of Erythropoietin in Liver Regeneration in Pigs after Partial Hepatectomy

Surgical Science 2015年第11期6卷 518-526页

作者： Henrique de Aguiar Wiederkehr caroline aragao de carvalho Julio Cesar Wiederkehr Ricardo Ribeiro Gama Eros Luiz de Sousa Teresa Cristina Cavalcanti Taíse Fuchs Barbara de Aguiar Wiederkehr Lucas Mansano Sarquis Faculdade Evangelica do Paraná CuritibaParanáBrazil department of Surgery Federal University of ParanáCuritibaParanáBrazil department of Experimental Surgery Faculdade Evangélica do ParanáCuritibaParanáBrazil department of Veterinary Medicine Faculdade Evangélica do ParanáCuritibaParanáBrazil department of Pathology Federal University of ParanáCuritibaParanáBrazil department of Veterinary Anesthesiology Faculdade Evangélica do ParanáCuritibaParanáBrazil

A partial hepatectomy is a surgical procedure performed during the living-donor liver transplantation and sometimes the only option for patients with hepatocarcinoma. However the remnant liver after the hepatectomy is still a major concern. Therefore, the process of liver regeneration has been a constant theme of study in order to optimize this process. Erythropoietin, a hormone produced by the kidney and involved in protecting organs like heart, liver and kidney itself against injuries can be one of these factors that could accelerate the liver regeneration. This study aims to observe if erythropoietin can accelerate the process of liver regeneration after partial hepatectomy in pigs. Methods: 8 pigs were classified into 2 groups of 4 pigs each: the control group and the test group. The animals in the first group underwent an application of saline solution subcutaneous on the day before the hepatectomy. Instead of saline solution, the test groups received a subcutaneous injection of 200 UI/lg of recombinant erythropoietin also on the day before the surgical procedure. After 7 days since the hepatectomy, in each animal the liver was biopsied in two regions, one next to the hepatectomy section and other far from it. The liver regeneration was analyzed using Ki-67. Results: Pigs from control group presented the following results: Control pig I: 30% of regeneration in the hepatectomy section and 10% in the region far from it;control pig II 24% and 4%;control pig III 27% and 7%. The test group presented no significant liver regeneration since Ki-67 could not identify cell proliferation in neither the biopsied areas. Conclusion: Since the number of pigs was not statistically significant, we could not conclude any further hypothesis. We strong believe that enhancing the number of pigs and testing different doses, we will be able to reach further conclusions.

关键词： Liver Regeneration Partial Hepatectomy Erythropoietin Pigs

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Turning Industrial Waste into a Valuable Bioproduct:Starch from Mango Kernel derivative to Oil Industry Mango Starch derivative in Oil Industry

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Journal of Renewable Materials 2019年第2期7卷 139-152页

作者： Nívia do Nascimento Marques caroline Suzy do Nascimento Garcia Liszt Yeltsin Coutinho Madruga Marcos Antônio Villetti Men de SáMoreira de Souza Filho Edson Noriyuki Ito Rosangela de carvalho Balaban Instituto de Química Universidade Federal do Rio Grande do Norte-UFRNNatal59078970Brazil departamento de Física Universidade Federal de Santa Maria-UFSMSanta Maria97105900Brazil Embrapa Agroindústria Tropical Fortaleza60511-110Brazil departamento de Engenharia de Materiais Universidade Federal do Rio Grande do Norte-UFRNNatal59078970Brazil

After industrial mango processing,tons of residues such as peels and kernels are discarded as ***,almost 60% of the mango kernel is due to starch on a dry weight ***,starch from mango(Manguifera Indica L.)kernel was applied to obtain a starch fatty ester with vinyl laurate,in DMSO,under basic ***,1H and 13C NMR confirmed that a starch ester with a degree of modification of 2.6 was *** showed that the modified starch has higher thermal stability than its precursors and higher than a vinyl laurate/starch physical *** data showed that granular shape and smooth surface on mango starch changed after chemical modification to a continuous and shapeless *** industrial reject derivative behaved as an efficient alternative environmentally friendly fluid loss controller in oil drilling fluids,even in conditions of high temperatures and high pressures(HTHP)drilling.

