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Hydrodynamics and suspended particulate matter retention in macrotidal estuaries located in Amazonia-semiarid interface(Northeastern-Brazil)

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International Journal of Sediment Research 2020年第4期35卷 417-429页

作者： Vinicius Henrique Maciel dos Santos Francisco Jose da Silva Dias Audalio Rebelo Torres Romulo Araujo Soares Lais Costa Terto Antonio carlos Leal de castro Ricardo Luvizotto Santos Marco Valerio Jansen Cutrim Laboratorio de Hidrodinamica Costeira Estuarina e de Aguas Interiores (LHiCEAI) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA)Cidade Universitaria Dom Delgado Laboratorio de Ictiologia (LabIctio) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ecotoxicologia (LabEcotox) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado Laboratorio de Ficologia (LabFic) Do Programa de Pos-Graduacao Em Oceanografia (PPGOceano) da Universidade Federal Do Maranhao (UFMA) Cidade Universitaria Dom Delgado

The aim of the current study was to determine the nature of the seasonal variability of the Suspended Particulate Matter(SPM) fluxes from the drainage basin to the estuary in a macrotidal region(Northeastern Brazil),and the estuarine response to a seawater intrusion regarding sediment deposition,which will support the understanding of the global transport of materials at the continent-ocean *** structure data was acquired using a Conductivity,Temperature,and Depth(CTD) probe with a sampling frequency of 4 *** particulate material was measured by gravimetric measurements applied to exact filtered volume *** outflows were measured through the use of an Acoustic Doppler Current Profiler(ADCP) with frequency of 1.5 *** horizontal thermal and saline gradients varied from warmer and less saline waters(2014) to cooler and saline waters(2015).The gradient behavior when linked to volume transport and SPM flows,suggests a minimization of the fluvial flows in 2015,easing the advance of coastal water(CW) towards the inner estuary,leading to an inversion of the baroclinic pressure *** bottom saline front,generated by the entrance of coastal water masses,caused an increase in SPM concentrations due to increased fluid density,resuspension of previously deposited sediment,and erosion of *** concentrations of SPM indicate higher volume transport suggesting a hydraulic barrier due to the change/inversion of the baroclinic pressure gradient,resulting in water and material *** deposition was observed during neap tide,while during spring tide the material is resuspended,increasing the concentration,generating cycles of deposition and erosion during the neap-spring *** sediment in suspension that reach the estuary,even with low fluvial volume,stay in this environment forming new islands because of *** deposition rates or sediment cycling,if generated by the hydraulic barrier,may indicate that the flo

关键词： Brazilian Amazonia Materials discharges Macrotidal estuaries Freshwater percentage Residence time

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Evidence for maternal transmission of a putative endosymbiont in the digestive gland of Pomacea canaliculata(Architaenioglossa,Ampullariidae)

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BIOCELL 2017年第2期41卷 59-61页

作者： EDUARDO KOCH ISRAEL A.VEGA ALFREDO castro-VAZQUEZ Departamento de Biología Universidad Nacional de CuyoFacultad de Ciencias Exactas y Naturales Instituto Argentino de Investigaciones de las Zonas Aridas(IADIZA-CCT-Mendoza) Instituto de Fisiología Universidad Nacional de CuyoFacultad de Ciencias Médicas IHEM Universidad Nacional de CuyoCONICET

The digestive gland of the apple snail Pomacea canaliculata lodges two types of pigmented corpuscles(identified as C and K corpuscles)which has been proposed as endosymbiont/*** corpuscular types are always present in the digestive gland of adult snails,they are released into the tubuloacinar lumen and are later expelled in the *** their part,hatchlings lack any C or K corpuscles in the digestive gland as well as in their feces,whereas C corpuscles appear in both the gland and feces within one week after ***,it is possible that the detritivorous hatchlings acquire the putative C-endosymbiont from feces in the sediments where they live,***‘lateral’or‘horizontal’*** possibility was put to test in an experiment in which we prevented any lateral transmission,by a 7-days aseptic culture,with no food,of aseptically obtained *** the end of the experiment,we observed that most juveniles had survived the culture period,and hence the digestive glands and feces of survivors were studied by light microscopy of resin embedded,toluidine blue-stained *** studied glands and fecal samples showed C *** is concluded that lateral transmission of the endosymbiont,if any,is not indispensable for the acquisition of the endosymbiont by hatchlings.

