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1.3 μm emitting SrF2：Nd^3＋ nanoparticles for high contrast in vivo imaging in the second biological window

Nano Research 2015年第2期8卷 649-665页

作者： Irene Villa Anna Vedda Irene Xochilt Cantarelli Marco Pedroni Fabio Piccinelli Marco Bettinelli Adolfo Speghini Marta Quintanilla Fiorenzo Vetrone Ueslen Rocha carlos jacinto Elisa Carrasco Francisco Sanz Rodrfguez Angeles Juarranz Blanca del Rosal Dirk H. Ortgies Patricia Haro Gonzalez Jose Garcia Sole Daniel Jaque Garcia Department of Materials Science University of Milano-Bicocca Via R. Cozzi 55 20125 Milano Italy Dipartimento di Biotecnologie Universita di Verona and INSTM UdR Verona Strada le Grazie 15 37134 Verona Italy Institut National de la Recherche Scientifique-Energie Materiaux et Telecommunications Universitd du Quebec Varennes QC PQ J3X 1S2 Canada Grupo de Fotonica e Fluidos Complexos Instituto de Fisica Universidade Federal de Alagoas 57072-970 Maceio Alagoas Brazil Departamento de Biologia Facultad de Ciencias Campus de Cantoblanco Universidad Autdnoma de Madrid Madrid 28049 Spain Fluorescence Imaging Group Departamento de Fisica de Maferiales Facultad de Ciencias Campus de Cantoblanco Universidad Aut6noma de Madrid Madrid 28049 Spain

Novel approaches for high contrast, deep tissue, in vivo fluorescence biomedical imaging are based on infrared-emitting nanoparticles working in the so-called second biological window （1,000-1,400 nm）. This allows for the acquisition of high resolution, deep tissue images due to the partial transparency of tissues in this particular spectral range. In addition, the optical excitation with low energy （infrared） photons also leads to a drastic reduction in the contribution of autofluorescence to the in vivo image. Nevertheless, as is demonstrated here, working solely in this biological window does not ensure a complete removal of autofluorescence as the specimens diet shows a remarkable infrared fluorescence that extends up to 1,100 nm. In this work, we show how the 1,340 nm emission band of Nd3. ions embedded in SrF2 nanoparticles can be used to produce autofluorescence free, high contrast in vivo fluorescence images. It is also dem- onstrated that the complete removal of the food-related infrared autofluorescence is imperative for the development of reliable biodistribution studies.

关键词： fluorescence imaging rare earth dopednanoparticles nanomedicine

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Characterization of two Achromobacter xylosoxidans isolates from patients with pertussis-like symptoms

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Asian Pacific Journal of Tropical Medicine 2015年第6期8卷 465-468页

作者： Fiorella Orellana-Peralta Michelle jacinto Maria J.Pons Cláudia Gomes carlos Bada Isabel Reyes Juana del Valle Mendoza Joaquim Ruiz Facultad de Ciencias de la Salud.Universidad Peruana de Ciencias Aplicadas-LiPC LimaPeru ISGlobal Barcelona Ctr.Int.Health Res.(CRESIB)Hospital Clinic-Universitat de Barcelona Hospital de Emergencias Pediatricas.Lima Peru Instituto de Investigacion Nutrirional LimaPeru

Objective:To characterize two Achromobaeter xylosoxidans recovered from 2 patients diagnosed with pertussis during a Bordetella pertussis surveillance ***:Nasopharyngeal swabs from 2 children under 1 year of age with clinical suspicion of pertussis were analyzed by culture and ***:Two Achromobaeter xylosoxidans A8,closely related to Bordetella *** recovered from 2 patients diagnosed of pertussis,both carrying the ptxA gene and IS418 the pertussis toxin encoding ***,antibiotic susceptibility was evaluated by disk-diffusion method and by ***:Although more detailed studies are needed,the present data highlight the possibility that Achromobaeter *** related Bordetella pertussis microorganisms and not covered under the vaccine umbrella,might also result in cases of whooping *** further surveillance is necessary to determine the extension and relevance of their pathogenic role in order to discriminate their real public health implication.