关键词： Mango Starch oil drilling fluid transesterification vinyl laurate

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α,β-amyrin, a natural triterpenoid ameliorates L-arginine-induced acute pancreatitis in rats

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World Journal of Gastroenterology 2010年第34期16卷 4272-4280页

作者： caroline Mouro Melo Karine Maria Martins Bezerra carvalho Julliana Catharina de Sousa Neves Talita Cavalcante Morais Vietla Satyanarayana Rao Flávia Almeida Santos Gerly Anne de Castro Brito Mariana Helena Chaves department of Physiology and Pharmacology Biomedical Institute of Brazilian Semiarid Faculty of Medicine Federal University of Ceará department of Morphology Faculty of Medicine Federal University of Ceará department of Organic and Inorganic Chemistry Federal University of Piauí

AIM: To study the benef icial effects of triterpene α,β-amyrin and the underlying mechanisms in an experimental pancreatitis model. METHODS: Acute pancreatitis was induced in five groups of rats (n = 8) by L-arginine (2 × 2.5 g/kg, intraperitoneal, 1 h apart) and 1 h later, they received a single oral dose of α,β-amyrin (10, 30 and 100 mg/kg),methylprednisolone (30 mg/kg) and vehicle (3% Tween 80). A saline (0.9% NaCl) treated group served as a normal control. Efficacy was assessed at 24 h by determination of serum levels of amylase, lipase and proinflammatory cytokines [tumor necrosis factor (TNF)-α and interleukin (IL)-6], pancreatic myeloperoxidase (MPO) activity, lipid peroxidation [thiobarbituric acid reactive substances (TBARS)], nitrate/nitrite levels, and the wet weight/body weight ratio. Tissue histology and the immunoreactivity for TNF-α and inducible nitric oxide synthetase (iNOS) were performed. RESULTS: α,β-amyrin and methylprednisolone treatments significantly (P decreased the serum levels of amylase and lipase, and TNF-α and IL-6, as compared to the vehicle control. Also, pancreatic levels of MPO activity, TBARS, and nitrate/nitrite were signifi cantly lower. Histological f indings and TNF-α and iNOS immunostaining further confirmed the amelioration of pancreatic injury by α,β-amyrin. CONCLUSION: α,β-amyrin has the potential to combat acute pancreatitis by acting as an anti-in? ammatory and antioxidant agent.

关键词： Acute pancreatitis L-arginine Cytokines Lipid peroxidation α,β-amyrin

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Noninvasive diagnosis of periportal fibrosis in schistosomiasis mansoni:A comprehensive review

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World Journal of Hepatology 2022年第4期14卷 696-707页

作者： Joelma carvalho Santos caroline Louise Diniz Pereira Ana Lúcia Coutinho Domingues Edmundo Pessoa Lopes Postgraduate Program in Tropical Medicine Center of Health SciencesUniversidade Federal de PernambucoRecife 50670-901PernambucoBrazil Gastroenterology Division Department of Internal Medicine of Center of Health SciencesHospital das Clínicas-Universidade Federal de PernambucoRecife 50670-901PernambucoBrazil

