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Toluene Biodegradation by Novel Bacteria Isolated from Polluted soil surrounding car Body Repair and spray Painting Workshops

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Journal of Environmental Protection 2015年第12期6卷 1417-1429页

作者： jacob H. jacob Fawzi I. Irshaid Department of Biological sciences Faculty of Science Al al-Bayt University Al-Mafraq Jordan

Toluene can enter the environment due to various industrial activities. Toluene exposure may cause serious health risks to human and other living organisms. Thus, removal of toluene from the environment is exceptionally important in toluene-contaminated habitats. The aim of present study was to isolate and characterize toluene-utilizing bacteria from contaminated soil surrounding car body repair and painting workshops in Irbid industrial city, Jordan. Therefore, polluted soil samples (10 g) were transferred to flasks containing 99 ml of stanier’s medium supplemented with 1% toluene and aerobically incubated at 30°C for 72 h. subsequently, four morphologically different toluene-utilizing bacteria, designated as T1, T2, T3, and T4, were isolated. The cells of T1, T2 and T3 were Gram-positive, rod-shaped, aerobic, and positive for oxidase and catalase. However, the cells of T4 were Gram-negative, round-shaped, aerobic, negative for oxidase, and positive for catalase. Based on 16s rDNA sequencing data, isolates T1, T2 and T3 had high homology (97% - 98%) with Lysinibacillus boronitolerans, Bacillus subtilis and Rhodococcus pyridinivorans, respectively, whereas the isolate T4 had a homology of 89% with Acinetobacter schindleri, and could represent a distinct lineage within the genus Acinetobacter. The generated 16s rDNA sequences were deposited in GenBank database. After testing different physicochemical conditions, the isolates appeared to grow best at 1% toluene, 30°C and pH 6.8, with the generation times ranged between 8 - 11 h. In conclusion, we reported for the first time the identification of four novel soil bacterial species with the capacity to utilize toluene as sole source of carbon and energy from soil sites surrounding car painting workshops. The beneficial effect of the four isolates in the bioremediation of toluene from toluene polluted soil areas must be examined under in situ and ex situ conditions.

关键词： Toluene Biodegradation soil Pollution Lysinibacillus Rhodococcus Acinetobacter

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Penetrating and Basilic Trauma of the Thorax in a Context of Difficult Diagnosis at the Pediatric Hospital of Bangui (Central African Republic)

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Open Journal of Pediatrics 2023年第5期13卷 657-662页

作者： Daniel sylvère Ouaïmon jacob Israël Junior sopio Nathan Gbanade Packo Oumarou Youssof Center Hospitalier Universitaire Pediatrique de Bangui (CHUPB) Bangui Central African Republic Faculty of Health sciences Bangui University Bangui Central African Republic

Appropriate management of penetrating trauma to the thorax requires knowledge of vulnating agents, as well as the principles of ballistic injury. The importance of the approach’s choice for surgical exploration of these injuries, and parietal damage, is an essential factor in decision making in the management and definition of the therapeutic strategy for these injuries. The authors report a clinical case of a penetrating traumatic ballistic wound of the thorax managed in a context of difficult diagnosis in the surgical Unit of the CHUPB.

关键词： Firearm Wound Thorax Trauma Child car

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滨海地区地下水泄流研究进展

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化工矿产地质 2011年第1期33卷 50-53页

作者：李丹王刚丁丽娜石磊辽宁省化工地质勘查院辽宁锦州121000 辽宁工程技术大学辽宁阜新123000

20世纪90年代以来出现的对滨海地下水的研究方式取得了有意义的结果。其中***通过对226Ra富集揭示了地下水对海岸水体大量补给的研究以及jacob、sun等关于潮夕动力地下水流法的研究对解决环境地质问题具有一定的意义。

关键词：地下水滨海解析法同位素

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