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Does the European Union Need More Democracy： a Reconsideration of ＂Democratic Deficit＂ and a New Analysis Framework

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学术界 2017年第10期 257-267页

作者： cao meng School of Government Peking University Beijing 100871

There has been a long-standing debate on the European Union's democratic deficit. The issue was even more complicated today during the hard time of the European crises. However,a no-solution predicament inevitably emerged under the traditional democratic deficit paradigm. This article proposed a new perspective regarding democracy and a new analysis framework to check the EU's institutional arrangement,under which the EU's democratic issue could be acceptably addressed.

关键词：民主欧盟框架欧洲联盟字问题

Feature Relationships Learning Incorporated Age Estimation Assisted by Cumulative Attribute Encoding

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Computers, Materials & Continua 2018年第9期56卷 467-482页

作者： Qing Tian meng cao Tinghuai Ma School of Computer and Software Nanjing University of Information Science and TechnologyNanjing210044China Jiangsu Engineering Center of Network Monitoring Nanjing University of Information Science and TechnologyNanjing210044China School of Electrical and Electronic Engineering University of ManchesterManchesterM139PLUK

The research of human facial age estimation(AE)has attracted increasing attention for its wide *** to date,a number of models have been constructed or employed to perform *** the goal of AE can be achieved by either classification or regression,the latter based methods generally yield more promising results because the continuity and gradualness of human aging can naturally be preserved in age ***,the neighbor-similarity and ordinality of age labels are not taken into account *** overcome this issue,the cumulative attribute(CA)coding was *** such age label relationships can be parameterized via CA coding,the potential relationships behind age features are not incorporated to estimate *** this end,in this paper we propose to perform AE by encoding the potential age feature relationships with CA coding via an implicit modeling *** that,we further extend our model to gender-aware AE by taking into account gender variance in aging ***,we experimentally validate the superiority of the proposed methodology.

关键词： Age estimation cumulative attribute gender-aware age estimation correlation relationship learning

Coverage analysis for sensor networks based on Clifford algebra

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Science in China(Series F) 2008年第5期51卷 460-475页

作者： XIE WeiXin cao WenMing meng Shan ATR Key Laboratory of Defense Technology Shenzhen University Shenzhen 518060 China

The coverage performance is the foundation of information acquisition in distributed sensor networks. The previously proposed coverage work was mostly based on unit disk coverage model or ball coverage model in 2D or 3D space, respectively. However, most methods cannot give a homogeneous coverage model for targets with hybrid types. This paper presents a coverage analysis approach for sensor networks based on Clifford algebra and establishes a homogeneous coverage model for sensor networks with hybrid types of targets. The effectiveness of the approach is demonstrated with examples.

关键词： sensor network Clifford algebra coverage analysis rotation operator distance measure

Improvement in Mechanical Properties and Biocompatibility ofBiosynthetic Bacterial Cellulose/Lotus Root Starch Composites

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Chinese Journal of Polymer Science 2017年第3期35卷 354-364页

作者： Na Yin 陈仕艳 Yi-meng cao Hua-ping Wang 吴氢凯 State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and Engineering Donghua University Shanghai 201620 China Department of Obstetrics and Gynecology Affiliated Sifcth People's Hospital Shanghai Jiaotong University Shanghai 200233 China

Bacterial cellulose/lotus root starch(BC/LRS) composites were prepared by cultivating Acetobacter xylinum in nutrient media containing gelatinized lotus root starch. Low concentrations of gelatinized LRS had increased BC production with the maximum value at 6.67 g/L when 5 g/L of LRS was added in the culture media and the composites had thicker and denser fibrils compared with those of BC with low concentrations of LRS(2.5 and 5 g/L). When the concentration of LRS was increased above 7.5 g/L, the morphology of the BC/LRS composites contained more fibril layers that were linked with LRS. The results from X-ray diffraction(XRD) demonstrated that there was no significant difference in structure between BC and BC/LRS composites except a slight increase in crystallinity for BC/LRS composites as the concentration of LRS was lifted up. The tensile tests were performed to display BC/LRS composites prepared with LRS concentration at 2.5 and 5 g/L in media had the tensile strength of 54 and 60 MPa, respectively, which indicated an improvement in mechanical property compared to the unmodified BC(45 MPa). Live/dead assay with chondrocytes seeded on BC/LRS composite revealed higher cell viability ranging from 85% to 90% than BC. Furthermore, cell morphology with typical spindle shape was observed on the surfaces of BC/LRS composite by confocal microscope. Through the overall results, it shows that this study has provided a guidance to prepare BC/LRS composites with better cell biocompatibility and higher mechanical strength than those of BC for the potential use in cartilage tissue engineering.