Schistosomiasis mansoni is a neglected disease and key public health problem,mainly due to its high prevalence,the scarcity of public policies,and the severity of some clinical *** fibrosis(PPF)is the commonest complication of chronic schistosomiasis mansoni and its diagnosis requires different *** though wedge biopsy of the liver is considered the gold standard,it is not justified in non-surgical patients,and percutaneous liver biopsy may be informative but does not have sufficient *** PPF tests mostly include biological(serum biomarkers or combined scores)or physical assessments(imaging assessment of fibrosis pattern or tissue stiffness).Moreover,imaging techniques,such as ultrasound,computed tomography,magnetic resonance imaging,and elastography are applied not only to support the diagnosis of schistosomiasis,but also to assess and detect signs of portal hypertension and organ damage due to chronic schistosomiasis.A combination between a comprehensive history and physical examination with biomarkers for liver fibrosis and imaging methods seems to offer the best approach for evaluating these *** addition,understanding their strengths and limitations will allow a more accurate interpretation in the clinical context and can lead to greater accuracy in estimating the degree of fibrosis in patients with Schistosomiasis mansoni(***)*** review will discuss the different noninvasive methods that are currently available for the evaluation of PPF in *** infection,and their application,advantages,and limitations in clinical practice.

关键词： Schistosoma mansoni Parasitic liver diseases Liver fibrosis Biomarkers Elasticity imaging techniques Ultrasonography

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Liver Transplantation in a Patient with Situs inversus Associated with Biliary Atresia, Polysplenia and Absence of Inferior Vena Cava: A Case Report

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Surgical Science 2016年第2期7卷 89-95页

作者： Henrique de Aguiar Wiederkehr caroline Aragão de carvalho Matheus Ruggeri Lucca Cajazeira Campos Barbara de Aguiar Wiederkehr Julio Cesar Wiederkehr School of Medicine Faculdade Evangélica do Paraná (FEPAR) Curitiba Brazil department of Surgery Federal University (UFPR) Curitiba Brazil

Biliary atresia (BA) is the result of a process of unknown etiology which can occur as an isolated lesion or in association with several congenital abnormalities and remains the main indications of liver transplantation among infants. One of the malformations associated with biliary atresia is Situs inversus totalis, which is a rare congenital abnormality found in 10% - 20% of infants with biliary atresia and presents an incidence varying from 1:5000 to 1:20,000. This case report aims to present an extremely rare case of a patient with Situs inversus associated with biliary atresia, polysplenia and abscence of inferior vena cava that underwent liver transplantation. Case Report: A 10-month-old boy was referred to our hospital with the diagnosis of cirrhosis, due to biliary atresia. Abdominal ultrasound and Doppler scan showed the liver in the left upper quadrant as well as signs of chronic liver disease. In addition, we could notice that the spleen had several lobes, suggesting polysplenia, and was positioned in the right upper quadrant. The patient underwent living donor liver transplant. His mother was the donor and the graft, segment III, was positioned in the left upper quadrant associated with a Y en Roux reconstruction of the biliary tract. During the first post-operatory day, the patient presented signs of disseminated sepsis. Laboratory and imaging diagnostic tests were performed presenting a small intestinal malrotation associated with diffuse ischemia. The patient died at the third day of PO due to complications related to this condition. Conclusion: Any patient with Situs inversus with acute abdomen should be aggressively investigated for other structural and biochemical abnormalities. If liver transplant is indicated, the procedure can be successfully performed.

关键词： Liver Transplantation Situs inversus Polysplenia Biliary Atresia

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Biomechanical Analysis of Implant Assisted-Overdentures with Variations in the Attachments Systems

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Materials Sciences and Applications 2015年第8期6卷 734-742页

作者： Eduardo Vedovatto Jose Vitor Quinelli Mazaro Humberto Gennari Filho Paulo Sérgio Perri de carvalho caroline Cantieri de Mello Cleidiel Aparecido Araujo Lemos S&atilde o Leopoldo MandicInstituteandResearch Center Campinas Brazil department of Prosthodontic and dental Materials Ara&ccedil atuba School of dentistry S&atilde o Paulo State University (UNESP) Ara&ccedil atuba Brazil department of Surgery an Integrated Clinic Ara&ccedil atuba School of dentistry S&atilde o Paulo State University (UNESP) Ara&ccedil atuba Brazil departmentof dental Materials and Prosthodontics Ara&ccedil atuba Schoolofdentistry S&atilde o Paulo StateUniversity (UNESP) Ara&ccedil atuba Brazil.

