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热带农业科技 1983年第3期 65-68页

作者： R.Romano,M.S.Beruardes,A.M.G.De castro,K.H.Chee,黄雅志巴西橡胶局巴西橡胶局巴西橡胶局亚橡胶研究院

南美叶疫病是世界植胶国非常关心的橡胶树病害。一九八三年五月在我国首都北京召开的国际天然橡胶会议上,巴西代表卡斯特罗作了《南美叶疫病及其防治》的学术报告,比较全面地介绍近来对该病研究的进展,使我们对南美叶疫病有了更明确的... 详细信息

南美叶疫病是世界植胶国非常关心的橡胶树病害。一九八三年五月在我国首都北京召开的国际天然橡胶会议上,巴西代表卡斯特罗作了《南美叶疫病及其防治》的学术报告,比较全面地介绍近来对该病研究的进展,使我们对南美叶疫病有了更明确的认识。

关键词：植胶橡胶树园艺作物热雾机天然橡胶代森锰锌巴西橡胶南美疫病巴伊亚苯菌灵苯来特抗氧化剂冠接飞机喷雾脱叶磷

Preparation of Reference Material for Proficiency Test for Enumeration of Coliforms in Cheese Matrix

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Detection 2013年第1期1卷 7-12页

作者： Marcelo Luiz Lima Brandao Carla de Oliveira Rosas Silvia Maria Lopes Bricio Valeria de Mello Medeiros Juliana de castro Beltrao da Costa Rodrigo Rollin Pinheiro Paola Cardarelli-Leite Marcus Henrique Campino de La Cruz Armi Wanderley da Nobrega Laboratory of Products Department of MicrobiologyNational Institute of Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroBrazil Proficiency Testing Program of Products Subject to Health Surveillance National Institute of Quality Control in HealthOswaldo Cruz FoundationRio de JaneiroBrazil

It is widely accepted that quantitative reference materials (RM), are indispensable tools for verification of the precision and accuracy of analytical measurements. The RM can be used by food microbiology laboratories, as part of their quality assurance programmes, to achieve their quality control. In Brazil, Anvisa RDC No. 12/01 specifies the enumeration of coliforms as one of the parameters for evaluating cheese quality. The aim of this study was to produce a quantitative RM for proficiency testing (PT) for use in the testing of enumeration of coliforms in cheese matrixes. A sample of an ultra-filtered cheese with a coliforms count of <3.0 MPN/g and a total n? of viable aerobes of 1.2 × 103 CFU/g was used as the matrix to produce the RM. The ultra-filtered cheese matrix was distributed in flasks, contaminated with a specific concentration of an Escherichia coli strain and submitted to freeze-drying. Sucrose was used as the cryo-protector. The RM produced was considered sufficiently homogeneous and stable at ≤ ?70?C during the entire study period (348 days). The material was also considered sufficiently stable at 4?C for six days, but instable at 30?C and 35?C for the same period. At ?20?C the RM was sufficiently stable for 161 days. It was concluded that the material showed all the necessary requirements for a quality RM to be used as PT items and could be transported to the laboratories taking part in a PT at up to 4?C for up to 6 days, since the results indicated maintenance of the cell concentrations during this period. This is the first study to describe a methodology for producing RM containing coliforms in a cheese matrix.

关键词： Coliforms Cheese Reference Material Proficiency Test

Oral cancer in patients younger than 45 years of age. A comparative study of ＂young＂ versus ＂old＂ patients with squamous cell carcinoma of the oral cavity

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中国口腔颌面外科杂志 2008年第B5期6卷 141-141页

作者： Liuzzi J Velasquez Y castro R Gardie J Agudo E Head and Neck Department Hospital Oncologico PadreMachado Caracas Venezuela

Feasibility and safety of autologous bone marrow mononuclear cell transplantation in patients with advanced chronic liver disease

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World Journal of Gastroenterology 2007年第7期13卷 1067-1073页

作者： Andre castro lyra Milena Botelho Pereira Soares luiz Flavio Maia da Silva Marcos Fraga Fortes André Goyanna Pinheiro Silva Augusto César de Andrade Mota Sheilla A Oliveira Eduardo lorens Braga Wilson Andrade de Carvalho Bernd Genser Ricardo Ribeiro dos Santos luiz Guilherme Costa lyra Hos-pital Sao Rafael Salvador Bahia Brazil Centro de Pesquisas Goncalo Moniz Fundacao Oswaldo Cruz Salvador Bahia Brazil BGStats Consulting Graz Austria

