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Single Incision Laparoscopic Surgery Development at the Hospital Clinic: Our 4-Year Experience

International Journal of Clinical Medicine 2015年第1期6卷 26-32页

作者： Josep Martí Oscar Vidal Mauro Valentini Cesar Ginestà Juan José Espert Miss Yolanda rodriguez Guerson Benarroch Juan carlos García-Valdecasas General Surgery and Emergency Unit Department of General and Digestive Surgery Digestive Diseases and Metabolism Institute Hospital Clínici Provincial Universitat de Barcelona IDIBAPS Barcelona Spain

Background: Single incision laparoscopic surgery (SILS) has still not replaced conventional laparoscopic surgery in many centers because of its special instruments requirements and training needs. In our Institution we introduced SILS procedures in a procedural stepwise manner, with progression to more difficult procedures only allowed if complete proficiency in low-complexity ones is achieved. Methods: We studied the evolution of performed SILS procedures in our Institution from the beginning of the program (mainly appendectomies, cholecystectomies, left adrenalectomies) and the results from these techniques. Results: Among 328 SILS procedures there were 148 appendectomies (24 by suprapubic access), 141 cholecystectomies, 32 left adrenalectomies, 6 diagnostic laparoscopies and 1 resection of Meckel’s diverticulum. The majority of appendectomies accounted for non-perforated appendicitis cases and there were no significant differences between transumbilical and suprapubic access groups. In cholecystectomies the majority of patients were elective cases and oral intake could be resumed within the 6 postoperative hours. In left adrenalectomies no conversion to open surgery was required and there were no intraoperative or postoperative complications. Conclusions: Procedural stepwise approach in SILS is a valid, feasible and safe option that can be applied at any institution starting a SILS program.

关键词： Adrenal Appendix Cholecystectomy Training/Courses Education

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西班牙在华FDI进程与趋势分析

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经济与管理评论 2012年第4期28卷 62-66,132页

作者： Yu Hu Gonzalo Maroto Esteban carlos rodriguez Monroy 马德里理工大学西班牙马德里通用电气医疗集团西班牙马德里

目前,中国已成为世界上第二大FDI接受国。西班牙也是世界上对外投资最多的国家之一。一直以来,西班牙的传统投资目标是北非和南美。2007年以前,西班牙在中国的投资很少,但2007年和2010年投资量显著增长,并显示出长期持续增长的趋势。分... 详细信息

目前,中国已成为世界上第二大FDI接受国。西班牙也是世界上对外投资最多的国家之一。一直以来,西班牙的传统投资目标是北非和南美。2007年以前,西班牙在中国的投资很少,但2007年和2010年投资量显著增长,并显示出长期持续增长的趋势。分析中国发展历程中FDI骤增所带来的影响,以及中国与西班牙两国经济事务等关系的发展,并运用SWOT分析法对西班牙在华FDI进行探究,中国无疑是西班牙在亚洲最主要的投资目标国,西班牙为加深两国经济关系做出了不懈的努力。然而,与中国所具备的潜力相比,投资仍然偏低,在不久的将来,随着服务贸易自由化进程的加快,新的机遇会越来越多。

关键词：西班牙在华FDI 进程与趋势 SWOT分析

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ASE Noise Characterization of an All-Fiber Sagnac Interferometer via LAN for Remote Sensing

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Open Journal of Applied Sciences 2018年第12期8卷 554-575页

作者： Abraham Sierra-Calderon Jose carlos rodriguez-Novelo Manuel May-Alarcon Jaime Rafael Ek-Ek Jose Alfredo Alvarez-Chavez Herman Leonard Offerhaus Centro de Investigacion e Innovacion Tecnologica Instituto Politecnico Nacional Mexico City Mexico Facultad de Ingenieria Universidad Autonoma del Carmen Ciudad del Carmen Mexico Optical Sciences Group University of Twente Enschede The Netherlands

The spectral noise characteristic and relative intensity noise of an all-fibre Sagnac interferometer consisting of pump source, a WDM, a piece of Er-doped fibre, a fibre Bragg grating (FBG), an optical circulator and a 50/50 coupler, were studied over a 75C-degree range. At the probing end, a high-birefringence piece of fibre and a Peltier were employed for temperature variation. Spectral and temperature response of the noise reduction due to temperature variation was performed remotely using an Arduino micro-controller and a DS18B20 digital sensor and fed into a local area network. Optical and thermal charac-terization of the system has also been undertaken.

