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Occurrence of tetracycline residues and antimicrobial resistance in gram negative bacteria isolates from cattle farms in Spain

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Advances in Bioscience and Biotechnology 2013年第2期4卷 295-303页

作者： Matilde Carballo Fernando Esperón carlos sacristán Miguel González Belén Vázquez Sonia Aguayo Ana de la Torre national Centre for Environmental Health Group of Water Pollution Instituto de Salud Carlos III Madrid Spain Research Centre in Animal Health CISA-INIA Madrid Spain

The usage of antibiotics in animal husbandry has dramatically increased the concentration of antibiotic residues and has promoted the development and abundance of antibiotic resistance in manure. When it is spread onto agricultural land, both residues of antibiotics and bacteria carrying antibiotic resistance genes may be introduced into the environment. In this research, we isolated resistant gram negative bacteria from manure produced in two dairy and two beef cattle farms, located in Madrid (Spain), to determine their resistance to seventeen representative antibiotics commonly used in veterinary therapy. A total of 63 isolates were used to assess the overall bacterial antimicrobial resistance on cattle manure samples. Predominant species were Escherichia coli and Comamonas testosteroni accounting for 25% and 19.6% of the total, respectively. The most found antimicrobial resistances in gram-negative bacteria were to tetracycline (66.7%), sulphamethoxazole (55.6%), ampicillin (52.4%), cephalothin (46.0%), chloramphenicol (44.4%), nalidixic acid (39.7%) and trimethroprim- sulphamethoxazole (33.3%). The mean of resistance and the percentage of multi-resistant bacteria in beef farms were higher and statistically significant when compared to dairy farms which is opposite from the findings of the previous studies. The presence of three tetracyclines in all manure samples was also examined with stable recoveries (76% - 82%) and high sensitivity (limit of quantification 0.015 - 0.03 μg/kg). The concentrations of tetracyclines detected (nsistent to the theoretical tetracycline levels in manure in Spain according to the ex- cretion rate of these antibiotics and the values re-ported in scientific literature in other European coun- tries.

关键词： Antimicrobial Resistance Gram negative Bacteria Cattle Manure Tetracycline

Advancing healthcare through laboratory on a chip technology:Transforming microorganism identification and diagnostics

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World Journal of Clinical Cases 2025年第3期13卷 9-19页

作者： carlos M Ardila Department of Basic Sciences Biomedical Stomatology Research GroupUniversidad de Antioquia U de AMedellín 0057Colombia

In a recent case report in the World Journal of Clinical Cases,emphasized the crucial role of rapidly and accurately identifying pathogens to optimize patient treatment ***-on-a-chip(LOC)technology has emerged as a transformative tool in health care,offering rapid,sensitive,and specific identification of *** editorial provides a comprehensive overview of LOC technology,highlighting its principles,advantages,applications,challenges,and future *** studies from the field have demonstrated the practical benefits of LOC devices in clinical diagnostics,epidemiology,and food *** studies have underscored the superiority of LOC technology over traditional methods,showcasing improvements in speed,accuracy,and *** future integration of LOC with biosensors,artificial intelligence,and data analytics promises further innovation and *** call to action emphasizes the importance of continued research,investment,and adoption to realize the full potential of LOC technology in improving healthcare outcomes worldwide.

关键词： Laboratory-on-a-chip Microorganism identification Diagnostics Point-ofcare testing Biosensors

Efficacy of antimicrobials in preventing resistance in solid organ transplant recipients:A systematic review of clinical trials

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World Journal of Transplantation 2025年第1期15卷 156-164页

作者： carlos M Ardila Pradeep K Yadalam Jaime Ramírez-Arbelaez Department of Basic Sciences Faculty of Dentistry Biomedical Stomatology Research GroupUniversidad de Antioquia U de AMedellín 0057Colombia Department of Postdoctoral Program CIFE University CenterCuernavaca 62330MorelosMexico Department of Periodontics Saveetha Dental CollegeSaveetha Institute of Medical and HealthSIMATSSaveetha UniversityChennai 600077Tamil NaduIndia Department of Transplantation Hospital San Vicente FundaciónRionegro 054047Colombia