关键词： Pigmented corpuscles Ampullariidae Endosymbiosis Coevolution

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Multi-conductor Line Models for Harmonic Load-flow Calculations in LV Networks with High Penetration of PV Generation

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Journal of Modern Power Systems and Clean Energy 2022年第5期10卷 1288-1301页

作者： Fernando M.Camilo M.E.Almeida Rui castro V.Fernao Pires Institutode Engenhariade Sistemas e Computadores Investigacao eDesenvolvimento(INESC-ID)and TecnicoUniversity of LisbonLisbonPortugal ESTSetubal Polytechnics of SetubalSetuibalPortugal

Low-voltage(LV) distribution networks are unbalanced and present loads with nonlinear behavior, which introduce harmonics in the networks. The predictable increase in photovoltaic microgeneration(PV μG) accentuates this unbalanced characteristic, as well as poses new technical problems,namely voltage rise and reverse power flow. To accurately account for the distributed PV and loads in the LV network, unbalanced three-phase power flow algorithms should be utilized,where different approaches may be used to represent lines with various degrees of accuracy. The more accurate algorithm considers the electromagnetic coupling between the line conductors,whereas the simpler algorithm represents each conductor of the line as a single-phase line with pure resistive behavior. This paper aims to analyze the influence of the line model on the load flow in a highly unbalanced LV network with a high penetration of PV production, and considers the impact of the harmonics produced by nonlinear loads. Based on the results obtained,it is possible to identify the most suitable model to be used, depending on the study to be performed. Different scenarios of PV generation and loads are addressed in this paper.

关键词： Line model nonlinear loads photovoltaic microgeneration unbalanced distribution network

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Methodological Guidelines for the Use of Geoprocessing Tools:Spatial Analysis Operations—Kernel,Buffer and the Remote Sensing Image Classification

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Agricultural Sciences 2015年第7期6卷 707-716页

作者： Christian Nunes da Silva Joao Marcio Palheta da Silva carlos Jorge Nogueira castro Federal University of Pará (UFPA)BelémBrazil

The so-called geotechnology has been used in recent years in the planning, supervision and monitoring of various human activities, both locally and regionally, nationally and internationally, either it in the rural environment, as in urban áreas. This study, based on references and activities in the Geographic Information Analysis Laboratory of the Federal University of Pará (LAIG/UFPA), selects and presents the application of three tools for spatial analysis available in the Terraview [1] and Arcgis softwares, with the main objective being to demonstrate how they can be applied in geographical studies, starting with the spatial information gathered by remote or Field sensors, assisting the activities of researchers who stick to working with the planning and management of natural and human resources. Geotechnologies are important tools in the analysis of geographic space and its use tends to be increased with the advancement of new software and hardware collection, manipulation and generation of new specialized information. So we will do in this manuscript a brief discussion of three spatial analysis tools that can be used in rural areas with agricultural potential.

关键词： Geotechnology Geoprocessing Spatial Analysis

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Hepatobiliar manifestations in inflammatory bowel disease: A practical approach

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World Journal of Hepatology 2022年第2期14卷 319-337页

作者： Paulina Núñez F Fabiola castro Gabriel Mezzano Rodrigo Quera Diego Diaz Lorena castro Universidad de los Andes Inflammatory Bowel Disease ProgramDigestive Disease CenterSantiago 7600976RMChile Inflammatory Bowel Disease Program Hospital San Juan de DiosUniversidad de ChileSantiago 7701230RMChile Universidad de los Andes Hepatology ProgramDigestive Disease CenterSantiago 7600976RMChile Department of Gastroenterology Hospital del Salvador/Universidad de ChileSantiago 7600976RMChile Universidad de los Andes Santiago 770976RMChile

Inflammatory bowel diseases(IBD)are associated with various hepatobiliary *** can occur at any moment in the course of the disease or associated with the *** prevalence of liver dysfunction can reach up to 50%in different *** fatty liver disease is considered the most common hepatobiliary complication in IBD,while primary sclerosing cholangitis is the most *** of hepatic manifestations in IBD involves a multidisciplinary approach that includes a high index of suspicion and joint management with *** medical confrontation with abnormal liver tests must include an exhaustive study to determine if these patterns can be related to IBD,associated diseases or to the therapies used.

