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Cyclophilins A and B oppositely regulate renal tubular epithelial cell phenotype

Journal of Molecular Cell Biology 2020年第7期12卷 499-514页

作者： Eduard Sarró Mónica Durán Ana Rico Diana Bou-Teen Vanesa Fernández-Majada Anthony J.Croatt Karl A.Nath Maria Teresa Salcedo Justin H.Gundelach daniel Batlle Richard J.Bram Anna Meseguer Renal Physiopathology Group CIBBIM-NanomedicineVall d’Hebron Research Institute08035 BarcelonaSpain Cardiovascular Diseases Group Vall d’Hebron Research Institute08035 BarcelonaSpain Biomimetic Systems for Cell Engineering Laboratory Institute for Bioengineering of Catalonia(IBEC)The Barcelona Institute of Science and Technology(BIST)08028 BarcelonaSpain Division of Nephrology and Hypertension and Department of Medicine Mayo ClinicRochesterMN 55905USA Department of Pathology Hospital Universitari Vall d’Hebron08035 BarcelonaSpain Department of Pediatric and Adolescent Medicine College of MedicineMayo ClinicRochesterMN 55905USA Department of Biochemistry and Molecular Biology College of MedicineMayo ClinicRochesterMN 55905USA Division of Nephrology and Hypertension Northwestern University Feinberg School of MedicineChicagoIL 60611USA Department of Immunology College of MedicineMayo ClinicRochesterMN 55905USA Departament de Bioquímica i Biologia Molecular Unitat de Bioquímica de MedicinaUniversitat Autònoma de Barcelona08193 BellaterraSpain Red de Investigación Renal(REDINREN) Instituto de Salud Carlos III-FEDER28040 MadridSpain

Restoration of kidney tubular epithelium following sublethal injury sequentially involves partial epithelial–mesenchymal transition(pEMT),proliferation,and further redifferentiation into specialized tubule epithelial cells(TECs).Because the immunosuppressant cyclosporine-A produces pEMT in TECs and inhibits the peptidyl-prolyl isomerase(PPIase)activity of cyclophilin(Cyp)proteins,we hypothesized that cyclophilins could regulate TEC *** we demonstrate that in cultured TECs,CypA silencing triggers loss of epithelial features and enhances transforming growth factorβ(TGFβ)-induced EMT in association with upregulation of epithelial repressors Slug and *** pro-epithelial action of CypA relies on its PPIase *** contrast,CypB emerges as an epithelial repressor,because CypB silencing promotes epithelial differentiation,prevents TGFβ-induced EMT,and induces tubular structures in 3D *** addition,in the kidneys of CypB knockout mice subjected to unilateral ureteral obstruction,inflammatory and pro-fibrotic events were *** silencing/knockout leads to Slug,but not Snail,*** support of Slug expression depends on its endoplasmic reticulum location,where it interacts with calreticulin,a calcium-buffering chaperone related to Slug *** CypB silencing reduces ionomycin-induced calcium release and Slug upregulation,we suggest that Slug expression may rely on CypB modulation of calreticulin-dependent calcium *** conclusion,this work uncovers new roles for CypA and CypB in modulating TEC plasticity and identifies CypB as a druggable target potentially relevant in promoting kidney repair.

关键词： cyclophilins epithelial phenotype Slug TGFβ UUO fibrosis

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Calibration of SWAT2009 Using Crop Biomass, Evapotranspiration, and Deep Recharge: Calera Watershed in Zacatecas, Mexico Case Study

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Journal of Water Resource and Protection 2012年第7期4卷 439-450页

作者： Jose R. ávila-Carrasco Francisco Mojarro Dávila daniel N. Moriasi Prasanna H. Gowda carlos Bautista-Capetillo Francisco G. Echavarria-Cháirez Jurgen D. Garbrecht Jean L. Steiner Terry A. Howell Edward T. Kanemasu Alan J. Verser Kevin Wagner Jairo Hernandez Department of Civil Engineering Boise State University Boise USA Instituto Nacional de Investigaciones Forestales Agricolas y Pecuarias (INIFAP) Zacatecas Calera de V.R. Zac. Mexico Office of Global Programs University of Georgia Georgia USA Texas Water Resources Institute Texas A&M Institute of Renewable Natural Resources College Station USA Universidad Autónoma de Zacatecas Zacatecas México USDA-ARS-Conservation and Production Research Laboratory Bushland USA USDA-ARS-Grazinglands Research Laboratory El Reno USA

