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residual activity of cetrimide and chlorhexidine on Enterococcus faecalis-infected root canals

International Journal of Oral Science 2014年第1期6卷 46-49页

作者： carme. maría fe.re.-luque.maría teresa arias-moliz Matilde Ruíz-Linares maría Elena martínez García Pilar Baca Department of Stomatology School of Dentistry University of Granada Department of Microbiology School of Dentistry University of Granada

Effective final irrigation regimen is an important step in order to achieve better disinfection and ensure residual antimicrobial effects after root canal preparation. The aim of this study was to compare the residual antimicrobial activity of 0.2% cetrimide, and 0.2% and 2% chlorhexidine in root canals infected with Enterococcus faecalis. Biofilms of E. faecalis were grown on uniradicular roots for 4 weeks. After root canal preparation, root canals were irrigated with 17% ethylenediaminetetraacetic acid （EDTA） to remove the smear layer. The roots were randomly divided into three experimental groups （n=26） according to the final irrigating solution： Group I, 5 mL 0.2% cetrimide; Group II, 5 mL 0.2% chlorhexidine; and Group III, 5 mL 2% chlorhexidine. Samples were collected for 50 days to denote the presence of bacterial growth. The proportion of ungrown specimens over 50 days was evaluated using the nonparametric Kaplan-Meier survival analysis. Differences among groups were tested using the log-rank test and the level of statistical significance was set at P〈0.05. The highest survival value was found with 2% chlorhexidine, showing statistically significant differences from the other two groups. At 50 days, E. faecalisgrowth was detected in 69.23% specimens in Groups I and II, and in 34.61% specimens of Group III. There were no significant differences between 0.2% cetrimide and 0.2% chlorhexidine. Final irrigation with 2% chlorhexidine showed greater residual activity than 0.2% chlorhexidine and 0.2% cetrimide in root canals infected with E. faecalis.

关键词： antimicrobial effects biofilms cetrimide chlorhexidine Enterococcus faecalis final irrigation residual activity

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Antimicrobial activity of alexidine alone and associated with N-acetylcysteine against Enterococcus faecalis biofilm

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International Journal of Oral Science 2013年第3期5卷 146-149页

作者： Luiz fernando Machado Silveira Pilar Baca maría teresa arias-moliz Alberto Rodríguez-Archilla carme. maría fe.re.-luque Department of Semiology and Clinics College of Dentistry Federal University of Pelotas Department of Stomatology School of Dentistry University of Granada Department of Microbiology School of Dentistry University of Granada

The purpose of this study was to assess the efficacy of alexidine（ALX）,alone and combined with N-acetylcysteine（NAC）,in eradicating two Enterococcus faecalis strain *** biofilms of *** ATCC 29212 and the clinical isolate *** D1 were grown in the MBEC-high-throughput device for 24 h and were exposed to five twofold dilutions of ALX（2%–0.007 8%）alone and combined with100 mg？mL21NAC,for 1 and 5 *** was defined as 100%kill of biofilm *** Student’s t-test was used to compare the efficacy of the associations of the two *** 1-min contact time,ALX eradicated the biofilms at all concentrations except for 0.007 8%and 0.015 6%–0.007 8%with *** ATCC 29212 and *** D1,*** results for eradication and concentration were obtained when it was combined with 100 mg？*** 5 min of contact time,ALX alone and combined with NAC eradicated all enterococci *** showed antimicrobial properties against the two *** strain biofilms tested at very low concentrations,and its combined use with NAC was not seen to enhance its activity.

关键词： alexidine antimicrobial activity biofilm Enterococcus faecalis N-acetylcysteine

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