关键词： Extraintestinal manifestations Inflammatory bowel disease Sclerosing primary cholangitis Hepatic steatosis Liver toxicity

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Predictive factors associated with hepatitis C antiviral therapy response

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World Journal of Hepatology 2015年第12期7卷 1617-1631页

作者： Lourianne Nascimento Cavalcante André castro Lyra Hospital Sao Rafael-Gastro-Hepatology Service Department of Medicine Federal University of Bahia

Hepatitis C virus(HCV) infection may lead to significant liver injury, and viral, environmental, host, immunologic and genetic factors may contribute to the differences in the disease expression and treatment response. In the early 2000 s, dual therapy using a combinationof pegylated interferon plus ribavirin(PR) became the standard of care for HCV treatment. In this PR era, predictive factors of therapy response related to virus and host have been identified. In 2010/2011, therapeutic regimens for HCV genotype 1 patients were modified, and the addition of NS3/4a protease inhibitors(boceprevir or telaprevir) to dual therapy increased the effectiveness and chances of sustained virologic response(SVR). Nevertheless, the first-generation triple therapy is associated with many adverse events, some of which are serious and associated with death, particularly in cirrhotic patients. This led to the need to identify viral and host predictive factors that might influence the SVR rate to triple therapy and avoid unnecessary exposure to these drugs. Over the past four years, hepatitis C treatment has been rapidly changing with the development of new therapies and other developments. Currently, with the more recent generations of pangenotipic antiviral therapies, there have been higher sustained virologic rates, and prognostic factors may not have the same importance and strength as before. Nonetheless, some variables may still be consistent with the low rates of non-response with regimens that include sofosbuvir, daclatasvir and ledipasvir. In this manuscript, we review the predictive factors of therapy response across the different treatment regimens over the last decade including the new antiviral drugs.

关键词： Hepatitis C Direct acting antivirals Antiviraltherapy Interferon Sustained virologic response

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HPV18 Utilizes Two Alternative Branch Sites for E6*I Splicing to Produce E7 Protein

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Virologica Sinica 2019年第2期34卷 211-221页

作者： Ayslan castro Brant Vladimir Majerciak Miguel Angelo Martins Moreira Zhi-Ming Zheng Tumor Virus RNA Biology Section RNA Biology Laboratory Center for Cancer Research National Cancer Institute NIH Frederick MD 21702 USA Genetics Post-Graduation Program Rio de Janeiro Federal University Rio de Janeiro Brazil Genetics Program Nacional Cancer Institute INCA Rio de Janeiro 20231-050 Brazil

Human papillomavirus 18(HPV18) E6 and E7 oncogenes are transcribed as a single bicistronic E6 E7 pre-mRNA. The E6 ORF region in the bicistronic E6 E7 pre-mRNA contains an intron. Splicing of this intron disrupts the E6 ORF integrity and produces a spliced E6*I RNA for efficient E7 translation. Here we report that the E6 intron has two overlapped branch point sequences(BPS) upstream of its 30 splice site, with an identical heptamer AACUAAC, for E6*I splicing. One heptamer has a branch site adenosine(underlined) at nt 384 and the other at nt 388. E6*I splicing efficiency correlates to the expression level of E6 and E7 proteins and depends on the selection of which branch site. In general, E6*I splicing prefers the 30 ss-proximal branch site at nt 388 over the distal branch site at nt 384. Inactivation of the nt 388 branch site was found to activate a cryptic acceptor site at nt 636 for aberrant RNA splicing. Together, these data suggest that HPV18 modulates its production ratio of E6 and E7 proteins by alternative selection of the two mapped branch sites for the E6*I splicing, which could be beneficial in its productive or oncogenic infection according to the host cell environment.

关键词： Human papillomavirus 18 (HPV18) HPV splicing Branch point E6,E7,E6 intron HPV oncogenes

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Solutions for submucosal injection: What to choose and how to do it

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World Journal of Gastroenterology 2019年第7期25卷 777-788页

作者： Rui castro Diogo Libanio Inês Pita Mário Dinis-Ribeiro Department of Gastroenterology Portuguese Oncology Institute of Porto CIDES/CINTESIS Faculty of Medicine University of Porto

During the past decades, endoscopic resection techniques have gradually improved and gained more importance for the management of premalignant lesions and early cancers. These endoscopic resection techniques can be divided in 3 major groups: snare polipectomy, endoscopic mucosal resection(EMR) and endoscopic submucosal dissection(ESD). The use of submucosal injection is essential for the majority of EMR techniques and is an integral part of ESD,whereas during polipectomy it is not crucial in most cases except to prevent bleeding in large polyps and/or those with large stalks as an alternative to mechanical methods. Injection provides a lifting up effect of the lesion separating it from the muscular layer, thereby reducing thermal injury and the risk of perforation and bleeding while also facilitating en-bloc resection by improving technical feasibility. With this work, we aim to review the most common endoscopic resection techniques and the importance of submucosal injection in each one of them. For that, we present some of the most commonly used submucosal injection solutions, taking into account their advantages and disadvantages. We also discuss, based on current recommendations and our own experience, how and when to preform submucosal injection, depending on lesions features and endoscopic resection technique that′s being used, to assure complete resection and to prevent associated adverse events. Finally, we also present and discuss some new proposed submucosal injection solutions,endoscopic resection techniques and devices that may have a major impact on the future of therapeutic endoscopy.