AIM： To evaluate the safety and feasibility of bone marrow cell （BMC） transplantation in patients with chronic liver disease on the waiting list for liver transplantation. METHODS： Ten patients （eight males） with chronic liver disease were enrolled to receive infusion of autologous bone marrow-derived cells. Seven patients were classified as Child-Pugh B and three as Child-Pugh C. Baseline assessment included complete clinical and laboratory evaluation and abdominal MRI. Approximately 50 mL of bone marrow aspirate was prepared by centrifugation in a ficoll-hypaque gradient. At least of 100 millions of mononuclear-enriched BMCs were infused into the hepatic artery using the routine technique for arterial chemoembolization for liver tumors. Patients were followed up for adverse events up to 4 mo. RESULTS： The median age of the patients was 52 years （range 24-70 years）. All patients were discharged 48 h after BMC infusion. Two patients complained ofmild pain at the bone marrow needle puncture site. No other complications or specific side effects related to the procedure were observed. Bilirubin levels were lower at 1 （2.19 ± 0.9） and 4 mo （2.10 ± 1.0） after cell transplantation that baseline levels （238 ± 1.2）. Albumin levels 4 mo after BMC infusion （3.73 ± 0.5） were higher than baseline levels （3.47 ± 0.5）. International normalized ratio （INR） decreased from 1.48 （SD = 0.23） to 1.43 （SD = 0.23） one month after cell transplantation. CONCLUSION： BMC infusion into hepatic artery of patients with advanced chronic liver disease is safe and feasible. In addition, a decrease in mean serum bilirubin and INR levels and an increase in albumin levels are observed. Our data warrant further studies in order to evaluate the effect of BMC transplantation in patients with advanced chronic liver disease.

关键词： Bone marrow Cell transplantation Liverfailure Stem cell Cirrhosis

古巴东部Moa-Baracoa铬铁矿记录多期次成矿作用

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古巴东部Moa-Baracoa铬铁矿记录多期次成矿作用

试验室研究一种新型透水性混凝土路面砖的块型设计过程

首届全国矿产勘查大会

作者：芮会超杨经绥连东洋吴魏伟刘飞 Angélica I.Llanes castro 邱添地球科学学院/中国地质大学(武汉) 地球科学与工程学院/南京大学自然资源部深地动力学实验室/中国地质科学院地质研究所

＜正＞铬是航天和军工特种钢所需的不可或缺的关键金属原料,属国家战略性关键金属,我国95%以上需求依赖进口。铬铁矿是自然界中唯一可用于提炼金属铬的矿物原料,目前世界上铬铁矿分为层状铬铁矿和豆荚状铬铁矿两类。豆荚状铬铁矿主要产...

＜正＞铬是航天和军工特种钢所需的不可或缺的关键金属原料,属国家战略性关键金属,我国95%以上需求依赖进口。铬铁矿是自然界中唯一可用于提炼金属铬的矿物原料,目前世界上铬铁矿分为层状铬铁矿和豆荚状铬铁矿两类。豆荚状铬铁矿主要产在蛇绿岩和造山带的地幔橄榄岩中,我国目前只有这一类型。豆荚状铬铁矿的产出特征和成因已有百年研究历史,虽然提出了岩浆熔离、地幔熔融残余和熔体/岩石反应等多种成因模式[1-3],但对于解释铬铁矿的复杂成分、结构和铬元素的富集成矿过程还存在巨大分歧,尤其,近年来蛇绿岩铬铁矿中发现金刚石等超高压-超还原矿物[4-8],使得对铬铁矿成因的认识又增添了新的制约和思考。

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建筑砌块与砌块建筑 2008年第4期 36-39,48页

作者： Daniel castro J.R.Bayon Jorge Rodriguez Nelson Gonzalez-Angullo 陈义荣（译）杜建东（校）刘黎（校） Cantabria大学交通系西班牙不详

本文介绍一种专门为城市透水性路面而设计的混凝土路面砖的块型。Cantabria大学与当地一家生产企业合作,并得到地方政府的支持,其目的是在西班牙北部应用透水型路面砖作为城市可持续性排水系统。在提出多个不同外形和尺寸的块型设计方案... 详细信息

