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Antimicrobial Activity, Structural, Crystallographic and Thermal Characteristics of Alpha-Titanium Phosphate Promoted by Silver Ions

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Materials Sciences and Applications 2024年第8期15卷 253-269页

作者： Enzo Erbisti Garcia Agnes Maria Cupertino Fernandes Araujo Gerson Alberto Valencia Albitres Daniela de França da Silva Freitas Danielle Mattos Mariano carlos magno fialho soares Sibele Piedade Cestari Marco Antônio Lemos Miguel Luis Claudio Mendes Institute of Macromolecules Professora Eloisa Mano Federal University of Rio de Janeiro Avenida Horacio Macedo 2030 Centro de Tecnologia Bloco J Ilha do Fundo Rio de Janeiro Brazil Institute of Microbiology Paulo de Ges Federal University of Rio de Janeiro Centro de Ciencias da Saude Bloco I Ilha do Fundo Rio de Janeiro Brazil Innovation in Polymer Engineering (PIEP) University of Minho Guimares Portugal

The recent global spread of the pandemic underscores the necessity of seeking new materials effective against microorganisms. Nanotechnology offers avenues for developing multifunctional materials. In this study, alpha-titanium phosphate (α-TiP) nanoparticles were synthesized and treated with silver salt to enhance their antimicrobial properties. The physicochemical characteristics and antimicrobial activity were evaluated. It was revealed by X-ray diffraction analysis that the structural integrity of α-TiP was influenced by ethylenediamine and silver ions. Distinct degradation profiles for each chemical modification were shown by thermogravimetric analysis. Infrared spectroscopy detected shifts and new absorption peaks in the spectra depending on the type of modification. Energy dispersive spectroscopy confirmed the disaggregation of α-TiP galleries following the addition of silver salt, which increased their effectiveness against microorganisms. Notably, only the sample treated with silver ions exhibited antimicrobial action. Antimicrobial activity was tested against the bacteria of medical importance Escherichia coli, Salmonella Enteritidis, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus, Listeria momocytogenes and the yeast Candida albicans. All microorganisms were inhibited by sample containing silver. Minor inhibition was observed against the Gram-positive bacteria L. monocytogenes and Bacillus cereus, while the greatest inhibition occurred against the fungus (yeast) C. albicans. The results revealed a potential application of the nanoparticles for control of microorganisms in public health.

关键词： Titanium Phosphate Silver Nitrate Antimicrobial Action

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Advances in cellular technology in the hematology field: What have we learned so far?

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World Journal of Stem Cells 2015年第1期7卷 106-115页

作者： Gustavo Torres de Souza Claudineia Pereira Maranduba Camila Maurmann de Souza Danielle Luciana Aurora soares do Amaral Francisco carlos da Guia Rafaella de Souza Salomao Zanette Joao Vitor Paes Rettore Natana Chaves Rabelo Lucas Mendes Nascimento Icaro Franca Navarro Pinto Julia Boechat Farani Abrahao Elias Hallack Neto Fernando de Sa Silva carlos magno da Costa Maranduba Angelo Atalla Laboratory of Human Genetics and Cell Therapy Biology Department Biological Sciences Institute Federal University of Juiz de Fora Juiz de Fora 36036-900Brazil Haematology and Bone Marrow Transplantation Service Medical Clinic Department University Hospital Federal University of Juiz de Fora Juiz de Fora 36036-900 Brazil

Despite the advances in the hematology field, blood transfusion-related iatrogenesis is still a major issue to be considered during such procedures due to blood antigenic incompatibility. This places pluripotent stem cells as a possible ally in the production of more suitable blood products. The present review article aims to provide a comprehensive summary of the state-of-theart concerning the differentiation of both embryonic stem cells and induced pluripotent stem cells to hematopoietic cell lines. Here, we review the most recently published protocols to achieve the production of blood cells for future application in hemotherapy, cancer therapy and basic research.