关键词： Amplified Spontaneous Emission Noise Sagnac Interferometer Optical Fibre Sensor Erbium-Doped Fibre Amplifier Fibre Bragg Grating

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Study and ICH validation of a reverse-phase liquid chromatographic method for the quantification of the intact monoclonal antibody cetuximab

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Journal of Pharmaceutical Analysis 2016年第2期6卷 117-124页

作者： Antonio Martinez-Ortega Agustin Herrera Antonio Salmeron-Garcia Jose Cabeza Luis Cuadros-rodriguez Natalia Navas Department of Analytical Chemistry University of Granada Faculty of Science Campus Fuentenueva s/n E-18071 Granada Spain UGC intercentro Interniveles Farmacia Granada San Cecilio Hospital Biomedical Research Institute ibs. GRANADA. Hospitales Universitarios de Granada University of Granada E-18012 Granada Spain Department of Analytical Chemistry Science Faculty Biomedical Research Institute ibis. GRANADA University of Granada Campus Fuentenuevo s/n E-18071 Granada Spain

Cetuximab （CTX） is a potent chimeric mouse/human monoclonal antibody （mAb） approved worldwide for treatment of metastatic colorectal cancer. Among the various biological and physical analyses per- formed for full study on this biopharmaceutic, the determination of the concentration preparations throughout manufacturing and subsequent handling in hospital is particularly relevant. In the present work, the study and validation of a method for quantifying intact CTX by reverse-phase high-perfor- mance liquid chromatography with diode array detection （（RP）HPLC/DAD） is presented. With that end, we checked the performance of a chromatographic method for quantifying CTX and conducted a study to validate the method as stability-indicating in accordance with the International Conference on Harmo- nization guidelines （ICH） for biotechnological drugs; therefore, we evaluated linearity, accuracy, preci- sion, detection and quantification limits, robustness and system suitability. The specificity of the method and the robustness of the mAb formulation against external stress factors were estimated by compre- hensive chromatographic analysis by subjecting CTX to several informative stress conditions. As de- monstrated, the method is rapid, accurate, and reproducible for CTX quantification. It was also suc- cessfully used to quantify CTX in a long-term stability study performed under hospital conditions.

关键词： Reverse-phase high performance liquidchromatography Diode detector Stress study Biopharmaceuticals Cetuximab

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Inclusion Levels of Fermented Apple Bagasse on in Vitro Rumen Fermentation of Alfalfa Hay

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Journal of Agricultural Science and Technology(A) 2015年第1期5卷 40-46页

作者： Yamicela Castillo-Castillo Oscar Ruiz-Barrera Eduviges Burrola-Barraza Claudio Arzola-Alvarez Agustin Corral-Luna carlos rodriguez-Muela Manuel Murillo-Ortiz Department of Veterinary Medicine Multidisciplinary Division Autonomous University of Juarez City Nuevo Casas GrandesChihuahua 31803 Mexico College of Animal Science and Ecology Autonomous University of Chihuahua Chihuahua 31453 Mexico Faculty of Veterinary Medicine and Animal Science Juarez University of the State of Durango Durango 34620 Mexico