本文介绍一种专门为城市透水性路面而设计的混凝土路面砖的块型。Cantabria大学与当地一家生产企业合作,并得到地方政府的支持,其目的是在西班牙北部应用透水型路面砖作为城市可持续性排水系统。在提出多个不同外形和尺寸的块型设计方案后,根据路面开孔率、砖连锁咬合接触面和美学角度,先从理论上进行初步对比,选择一种块型——有四种不同形状的透水槽沟;然后在Cantabia大学公路试验室里进行测试,检测不同形状的开孔对路面透水性能的影响。共进行两组测试,第一组假设路面开孔存在不同堵塞状况和坡度,以确定最佳的开孔形状;第二组用选定的混凝土路面砖,分析透水性路面的水径流。根据初步试验结果,生产企业订制模型、生产了第一批新型混凝土路面砖,并已着手推向市场。新型透水性混凝土路面砖的设计研究过程经历了无数次成功和失败,本文仅为丰富技术探讨内容,无意使其成为技术导则。

关键词：透水性路面砖试验开孔面积连锁接触面美学水径流阻力

The in Vitro Antioxidant Capacities of Hydroalcoholic Extracts from Roots and Leaves of Smallanthus sonchifolius (Yacon) Do Not Correlate with Their in Vivo Antioxidant Action in Diabetic Rats

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Journal of Biosciences and Medicines 2016年第2期4卷 15-27页

作者： Ana Carla Broetto Biazon Mariana Marques Nogueira Wendt Júlia Rosa Moreira Cristiane Vizioli castro Ghizoni Andreia Assunção Soares Sandra da Silva Silveira Anacharis Babeto de Sá-Nakanishi Ciomar Aparecida Bersani Amado Rosane Marina Peralta Adelar Bracht Jurandir Fernando Comar Pharmacy Department Faculdades Integrado Campo Mourao Brazil Biochemistry Department University of Maringá Maringá Brazil Pharmacology and Therapeutics Department University of Maringá Maringá Brazil

Leaf and root extracts of Smallanthus sonchifolius (yacon), have antihyper-glycemic activity and antioxidant properties. The present study aims to compare the in vivo hepatic antioxidant activity of hydroalcoholic extracts of yacon leaves and roots in rats with streptozotocin-induced diabetes in terms of their in vitro antioxidant capacity. Rats were treated during 14 days with 1060 mg·Kg-1 root extract or 400 mg·Kg-1 leaf extract. The latter was richer in phenolics and possessed a much higher in vitro antioxidant activity. Both extracts prevented hyperglycemia in diabetic rats. The liver of diabetic rats presented increased levels of protein carbonyls and ROS and decreased activities of antioxidant enzymes. Treatment with both root and leaf extracts restored the protein carbonyl levels to normality. The root extract also restored the ROS levels to normality, but the leaf extract was not effective. The root extract was also more effective in restoring the activity of at least two important antioxidant enzymes (glucose 6-phosphate dehydrogenase and glutathione peroxidase). In terms of the antioxidant load (which was 17 times lower in the root extract treatment), the in vivo action of the root extract was more effective than the leaf extract in reducing the hepatic oxidative stress that accompanies diabetes.

关键词： Smallanthus sonchifolius Yacon Streptozotocin-Induced Diabetes Liver Oxidative Status

Perinatally Imposed Essential Fatty Acid Deficiency Changes Renal Function of the Adult Rat

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Food and Nutrition Sciences 2014年第20期5卷 1991-1999页

作者： Valdilene S.Ribeiro Edjair V.Cabral Alexsandra R.Silva Silvio F.Pereira-Junior Vera L.M.Lima Vera C.O.Carvalho Leucio D.V.Filho Ana D.O.Paixao Carmen castro-Chaves Department of Physiology and Pharmacology Federal University of PernambucoRecifeBrazil Department of Biochemistry Federal University of PernambucoRecifeBrazil

