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Chronic hepatitis B liver disease in patients living in the Amazon region: S gene mutations and genotypes characterization

引用

World Journal of Cardiovascular Diseases 2013年第8期3卷 506-511页

作者： Deusilene Vieira Marie Gauthier Larissa Deadame de Figueiredo Nicolete Alcione Oliveira dos Santos carina picelli Eduardo Honda Glaucia Paranhos-Baccala Guy Vernet Juan Miguel Villalobos Salcedo Research Center for Tropical Medicine CEPEM/Tropical Pathology Research InstituteFiocruz RondoniaRio de JaneiroBrazil Emerging Pathogens Laboratory Fondation MerieuxIFR 128 BioSciences GerlandLyonFrance

The Amazon region is considered to be a high endemic area for Hepatitis B Virus (HBV) infections, Rondônia state having the highest prevalence. The aim of this study was to identify molecular genotypes and mutations in the S gene region of HBV viral genomes from 20 patients using a DNA microarray. Results: Serological tests showed that 88% of patients were HBeAg negative, 82% had anti-HBe antibodies and 33% were co-infected with Hepatitis Delta Virus. Sixteen percent of the patients were considered cirrhotic, and 11% have been transfused. The microarray technique identified the genotypes A (4 patients), D (7 patients) and F (7 patients) in 18 samples. Mutations were detected in all 3 genotypes and, overall, A159G, which has been associated with a reduced antigenicity of the virus, was detected most frequently. In genotype A, G119E was the most frequently detected mutation followed by mutations A159G, F134Y, W172C, Y161F and T143S. A159G was detected in all genotype D and F samples followed by mutations T143S, Y161F, N131T, T114S and G119E in genotype D and mutations T143S, Y161F, N131T, T114S and G119E in genotype F. Conclusion: The analysis of mutations repartition among genotypes suggests that some of them are preferentially or exclusively associated with genotype A, D or F. This type of tool is adapted for clinical and therapy monitoring of patient as well as for molecular epidemiology research on HBV.

关键词： Hepatitis B Virus Mutant Detection DNA Microarray Amazon Region

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