The aim of this study was to assess the stress patterns on the peri-implant zone and residual alveolar ridge in different overdenture attachment system designs by the photoelasticity method. Four attachments systems were tested: O-ring, ERA, Bar-clip, and Bar-clip/O-ring association. The prostheses were loaded with 100 N in five pre-determined points and the photoelastic model was evaluated by a circular polariscope. The anterior load, O-ring, and ERA showed better stress distribution in relation to the bars systems, which presented stress levels surrounding implants. The molar load, Bar-clip/O-ring association, presented the biggest stress concentration on the peri-implant region in relation to the others. When the second molar was loaded, there was a concentration of stress in the alveolar ridge in all situations analyzed. Within the limitation of this “in vitro” study, it could be concluded that there were biomechanical differences among the attachments systems analyzed, principally between isolated and the bars systems. The O-ring showed better stress distribution and the Bar-clip/O-ring showed higher stress concentration.

关键词： dental Implants Complete denture Overdentures

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Genotyping and Antifungal Susceptibility Profile of Sequential Candida albicans Isolated from the Oral Cavity of HIV-Infected Individuals

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Journal of Pharmacy and Pharmacology 2018年第4期6卷 350-361页

作者： Daniela Vanessa Moris Joyce Marinho de Souza Marcia Souza carvalho Melhem Marilena dos Anjos Martins caroline Lucio Moreira Lenice Rosario de Souza Gioconda San-Blas Maria Walderez Szeszs Lidia Raquel carvalho Marcus Vinicius Pimenta-Rodrigues Rinaldo Poncio Mendes Tropical Diseases Area--Faculdade de Medicina de Botucatu Universidade Estadual Paulista "Julio de Mesquita Filho "--UNESP Sao Paulo Botucatu 18600-000 Brazil Masters in Health Seience--Universidade do Oeste Paulista--UNOESTE Sao Paulo Presidente Prudente 19067-175 Brazil department of Biomedical Sciences--Universidade do Oeste Paulista--UNOESTE Presidente Prudente 19050-920 Brazil Instituto Adolfo Lutz Sao Paulo 01246-000 Brazil Venezuelan Institute for Scientific Research Center for Microbiology and Cell Biology Caracas 11 01204 Venezuela Biostatistics department Instituto de Biociencias Universidade Estadual Paulista "Julio de Mesquita Filho "--UNESP Sao Paulo Botueatu 18600-000 Brazil

Objective： The study aimed to evaluate the genotypic profiles of C. albicans （Candida albicans） sequentially isolated throughout the course of HIV infections, and to determine its MIC （minimal inhibitory concentrations） to AMB （amphotericin B）, FLC （fluconazole）, KTC （ketoconazole）, and ITC （itraconazole）. design： samples were collected from the oral cavity of HIV-positive individuals during 4 years, with a sterilized swab. MIC was performed by using the microdilution method AFST/EUCAST. The genetic similarities within and between sequential clones of C. albicans were assessed by DNA fingerprinting using the random amplification ofpolymorphic DNA technique. Results： A total of 142 oral samples were isolated from 59 HIV-infected individuals who attempted up to five visits each, with or without symptoms of oropharyngeal candidiasis. Profile analysis revealed that yeasts isolated over sequential visits from symptomatic or asymptomatic individuals showed 78% or 87% relatedness, respectively. The degree of similarity among C. albicans was higher for isolates from colonization than for those from infection. Genetically identical C. albicans samples also formed connected subelusters in sequential visits. In regard to susceptibility profile, all isolates were susceptible to AMB, FLC, KTC, and ITC and maintained this pattern all along, no differences in MICs of any given antifungal compound were observed for sequential C. albicans isolates. Conclusions： These data suggest that genotype and susceptibility to antifimgal drugs were maintained over time in sequentially isolates of C. albicans colonization and a diverse evolutionary genetic trend in C. albicans sequentially isolated from the oral eandidiasis of HIV infected individuals.

