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Dietary carbohydrate-to-protein ratio influences growth performance,hepatic health and dynamic of gut microbiota in atlantic salmon(Salmo salar)

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Animal Nutrition 2022年第3期10卷 261-279页

作者： Alejandro Villasante carolina Ramírez Hector Rodríguez Patricio Dantagnan Adrian Hernandez Elías Figueroa Jaime Romero Facultad de Medicina Veterinaria y Agronomía Universidad de Las AmericasSantiagoChile Laboratorio de Biotecnología de Alimentos Unidad de AlimentosInstituto de Nutricion y Tecnología de Los Alimentos(INTA)Universidad de ChileSantiagoChile Programa de Anatomía y Biología Del Desarrollo Facultad de MedicinaUniversidad de ChileSantiagoChile Núcleo de Investigacion en Produccion Alimentaria Facultad de Recursos NaturalesUniversidad Catolica de TemucoTemucoChile

Atlantic salmon(Salmo salar)fed a carbohydrate-rich diet exhibit suboptimal growth performance,along with other metabolic *** is well known that gut microbes play a pivotal role in influencing metabolism of the host,and these microbes can be modified by the *** main goal of the present study was to determine the effect of feeding graded levels of digestible carbohydrates to Atlantic salmon on the distal intestine digesta microbiota at 3 sampling times(i.e.,weeks 4,8 and 12),during a 12-week trial.A low carbohydrate-to-high protein diet(LC/HP,0%wheat starch),a medium carbohydrate-tomedium protein diet(MC/MP,15%wheat starch)or a high carbohydrate-to-low protein diet(HC/LP,30%wheat starch)was fed to triplicate fish tanks(27 to 28 fish per tank).We performed an in-depth characterization of the distal intestine digesta ***,growth parameters,liver histology and the expression of genes involved in hepatic neolipogenesis in fish were *** fed a HC/LP diet showed greater hepatosomatic and viscerosomatic indexes(P=0.026 and P=0.018,respectively),lower final weight(P=0.005),weight gain(P=0.003),feed efficiency(P=0.033)and growth rate(P=0.003)compared with fish fed the LC/HP ***,feeding salmon a high digestible carbohydrate diet caused greater lipid vacuolization,steatosis index(P=0.007)and expression of fatty acid synthase(fas)and delta-6 fatty acyl desaturase(d6fad)(P=0.001 and P=0.001,respectively)in the liver compared with fish fed the LC/HP ***,the major impact of feeding a carbohydrate-rich diet to Atlantic salmon in beta diversity of distal intestine digesta microbiota was observed at week 4(HC/LP vs MC/MP and HC/LP vs LC/HP;P=0.007 and P=0.008,respectively)and week 8(HC/LP vs MC/MP;P=0.04),no differences between experimental groups were detected after 12 weeks of ***,at the end of the trial,there was a negative correlation between lactic acid bacteria(LAB)members,including Leuconostoc and Lactobacil

关键词： Microbiota Microbiome Atlantic salmon Steatosis Lactic acid bacteria Probiotics

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Mass transfer and loss of the massive semi-detached binary AI Crucis

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Research in Astronomy and Astrophysics 2010年第5期10卷 438-444页

作者： Er-Gang Zhao Sheng-Bang Qian E. Femanindez Lajus carolina von Essen Li-Ying Zhu National Astronomical Observatories / Yunnan Observatory Chinese Academy of SciencesKunming 650011 China Key Laboratory for the Structure and Evolution of Celestial Objects Chinese Academy ofSciences Kunming 650011 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Facultad de Ciencias Astron6micas y Geoffsicas Universidad Nacional de La Plata 1900 LaPlata Buenos Aires Argentina