This study was designed to investigate whether essential fatty acid deficiency early during development could change the content of phospholipids and cholesterol in whole membranes of the kidney and renal function at adult life. For this, female Wistar rats were maintained on a standard diet or on an essential fatty acid deficient diet (EFAD) from the age of 30 days, throughout the pregnancy, at age of 90 days and until the weaning, for evaluation of their offspring. Weanling rats were maintained on a standard diet until the age of 13 weeks. Systolic blood pressure (SBP), glomerular filtration rate (GFR), urinary sodium excretion (UNa+V), positive cells for angiotensin II (Ang II) and cholesterol and phospholipids in whole membranes of the kidney were evaluated. Cholesterol, total phospholipids and the relative content of classes of phospholipids were unaltered in the cortex and medullary kidney. SBP, GFR and UNa+V were also unaltered in the EFAD group. However, the number of positive cells for Ang II in the tubulointerstitial area of the renal cortex was higher in the EFAD group. Therefore, these findings indicated that although cholesterol and phospholipids were unaltered and urinary sodium excretion was unchanged, Ang II expression in the kidney was erroneously programmed and later hindering of renal function was not ruled out.

关键词： Angiotensin II Phospholipids Glomerular Filtration Rate

DNA Vaccines Approach:From Concepts to Applications

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World Journal of Vaccines 2014年第2期4卷 50-71页

作者： Vanessa Bastos Pereira Meritxell Zurita-Turk Tessalia Diniz Luerce Saraiva Camila Prosperi De castro Bianca Mendes Souza Pamela Mancha Agresti Fernanda Alvarenga Lima Vanessa Nathalie Pfeiffer Marcela Santiago Pacheco Azevedo Clarissa Santos Rocha Daniela Santos Pontes Vasco Azevedo Anderson Miyoshi Laboratorio de Genetica Celular e Molecular Instituto de Ciencias BiologicasUniversidade Federal de Minas Gerais(UFMG)Belo HorizonteBrazil Universidade Estadual da Paraiba(UEPB) Joao PessoaBrazil

DNA vaccines are the third generation vaccines based on purified plasmid preparations containing transgenes that encode antigenic/therapeutic proteins or peptides capable of triggering an immune response against a wide range of diseases. This vaccine platform presents several attributes that confer distinct advantages over other vaccine technologies in terms of safety, ease of fabrication and stability. Many aspects, such as antigen expression and especially vector design, are under study because of their great influence on immunogenicity and efficacy of DNA vaccines. In this regard, with the attempt of improving the efficiency of DNA vaccines, co-expression of stimulatory sequences and diverse vector delivery systems are being optimized. With this in mind, this review aims to giving a conceptual approach of DNA vaccines, explaining their mechanisms of action and listing the already licensed veterinary DNA vaccines presented in the market.

关键词： DNA Vaccines Vector Design Delivery Systems Approved DNA Vaccines Bacterial Delivery

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臀部增大术：方法、适应证和置入材料的选择

中国美容整形外科杂志 2013年第9期24卷 I0009-I0010页

作者： Jose Horacio Aboudib Fernando Serra Claudio Cardoso de castro 朱利娜(译) 魏峰(译) 高景恒(译) 袁继龙(译) 张晨(译) 里约热内卢大学整形外科系巴西里约热内卢《中国美容整形外科杂志》编辑部辽宁省人民医院整形外科大连大学附属新华医院整形外科

硅胶置人材料用于臀部增大术已有40年的历史。已报道的许多技术都自称可达到美容的目的，但是许多整形医师认为这些手术是不安全的。本文所描述的方法可减少并发症的发生率，改善最终结果，并提出一种理解和掌握这种手术方法的简单方法... 详细信息

硅胶置人材料用于臀部增大术已有40年的历史。已报道的许多技术都自称可达到美容的目的，但是许多整形医师认为这些手术是不安全的。本文所描述的方法可减少并发症的发生率，改善最终结果，并提出一种理解和掌握这种手术方法的简单方法。以10例新鲜尸体的模拟手术为基础，作者坚定了让手术更简单和更安全的目标。103例患者接受手术，手术由整形外科医师或住院医师完成，住院医师在有经验者的监督下完成手术。3．88％发生血清肿，5．80％手术切口裂开，1例（0．97％）发生感染并取出假体，无血肿发生。患者满意度较高。解剖学研究让我们对臀部深层组织获得了解并增加了手术信心，防止损伤坐骨神经，让假体稳固在置入处。手术方法容易理解和掌握。（循证医学证据等级：Ⅳ级，疗法）

关键词：臀部材料置入适应证手术方法患者满意度整形外科医师损伤坐骨神经