关键词： Hematopoietic stem cells Progenitor Induced pluripotent stem cells Reprogramming Embryonic stem cells

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Rapid Differentiation of Closely Related Citrus Genotypes by Fluorescence Spectroscopy

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Advances in Bioscience and Biotechnology 2014年第11期5卷 903-914页

作者： Dayse Drielly Souza Santana-Vieira Debora Marcondes Bastos Pereira Milori Paulino Ribeiro Villas Boas Marina Franca e Silva magno Guimaraes Santos Fernanda Amato Gaiotto Walter dos Santos soares Filho Abelmon da Silva Gesteira Embrapa Cassava and Fruits Cruz das AlmasBrazil Department of Biological Sciences State University of Santa CruzIlheusBrazil Embrapa Instrumentation Sao CarlosBrazil

The differentiation of closely related Citrus genotypes is a meticulous, laborious, and time-consuming task that involves the assessment of complex traits such as growth, tolerance to stress, photosynthetic efficiency, yield, and many others. Such a task is generally accomplished either by analyzing specific features of adult plants or by applying molecular markers to young trees. On one hand, only after plants start yielding can distinct genotypes be differentiated by comparing their fruit sizes, shapes, taste, and the number of seeds. On the other hand, molecular markers are expensive, and demand expertise and time for the analysis of a larger number of plants. For these reasons, the development of techniques that could assist in an early, quick and accurate differen-tiation of closely related Citrus varieties is of utmost importance. In this context, laser-induced fluorescence spectroscopy (LIFS) is a promising technique, since it is rapid, highly sensitive, and inexpensive. Previous studies showed that LIFS can differentiate a variety of sweet orange. However, this new study aimed to determine LIFS accuracy in the differentiation and grouping of very closely varieties of four Sunki mandarin selections: Comum, Florida, Tropical, and Maravilha. Furthermore we compared the results with ISSR and SSR molecular markers for the same varieties. LIFS technique distinguished the four selections with accuracy greater than 70%. Only with molecular markers was possible distinguishing clearly Tropical from Maravilha, but not Comum from Florida selections. In this way the results suggest that LIFS may be a sound tool for helping the identification of closely Citrus varieties.

关键词： Differentiation of Citrus Genotypes Sunki LIFS Molecular Markers PLSR

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Punica granatum Leaf Lipophilic Fraction Favours Mortality by Attenuating Inflammation in Mice with Polymicrobial Sepsis

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Journal of Pharmacy and Pharmacology 2019年第7期7卷 355-367页

作者： Nagila Caroline fialho Sousa Isabella Fernandes da Silva Figueiredo Mizael Calacio de Araujo Elaine Pereira de Andrade Thais Costa Alves Bruna Letícia Rosa da Silva Domingos magno Santos Pereira Saulo JoseFigueiredo Mendes Joao Francisco Silva Rodrigues Jaqueline Pessoa Pontes Simone Aparecida Teixeira AndreAlvares Marques Vale Marcus Vinicius Dias-Souza Lidio Goncalves Lima-Neto Vera Lucia dos Santos Ana Paula Azevedo-Santos Marcelo Nicolas Muscara Valerio Monteiro-Neto Andrea de Souza Monteiro Luis Claudio Nascimento da Silva Elizabeth soares Fernandes Programa de Pos-graduacao Universidade CEUMARua JosueMontellon°1RenascencaSao LuisMA 65075-120Brazil Departamento de Farmacologia Universidade de Sao PauloAv.Prof.Lineu Prestesn°1524Sala 326ButantanSao PauloSP 05508-900Brazil Programa de Pos-graduacao em Ciências em Saude Universidade Federal do MaranhaoAvenida dos Portuguesesn°1966BacangaSao LuisMA 65080-805Brazil Departamento de Microbiologia Universidade Federal de Minas GeraisAv.Antonio Carlosn°6627PampulhaBelo HorizonteMG 31270-901Brazil Departamento de Patologia Universidade Federal do MaranhaoAvenida dos Portuguesesn°1966BacangaSao LuísMA 65080-805Brazil

Punica granatum *** known antimicrobial and anti-inflammatory *** of different parts of this plant have been commonly used in the folk medicine to prevent and treat infections;and studies have supported the use of *** extracts for this ***,little is known of the effects of ***-derived fractions in severe infectious diseases such as ***,we investigated the actions of the hexane fraction(HF),rich in lipophilic compounds,obtained from the leaves of this plant,in a murine model of polymicrobial *** pre-treatment with HF increased mortality in septic *** same animals presented with lower levels of interleukin-6,nitric oxide and hydrogen peroxide in their peritoneal lavage samples than vehicle *** also increased polymorphonuclear cell accumulation in to the peritoneum of mice with ***-derived nitric oxide upon lipopolysaccharide stimuli was markedly reduced by incubation with ***,triterpenes,phytosterols,vitamin E and ascorbyl palmitate were detected in the *** results indicate that *** leaf lipophilic fractions may worsen sepsis *** effect may be associated with its bioactive compounds which act synergistically or not,increasing sepsis mortality in vivo.

关键词： P.granatum infection inflammation

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