关键词： Bacterial cellulose Lotus root starch Composites Biosynthesis Cell biocompatibility

Flow Behaviour Analysis and Experimental Investigation for Emitter Micro-channels

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Chinese Journal of Mechanical Engineering 2012年第4期25卷 729-737页

作者： WEI Zhengying cao meng LIU Xia TANG Yiping LU Bingheng State Key Lab for Manufacturing System Engineering Xi 'an Jiaotong University Xi 'an 710049 China

The existing research of the flow behavior in emitter micro-channels mainly focuses on the single-phase flow *** the recent micro-particle image velocimetry（PIV） experimental research on the flow characteristics in various micro-channels mainly focuses on the single-phase fluid ***,using an original-size emitter prototype to perform the experiments on the two-phase flow characteristics of the labyrinth channels is seldom *** this paper,the practical flow of water,mixed with sand escaped from filtering,in the labyrinth channel,is *** some research work on the clogging mechanism of the labyrinth channel＇s structure is *** fluid dynamics（CFD） analysis has been performed on liquid-solid two-phase flow in labyrinth-channel *** on flow visualization technology-micro-PIV,the flow in labyrinth channel has been photographed and *** path line graph and velocity vector graph are obtained through the post-treatment of experimental *** graphs agree well with CFD analysis results,so CFD analysis can be used in optimal design of labyrinth-channel *** the optimized anti-clogging structures of the rectangular channel and zigzag channel have been designed *** CFD numerical simulation and the micro-PIV experiments analysis on labyrinth-channel emitter,make the ＂black box＂ of the flow behavior in the emitter channel ***,the proposed research promotes an advanced method to evaluate the emitter＇s performance and can be used to conducting the optimal design of the labyrinth-channel emitters.

关键词： drip irrigation emitter labyrinth channel two-phase flow analysis anti-clogging micro-PIV

A CRISPR/RfxCas13d-mediated strategy for efficient RNA knockdown in mouse embryonic development

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Science China(Life Sciences) 2024年第11期67卷 2297-2306页

作者： Lin Zhang Shi-meng cao Hao Wu meng Yan Jinsong Li Ling-Ling Chen Key Laboratory of RNA Innovation Science and EngineeringCAS Center for Excellence in Molecular Cell ScienceShanghai Institute of Biochemistry and Cell BiologyUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCenter for Excellence in Molecular Cell ScienceChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghai 200031China Key Laboratory of Systems Health Science of Zhejiang Province School of Life ScienceHangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China New Cornerstone Science Laboratory Shenzhen 518054China

The growing variety of RNA classes,such as mRNAs,lncRNAs,and circRNAs,plays pivotal roles in both developmental processes and various pathophysiological ***,our comprehension of RNA functions in live organisms remains limited due to the absence of durable and effective strategies for directly influencing RNA *** this study,we combined the CRISPR-RfxCas13d system with spermlike stem cell-mediated semi-cloning techniques,which enabled the suppressed expression of different RNA *** approach was employed to interfere with the expression of three types of RNA molecules:Sfmbt2 mRNA,Fendrr lncRNA,and circMan1a2(2,3,4,5,6).The results confirmed the critical roles of these RNAs in embryonic development,as their loss led to observable phenotypes,including embryonic lethality,delayed embryonic development,and embryo *** summary,our methodology offers a potent toolkit for silencing specific RNA targets in living organisms without introducing genetic alterations.