Groundwater is the main source of water in the semi-arid Calera watershed, located in the State of Zacatecas, Mexico. Due to increasing population, rapid industrial growth, and increased irrigation to meet growing food demand, groundwater extraction in the Calera watershed are exceeding recharge rates. Therefore, development and evaluation of alter-native water management strategies are needed for sustainable development of the region. The Soil and Water Assessment Tool (SWAT) model was selected for this purpose as it has been used to simulate a wide range of agricultural production, the extensive testing and application in diverse watersheds worldwide, and the potential for future linkage of this model to groundwater models. However, crucial flow data which are commonly used for calibrating hydrologic models are not available in this watershed. This paper describes a novel calibration methodology that uses biomass and water balance approach which has potential for calibration of hydrologic models in ungauged or data-scarce watersheds, which are prevalent in many parts of the world. Estimated long-term annual average actual evapotranspiration (AET), and deep aquifer recharge rates and plant biomass values based on the expert knowledge of researchers and managers in the watershed were used as targets for calibration. The model performance was assessed using the Nash-Sutcliffe effi-ciency coefficient (NSE), coefficient of determination (R2), and percent bias (PBIAS, %) statistics. On average, the calibrated SWAT model yielded annual Nash-Sutcliffe efficiency coefficient values of 0.95, 0.99, and 0.85 for AET, recharge, and biomass, respectively. The coefficient of determination, values for AET, recharge, and biomass were 0.95, 0.94, and 0.99 respectively. The percent bias values of ±2.21%, ±0.18%, and ±0.96% for AET, recharge, and biomass, respectively, indicated that the model reproduced the calibration target values of the three water budget variables within an acce

关键词： SWAT Calera Watershed Scenarios Recharge Evapotranspiration Runoff Erosion

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Composition of Cachacas Produced from Five Varieties of Sugarcane and the Correlation of the Presence of Dhurrin in the Cane with That of Ethyl Carbamate in the Product

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American Journal of Plant Sciences 2019年第2期10卷 339-350页

作者： Francielli D’carlos Cravo Wilder Douglas Santiago Allan da Silva Lunguinho Richard Bispo Barbosa Renan Elan da Silva Oliveira Gabriela Fontes Alvarenga Stephano daniel Santos Rafaella Hilda Zaniti Souza Ellen Cristina de Souza Katia Julia de Almeida Josefina Aparecida de Souza David Lee Nelson Maria das Gracas Cardoso Department of Food Science Federal University of LavrasC.P.LavrasMinas GeraisBrazil Department of Chemistry Federal University of LavrasC.P.LavrasMinas GeraisBrazil Pro-Reitoria de Pesquisa e Pos-Graduacao Federal University of Vales de Jequitinhonha and MucuriDiamantinaMGBrazil

The present work sought to characterize the cyanogenic glycoside dhurrin in five sugarcane varieties and to determine its possible relationship with the formation of ethyl carbamate in cachaca. For each variety, methanol, ethyl acetate and hexane extracts were prepared and submitted to thin layer chromatography. Chromatographic spots were revealed with iodine vapors. The physical and chemical parameters of the spirits produced from the five different varieties of sugar cane and by different production processes were within the legal limits. Although not exceeding the limit for ethyl carbamate, a significant variation in the final concentration of this compound was observed for each sample analyzed. The Rf values for the dhurrin standard and for the sugarcane variety SP 83-2847 were equal, similar to those for SP 80-3280 and CTC 11 and different from the Rf values for the RB 86-7515 and IAC 86-2480 samples.

关键词： Cachaca Ethyl Carbamate Quality Glycosides Cyanide Dhurrin

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Study of Effects of Photodynamic Therapy(PDT),in Scar-Induced Skin Wounds in Rats Wistar:The New Clinical Perspective for Ulcers

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Modern Research in Inflammation 2017年第1期6卷 1-8页

作者： Marcus Vinicius de Mello Pinto Aline Ronis Sampaio Reggiani Vilela Goncalves carlos Eduardo Tenorio da Veiga Lamara Laguardia Valente Rocha daniel Almeida da Costa Lina Claudia Pereira Lopes Rafael Batista Ferreira Pamela Mara Cainelli Valim Kellen Regina Rodrigues Chaveiro Elias Sobreira Sathler Richard Raphael Borges Tavares Vieira Isabela Nardoni Bernardes Medical School of the University of Buenos Aires Bueinos AiresArgentina The Cellurare Institute Center of Laser Treatment and Cell Therapy PetropolisBrazil Department of Cellular and Structural Biology of Federal University of Vicosa VicosaBrazil Clinical Hospital of Correas Rio de JaneiroBrazil The Medical School at the Health Sciences Institute of the University Center of Caratinga CaratingaBrazil The Medical School at the Center of Higher Education of Valenca ValencaBrazil Luiz Gioseffi Jannuzzi School Hospital of the Dom Andre Arcoverde Foundation ValencaBrazil