AI Crucis is a short-period semi-detached massive close binary （P = 1.41771d, Sp.=B1.5） in the open cluster NGC 4103. It is a good astrophysical laboratory for investigating the formation and evolution of massive close binary stars via case A mass transfer. Orbital period variations of the system were analyzed based on one newly determined eclipse time and the others compiled from the literature. It is discovered that the orbital period of the binary is continuously increasing at a rate of dP/dt = ＋1.00（±0.04） × 10-7 d yr-1. After the long-term increase is subtracted from the O - C diagram, weak evidence indicates the presence of a cyclic oscillation with a period of 30.1yr, which may reveal a very cool stellar companion in the system. The long-term period increase can be explained by mass transfer from the less massive component to the more massive one. This is in agreement with the semidetached configuration of the binary, indicating that the system is undergoing a slow mass-transfer stage on the nuclear time scale of the secondary. However, it is found that the slow mass transfer is insufficient to cause the observed period increase, which suggests that the stellar wind from the hot component should contribute to the amount of period increase dP/dt = ＋0.54× 10-7 d yr-1 that corresponds to a mass loss rate of M˙1 = 2.72 × 10-7 M yr-1. It is estimated that the hot component lost a total mass of 4.1M during the slow mass-transfer stage and, thus, the evolution of the binary system should be changed greatly by the mass loss.

关键词： stars: binaries: close — stars: binaries: eclipsing — stars: individual (AI Crucis) — stars: early-type — stars: evolution

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Atacama Field Campaign: laboratory and in-situ measurements for remote sensing applications

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International Journal of Digital Earth 2019年第1期12卷 43-61页

作者： Cristian Mattar Andrés Santamaría-Artigas Flavio Ponzoni Cibele T.Pinto carolina Barrientos Glynn Hulley Laboratory for Analysis of the Biosphere(LAB) University of ChileSantiagoChile Department of Geographical Sciences University of MarylandCollege ParkMDUSA Divisão de Sensoriamento Remoto(DSR) Instituto Nacional de Pesquisas Espaciais(INPE)São PauloBrasil Aerial Photogrammetric Service(SAF) Chilean Air ForceSantiagoChile Jet Propulsion Laboratory(JPL) California Institute of TechnologyPasadenaCAUSA

This work presents the preliminary results of the first field calibration campaign performed in the Atacama Desert,Chile,between the 18 and 22 August 2014,called the Atacama Field Campaign(ATAFIC 2014).In situ measurements were performed in order to spectrally characterize the surface reflectance spectra between 0.3 and 2.5μm,radiometric temperature(8.0–14.0μm)and atmospheric measurements.A soil sample was collected and analyzed using Fourier Transform Infrared Spectroscopy and X-Ray Diffraction techniques to characterize the surface reflectance spectra and mineralogical composition,*** land surface emissivity in addition to GOES,MODIS and Landsat-8 land surface temperature(LST)were also *** showed that the spectral features of the Atacama soil and the characteristics of this geographical zone,which is featured as the most hyper-arid and cloudless place in the world,make this area a potential target for surface reflectance *** and night LST comparison between field and remote sensing data are lower than 2 K and the Root Mean Square Error for land surface emissivity is close to 2%.This work opens the possibilities to consider the Atacama Desert as a reference target for calibration and validation activities for earth observation missions’purposes.

关键词： Atacama spectroradiometry emissivity temperature surface reflectance

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Innovative strategies and nutritional perspectives for fortifying pumpkin tissue and other vegetable matrices with iron

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Food Science and Human Wellness 2020年第2期9卷 103-111页

作者： Marina F.de Escalada Pla Silvia K.Flores carolina E.Genevois Universidad de Buenos Aires Facultad de Ciencias Exactas y NaturalesDepartamento de IndustriasBuenos AiresArgentina CONICET-Universidad de Buenos Aires Instituto de Tecnologia de Alimentos y Procesos Quimicos(ITAPROQ)Buenos AiresArgentina Universidad Nacional de Entre Rios(UNER) Facultad de BromatologiaGualeguaychuEntre RiosArgentina CONICET Buenos AiresArgentina

The present review article focuses on different technological strategies and nutritional perspectives having added advantage to human health in fortifying vegetables matrices with *** introduction to the main aspects related to iron deficiency consequences is resumed in order to understand the importance of developing new strategies for improving iron *** this sense,the tendencies and alternatives will also be *** technologies like impregnation or dry infusion are presented as sustainable options for obtaining structured fortified vegetables.A review about the usefulness of edible covers for stabilizing micronutrients and/or probiotic microorganisms in fortified matrices is also *** iron deficiency still continue to be a worldwide health issue,innovating in food fortification remains a challenge for researchers and food manufactures.

