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青海湖裸鲤TOB1和TOB2基因的克隆与表达分析

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西北农林科技大学学报（自然科学版） 2020年第5期48卷 31-41页

作者：梁健陈雪妍卫唯马清花才项卓玛青海大学省部共建三江源生态与高原农牧业国家重点实验室青海西宁810016 青海大学生态环境工程学院青海西宁810016

【目的】对青海湖裸鲤TOB1和TOB2基因进行克隆及表达分析,为揭示其在青海湖裸鲤生长和生理过程中的作用奠定基础。【方法】采用PCR和RACE技术克隆青海湖裸鲤中TOB1和TOB2基因的cDNA全长序列,对其特性进行了生物信息学分析。以β-actin... 详细信息

【目的】对青海湖裸鲤TOB1和TOB2基因进行克隆及表达分析,为揭示其在青海湖裸鲤生长和生理过程中的作用奠定基础。【方法】采用PCR和RACE技术克隆青海湖裸鲤中TOB1和TOB2基因的cDNA全长序列,对其特性进行了生物信息学分析。以β-actin为内参基因,采用实时荧光定量PCR法(qRT-PCR)检测TOB1和TOB2基因在3龄成鱼肾脏、肠、鳃、脑、心脏、肌肉等组织及发育12,72,120,168,216 h胚胎中的表达水平。【结果】青海湖裸鲤TOB1 cDNA全长为1734 bp,5′非编码区为466 bp,3′非编码区为296 bp,开放阅读框长972 bp,编码323个氨基酸。TOB2 cDNA全长为2785 bp,5′非编码区为51 bp,3′非编码区为1582 bp,开放阅读框为1152 bp,编码383个氨基酸。TOB1和TOB2在N端均具有明显的BTG家族结构域,且系统进化分析显示2个TOB蛋白与鲤科鱼类同源性较高,亲缘较近。qRT-PCR结果表明,TOB1和TOB2在3龄成鱼的肾脏、肠、鳃、脑、心脏、肌肉等组织中均有表达;TOB1在3龄成鱼的心脏组织中表达量最高,其次为脑和肌肉组织,在鳃组织中的表达量最低;TOB2在3龄成鱼的脑组织中表达量最高,其次为肌肉。TOB1及TOB2在不同发育时期胚胎中均有表达,且都在12 h胚胎中表达量最高。【结论】TOB1及TOB2在3龄成鱼各个组织及胚胎中均有表达,但表达量有较大差异,表明2个基因均具有时空表达特异性。

关键词： TOB基因基因克隆与表达青海湖裸鲤

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青海湖裸鲤AP-1基因的克隆与表达分析

基因组学与应用生物学 2020年第7期39卷 2964-2971页

作者：马清花陈雪妍卫唯才项卓玛梁健青海大学省部共建三江源生态与高原农牧业国家重点实验室西宁810016 青海大学生态环境工程学院西宁810016

AP-1是细胞增殖和分化的重要转录因子,在动物免疫、发育等方面发挥着重要作用。本研究采用RACE方法克隆了青海湖裸鲤AP-1基因的c DNA全长序列,通过实时荧光定量PCR探究了其在不同组织中的表达情况。结果显示,青海湖裸鲤AP-1的cDNA序列... 详细信息

AP-1是细胞增殖和分化的重要转录因子,在动物免疫、发育等方面发挥着重要作用。本研究采用RACE方法克隆了青海湖裸鲤AP-1基因的c DNA全长序列,通过实时荧光定量PCR探究了其在不同组织中的表达情况。结果显示,青海湖裸鲤AP-1的cDNA序列全长为1832 bp,CDS区918 bp,共编码305个氨基酸,预测该蛋白的分子量为33.69 kD,等电点9.20。系统进化结果显示青海湖裸鲤与犀角金线鲃有较高的同源性。蛋白结构预测显示该基因编码的蛋白C端具有一个典型的碱性区和一个高度保守的亮氨酸拉链结构,该结构能与DNA相结合。qRT-PCR结果表明,AP-1基因在肌肉、肝脏、鳃、心、肾、脑、肠和脾脏中均有表达。在肌肉中具有最高表达量(p<0.01),其次为肝脏和鳃(p<0.01),在脾脏中表达量最低。本研究为进一步了解青海湖裸鲤AP-1基因的功能提供了数据基础。

关键词： AP-1 基因克隆组织表达青海湖裸鲤

浅析清朝前期与西藏的政治互动——以《皇太极敦请萨迦法王诏书》为中心

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青海民族大学学报(社会科学版) 2016年第3期42卷 100-105页

作者：罗布才项卓玛尼玛仓觉西藏大学

《皇太极敦请萨迦法王诏书》现存于西藏博物馆,是西藏目前发现最早的清朝颁发给西藏地方的诏书。其中所册封宗教人物的考证,不仅有助于了解17世纪中叶西藏地方各种政治势力和教派力量之间的上下角逐和利益诉求,更为重要的是为研究清朝... 详细信息