Photodynamic therapy today is becoming an important role in the healing of lacerated tissues, since it has therapeutic resources capable of accelerating this process. One treatment option is the clinical phototherapy, and Photodynamic Therapy (PDT) is being widely used. This study aims to evaluate the effect of PDT on the healing of skin wounds in rats. We used a sample of 39 male rats Wistar divided into three groups, a control, a PDT-treated green and red with the last PDT. After 24 hours before the surgical incisions, PDT was used in both groups for 6 minutes and was evaluated in histological level, the inflammatory reaction and the repair process. The results showed that the granulation tissue was more developed in the irradiated group than in the control group and the amount of chronic inflammatory cells (monocytes, macrophages, lymphocytes and plasma cells) predominated with green phototherapy. The epithelialization in the wound margins and scarring with better quality occurred with red PDT (640 nm), which the higher deposition was of collagen. However, phototherapy not collimated of 640 nm (red) resulted in better anti-inflammatory effects. Given these answers with phototherapy not collimated green (525 nm) and red (640 nm), we conclude that the use of these wavelengths is possible with benefits, mainly with red LED.

关键词： Healing Photodynamic Therapy(PDT) Light Emitting Diodes(LEDs) Ulcer

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M89酒店意大利米兰

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世界建筑导报 2018年第5期33卷 80-83页

作者： luca lazzerotti mauro mandelli silvia calzetti alessandro leanti andrea sforzin carlos noriega daniel gonzalez santiago trujillo erica mensi federico simonetti gianluca iannotta niccolò genesio andrea martiradonna(摄) piuarch

M89酒店对面，是Caproni工厂规则排布的厂房，有着别样的棚屋顶，散发着工业考古学般的魅力。新建的M89四星级酒店，位于via Mecenate大街89号。酒店正对着百年前一大型飞机制造厂厂房，经过Piuarch精心改造和扩建，厂房现在已成为古驰... 详细信息

M89酒店对面，是Caproni工厂规则排布的厂房，有着别样的棚屋顶，散发着工业考古学般的魅力。新建的M89四星级酒店，位于via Mecenate大街89号。酒店正对着百年前一大型飞机制造厂厂房，经过Piuarch精心改造和扩建，厂房现在已成为古驰新总部大楼。

关键词：四星级酒店意大利米兰飞机制造厂厂房考古学 via 屋顶

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暴力的果实

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商业周刊（中文版） 2012年第19期 118-119页

作者： daniel Grushkin

美国中央情报局1954年在危地马拉策动了一场政变，或者说颠覆该国政权更为合适。美国特工们在该国挑拨离间．散布右翼暴动谣言，甚至在危地马拉城投掷烟雾弹．并派39岁的危国前陆军上校卡洛斯·卡斯特罗·阿玛斯（carlos Castillo Arma... 详细信息

美国中央情报局1954年在危地马拉策动了一场政变，或者说颠覆该国政权更为合适。美国特工们在该国挑拨离间．散布右翼暴动谣言，甚至在危地马拉城投掷烟雾弹．并派39岁的危国前陆军上校卡洛斯·卡斯特罗·阿玛斯（carlos Castillo Armas）带领一支雇佣兵团入侵该国。

关键词：美国中央情报局果实暴力危地马拉 1954年卡斯特罗卡洛斯政权

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Biomechanical Analysis of the STS Movement and Impact of an Assistive Device on the Elderly and People with Displacement Difficulties

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Journal of Mechanics Engineering and Automation 2019年第4期9卷 111-119页

作者： Rodrigo Goncalves de Souza Maciel daniel Kioshi Kawasaki Cavalcanti carlos Eduardo Guedes Catunda Fernando Ribeiro da Silva Department of Mechanical Engineering Federal Center of Technological Education Celso Suckow da Fonseca(CEFET/RJ)Rio de JaneiroRJ20271-110Brazil

This study presents a biomechanical analysis of the performance of the STS(sit-to-stand)movement applied to elderly and people with difficulty of displacement comparing it with and without an assistive technology device.A biomechanical kinemetry method was used for the movement evaluation.A two-dimensional virtual human model was developed through segmented ergonomic video analysis and the data obtained were numerically simulated to measure the inertial forces and torques of the complete execution of the STS *** device allows a vertical elevation of 0 to 400 mm and an anterior slope of up to 25°.A prototype was used to compare the movement with and without the assistive *** a result,the torques in the lower limbs’joints and the vertical ground reaction forces were reduced by up to 60%and 23%,*** was a reduction of up to 37°in the maximum trunk flexion angle during the *** horizontal displacement of the center of mass was reduced by up to 70%.