关键词： Vegetables Iron Ascorbic acid Probiotics Edible covers

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Ornithine aspartate effects on bacterial composition and metabolic pathways in a rat model of steatotic liver disease

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World Journal of Hepatology 2024年第5期16卷 832-842页

作者： Elisa carolina Lange Pabulo Henrique Rampelotto Larisse Longo Laura Bainy Rodrigues de Freitas carolina Uribe-Cruz Mario Reis Alvares-da-Silva Experimental Laboratory of Hepatology and Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007Brazil Graduate Program in Gastroenterology and Hepatology Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Bioinformatics and Biostatistics Core Facility Universidade Federal do Rio Grande do SulPorto Alegre 91501-970Brazil Graduate Program in Biological Sciences:Pharmacology and Therapeutics Universidade Federal do Rio Grande do SulPorto Alegre 90035-007Brazil Facultad de Ciencias de la Salud Universidad Católica de las MisionesPosadasMisiones 3300Argentina Division of Gastroenterology Hospital de Clínicas de Porto AlegrePorto Alegre 90035-007RSBrazil Conselho Nacional de Desenvolvimento Científico e Tecnológico(CNPq)researcher Brasília 71605-001Brazil

BACKGROUND Metabolic-dysfunction associated steatotic liver disease(MASLD)is a hepatic manifestation of metabolic *** suggest ornithine aspartate(LOLA)as drug *** To analyze the influence of LOLA intake on gut microbiota using a nutritional model of *** Adult male Sprague Dawley rats were randomized into three groups:Control(10 rats fed with a standard diet),MASLD(10 rats fed with a high-fat and choline-deficient diet),and LOLA(10 rats receiving 200 mg/kg/d LOLA,after the 16th week receiving high-fat and choline-deficient diet).After 28 wk of the experiment,animals were euthanized,and feces present in the intestine were *** fecal DNA extraction,the V4 region of the 16S rRNA gene was amplified followed by sequencing in an Ion S5™*** Alpha and beta diversity metrics were comparable between MASLD and LOLA.3 OTUs were differentially abundant between MASLD and LOLA,which belong to the species Helicobacter rodentium,Parabacteroides goldsteinii,and Parabacteroides *** functional prediction provided two different metabolic profiles between MASLD and *** 9 pathways differentially abundant in MASLD are related to a change in energy source,adenosine/purine nucleotides degradation as well as guanosine and adenosine deoxyribonucleotides *** 14 pathways differentially abundant in LOLA are associated with four major metabolic functions primarily influenced by L-aspartate,including tricarboxylic acid cycle pathways,purine/guanosine nucleotides biosynthesis,pyrimidine ribonucleotides biosynthesis and salvage as well as lipid IVA *** Although LOLA had no influence on alpha and beta diversity in this nutritional model of MASLD,it was associated with changes in specific gut microbes and their related metabolic pathways.

关键词： Animal model Gut microbiota Metabolic-associated steatotic liver disease Metabolic prediction Ornithine aspartate

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The peptide fraction of Bothrops jararaca snake venom induces toxicological effects on the male reproductive system after local envenomation in mice

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BIOCELL 2023年第2期47卷 289-295页

作者： CARLOS ALBERTO-SILVA ANA carolina DE ARAUJO RODRIGO SIMÃO BONFIM JOYCE MEIRE GILIO Natural and Humanities Sciences Center Experimental Morphophysiology LaboratoryFederal University of ABC(UFABC)São Bernardo do Campo09606-070Brazil Departamento de Biofísica Universidade Federal de São PauloSão Paulo04023-062Brazil

