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A grain-size correction for metal pollution indexes in river sediments

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International Journal of Sediment Research 2021年第3期36卷 362-372页

作者： Thomas Vincent Gloaguen Paula Núbia Soares Dalto Motta carolina Fonseca Couto Federal University of Reconcavo of Bahia Federal University of Minas Gerais

According to the List of Hazardous Substances of the Agency of the Toxic Substances and Disease Registry(ATSDR 2017), some metallic elements such as Pb or Cd are still considered as the most polluting elements in the world. These elements accumulate in sediments, and there are various methods available that differentiate lithogenic sources from anthropogenic sources. For that, the natural geochemical background is required, however, its definition is far from unanimous: it can be a global or local value,single or statistically estimated value, depending on the studies. Our study is focused on the Subae River in the state of Bahia, Northeastern Brazil, which was historically contaminated by lead metallurgy. The river sediments were sampled at 21 locations: one at the discharge point of the plant’s rainwater basin,ten upstream, and ten downstream. The total contents of Fe, Ti, Mn, Pb, Zn, Cu, Cr, particle-size distribution, and organic matter were analyzed. The conventional geochemical indexes, i.e., the pollution degree(m Cd), pollution load index, and enrichment factor indicated contamination by Zn, Pb, Cr, and ***, a new enrichment factor is introduced, assuming that the sediments come from erosion of soils in the watershed. After obtaining the natural concentrations of metals in the clay, silt, and sand fractions of uncontaminated reference soils, we corrected the pollution indexes by calculating a new individual background in each sediment sample, according to its grain size distribution. This new approach provided more precise indexes in the assessment of sediment pollution, by highlighting higher contamination of Zn and Pb(around 50%) and at the same time the absence of Cr and Cu contamination, two metals not involved with the metallurgical activity.

关键词： Enrichment factor Estimated background Particle-size distribution

Agrobacterium-mediated transformation and assessment of factors influ-encing transgene expression in loblolly pine （Pinus taeda L.）

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Cell Research 2001年第3期11卷 237-243页

作者： TANG WEI North carolina State University, 2900 Ligon Sf., Raleigh, NC 27607, USA North carolina State University Raleigh USA

This investigation reports a protocol for transfer and expression of foreign chimeric genes in loblolly pine (Pinus taeda L.). Transformation was achieved by co-cultivation of mature zygotic embryos with Agrobacterium tumefaciens strain LBA4404 which harbored a binary vector (pBI121) including genes for β-glucuronidase (GUS) and neomycin phosphotransferase (NPTII). Factors influencing transgene expression including seed sources of loblolly pine, concentration of bacteria, and the wounding procedures of target explants were investigated. The expression of foreign gene was confirmed by the ability of mature zygotic embryos to produce calli in the presence of kanamycin, by histochemical assays of GUS activity, by PCR analysis, and by Southern blot. The successful expression of the GUS gene in different families of loblolly pine suggests that this transformation system is probably useful for the production of the genetically modified conifers.

关键词： Pinus taeda L. Agrobacterum tumefaciens gene transfer gene expression.

COVID-19 pandemic in the intensive care unit:Psychological implications and interventions,a systematic review

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World Journal of Psychiatry 2023年第4期13卷 191-217页