The aim of this study was to assess the effect of inclusion of fermented apple bagasse （FAB） obtained through solid state fermentation on pH, ammonia nitrogen （N-NH3）, volatile fatty acids （VFA） content, in vitro dry matter digestibility （IVDMD）, lactic acid and microbial counting of alfalfa hay under in vitro rumen environment; four levels of FAB were evaluated （0, 0.25, 0.50 and 0. 75 g/dry matter of FAB） replacing 1.5 g dry matter （DM） of alfalfa hay and incubated at different fermentation times （0, 4, 8, 12 and 24 h） using a complete random design with repeated measures on time. Counts of live yeast colonies （6.08, 6.33, 6.24 and 6.51 CFU/mL expressed as log 10） was higher when FAB was included in the different levels up to the 12 h of fermentation （P 〈 0.0001）; lactic acid content also increased as FAB was included in the different levels （10.61, 13.86, 16.84 and 14.57μg/mL） up to the 12 h of incubation （P 〈 0.001）. Nevertheless, the other variables measured as pH, N-NH3, VFA, IVDMD, total bacteria and fungi counts, were not affected by the treatments. It is concluded that substitution of FAB by alfalfa hay in an in vitro rumen ecosystem positively modified live yeast colonies and lactic acid concentration, without effect on the other fermentative and microbial parameters of the in vitro rumen environment, but considering mixes of FAB and alfalfa hay as a quality ingredient for the feeding of ruminants.

关键词： Apple bagasse alfalfa microbial solid state fermentation.

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High efficiency gas-liquid separation system for pumped wells

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Petroleum 2019年第2期5卷 178-182页

作者： L.Enrique Ortiz-Vidal Marcel C.Barbosa Oscar M.H.rodriguez Industrial Multiphase Flow Laboratory(LEMI) Mechanical Engineering DepartmentS~ao Carlos School of EngineeringUniversity of S~ao Paulo(USP)13563-120Sao CarlosSPBrazil

Gas-liquid separation is a very common process in industrial plants,where often high Efficiency of Gas Separation(EGS)is *** the upstream oil industry,gas separation is also crucial for the proper operation of Electrical Submergible Pumps(ESP).Recent studies report the excellent performance of a gravitational separator known as Inverted-Shroud separator(IS-separator).Laboratory results indicate that this kind of separator can achieve total gas separation for a wide range of operation conditions under continuous two-phase *** original intent is to use the IS-separator for downhole gas separation in oil production ***,by account of its simple design and relatively compact size,it may be suitable for using in industrial *** this paper,we present the IS-separator in details,including geometry characteristics and *** equipment performance is also *** addition,the challenges related to the deep understanding of the gas separation process inside the IS-separator and the possible practical solutions are outlined.

关键词： separation pumped details

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Bioactive Amines: Aspects of Quality and Safety in Food

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Food and Nutrition Sciences 2014年第2期5卷 138-146页

作者： Mariana Bacellar Ribas rodriguez Carla da Silva Carneiro Marcia Barreto da Silva Feijó carlos Adam Conte Júnior Sérgio Borges Mano Department of Food Science Federal Fluminense University Niterói Brazil. Department of Food Technology Federal Fluminense University Niterói Brazil Department of Natural Products and Food Federal University of Rio de Janeiro Rio de Janeiro Brazil

Presented in several types of food, bioactive amines are described as organic bases of low molecular weight. They have vasoactive, psychoactive and toxicological characteristics and constitute a potential health risk. The concentration of amines formed in foods depends on the type of microorganisms present, the action of decarboxylase enzymes produced by microorganisms on specific amino acids and favorable conditions for enzymatic activity. The presence of these chemical metabolites has been suggested as a quality indicator in routine analyzes for food production and marketing monitoring. The detection of bioactive amines can be performed by chromatographic methods, fluorometric and enzymatic kits. Bioactive amine formation can be prevented mainly through the adoption of good manufacturing practices, but the industry can also use other methods such as temperature control in the production chain, modified atmosphere packaging and food irradiation. This review aims to address the formation of bioactive amines in foods, emphasizing the formation and classification of these metabolites, aspects related to health, acceptable limits, detection methods and control methods used in the industry to ensure food safety and quality. The success of this approach is linked to the importance of bioactive amines as quality indicators, as well as the discussion on the development of methodologies for determining these substances and discussion of acceptable parameters in food.

