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Development of a chromatographic lateral flow immunoassay for detection of African swine fever virus antigen in blood

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Animal Diseases 2022年第3期2卷 139-153页

作者： Daniel W.Madden Sun-Young Sunwoo Natasha N.Gaudreault Jessie D.Trujillo Igor Morozov carmina Gallardo Juergen A.Richt Department of Diagnostic Medicine/Pathobiology College of Veterinary MedicineKansas State UniversityManhattanKSUSA Centro de Investigación en Sanidad Animal(CISA-INIA/CSIC) MadridSpain

African swine fever(ASF)is a highly lethal disease of domestic and wild swine caused by African swine fever virus(ASFV).The disease currently circulates in Africa,Europe,Asia and on the island of Hispaniola.The ongoing epizootics in Europe and Asia have produced millions of animal deaths and severe economic losses.No effective vaccine is available for ASF,making rapid and accurate detection of ASFV essential for disease mitigation strategies.Currently available diagnostics for ASFV possess significant limitations related to assay performance,deployability,and/or turn-around time;therefore there is an unmet need for pen-side diagnostic tests with sufficient sensitivity and specificity.A chromatographic lateral flow immunoassay(lFIA)was developed for the detection of ASFV antigen in EDTA-treated whole blood using monoclonal antibodies targeting the viral p30 protein.The assay requires only water to perform and provides results in 25 min,making it well-suited for field use.The lFIA was capable of detecting genotype I and genotype II strains of ASFV in EDTA blood from experimentally infected pigs at varying time-points after infection,though it was unable to detect a genotype X ASFV strain.Diagnostic sensitivity correlated with clinical disease severity,body temperature,and viral DNA levels,and was over 90%in animals showing moderate to severe ASF-related symptoms after challenge with virulent genotype II virus.The lFIA also showed a robust diagnostic specificity of over 98%,which is essential to field testing for a high consequence to foregin animal disease.The lFIA targeting the viral p30 protein can reliably detect ASFV in whole blood from animals showing moderate to severe clinical signs of infection with virulent genotype I and II isolates,making it a promising candidate for use as a field-deployable antigen detection assay.Additional evaluation using field samples and different virus strains is required to further assess the utility of this rapid diagnostic test.

关键词： African swine fever lateral flow immunoassay Point of care diagnostics

Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González luis Fernando lópez-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel lomas Claudio Bueno Rosario Amaya JoséAntonio lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan Delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-Delgado Manuel García-Gutiérrez María luisa Gascón-Castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-león Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia lanseros-Tenllado carmina lópez Isabel lópez Álvaro lópez-Barrios luis F.lópez-Cortés Rafael luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity components.Dendritic cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease severity.In this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory markers.Remarkably,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 infection.Moreover,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were observed.These findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell long-COVID

Neuroinflammation and neurological alterations in chronic liver diseases

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Neuroimmunology and Neuroinflammation 2015年第1期2卷 138-144页

作者： carmina Montoliu Marta llansola Vicente Felipo Research Foundation of the Clinic Hospital of Valencia Health Research Institute-INCLIVA46010 ValenciaSpain laboratory of Neurobiology Prince Felipe Research Center46012 ValenciaSpain

Several million people with chronic liver diseases(cirrhosis,hepatitis)show neurological alterations,named hepatic encephalopathy(HE)with cognitive and motor alterations that impair quality of life and reduces life span.Inflammation acts synergistically with hyperammonemia to induce cognitive and motor alterations in patients with chronic liver disease and minimal hepatic encephalopathy(MHE).Previous studies in animal models have suggested that neuroinflammation is a major player in HE.This would also be the case in patients with liver cirrhosis or hepatitis C with HE.Rats with MHE show microglial activation and neuroinflammation that is associated with cognitive impairment and hypokinesia.The anti-inflammatory drug ibuprofen reduces microglial activation and neuroinflammation and restores cognitive and motor functions in rats with MHE.Chronic hyperammonemia per se induces neuroinflammation.Both peripheral inflammation and hyperammonemia would contribute to neuroinflammation in chronic liver failure.Therefore,neuroinflammation may be a key therapeutic target to improve the cognitive and motor alterations in MHE and overt HE.Identifying new targets to reduce neuroinflammation in MHE without inducing secondary effects would serve to develop new therapeutic tools to reverse the cognitive and motor alterations in patients with HE associated with chronic liver diseases.