作者： Laura Monti Elisa Marconi Maria Grazia Bocci Georgios Demetrios Kotzalidis Marianna Mazza carolina Galliani Sara Tranquilli Giovanni Vento Giorgio Conti Gabriele Sani Massimo Antonelli Daniela Pia Rosaria Chieffo UOS Psicologia Clinica Governo ClinicoFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Anestesia RianimazioneTerapia Intensiva e Tossicologia ClinicaDipartimento di Scienze Dell’EmergenzaAnestesiologiche e Della RianimazioneFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Psichiatria Clinica e d’Urgenza Dipartimento di Scienze Dell’InvecchiamentoNeurologicheOrtopediche e Della Testa-colloFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy NESMOS Department Facoltàdi Medicina e PsicologiaSant’Andrea University HospitalRome 00189Italy Department of Psychiatry UniversitàCattolica del Sacro CuoreRome 00168Italy Department of Psychology UniversitàCattolica del Sacro CuoreRome 00168Italy UOC Neonatologia Dipartimento di Scienze Della Salute Della DonnaDel Bambino e di SanitàPubblicaFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Neonatologia UniversitàCattolica del Sacro CuoreRome 00168Italy UOC Terapia Intensiva Pediatrica e Trauma Center Pediatrico Dipartimento di Scienze Dell’EmergenzaAnestesiologiche e Della RianimazioneFondazione Policlinico Universitario Agostino Gemelli IRCCSRome 00168Italy UOC Terapia Intensiva Pediatrica e Trauma Center Pediatrico UniversitàCattolica del Sacro CuoreRome 00168Italy Department of Anesthesiology and Intensive Care Medicine UniversitàCattolica del Sacro CuoreRome 00168Italy

BACKGROUND The coronavirus disease 2019(COVID-19) pandemic produced changes in intensive care units(ICUs) in patient care and health organizations. The pandemic event increased patients’ risk of developing psychological symptoms during and after hospitalisation. These consequences also affected those family members who could not access the hospital. In addition, the initial lack of knowledge about the virus and its management, the climate of fear and uncertainty, the increased workload and the risk of becoming infected and being contagious, had a strong impact on healthcare staff and organizations. This highlighted the importance of interventions aimed at providing psychological support to ICUs, involving patients, their relatives, and the staff;this might involve the reorganisation of the daily routine and rearrangement of ICU staff *** To conduct a systematic review of psychological issues in ICUs during the COVID-19 pandemic involving patients, their relatives, and ICU *** We investigated the PubMed and the *** databases and found 65 eligible articles,upon which we *** Our results point to increased perceived stress and psychological distress in staff, patients and their relatives and increased worry for being infected with severe acute respiratory syndrome coronavirus-2 in patients and relatives. Furthermore, promising results were obtained for some psychological programmes aiming at improving psychological measures in all ICU *** As the pandemic limited direct inter-individual interactions, the role of interventions using digital tools and virtual reality is becoming increasingly important. All considered, our results indicate an essential role for psychologists in ICUs.

关键词： COVID-19 Intensive care unit Psychological interventions Pandemic Mental health Health care professionals

Anti-inflammatory effects ofα-humulene andβ-caryophyllene on pterygium fibroblasts

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International Journal of Ophthalmology(English edition) 2022年第12期15卷 1903-1907页

作者： Magda Massae Hata Viveiros Márcia Guimarães Silva JoséGalberto Martins da Costa Anselmo Gomes de Oliveira carolina Rubio Carlos Roberto Padovani Cláudia Aparecida Rainho Silvana Artioli Schellini Department of Ophthalmology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 18618-000Brazil Department of Pathology School of Medicine of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil Department of Biological Chemistry Regional University of CaririCratoCeará66100-900Brazil Department of Pharmacology School of Pharmaceutical SciencesSão Paulo State University–UNESPAraraquaraSao Paulo 01049-010Brazil Biological Sciences Institute of Biosciences of BotucatuSão Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil Department of Biostatistics São Paulo State University–UNESPBotucatuSao Paulo 01049-010Brazil

AIM:To investigate the anti-inflammatory effects of the sesquiterpenesα-humulene andβ-caryophyllene on pterygium ***:Primary cultures of pterygium fibroblasts were *** passage pterygium fibroblasts were exposed toα-humulene andβ-caryophyllene separately and *** cell viability was assessed by 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide(MTT)assay at 12,24,48,and 72h after *** levels of the inflammatory cytokines interleukin(IL)-1β,IL-6,IL-8,tumor necrosis factor(TNF)-αand IL-10 in the conditioned culture medium were assessed by enzymelinked immunosorbent assay(ELISA)at 12,24 and 48h after *** were statistically analyzed using Friedman repeated measures analysis of variances on ***:The 25μmol/Lβ-caryophyllene induced significant decrease in the IL-6 production by pterygium fibroblasts 48h after the exposure(P=0.041).The levels of IL-1β,IL-8,IL-10,and TNF-αwere very low and had no statistically significant variations af ter exposure toα-humulene,β-caryophyllene,or both compounds ***:The exposure to 25μmol/L ofβ-caryophyllene significantly reduce the production of IL-6 by pterygium fibroblasts after *** sesquiterpene may be a potential alternative adjuvant agent for the treatment of pterygium.