关键词： Bioactive Amines Detection Methods Toxicity Acceptable Limits Food Quality

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αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model

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Protein & Cell 2020年第3期11卷 219-226页

作者： Paloma Martinez-Redondo Isabel Guillen-Guillen Noah Davidsohn Chao Wang Javier Prieto Masakazu Kurita Fumiyuki Hatanaka Cuiqing Zhong Reyna Hernandez-Benitez Tomoaki Hishida Takashi Lezaki Akihisa Sakamoto Amy NNemeth Yuriko Hishida Concepcion rodriguez Esteban Kensaku Shojima Ling Huang Maxim Shokhirev Estrella Nunez-Delicado Josep MCampistol Isabel Guillen-Vicente Elena rodriguez-Inigo Juan Manuel Lopez-Alcorocho Marta Guillen-Vicente George Church Pradeep Reddy Pedro Guillen-Garcia Guang-Hui Liu Juan carlos Izpisua Belmonte Gene Expression Laboratory Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Wyss Institute for Biologically Inspired Engineering Harvard UniversityCambridgeMA 02115USA Department of Genetics Harvard Medical SchoolBostonMA 02115USA Integrative Genomics and Bioinformatics Core Salk Institute for Biological Studies10010 North Torrey Pines RoadLa JollaCA 92037USA Universidad Catolica San Antonio de Murcia(UCAM) Campus de los JeronimosNo 1351230107 GuadalupeSpain Hospital Clinic of Barcelona Career Villarroel17008036 BarcelonaSpain Department of Traumatology and Research Unit Clinica CEMTRO28035 MadridSpain National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Institute for Stem cell and Regeneration Chinese Academy of SciencesBeijing 100101China Translational Medical Center for Stem Cell Therapy Shanghai East HospitalTongji University School of MedicineShanghai 200120China

Dear Editor,Homeostasis and repair are critical biological processes that allow for tissue and organ preservation and function in multicellular *** regulation and extension vary drastically across the animal kingdom,and mammals show limited tissue-specific regenerative capacity that declines with *** aging,articular cartilage is one of the tissues that undergo substantial changes in the matrix structure,molecular composition,metabolic activity,and mechanical properties(Loeser et al.2016).

关键词： aging treatment critical

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ISCHEMIA trial:How to apply the results to clinical practice

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World Journal of Cardiology 2021年第8期13卷 237-242页

作者： Rafael Vidal-Perez Alberto Bouzas-Mosquera Jesus Peteiro Jose Manuel Vazquez-rodriguez Servicio de Cardiologia Unidad de Imagen y Funcion CardiacaComplexo Hospitalario Universitario A Coruna(CHUAC)Centro de Investigacion Biomedica en Red(CIBERCV)-Instituto de Salud Carlos IIIA Coruna 15006Spain Servicio de Cardiología Complexo Hospitalario Universitario A CoruñaA Coruña 15006Spain

During the last years two questions have been continuously asked in chronic coronary syndromes:(1)Do revascularization procedures(coronary artery bypass grafting or percutaneous coronary intervention)really improve symptoms of angina?and(2)Do these techniques improve outcomes,*** they prevent new myocardial infarction events and cardiovascular death?Therefore,there was a need for a large definitive *** study was the ISCHEMIA trial,a large,multicentric trial sponsored by the National Heart,Lung,and Blood *** main trial compared coronary revascularization and optimal medical treatment(OMT)vs OMT alone in 5179 patients enrolled after a stress *** a median 3.2-year follow-up,318 primary outcome events occurred;the adjusted hazard ratio for the invasive strategy as compared with the conservative strategy was 0.93(95%confidence interval 0.80-1.08,P=0.34).The ISCHEMIA trial deeply disrupted many of our prior attitudes regarding management strategies for patients with stable coronary artery *** findings underscore the benefits of disease-modifying OMT for stable coronary artery disease *** main purposes of ischemia assessment before this trial were:Diagnostic purposes,assessment of outcome,and adding to decision-making ***,this changed after the trial *** results of ISCHEMIA might challenge the current diagnostic approach for stable angina patients recommended in the last European Society of Cardiology guidelines on chronic coronary disease that were based on studies published before the ISCHEMIA *** this editorial we propose our approach based on the ISCHEMIA study and the pretest probability for a positive test in patients with chronic coronary syndromes.