关键词： Cognitive impairment hepatic encephalopathy hyperammonemia inflammation motor function neuroinflammation

Diversity, Distribution and Composition of the Bivalvia Class on the Rocky Intertidal Zone of Marine Priority Region 32, Mexico

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Open Journal of Ecology 2014年第15期4卷 961-973页

作者： Rafael Flores-Garza Victor lópez-Rojas Pedro Flores-Rodríguez carmina Torreblanca Ramírez Unidad Académica de Ecología Marina Universidad Autónoma de Guerrero Acapulco Mexico Unidad de Ciencias de Desarrollo Regional Universidad Autónoma de Guerrero Acapulco Mexico

The Bivalvia Class, is widely represented in marine ecosystems and is economically important;this research focused on this class and was carried out by sampling the rocky intertidal zone of seven sites located in the Marina Region Priority No. 32, Guerrero, Mexico, where the National Commission for the Knowledge and Use of Biodiversity reported lack of knowledge on marine species living there. The objectives were to examine species richness associated with the rocky intertidal zone, determine how the community is composed by the representation of families based on species richness and abundance, know the distribution of the species, analyze the structure sizes of populations and estimate the diversity index. Sampling was conducted at seven sites;the sampling unit was one square meter and the sampling area was 10 m2. 32 species were identified. Four species are new records for the Mexican Pacific Transitional: one for the state of Guerrero and one for Marine Priority Region 32. Arcidae and Mytilidae Families were better represented in species richness and Chamidae Family was the best represented in abundance. Chama coralloides was considered to be the representative species of the area;Striostrea prismatica showed the greatest size. The diversity index was estimated (H' = 3.65 bits/individuals). Species richness is high and corresponds to that expected in a tropical area.

关键词： Bivalvia Distribution Intertidial Zone Guerrero Mexico

Augmented Reality:an Emerging Field in Pediatric Psycho-oncology Requiring Research

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Advances in Modern Oncology Research 2018年第6期4卷 1-4页

作者： carmina Castellano-Tejedor Andrés Cencerrado Psynaptic Psicología y Servicios Científicos y Tecnológicos S.L.P.Avenida Egara 3008192Sant Quirze del Vallès(Barcelona)Spain GIES Research Group Basic Psychology DepartmentUniversitat Autònoma de Barcelona08192Bellaterra(Barcelona)Spain RE-FiT BCN Research Group Parc Sanitari Pere Virgili&Vall d'Hebron Institute of Research08023Barcelona(Barcelona)Spain Computer Architecture and Operating Systems Department Universitat Autònoma de Barcelona08193Bellaterra(Barcelona)Spain

Augmented reality(onwards,AR)technologies are now much more complex and feature a higher number of details thanks to advancements in information and communication technologies(ICTs).Today’s systems can be easily adapted and packaged into smartphone apps which enable a wide range of applications in real and clinical practice.The impact on behavioral health treatments,rehabilitation and healthcare system in general is just beginning to become evident[1]but still,more research providing evidence-based practices is required.

关键词： details enable smart

Polymer Surface Roughness Determination throughout the Hurst Analysis from Optical Signal Measurements

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材料科学与工程（中英文版） 2010年第11期4卷 26-31页

作者： Antonio Marban Oscar Sarmiento-Martinez Darwin Mayorga-Cruz carmina Menchaca Jorge Uruchurtu Centro de Investigaciones en Ingenieria y Ciencias Aplieadas-CIICAp Av Universidad 1001 Chamilpa Cuernavaca MorelosMexico Universidad Autonoma Metropolitana- UAM Azcapotzalco Avenida del Rosario Azcapotzalco Mexico D.F.