关键词： pterygium fibroblasts β-caryophyllene α-humulene cytokines

Gene silencing:Double-stranded RNA mediated mRNA degradation and gene inactivation

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Cell Research 2001年第3期11卷 181-186页

作者： TANG WEI, XIAO YAN LUO, VANESSA SANMUELS North carolina State University, Forest Biotechnology Groap, Raleigh, NC 27695, USA University of North carolina, Department of Cell and Developmental Biology, ChaPel Hill, NC 27599, USA North carolina State University Forest Biotechnology Group Raleigh USA Department of Cell and Developmental Biology University of North Carolina Chapel Hill USA

The recent development of gene transfer approaches in plants and animals has revealed that transgene can undergo silencing after integration in the genome. Host genes can also be silenced as a consequence of the presence of a homologous transgene. More and more investigations have demonstrated that double- stranded RNA can silence genes by triggering degradation of homologous RNA in the cytoplasm and by directing methylation of homologous nuclear DNA sequences. Analyses of Arabidopsis mutants and plant viral suppressors of silencing are unraveling RNA-silencing mechanisms and are assessing the role of methy- lation in transcriptional and posttranscriptional gene silencing. This review will focus on double-stranded RNA mediated mRNA degradation and gene inactivation in plants.

关键词： Gene silencing double-stranded RNA methylation homologous RNA transgene.

Association of non-alcoholic fatty liver and metabolic-associated fatty liver with COVID-19 outcomes:A systematic review and metaanalysis

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World Journal of Gastroenterology 2023年第21期29卷 3362-3378页

作者： Gowthami Sai Kogilathota Jagirdhar Rakhtan K Qasba Harsha Pattnaik Kaanthi Rama Akshat Banga Shiva Teja Reddy Anna carolina Flumignan Bucharles Rahul Kashyap Praveen Reddy Elmati Vikas Bansal Yatinder Bains Theodore DaCosta Salim Surani Department of Medicine Saint Michaels Medical CenterNewarkNJ 07102United States Department of Medicine Green Life Medical College and HospitalDhaka 1205Bangladesh Department of Medicine Lady Hardinge Medical CollegeNew Delhi 110001India Department of Medicine Gandhi Medical CollegeTelangana 500003India Department of Medicine Sawai Man Singh Medical CollegeJaipur 302004RajistanIndia Department of Medicine Universidade PositivoCuritiba 81290-000PRBrazil Research WellSpan HealthYorkPA 17403United States Department of Interventional Pain Medicine University of LouisvilleLouisvilleKY 40292United States Department of Internal Medicine Mayo ClinicRochesterMN 55902United States Department of Gastroenterology Saint Michaels Medical CenterNewarkNJ 07102United States Department of Medicine Texas A&M UniversityCollege StationTX 77843United States