关键词： Stable angina Chronic coronary syndrome ISCHEMIA Stress testing Therapy Diagnosis

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Mutations in foregut SOX2^+ cells induce efficient proliferation via CXCR2 pathway

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Protein & Cell 2019年第7期10卷 485-495页

作者： Tomoaki Hishida Eric Vazquez-Ferrer Yuriko Hishida-Nozaki Ignacio Sancho-Martinez Yuta Takahashi Fumiyuki Hatanaka Jun Wu Alejandro Ocampo Pradeep Reddy Min-Zu Wu Laurie Gerken Reuben J. Shaw Concepcion rodriguez Esteban Christopher Benner Hiroshi Nakagawa Pedro Guillen Garcia Estrella Nunez Delicado Antoni Castells Josep M. Campistol Guang-Hui Liu Juan carlos Izpisua Belmonte Gene Expression Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Advaneed Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric Disorders Xuanwu Hospital Capital Medical University Beijing 100053 China National Laboratory of Biomacromolecules CAS Center for Excellence in Biomacromolecules Institute of Biophysics Chinese Academy of Sciences Beijing 100101 China University of the Chinese Academy of Sciences Beijing 100049 China Insitute for Stem Cell and Regeneration Chinese Academy of Sciences Beijing 100101 China Beijing Institute for Brain Disorder Beijing 100069 China Universidad Catolica San Antonio de Murcia Campus de los Jeronimos 135 Guadalupe 30107 Spain Molecular and Cell Biology Laboratory Dulbecco Center for Cancer Research Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Howard Hughes Medical Institute Dulbecco Center for Cancer Research Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Integrative Genomics Core Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA Division of Gastroenterology Department of Medicine Perelman School of Medicine University of Pennsylvania Philadelphia PA 19104 USA Abramson Cancer Center University of Pennsylvania Philadelphia PA 19104 USA Department of Traumatology and Research Unit Clinica CEMTRO Av. Ventisquero de la Condesa 42 Madrid 28035 Spain Gastroenterology Department Hospital Clinic University of Barcelona IDIBAPS CIBEREHD Barcelona 08036 Spain

Identification of the precise molecular pathways involved in oncogene-induced transformation may help us gain a better understanding of tumor initiation and promotion. Here, we demonstrate that SOX2^+ foregut epithelial cells are prone to oncogenic transformation upon mutagenic insults, such as Kras^G12D and p53 deletion. GFP-based lineage-tracing experiments indicate that SOX2^+ cells are the cells-of-origin of esophagus and stomach hyperplasia. Our observations indicate distinct roles for oncogenic KRAS mutation and P53 deletion. p53 homozygous deletion is required for the acquisition of an invasive potential, and Kras^G12D expression, but not p53 deletion, suffices for tumor formation. Global gene expression analysis reveals secreting factors upregulated in the hyperplasia induced by oncogenic KRAS and highlights a crucial role for the CXCR2 pathway in driving hyperplasia. Collectively, the array of genetic models presented here demonstrate that stratified epithelial cells are susceptible to oncogenic insults, which may lead to a better understanding of tumor initiation and aid in the design of new cancer therapeutics.

关键词： Sox2 tumor CXCR2 stratified epithelia

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