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Polymorphism in the Regulatory Region of the Aromatase CYP19a Gene in Nile Tilapia

Journal of Life Sciences 2014年第2期8卷 101-105页

作者： Marilia Danyelle Nunes Rodrigues Rafael Aldrighi Tavares Harold Julian Perez Gutierrez Diones Bender Almeida carla giovane avila moreira Cecilia Calabuig Josiane Bonel Raposo Heden Luiz Marquesmoreira Department of Animal Science Federal University of Pelotas Pelotas 96160-000 Rio Grande do Sul Brazil Institute of Biology Department of Zoology and Genetics Federal University of Pelotas Pelotas 96160-000 Brazil Institute of Biology Federal University of Rio Grande do Sul Porto Alegre 91501-970 Brazil Department of Animal Sciences Federal Rural University of the Semi-Arid Mossoro 59625-900 Brazil Faculty of Veterinary Department of Pathology Federal University of Pelotas Pelotas 96160-000 Brazil

The aim of this study was to evaluate the polymorphism in a portion of the gene regulatory region for ovarian aromatase （CYP19a） in three strains of Tilapia, Oreochomis niloticus （Linnaeus）（GIFT--Genetically Improved Farmed Tilapia, Chitralada and Supreme）. A total of 90 animals per strain of Tilapia, Oreochromis niloticus （Linnaeus） were analysed. After DNA extraction, samples were subjected to PCR using primers designed to flank the region of interest encompassing the sites of transcription （WT1-KTS and SRY）. Samples were analyzed by PCR-SSCP and subsequently sequenced. Three polymorphisms were identified in this region, resulting in two different sequences, in the GIFT strain while no polymorphism was found in both Supreme and Chitralada strains. At the position - 1178 the substitution of a guanine for a cytosine, at the - 1081 the exchange of guanine for adenine and at the position -1 138 we found a SNP, possible site of heterozygosity. Even with polymorphisms in the target study area, when taking the three strains into account, one can assume that the portion of the regulatory region of the ovarian aromatase gene in the Supreme strain and Chitralada does not show polymorphism.

关键词： Ovarian aromatase Oreochromis niloticus polymorphism regulatory region.

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Red Rice Control and Soybean Tolerance to <i>S</i>-Metolachlor in Association with Glyphosate

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American Journal of Plant Sciences 2014年第13期5卷 2040-2047页

作者： carla Rejane Zemolin Luis Antonio de avila Dirceu Agostinetto Guilherme Vestena Cassol Marlon Bastiani Rodrigo Pestana Crop Protection Department Federal University of Pelotas Pelotas Brazil

Red rice is one of the major troublesome and difficult weeds to control in rice production regions. The introduction of the Clearfield® technology allowed producers to control red rice using rice genotypes tolerant to the imidazolinone herbicides. However, because the consecutive use of this technology red rice biotypes have evolved resistance to imidazolinone herbicides, the rice-soybean rotation has been an alternative used by producers to control this weed. This system allows the use of herbicides with different modes of action to control red rice, such as S-metolachlor. Thus, greenhouse and field experiments were carried out during the 2011 to 2012 and 2012 to 2013 growing seasons to evaluate: 1) sensitivity of imidazolinone-resistant red rice to S-metolachlor;2) red rice control and soybean tolerance in response to associations of S-metolachlor and glyphosate. In greenhouse, S-metolachlor effectively controlled both susceptible and imidazolinone-resistant red rice in preemergence. In field, preemergence applications of S-metolachlor provided greater red rice control in comparison to S-metolachlor alone in early postemergence. The association of S-metolachlor with glyphosate did not improve red rice control in preemergence application. However, association of S-metolachlor with glyphosate significantly improved red rice control in early postemergence applications. S-metolachlor injury to soybean increased with early postemergence applications. These results indicate that S-metolachlor effectively control imidazolinone-resistant red rice in rice-soybean rotation.

关键词： Crop Rotation Weed Control Crop Injury Application Timing

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Single Step Microwave Assisted Synthesis and Antimicrobial Activity of Silver, Copper and Silver-Copper Nanoparticles

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Journal of Materials Science and Chemical Engineering 2020年第8期8卷 13-29页

作者： Myrna Reyes-Blas Nadja M. Maldonado-Luna carla M. Rivera-Quiñones Ana L. Vega-avila Felix R. Roman-Velázquez Oscar J. Perales-Perez Department of Chemistry University of Puerto Rico at Mayagüez Mayaguez USA Department of Mechanical Engineering University of Puerto Rico at Mayagüez Mayaguez USA Department of Chemical Engineering University of Puerto Rico at Mayagüez Mayaguez USA Department of Engineering Science & Materials University of Puerto Rico at Mayagüez Mayaguez USA

Copper and silver nanoparticles were synthesized and characterized in two minutes at 175°C in a one-step synthesis using a modified polyol (ethylene glycol) method and a microwave heating process. We successfully synthesized spherical Silver (Ag) and Copper nanoparticles (CuNP) with a crystallite size of less than 10 nm, as well as irregular silver-copper nanoparticles (AgCuNP) with a crystallite size of less than 15 nm, as confirmed by X-Ray Diffraction (XRD) and High-Resolution Transmission Electron Microscopy (HRTEM). The successful synthesis of AgCuNP with 1:1 molar ratio and constituted by 51.74% of copper and 48.26% of silver was corroborated using the Energy Dispersive X-ray (EDX) mapping technique. The AgNP and AgCuNP exhibited more stability in suspension, in comparison to CuNP, as observed by continuously monitoring the absorbance with UV-Vis spectroscopy for 12 days. Furthermore, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of AgNP, CuNP, and AgCuNP were determined, against Gram-negative and Gram-positive bacteria, and yeast. The obtained MIC and MBC values indicate that AgCu nanoparticles exhibited bactericidal properties greater than its constituents. On the contrary, antifungal activity of AgCuNP against yeast was not observed.

关键词： Ag Nanoparticles Cu Nanoparticles Microwave Synthesis Modified Polyol Method Antimicrobial Properties AgCu Nanoparticles

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