BACKGROUND Non-alcoholic fatty liver disease(NAFLD)and metabolic-associated fatty liver disease(MAFLD)are on the rise like any other liver disease,and tend to affect 25%of the United States *** impact of NAFLD and MAFLD on patients with coronavirus disease 2019(COVID-19)remains *** To identify the association of NAFLD and MAFLD with mortality,hospitalization,hospital length of stay,and supplemental oxygen utilization in COVID-19 *** A systematic review of literature on Cochrane,Embase,PubMed,ScienceDirect,and Web of Science databases was conducted from January 2019 to July *** that evaluated NAFLD/MAFLD using laboratory methods,noninvasive imaging,or liver biopsy were *** study protocol was registered in PROSPERO(ID CRD42022313259)and PRISMA guidelines were *** National Institutes of Health quality assessment tool was used to assess the quality of the *** analysis was conducted using software Rev Man version *** stability of the results was assessed using sensitivity *** Thirty-two studies with 43388 patients were included in the meta-analysis of whom 8538(20%)patients were observed to have *** were 42254 patients from 28 studies included in the mortality analysis.A total of 2008 patients died from COVID-19;837(10.52%)in the NAFLD group and 1171(3.41%)in the non-NAFLD *** odds ratio(OR)was 1.38 for mortality with a 95%confidence interval(95%CI)=0.97-1.95 and P=0.07.A total of 5043 patients from eight studies were included in the hospital length of stay *** were 1318 patients in the NAFLD group and 3725 patients in the non-NAFLD group.A qualitative synthesis showed that the mean difference in hospital length of stay was about 2 d between the NAFLD and non-NAFLD groups with a 95%CI=0.71-3.27 and P=*** hospitalization rates,the OR was 3.25 with a 95%CI of 1.73-6.10 and P=*** supplemental oxygen utilization,the OR was 2.04 with a 95%CI of 1.17-3.5

关键词： Non-alcoholic fatty liver Fatty liver Coronavirus COVID-19 Metabolic-associated fatty liver

Investigation of different pre-treatments of chromium leather shavings to improve biogas production

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Journal of Leather Science and Engineering 2020年第1期2卷 146-159页

作者： carolina Scaraffuni Gomes Jens-Uwe Repke Michael Meyer Forschungsinstitut für Leder und Kunststoffbahnen(FILK)gGmbH MeiBner Ring 1-509599 FreibergGermany Dynamik und Betrieb technischer Anlagen TU BerlinStraße des 17.Juni 13510623 BerlinGermany

Chromium shavings are wet by-products of the leather industry,of which thousands of tons accrue worldwide during leather *** to their chromium content,chromium shavings are responsible for the most important ecological challenges caused by the leather *** are stable towards temperatures of up to 110°C and enzymatic *** high stability is caused by the three-dimensional native structure,typical for collagen,and additional chemical cross-links between the collagen fibres achieved by Cr3+salts in the tanning ***,hitherto chromium shavings are not utilized industrially to produce *** order to generate biogas,this stable structure has to be *** shavings were pre-treated by extrusion and hydrothermal *** prove the enzymatic degradability,the different pre-treatments were evaluated by differential scanning calorimetry(DSC),enzymatic assays,and by measuring the solubility in *** biogas production potential was investigated using batch trials to examine *** demonstrated that both pretreatments allowed enzymatic attack and increased degradability of the chromium shavings reducing the lag-phase of biogas production and the remaining waste.

关键词： Biogas production Anaerobic digestion Chromium shavings Extrusion Hydrothermal treatment

Human bocavirus in children hospitalized for acute respiratory tract infection in Rome

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World Journal of Pediatrics 2020年第3期16卷 293-298页

作者： Laura Petrarca Raffaella Nenna Antonella Frassanito Alessandra Pierangeli Greta Di Mattia carolina Scagnolari Fabio Midulla Maternal-Infantile and Urological Science Department "Sapienza"University of RomeRomeItaly Virology Laboratory Department of Molecular Medicine"Sapienza"University of RomeRomeItaly