BACKGROUnD In the absence of effective antimicrobials,transplant surgery is not viable,and antirejection immunosuppressants cannot be administered,as resistant infections compromise the life-saving goal of organ *** To evaluate the efficacy of antimicrobials in preventing resistance in solid organ transplant *** A systematic review was conducted using a search methodology consistent with the preferred reporting items for systematic reviews and *** review included randomized clinical trials that evaluated the efficacy of antimicrobial agents(prophylactic or therapeutic)aimed at preventing antimicrobial *** search strategy involved analyzing multiple databases,including PubMed/MEDLInE,Web of Science,Embase,Scopus,and SciELO,as well as examining gray literature sources on Google Scholar.A comprehensive electronic database search was conducted from the databases’inception until May 2024,with no language *** After the final phase of the eligibility assessment,this systematic review ultimate-ly included 7 articles.A total of 2318 patients were *** most studied microorganisms were cytomegalovirus,although vancomycinresistant enterococci,Clostridioides difficile,and multidrug-resistant Enterobacterales were also *** antimicrobials used in the interventions were mainly maribavir,valganciclovir,gancic-lovir,and *** concern,all clinical trials showed significant proportions of resistant microorga-nisms after the interventions,with no statistically significant differences between the groups(mean resistance 13.47%vs 14.39%),except for two studies that demonstrated greater efficacy of maribavir and valganciclovir(mean resistance 22.2%vs 41.1%in the control group;Pn three clinical trials were 75,and there were 24 graft rejections in two *** All clinical trials reported significant proportions of antimicrobial-resistant microor

关键词： Antimicrobial resistance Antimicrobials Antivirals Solid organ transplant recipients Infections Microorganisms Cytomegalovirus

Factors affecting populations of the endemic Iberian hare(Lepus granatensis)after the first myxomatosis outbreaks in Central Spain

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Integrative Zoology 2023年第6期18卷 981-993页

作者： carlos LÁZARO JoséLuis GUZMÁn Fabián CASAS carlos SÁnCHEZ-GARCÍA Department of Research Fundación ArtemisanCiudad RealSpain Department of Zoology Faculty of SciencesUniversity of GranadaGranadaSpain

The Iberian hare(Lepus granatensis)is an endemic species distributed in Spain and *** outbreaks affecting this species were detected in 2018 in Central and Southern Spain,spreading *** to evaluate factors affecting the status of hare population after the arrival of myxomatosis,we conducted 108 nocturnal hare counts in Central Spain during two study periods(winter/spring and summer/autumn)in 54 different hunting grounds,covering 1071 km and observing 884 *** mean density in winter/spring was 7.66 hares/100 ha,(range 6.14-9.54/100 ha),while in summer/autumn,it was 3.4 hares/100 ha(range 2.6-4.4/100 ha).Densities of hares were not affected by the dominant habitat and the presence/absence of myxomatosis *** were more abundant at hunting grounds at a higher altitude and in those conducting targeted management,while detection of myxomatosis was related to lower altitude and higher levels of game management.A MaxEnt model used to generate a risk map for myxomatosis occurrence showed that the temperature annual range was the most important predictor,which suggests that environmental factors affecting myxomatosis vectors(mosquitoes,fleas,and ticks)could play a key role in disease *** myxomatosis in hares is becoming endemic,hare densities may be improved by game management and the monitoring and surveillance of this emerging *** surveillance programs could be the basis of effective collaborations between hunters,researchers,and environmental managers.

关键词： game management habitat Iberian hare MaxEnt monitoring myxomatosis

An unsupervised pattern recognition methodology based on factor analysis and a genetic-DBSCAn algorithm to infer operational conditions from strain measurements in structural applications

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Chinese Journal of Aeronautics 2021年第2期34卷 165-181页

作者： Juan carlos PERAFAn-LOPEZ Julian SIERRA-PEREZ Grupo de Investigacion en Ingenieria Aeroespacial Universidad Pontificia BolivarianaCircular 170-01Medellin 050031Colombia

Structural Health Monitoring(SHM) suggests the use of machine learning algorithms with the aim of understanding specific behaviors in a structural system. This work introduces a pattern recognition methodology for operational condition clustering in a structure sample using the well known Density Based Spatial Clustering of Applications with noise(DBSCAn) *** methodology was validated using a data set from an experiment with 32 Fiber Bragg Gratings bonded to an aluminum beam placed in cantilever and submitted to cyclic bending loads under 13 different operational conditions(pitch angles). Further, the computational cost and precision of the machine learning pipeline called FA + GA-DBSCAn(which employs a combination of machine learning techniques including factor analysis for dimensionality reduction and a genetic algorithm for the automatic selection of initial parameters of DBSCAn) was measured. The obtained results have shown a good performance, detecting 12 of 13 operational conditions, with an overall precision over 90%.