Gamma-Irradiated Nylon Roughness as Function of Dose and Time by the Hurst and Fractal Dimension Analysis

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材料科学与工程（中英文版） 2010年第9期4卷 50-58页

作者： carmina Menchaca Juan Carlos Nava Sofia Valdez Oscar Sarmiento-Martinez Jorge Uruchurtu Centro de Investigacion en lngenieria y Ciencias Aplicadas （CllCAp） Universidad Autonoma del Estado de Morelos Cuernavaea Morelos 62209 Mexico

Otoacoustic Emissions and Evoked Potentials in Infants after Breast-Feeding Jaundice——Hearing Dysfunction in Breast-Feeding Jaundice

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Neuroscience & Medicine 2012年第3期3卷 270-274页

作者： Adrián Poblano Norma Ballesteros carmina Arteaga Blanca Flores Teodoro Flores laboratory of Clinical Neu-rophysiology National Institute of Rehabilitation Mexico City Mexico laboratory of Cognitive Neurophysiology National Institute of Rehabilitation Mexico City Mexico

We study hearing in a group of infants with Breast-feeding jaundice (BFJ) by means of Transient-evoked otoacoustic emissions (T-EOE) and Brainstem auditory evoked potentials (BAEP) searching for relationship between bilirubin serum levels and auditory dysfunction. Eleven infants born at-term with BFJ were selected for the study. We studied also 11 control age- and gender matched healthy at-term infants without signs of jaundice. T-EOAE studies were performed between 5-7 days after birth, and 3 months later. BAEP studies were performed once. BFJ group infants exhibited lower amplitudes in T-EOE than infants in the control group. These differences disappear at the 3-month evaluation. In BAEP, we observed a significant latency delay of waves I and V in Breast-feeding jaundice group infants. All infants in both groups demonstrated reproducible wave V response at 30 decibels. No significant correlation values were observed between bilirubin serum levels and T-EOE and BAEP variables. Our data suggest that BFJ can result in transient peripheral and central auditory dysfunction. Dysfunction is reversible after treatment of infants with BFJ.

关键词： Breast-Feed Jaundice Hyperbilirubinemia Hearing Neonates Transient-Evoked Otoacoustic Emissions Brainstem Auditory Evoked Potentials

Grafting vigour is associated with DNA de-methylation in eggplant

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Horticulture Research 2021年第1期8卷 3311-3320页

作者： Elisa Cerruti carmina Gisbert Hajk-Georg Drost Danila Valentino Ezio Portis lorenzo Barchi Jaime Prohens Sergio lanteri Cinzia Comino Marco Catoni Department of Agricultural Forest and Food SciencesPlant Genetics and BreedingUniversity of TorinoGrugliascoItaly The Sainsbury laboratory University of CambridgeCambridgeUK Institute for Conservation&Improvement of Valencian Agrodiversity(COMAV) Universitat Politècnica de ValènciaValenciaSpain Computational Biology Group Department of Molecular BiologyMax Planck Institute for Developmental BiologyTübingenGermany School of Biosciences University of BirminghamBirminghamUnited Kingdom Institute for Sustainable Plant Protection National Research Council of ItalyTorinoItaly

In horticulture,grafting is a popular technique used to combine positive traits from two different plants.This is achieved by joining the plant top part(scion)onto a rootstock which contains the stem and roots.Rootstocks can provide resistance to stress and increase plant production,but despite their wide use,the biological mechanisms driving rootstock-induced alterations of the scion phenotype remain largely unknown.Given that epigenetics plays a relevant role during distance signalling in plants,we studied the genome-wide DNA methylation changes induced in eggplant(Solanum melongena)scion using two interspecific rootstocks to increase vigour.We found that vigour was associated with a change in scion gene expression and a genome-wide hypomethylation in the CHH context.Interestingly,this hypomethylation correlated with the downregulation of younger and potentially more active long terminal repeat retrotransposable elements(lTR-TEs),suggesting that graft-induced epigenetic modifications are associated with both physiological and molecular phenotypes in grafted plants.Our results indicate that the enhanced vigour induced by heterografting in eggplant is associated with epigenetic modifications,as also observed in some heterotic hybrids.

关键词： younger driving grafted