关键词： Genotyping Candida albicans sequential HIV- positive individuals oral cavity.

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Disentangling spatial,environmental and historical effects on tropical forest tree species turnover

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Journal of Plant Ecology 2021年第4期14卷 717-729页

作者： Alisson Borges Miranda Santos Vinicius Andrade Maia Cleber Rodrigo de Souza Nathalle Cristine Alencar Fagundes Fernanda Moreira Gianasi Aurelio de Jesus Rodrigues Pais Natalia de Aguiar-Campos Gabriela Gomes Pires Diego Teixeira Girardelli Jean Daniel Morel Mariana caroline Moreira Morelli Felipe de carvalho Araujo Rubens Manoel dos Santos department of Forestry Sciences Federal University of LavrasUniversity CampusLavrasBrazil Biological Sciences University of the State of Minas GeraisItuiutaba UnitItuiutabaBrazil department of Biology Federal University of LavrasUniversity CampusLavrasBrazil Faculty of Environmental Engineering and Natural Resources Zambeze UniversityChimoioMozambique

Aims We aimed at disentangling the effects of spatial distance,current and past environmental dissimilarity,and their combinations on tree community taxonomic and phylogenetic turnover by addressing the following questions:(i)Is tree community taxonomic and phylogenetic turnover related to the indirect effects of spatial distance via environmental dissimilarity?(ii)Does tree community taxonomic and phylogenetic turnover respond to paleoclimate(Last Glacial Maximum and Mid-Holocene)?Methods The study was carried out in 14 Atlantic rainforest sites in Brazil(20.4 ha sampled)containing 615 tree species from 83 plant *** obtained plot-level geographic coordinates and soil variables and site-level bioclimatic variables in the current,Mid-Holocene and Last Glacial *** used structural equation models with a distance-based approach to(i)test the direct effects of spatial distance and environmental dissimilarity and(ii)test the indirect effects of spatial distance via environmental dissimilarity on taxonomic(Bray–Curtis distance)and phylogenetic turnover(Comdist and Comdistnt distances).Important Findings Our results suggest a weak indirect effect of spatial distance via environmental dissimilarity on taxonomic and phylogenetic *** community turnover was driven by the direct effects of neutral,niche-based and historical ***,we inferred that the paleoclimate(historical processes)promoted the selection of the clades that gave rise to the current flora,while spatial distances(neutral processes)limited the dispersal range of species from the regional pool and environmental conditions(niche-based processes)locally selected the taxa that are able to persist.

关键词： assembly processes phylogenetic communities environmental filtering neutral communities phylogenetic turnover ecological niche paleoclimate effects

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Preservation of platelet function in patients with cirrhosis and thrombocytopenia undergoing esophageal variceal ligation

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Hepatobiliary & Pancreatic Diseases International 2020年第6期19卷 555-560页

作者： Evandro de Oliveira Souza Elbio Antonio D’Amico Tania Rubia Flores da Rocha caroline Marcondes Ferreira Juliana Medeiros Batista Luiz Augusto CarneiroD’Albuquerque Flair Jose Carrilho Alberto Queiroz Farias Division of Gastroenterology and Hepatology University of Sao Paulo School of MedicineAv.Dr.Eneas Carvalho de Aguiar2559th flooroffice 9159Sao PauloSP 05403-000Brazil Hemostasis Laboratory Hematology ServiceUniversity of Sao Paulo School of MedicineAv.Dr.Eneas Carvalho de Aguiar2552nd floorSao PauloSP 05403-000Brazil