关键词： Clustering DBSCAn Factor analysis FBGs Pattern recognition Strain field

COMPLEX SYSTEMS In SPORTS:InTRODUCTIOn TO THE SPECIAL ISSUE

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Journal of Systems Science & Complexity 2013年第1期26卷 1-3页

作者： MERELO Juan Julin MORA Antonio M COTTA carlos Depto.ATC CITICUniversidad de Granada Depto.LCC Universidad de Malaga

1 Complex Systems in Sports Sport is one of the most important activities of *** relevance goes beyond the physical/psychological benefits that their practicing provides(an aspect that justifies in itself its importance in our current society).In fact,be it as actually playing some sport or just watching some games,it provides a huge means for socialization and *** to say,the economical viewpoint is also enormously relevant:events such as the Olympic Games。

关键词：复杂系统运动教育手段社会化

Classical Electromagnetic Field Theory in the Presence of Magnetic Sources

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Chinese Physics Letters 2003年第3期20卷 321-324页

作者： Li Kang Chen Wen-Jun carlos M. naón Department of Physics Hangzhou Teacher's College Hangzhou 310036 Department of Physics Zhejiang University Hangzhou 310027 Instituto de Física La Plata Departamento de Física Facultad de Ciencias Exactas Universidad Nacional de La Plata CC 67 1900 La Plata Argentina

Using two new well-defined four-dimensional potential vectors, we formulate the classical Maxwell field theory in a form which has manifest Lorentz covariance and SO(2) duality symmetry in the presence of magnetic sources. We set up a consistent Lagrangian for the theory. Then from the action principle we obtain both Maxwell's equation and the equation of motion of a dyon moving in the electromagnetic field.

关键词： Maxwell equations

Energy autonomous electronic skin

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npj Flexible Electronics 2019年第1期3卷 1-24页

作者： carlos García núñez Libu Manjakkal Ravinder Dahiya Bendable Electronics and Sensing Technologies(BEST)Group School of EngineeringUniversity of GlasgowG128QQ GlasgowUK Scottish Universities Physics Alliance(SUPA) Institute of Thin FilmsSensors&Imaging(TFSI)University of the West of ScotlandPaisley PA12BEUK

Energy autonomy is key to the next generation portable and wearable systems for several *** these,the electronic-skin or e-skin is currently a matter of intensive investigations due to its wider applicability in areas,ranging from robotics to digital health,fashion and internet of things(IoT).The high density of multiple types of electronic components(***,actuators,electronics,etc.)required in e-skin,and the need to power them without adding heavy batteries,have fuelled the development of compact flexible energy systems to realize self-powered or energy-autonomous *** compact and wearable energy systems consisting of energy harvesters,energy storage devices,low-power electronics and efficient/wireless power transfer-based technologies,are expected to revolutionize the market for wearable systems and in particular for *** paper reviews the development in the field of self-powered e-skin,particularly focussing on the available energy-harvesting technologies,high capacity energy storage devices,and high efficiency power transmission *** paper highlights the key challenges,critical design strategies,and most promising materials for the development of an energy-autonomous e-skin for robotics,prosthetics and wearable *** paper will complement other reviews on e-skin,which have focussed on the type of sensors and electronics components.

关键词： electronic autonomous harvesting

A nETWORK AnALYSIS OF THE 2010 FIFA WORLD CUP CHAMPIOn TEAM PLAY

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Journal of Systems Science & Complexity 2013年第1期26卷 21-42页

作者： COTTA carlos MORA Antonio M MERELO Juan Julin MERELO-MOLInA Cecilia Department of Computer Science University of Mlaga Department of Architecture and Computer Technology University of Granada

This paper analyzes the network of passes among the players of the Spanish team during the last FIFA World Cup 2010,where they emerged as the champion,with the objective of explaining the results obtained from the behavior at the complex network *** team is considered a network with players as nodes and passes as(directed) edges.A temporal analysis of the resulting passes network is also done,looking at the number of passes,length of the chain of passes,and to network measures such as player centrality and clustering *** of the last three matches(the decisive ones) indicate that the clustering coefficient of the pass network remains high,indicating the elaborate style of the Spanish *** effectiveness of the opposing team in negating the Spanish game is reflected in the change of several network measures over time,most importantly in drops of the clustering coefficient and passing length/speed,as well as in their being able in removing the most talented players from the central positions of the ***'s ability to restore their combinative game and move the focus of the game to offensive positions and talented players is shown to tilt the balance in favor of the Spanish team.

关键词： Complex network complex systems FIFA World Cup football La Roja network analysis soccer Spanish team sports.