Background The role of human bocavirus (HBoV) as a respiratory pathogen has not been fulfilled *** aimed to describe clinical and serological characteristics of children with HBoV hospitalized for acute respiratory tract infection and to evaluate whether differences occur between HBoV alone and in *** We retrospectively reviewed data from 60 children (median age of 6.2 months,range 0.6-70.9) hospitalized for acute respiratory symptoms,with HBoV detected from a respiratory sample,using a reverse transcriptase-PCR for 14 respiratory viruses (including respiratory syncytial virus (RSV),influenza virus A and B,human coronavirus OC43,229E,NL-63 and HUK1,adenovirus,rhinovirus,parainfluenza virus 1-3,and human metapneumovirus).Results HBoV was detected alone in 29 (48.3%) patients,while in co-infection with other viruses in 31 patients (51.7%),with a peak between December and *** the 60 patients,34 were bronchiolitis,19 wheezing,3 pneumonia,2 upper respiratory tract infection,and 2 whooping *** children (11.6%) required admission to the paediatric intensive care unit (PICU) for respiratory *** differences was observed in age,family history for atopy and/or asthma,clinical presentations,chest X-ray,or laboratory findings in children with HBoV alone *** viral *** was the most frequently co-detected virus (61.3%).When compared with HBoV detection alone,the co-detection of RSV and HBoV was associated with male sex (P=0.013),younger age (P=0.01),and lower blood neutrophil count (P=0.032).Conclusions HBoV can be detected alone and in co-infection respiratory samples of children with an acute respiratory tract infection.A cause-effect relationship between HBoV and respiratory infection is not clear,so further studies are needed to clarify this point.

关键词： Human bocavirus Pediatrics Respiratory tract infection Viral infection

Essential amino acid ratios and mTOR affect lipogenic gene networks and miRNA expression in bovine mammary epithelial cells

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Journal of Animal Science and Biotechnology 2016年第4期7卷 612-622页

作者： Shanshan Li Afshin Hosseini Marina Danes carolina Jacometo Jianxin Liu Juan J.Loor Institute of Dairy Science College of Animal SciencesZhejiang University Department of Animal Sciences Mammalian Nutri Physio GenomicsUniversity of Illinois Department of Animal Science University of Lavras NUPEEC Departamento de Clínicas VeterináriaPrograma de Pós-Gradua??o em BiotecnologiaUniversidade Federal de Pelotas

Background： The objective of this study was to study how changing the ratio of Lys to Thr, Lys to His, and Lys to Val affects the expression of lipogenic genes and microRNA （miRNA） in bovine mammary epithelial cells. Results： Triplicate cultures with the respective ＂optimal＂ amino acid （AA） ratio （OPAA = Lys：Met 2.9：1; Thr：Phe 1.05：1; Lys：Thr 1.8：1; Lys：His 2.38：1; Lys：Val 1.23：1） plus rapamycin （OPAARMC; positive control）, OPAA, Lys：Thr 2.1：1 （LT2.1）, Lys：Thr 1.3：1 （LT1.3）, Lys：His 3.05：1 （LH3.0）, or Lys：Val 1.62：1 （LV1.6） were incubated in lactogenic medium for 12 h. The expression of 15 lipogenic genes and 7 miRNA were evaluated. Responses to LT2.1, LT1.3, LH3.0, and LV1.6 relative to the control （OPAARMC） included up-regulated expression ofACSS2, FABP3, ACACA, FASN, SCD, LPIN1, INSIG1, SREBF1, PPARD, and NR1H3 （commonly known as LXR-a）. Furthermore, LV1.6 up-regulated expression of ACSL1, DGAT1, and RXRA and down-regulated PPARG expression. Although no effect of OPAA on expression of PPARG was observed, compared with the control, OPAA up-regulated expression of the PPAR targets ACSS2, FABP3, ACACA, FASN, SCD, LPIN1, INSIG1, and SREBF1. Compared with the control, the expression of the anti-lipogenic MIR27AB was down-regulated by OPAA, LT2.1, LT1.3 and LH3.0. In contrast, compared with the control, the expression of the pro-lipogenic MIR21 was up-regulated by LT2.1, LT1.3, LH3.0, and LV1.6. Conclusions： The observed up-regulation of lipogenic gene networks and the changes in expression of key miRNA involved in the control of lipogenic balance are indicative of a potentially important role of EAA ratios and mTOR signaling in the regulation of milk fat synthesis.

关键词： Amino acids Milk fat synthesis mTOR Nutrigenomics

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