关键词： Achromobacter xylosoxidans Bordetella pertussis

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A procedure to assess the spatial variability in the importance of abiotic factors affecting distributions： the case of world freshwater fishes

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Current Zoology 2018年第5期64卷 549-557页

作者： Ana M. MANJARRES-HERNANDEZ Castor GUISANDE Emilio GARCiA-ROSELLO Patricia PELAYO-VILLAMIL jacinto GONZALEZ-DAcOSTA Juirgen HEINE Luis GONZALEZ VILAS carlos GRANADO-LORENClO Santiago R. DUQUE Jorge M. LOBO alnstituto Amazonico de Investigaciones-IMANI Universidad Nacional de Colombia Leticia A.A. 215 Colombia 910001 Facultad de Ciencias del Mar Universidad de Vigo Vigo 36310 Spain Departamento de Informatica Edificio Fundicien Universidad de Vigo Vigo 36310 Spain Grupo de Iciologia Universidad de Antioquia Medellin Colombia 050010 eDepartamento de Biologia Vegetal y Ecologia Facultad de Biologia Universidad de Sevilla Sevilla 41080 Spain Departamento de Biogeografia y Cambio Global Museo Nacional de Ciencias Naturales (CSIC) Calle de Jose Gutierrez Abascal 2 Madrid 28006 Spain

Understanding the factors shaping species＇ distributions is a key longstanding topic in ecology with unresolved issues. The aims were to test whether the relative contribution of abiotic factors that set the geographical range of freshwater fish species may vary spatially and/or may depend on the geographical extent that is being considered. The relative contribution of factors, to discriminate between the conditions prevailing in the area where the species is present and those existing in the considered extent, was estimated with the instability index included in the R pack- age SPEDInstabR. We used 3 different extent sizes： 1） each river basin where the species is present （local）; 2） all river basins where the species is present （regional）; and 3） the whole Earth （global）. We used a data set of 16,543 freshwater fish species with a total of 845,764 geographical records, together with bioclimatic and topographic variables. Factors associated with tempera- ture and altitude show the highest relative contribution to explain the distribution of freshwater fishes at the smaller considered extent. Altitude and a mix of factors associated with temperature and precipitation were more important when using the regional extent. Factors associated with precipitation show the highest contribution when using the global extent. There was also spatial variability in the importance of factors, both between species and within species and from region to region. Factors associated with precipitation show a clear latitudinal trend of decreasing in importance toward the equator.

关键词： anisotropic predictors environmental data selection geographical background non-stationary predictors

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Diversity Dimensions of Freshwater Fish Species around the World

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Journal of Geographic Information System 2021年第1期13卷 1-18页

作者： carlos Granado-Lorencio Cástor Guisande Patricia Pelayo-Villamil Ana Manjarrés-Hernández Emilio García-Roselló Juergen Heine Elisa Pérez-Costas Luis González-Vilas jacinto González-Dacosta Jorge M. Lobo Departamento de Biología Vegetal y Ecología Facultad de Biología Universidad de Sevilla Seville Spain Facultad de Ciencias del Mar Universidad de Vigo Campus Lagoas-Marcosende Vigo Spain Grupo de Ictiología Universidad de Antioquia Medellín Colombia Instituto Amazónico de Investigaciones (IMANI) Universidad Nacional de Colombia Leticia Colombia Department of Computer Science Universidad de Vigo Campus Lagoas-Marcosende Vigo Spain Departamento de Biogeografía y Cambio Global Museo Nacional de Ciencias Naturales (CSIC) Madrid Spain

The quality and coverage of the available taxonomical and geographical information and the recognition that diversity is multi-faceted are two main factors that hinder to understand the spatial and temporal variations of biodiversity. In this study, we aim to quantify the global distribution of five diversity components used to assess freshwater fish diversity in river basins around the world. The multidimensional character of these diversity components was estimated and the so obtained diversity dimensions mapped. This was done taking into account those well-surveyed basins discriminated by considering collector’s curves, and additionally by controlling for the effect of survey effort on all considered diversity components. A total of 1,472,109 occurrence records were analysed, corresponding to 17,292 species of freshwater fishes. Five diversity components were considered: functional richness, species richness, taxonomic diversity, and two rarity measures. Well-surveyed river basins were discriminated using accumulation curves. The effects of survey biases and knowledge gaps were minimized by determining the relationship of each component with the completeness values calculated for each river basin. The geographical pattern derived from raw data is skewed by the unequal knowledge available, and all diversity components were positively correlated with completeness values. The first dimension described the association between species richness and functional diversity. The second dimension represented rarity and taxonomic diversity. The congruence between species richness and functional diversity suggests that ecosystem functions increase with the number of fish species present in river basins and that a decrease in species richness may involve a loss of functionality. The levels of rarity and taxonomic diversity of many species-poor basins found in arid and cold regions suggest that the distinctiveness of their freshwater fishes is primarily a consequence of how isol

关键词： Diversity Components Functional Diversity Species Richness Rarity Taxonomic Diversity

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