Bothrops envenomation is complex and provokes prominent local tissue damage and systemic disturbances,but little is known about their effects on the male reproductive *** intratesticular injection,the bioactive peptide fraction(Bj-PF)obtained from Bothrops jararaca snake venom changes the structure of different stages of the seminiferous epithelium cycle in adult *** the first time,we investigated whether local envenomation of Bj-PF induces toxicological effects on the male reproductive system,particularly on the seminiferous epithelium and Sertoli *** adult mice were treated with 0.24 ***^(-1) by intramuscular(i.m.)injection for 24 *** testes samples were collected for morphological and morphometric *** toxicological effects of Bj-PF were also analyzed on mitochondrial metabolism and nitrite(NO2)production in 15P-1 Sertoli cell ***-PF changed the structure and function of the seminiferous epithelium,particularly the disruption of the epithelium and the presence of degenerated germ cells in the adluminal compartment,but there were no alterations in the basal ***-PF increased the thickness of the seminiferous epithelium and decreased the lumen diameter of the *** histological assessment of the degree of tubule degeneration revealed that Bj-PF also increased the number of hypospermatogenic tubules compared to ***-PF reduced NO2 levels in 15P-1 Sertoli cells without changing the mitochondrial ***,the fact that Bj-PF alters the structure and function of the seminiferous epithelium suggests that bioactive peptides found in *** snake venom can have toxicological effects on the reproductive systems of affected male mice,providing new insight into the biological characteristics of snake venom and therapeutic strategies for envenomation inflammation.

关键词： Snake venom Bothrops jararaca Reproductive toxicology Seminiferous epithelium

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Comparison of grain protein profiles of Brazilian cowpea (Vigna unguiculata) cultivars based on principal component analysis

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Food Production, Processing and Nutrition 2022年第1期4卷 172-182页

作者： Tuany Camila Honaiser Gabriela Barbosa Rossi Maurisrael de Moura Rocha Ana carolina Maisonnave Arisi Food Science and Technology Department CAL CCA UFSCFederal University of Santa CatarinaRod.Admar GonzagaFlorianópolisSanta Catarina134688034-001Brazil Embrapa Meio-Norte Caixa Postal 001TeresinaPiauí64008-780Brazil

This study aims to compare the grain protein profile of four Brazilian cowpea cultivars (BRS Aracê, BRS Itaim, BRS Pajeú, and BRS Xiquexique) by two-dimensional electrophoresis (2-DE) and principal component analysis (PCA). 2-DE efficiently separate cowpea protein profiles, showing high homogeneity among the four cultivars. In addition, the principal component analysis indicated that there is a difference in abundance of proteins among the cultivars. The cultivars BRS Aracê and BRS Xiquexique, both biofortified in iron and zinc, were separated from the cultivars BRS Itaim and BRS Pajeú. These results demonstrate that protein profiles can be used to discriminate cowpea varieties.

关键词： Vigna 2-DE PCA Legumes

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Appropriate search techniques to estimate Weibull function parameters in a Pinus ***

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Journal of Forestry Research 2021年第6期32卷 2423-2435页

作者： Lais Almeida Araujo Rafael Menali Oliveira Mario Dobner Jr carolina Souza Jarochinskie Silva Lucas Rezende Gomide Department of Forest Sciences Federal University of LavrasLavrasMinas GeraisBrazil Department of Agriculture Biodiversity and ForestsFederal University of Santa CatarinaCuritibanosSanta CatarinaBrazil