关键词： Snare polipectomy Endoscopic mucosal resection Endoscopic submucosal dissection Submucosal injection Submucosal injection solution

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Total segmental aplasia of uterus body in bitch

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Asian pacific Journal of Reproduction 2019年第1期8卷 43-44页

作者： Leonardo Martins Leal Fábio Rodrigo castro Bastos Tais Harumi de castro Sasahara Paola castro Moraes Márcia Rita Fernandes Machado Centro Universitário Ingá-UNINGá MaringáPRBrazil Universidade de Sao Paulo-USP Sao PauloSPBrazil Universidade Estadual Paulista-Unesp JaboticabalSPBrazil

Congenital anomalies of the female tubular genital tract are rare and known as segmental aplasia, resulting from incomplete development of paramesonephric ducts during embryonic stage, which originate from the cranial portion of vagina, cervix, uterus and oviducts. In the literature consulted, several reports of uterine horn aplasia are described in bitches, however there is only one report described in the world literature on total aplasia of the body of the uterus. This study aimed to describe the case of a two years old bitch, no breed defined, with 12 kg of weight, with segmental aplasia of uterus body diagnosed during ovariohysterectomy. During surgery it was noted that the bitch had the ovaries and uterine horns in their anatomical position, however an aplasia was noted in the body of the uterus. It was also found that the uterine horns had liquid inside, suggesting hydrometra. It was concluded in this case that although segmental aplasia of the body of the uterus is rare and difficult to diagnose by maintaining normal cyclicity of the female, it should be considered in dogs that do not have bloody vaginal discharge in proestrus and can not become pregnant after natural coverage or artificial insemination.

关键词： Segmental aplasia Surgery Ovariohysterectomy Reproduction

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Methods used in microbial forensics and epidemiological investigations for stronger health systems

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Forensic Sciences Research 2022年第4期7卷 650-661页

作者： Arizaldo E.castro Maria Corazon A.De Ungria Microbial Ecology of Terrestrial and Aquatic Systems Laboratory Institute of BiologyUniversity of the Philippines DilimanQuezon CityPhilippines DNAAnalysis Laboratory Natural Sciences Research InstituteUniversity of the Philippines DilimanQuezon CityPhilippines Program on Biodiversity Ethnicityand ForensicsPhilippine Genome CenterUniversity of the Philippines DilimanQuezon CityPhilippines

This review discusses microbial forensics as an emerging science that finds application in protecting human *** is important to distinguish naturally acquired infections from those caused by the intentional release of microorganisms to the *** information is crucial in formulating procedures against the spread of infectious diseases and prosecuting persons who may be involved in acts of biocrime,bioterrorism,or biowarfare.A comparison between epidemiological investigations and microbial forensic investigations is *** addition,a discussion on how microbial forensics strengthens health systems is included in this *** forensic investigations and epidemiologic examinations employ similar concepts and involve identifying and characterising the microbe of *** fields require formulating an appropriate case definition,determining a pathogen’s mode of transmission,and identifying the source(s)of ***,the two subdisciplines differ in their *** epidemiological investigation aims to identify the pathogen’s source to prevent the spread of the *** forensics focuses on source-tracking to facilitate the prosecution of persons responsible for the spread of a *** fields use molecular techniques in analysing and comparing DNA,gene products,and biomolecules to identify and characterise the microorganisms of *** included case studies to show methods used in microbial forensic investigations,a brief discussion of the public significance of microbial forensic systems,and a roadmap for establishing a system at a national *** system is expected to strengthen a country’s capacity to respond to public health *** factors must be considered in establishing national microbial forensic *** is the inherent ubiquity,diversity,and adaptability of microorganisms that warrants the use of robust and accurate molecular typing ***,the availability of facilit

关键词： Forensic sciences microbial forensics epidemiology infectious diseases molecular methods health system resilience microbial forensic systems

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