关键词： sperm-like stem cell semi-cloned technology CRISPR-RfxCas13d RNA knockdown embryonic development

Leakage Current Simulation of Insulating Thin Film Irradiated by a Nonpenetrating Electron Beam

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Chinese Physics Letters 2012年第4期29卷 221-224页

作者： ZHANG Hai-Bo LI Wei-Qin cao meng Key Laboratory for Physical Electronics and Devices of MOE Department of Electronic Science and TechnologyXi’an Jiaotong UniversityXi’an 710049 School of Automation and Information Engineering Xi’an University of TechnologyXi’an 710048

We perform numerical simulations of the leakage current characteristics of an insulating thin film of SiO2 negatively charged by a low-energy nonpenetrating focused electron *** the formation of leakage current,electrons are demonstrated to turn from diffusion to drift after clearing the minimum potential barrier due to electron-hole *** the equilibrium state,the leakage current increases approximately linearly with the increasing primary beam current and *** also increases with the increasing film thickness and trap density,and with the decreasing electron mobility,in which the film thickness has a greater *** by some existing experiments,the simulation results provide a new perspective for the negative charging effects of insulating samples due to the low-energy focused electron beam.

关键词： film Electron linearly

LISR28 Is Involved in Pollen Germination by Affecting Filamentous Actin Dynamics

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Molecular Plant 2013年第4期6卷 1163-1175页

作者： Li-Juan cao meng-meng Zhao Chang Liu Huai-Jian Dong Wang-Cheng Li Hai-Yun Ren Key Laboratory of Cell Proliferation and Regulation Biology of Ministry of Education College of Life Science Beijing Normal University Beijing 100875China

Alternative splicing plays important roles in gene regulation and contributes to protein complexity. Previous studies suggest that alternative splicing exists in members of the villin/gelsolin/fragmin superfamily. In this study, a ser- ine/argine-rich （SR） protein cDNA with 28kDa protein （LISR28） was isolated from a lily （Lilium Iongiflorum） expression library. Protein domain analysis showed that LISR28 had similar structures to Arabidopsis SR45 （AtSR45）, and LISR28 could complement the phenotype of loss of AtSR45 function. Therefore, overexpression of LISR28 and AtSR45 mutant （atsr45-1） were used in the following experiments. Overexpression of LISR28 in Arabidopsis completely inhibited pollen germina- tion. In contrast, the pollen germination of atsr45-1 was earlier than that of wild-type. In addition, pollen of atsr45-1 contained less F-actin at the corresponding hydration stage during pollen germination compared to that of wild-type. Alternative splicing analysis showed that Arabidopsis villinl （AtVLN1） transcript encoding the full-length protein was increased, and that encoding the truncated protein was decreased in atst45-1. Moreover, the mRNA expression level of other actin-binding proteins （ABPs） abundant in Arabidopsis pollen was also changed in atsr45-1. In conclusion, we hypothesize that LISR28 alters F-actin dynamics probably through its alternative splicing activities to affect directly or indirectly the alternative splicing of AtVLN1 and the expression of different ABPs, which then affects the pollen germination.

关键词： SR protein alternative splicing F-actin.

Measurement of Secondary Electron Energy Spectra of Polymethyl Methacrylate

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Chinese Physics Letters 2018年第4期35卷 108-112页

作者： Ming Weng Wan Liu Ming Yin Fang Wang meng cao Key Laboratory for Physical Electronics and Devices (Ministry of Education) Department of Electronic Science and Technology Xi'an Jiaotong University

We report on a novel and convenient method of measuring secondary electron spectra for insulators in a secondary electron yield measurement system with a planar grid analyzer configuration and a metal mesh probe. In this measurement, the planar grid is negatively biased to force some emitted secondary electrons to return to the sample surface and to neutralize charges accumulated on the sample during the previous beam irradiation. The surface potential of the sample is then measured by use of a metal mesh probe. The grid bias for neutralization corresponding to the zero surface potential is determined based on the linear relationship between the surface potential and the grid bias. Once the surface potential equals zero, the secondary electron spectra of polymethyl methacrylate（PMMA） are studied experimentally by measuring the -curve and then fitting it to Everhart＇s formula. The measurement results show that the peak energy and the full width at half maximum of the spectra are 4.26 eV and 14.06 eV, respectively.

关键词： SE In Measurement of Secondary Electron Energy Spectra of Polymethyl Methacrylate