The Weibull function,a continuous probability distribution,is widely used for diameter distribution modelling,in which parameter estimation performance is affected by stand attributes and fitting *** Weibull cumulative distribution function is nonlinear,and classical fitting methods may provide a not optimal *** the use of artificial intelligence by metaheuristics is reasonable for this optimisation ***,aimed and compared(1)the metaheuristics genetic algorithm and simulated annealing performance over the moment and percentile methods;(2)the hybrid strategy merging the metaheuristics tested and the percentile method and,(3)the metaheuristics fitness functions under basal area and density errors.A long-term experiment in a Pinus taeda stand subjected to crown thinning was *** to our findings,all methods have a similar performance,independent of the thinning regimes and *** pattern of the estimated parameters tends to be acceptable,as b increases over time and c increases after ***,our findings suggest that methods based on metaheuristics have a higher precision than classical methods for estimating Weibull *** to the classification test,the methods that involved simulated annealing stood *** hybrid method involving this metaheuristic also stood out,with accurate *** methods showed the poorest performance,and we therefore suggest the use of simulated annealing due to its faster processing time and high-quality solution.

关键词： Forest management Diameters distribution Weibull function Genetic algorithm Simulated annealing

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Eucalyptus grandis plantations:effects of management on soil carbon,nutrient contents and yields

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Journal of Forestry Research 2020年第2期31卷 601-611页

作者： Diego Martin Sandoval Lopez Marcelo Fabian Arturi Juan Francisco Goya carolina Alejandra Perez Jorge Luis Frangi Laboratorio de Investigacion de Sistemas Ecologicos y Ambientales(LISEA) Universidad Nacional de La PlataDiagonal 113 N°4691900 La PlataBuenos AiresArgentina

The expansion of fast-growing tree plantations is a worldwide process,with consequences on soil fertility and soil carbon *** results were found on the effects of afforestation with Eucalyptus on soil carbon and other nutrient *** discrepancies are usually caused by differences in climate,land use history,soil texture as well as by management related factors such as plantation age,number of rotations,method of establishment(plantation or coppice),harvest residue management and soil *** studied the effect of plantation age,number of rotations,and method of establishment on soils and plant nutrient concentrations in Eucalyptus grandis plantations in NE Argentina on different textured *** also determined if yields changed with nutrient variations in soils,and compared soils under plantations to soils under grasslands they ***-one *** stands of different ages,number of rotations and method of establishment were evaluated as well as eight grassland *** of carbon,nitrogen,phosphorus,potassium,calcium and magnesium were determined for soils and *** carbon and nitrogen decreased over the number of rotations and were more pronounced in soils with 50-60%sand than soils with>75%*** stands showed higher soil carbon and nitrogen levels than plantations,suggesting a negative effect of site preparation before planting on soil nutrient conservation,especially in fine-textured *** nutrient concentrations did not follow the trends observed for soil nutrients nor did they reflect nutrient *** was no evidence of decreased yields over successive *** carbon and nitrogen contents decrease when grasslands are replaced by *** plantations,and therefore a yield limitation may occur in a medium to long-term frame,especially in stands re-established for short-rotation *** residue management and site preparation must be specifically designed for improving so

关键词： Soil texture Grasslands Foliar nutrient concentrations Successive harvest Coppice Eucalyptus grandis

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Mesial temporal sclerosis and epilepsy:a narrative review

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Acta Epileptologica 2024年第3期7卷 178-188页

作者： Daniel Villamizar-Torres Andrea carolina Cepeda Trillos Alejandro Vargas-Moreno Member of the neurosurgery research group Pontificia Universidad JaverianaBogotá110231Colombia Radiology deparment Hospital Universitario San IgnacioBogotá110231Colombia Member of the epilepsy research group Pontificia Universidad JaverianaBogotá110231Colombia Neurosurgery department Hospital Universitario San IgnacioBogotá110231Colombia

Mesial temporal sclerosis(MTS)stands out as a prevalent etiology of medically intractable temporal lobe *** the pathological alterations,clinical manifestations and risk factors of MTS is crucial for the recognition and suspicion of this *** this paper,we provide a comprehensive narrative review on the pathophysiology,clinical manifestations,and treatment options for *** doing so,we aim to provide an up-to-date understanding of this condition.

关键词： Hippocampal sclerosis Epilepsy Temporal lobe Hippocampus

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