Background:Thrombocytopenia is a possible risk factor for bleeding after band ligation of esophageal ***,elevated von Willebrand factor(VWF)in cirrhosis improves platelet function and could decrease this *** objective was to assess platelet function in patients with cirrhosis undergoing esophageal variceal ligation(EVL).Methods:The assessment consisted of platelet count,antigen and activity of VWF and VWF-cleaving protease ADAMTS-13 activity,and a platelet adhesion and aggregation test simulating vascular flow in vivo(Impact-RR)prior to ***:Totally 111 patients were divided into three groups according to platelet count:(1)100×109/L(n=26,23.4%).No statistically significant difference was found in the aggregate size of platelets[group 1:41.0(31.8–67.3)μm 2;group 2:47.0(33.8–71.3)μm 2;and group 3:47.0(34.0–66.0)μm 2;P=0.60]and no significant correlation was found between aggregate size and platelet count(Spearman r=0.07;P=0.47).Surface coverage was 4.1%(2.8%–6.7%),8.5%(4.0%–10.0%),and 9.0%(7.1%–12.0%)(Pdemonstrated for platelet function ***:Platelet function is preserved even in the presence of thrombocytopenia,including in the patients with post-EVL bleeding.

关键词： Cirrhosis Hemostasis Thrombocytopenia Platelet aggregation Endoscopy Hemorrage

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Neurorestorative Effects of Constraint-Induced Movement Therapy after Stroke: An Integrative Review

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Neuroscience & Medicine 2013年第4期4卷 253-262页

作者： Marina Lucas Pedro Ribeiro Mauricio Cagy Silmar Teixeira Fernanda Chaves Diana carvalho caroline Peressutti Sérgio Machado Juliana Bittencourt Bruna Velasques Roberto Piedade Brain Mapping and Sensory Motor Integration Institute of Psychiatry of Federal University of Rio de Janeiro (IPUB/UFRJ) Rio de Janeiro Brazil Brain Mapping and Sensory Motor Integration Institute of Psychiatry of Federal University of Rio de Janeiro (IPUB/UFRJ) Rio de Janeiro Brazil Physiotherapy Laboratory Veiga de Almeida University (UVA) Rio de Janeiro Brazil Physiotherapy Department Piquet Carneiro Policlinic State University of Rio de Janeiro (UERJ) Rio de Janeiro Brazil Brain Mapping and Sensory Motor Integration Institute of Psychiatry of Federal University of Rio de Janeiro (IPUB/UFRJ) Rio de Janeiro Brazil School of Physical Education Bioscience Department Federal University of Rio de Janeiro (EEFD/UFRJ) Rio de Janeiro Brazil Institute of Applied Neuroscience (INA) Rio de Janeiro Brazil Brain Mapping and Sensory Motor Integration Institute of Psychiatry of Federal University of Rio de Janeiro (IPUB/UFRJ) Rio de Janeiro Brazil Institute of Applied Neuroscience (INA) Rio de Janeiro Brazil Division of Epidemiology and Biostatistic Institute of Health Community Federal Fluminense University (UFF) Rio de Janeiro Brazil Laboratory of Panic and Respiration Federal University of Rio de Janeiro (UFRJ) Rio de Janeiro Brazil Physical Activity Neuroscience Laboratory Salgado de Oliveira University Rio de Janeiro Brazil

Stroke has been considered as one of the main causes of death and of motor and cognitive sequels. Especially, many patients with upper limb hemiparesis improved their motor action and showed meaningful cortical changes after treatment with constraint-induced movement therapy. Therefore, this review aims to verify the literature about neuroimaging and behavioral evidences in the cortical reorganization through the use of the constraint-induced movement therapy. So, we conducted the literature research in indexed journals from many databases like Pubmed, Medline, Cochrane Database, Lilacs and Scielo. We concluded that the behavioral and neuroimaging studies using traditional and modified constraint-induced movement therapy promote cortical reorganization.

关键词： Stroke Constraint-Induced-Movement-Therapy Neuroplasticity Cerebral Reorganization

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