关键词： STS biomechanics assistive technologies kinemetry method ergonomics video analysis human model posture

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Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort

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World Journal of Gastroenterology 2019年第4期25卷 433-446页

作者： Patricia Prieto Rafael I Jaén daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá Department of Metabolism and Physiopathology of Inflammatory Diseases Instituto de Investigaciones Biomédicas Alberto Sols (CSIC-UAM) Centro de Investigación Biomédica en Red de Enfermedades Cardiovasculares (Ciber-CV) Instituto de Salud Carlos III (ISCIII) Laboratorio de Imagen Médica Hospital Universitario Gregorio Mara?ón Laboratorio de Proteómica Cardiovascular Centro Nacional de Investigaciones Cardiovasculares (CNIC) Instituto de Investigación Sanitaria del Hospital Universitario la Paz(IdiPaz) Programa de Medicina Predictiva y Personalizada del Cáncer (PMPPC) Fundación Instituto de investigación en ciencias de la salud Germans Trias i PujolCtra Can Ruti

BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in *** To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic *** Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap *** Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PN

关键词： Colon Carcinoma Cyclooxygenase Prostaglandin Proteomics High resolution magic angle spinning

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Electrical Conductivity Behavior of the Aluminum Alloy 2024 during Artificial Aging

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材料科学与工程（中英文A版） 2020年第2期10卷 53-59页

作者： Eduardo Luis Schneider and Thomas Gabriel Rosauro Clarke daniel Diehl carlos Köhler Eduardo Luis Schneider Thomas Gabriel Rosauro Clarke Federal University of Rio Grande do Sul Porto Alegre 15.090Brazil

Precipitation-hardened aluminum alloys need to be heat treated to achieve the mechanical properties required for their *** production of these materials can be optimized to make them more attractive and competitive comparing to other materials such as composites that have a growing and large market share in aeronautics *** way to do this is by controlling the artificial aging of precipitation-hardenable aluminum alloys,such as the 2000 series Al-Cu *** alloys can be monitored in real time by analyzing their conductivity behavior inside the *** objective of this work is to evaluate the electrical conductivity behavior in real time of the 2024 alloy during the artificial aging at 190°*** this,analyses were made in order to assess the behavior of the microhardness curve by aging time and its microstructural characterization with thermal treatments in the times of 1 h to 9 h interrupted every 1 *** results of the electrical conductivity versus hardness curve showed a significant correlation and indicate that this measure has great potential to be used as a tool to control the thermal treatment of aging.

关键词： Fur-terminal method heat treatment age hardening precipitation aluminum alloys aluminum 2024

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Nanocellulose-based porous materials: Regulation and pathway to commercialization in regenerative medicine

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Bioactive Materials 2023年第11期29卷 151-176页

作者： Filipe V.Ferreira Alana G.Souza Rubina Ajdary Lucas P.de Souza Jo˜ao H.Lopes daniel S.Correa Gilberto Siqueira Hernane S.Barud Derval dos S.Rosa Luiz H.C.Mattoso Orlando J.Rojas Nanotechnology National Laboratory for Agriculture(LNNA) Embrapa Instrumentation-Rua XV de Novembro1452S˜ao CarlosSP13560-979Brazil Center for Engineering Modelingand Applied Social Sciences(CECS)Federal University of ABC(UFABC)Santo Andr´eBrazil Department of Bioproducts and Biosystems School of Chemical EngineeringAalto UniversityP.O.Box 16300AaltoEspooFIN-00076Finland College of Engineering and Physical Sciences Aston Institute of Materials ResearchAston UniversityBirminghamUK Department of Chemistry Division of Fundamental Sciences(IEF)Technological Institute of Aeronautics(ITA)S˜ao Jose dos CamposSPBrazil Laboratory for Cellulose&Wood Materials Empa-Swiss Federal Laboratories for Materials Science and TechnologyÜberlandstrasse 1298600DübendorfSwitzerland Biopolymers and Biomaterials Laboratory(BIOPOLMAT) University of Araraquara(UNIARA)Araraquar114801-340S˜ao PauloBrazil Bioproducts Institute Department of Chemical&Biological EngineeringDepartment of Chemistry andDepartment of Wood ScienceThe University of British Columbi12360 East MallVancouverBCV6T 1Z3Canada

We review the recent progress that have led to the development of porous materials based on cellulose nanostructures found in plants and other resources. In light of the properties that emerge from the chemistry, shape and structural control, we discuss some of the most promising uses of a plant-based material, nanocellulose, in regenerative medicine. Following a brief discussion about the fundamental aspects of self-assembly of nanocellulose precursors, we review the key strategies needed for material synthesis and to adjust the architecture of the materials (using three-dimensional printing, freeze-casted porous materials, and electrospinning) according to their uses in tissue engineering, artificial organs, controlled drug delivery and wound healing systems, among others. For this purpose, we map the structure-property-function relationships of nanocellulose-based porous materials and examine the course of actions that are required to translate innovation from the laboratory to industry. Such efforts require attention to regulatory aspects and market pull. Finally, the key challenges and opportunities in this nascent field are critically reviewed.

关键词： Cellulose nanofibrils Cellulose nanocrystals Regenerative medicine Biomaterial Sustainable materials Green nanomaterials

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