《皇太极敦请萨迦法王诏书》现存于西藏博物馆,是西藏目前发现最早的清朝颁发给西藏地方的诏书。其中所册封宗教人物的考证,不仅有助于了解17世纪中叶西藏地方各种政治势力和教派力量之间的上下角逐和利益诉求,更为重要的是为研究清朝前期与西藏政治互动具有一定的参考作用。因此,笔者以此诏书的历史背景及相关人物关系着手,进一步阐析了清初西藏地方各大政治力量互相角运的社会背景。同时探讨西藏地方各政治势力为寻求清朝政治力量的支持所做出的努力,以及清朝"利用藏传佛教,笼络蒙古"治藏模式的早期实施。

关键词：清初政治互动萨迦法王诏书

Enhanced Degradation of Methyl Parathion in the Ligand Stabilized Soluble Mn(Ⅲ)-Sulfite System

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Journal of Earth Science 2019年第4期30卷 861-869页

作者： caixiang Zhang Xiaoping Liao You Lü Chao Nan State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430074China Central South China Center for Geoscience Innovation Wuhan 430074China

The ligand-stabilized soluble Mn(Ⅲ) recognized as active intermediate can potentially mediate the attenuation of contaminants. In this study,the abiotic degradation behaviors of methyl parathion in the ligand stabilized Mn(Ⅲ)-sulfite system were investigated. The results showed that the yield of soluble Mn(Ⅲ) produced from the redox reaction of MnO2 and oxalic acid was dependent linearly on the dosage of Mn O2 and caused the decomposition of methyl parathion up to 50.1% in Mn(Ⅲ)-sulfite system after 30 minutes. The fitted pseudo-first-order reaction constants of methyl parathion degradation increased with the increasing of the amount of produced Mn(Ⅲ) but was not effected linearly by the addition of sulfite. Other ligands,including pyrophosphate and oxalic acid,acted as effective complexing agents to stabilize soluble Mn(Ⅲ),and exhibited competitive effect on methyl parathion degradation with sulfite. The formation of Mn(Ⅲ)-sulfite complexes is the critical step in the system to produce abundant reactive oxygen species identified as SO3·-to facilitate methyl parathion degradation. The hydrolysis and oxidation of methyl parathion were acknowledged as two primary transformation mechanisms in Mn(Ⅲ)-sulfite system. These findings indicate that naturally ligands-stabilized soluble Mn(Ⅲ) can be generated and could oxidatively decompose organophosphate pesticides such as methyl parathion.

关键词： soluble Mn(Ⅲ) sulfite methyl parathion degradation mechanism

Induced transformation of antimony trioxide by Mn(II) oxidation and their co-transformed mechanism

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Journal of Environmental Sciences 2023年第7期129卷 69-78页

作者： You Lv caixiang Zhang Chao Nan Zenghui Fan Shuxin Huang State Key Laboratory of Biogeology and Environmental Geology China University of GeosciencesWuhan 430074China Hubei Key Laboratory of Yangtze Catchment Environmental Aquatic Science Wuhan 430074China

Antimony(Sb)is a toxic and carcinogenic element that often enters soil in the form of antimony trioxide(Sb_(2)O_(3))and coexists with manganese(Mn)in weakly alkaline *** oxides such as birnessite have been found to promote the oxidative dissolution of Sb_(2)O_(3),but few researches concerned the co-transformations of Sb_(2)O_(3) and Mn(II)in *** study investigated themutual effect of abiotic oxidation of Mn(II)and the coupled oxidative dissolution of Sb_(2)O_(3).The influencing factors,such as Mn(II)concentrations,pH and oxygen were also ***,their co-transformed mechanism was also explored based on the analysis of Mn(II)oxidation products with or without Sb_(2)O_(3) using XRD,SEM and *** results showed that the oxidative dissolution of Sb_(2)O_(3) was enhanced under higher pH and higher Mn(II)*** a lower Mn(II)concentration such as 0.01 mmol/L Mn(II)at pH 9.0,the improved dissolution of Sb_(2)O_(3) was attributed to the generation of dissolved intermediate Mn(III)species with strong oxidation ***,under higher Mn(II)concentrations,both amorphous Mn(III)oxides and intermediate Mn(III)species were responsible for promoting the oxidative dissolution of Sb_(2)O_(3).Most released Sb(∼72%)was immobilized by Mn oxides and Sb(V)was dominant in the adsorbed and dissolved total ***,the presence of Sb_(2)O_(3) not only inhibited the removal of Mn(II)by reducing Mn(III)to Mn(II)but also affected the final products of Mn *** example,amorphous Mn oxides were formed instead of crystalline Mn(III)oxides,such as ***,rhodochrosite(MnCO_(3))was formed with the high Mn(II)/Sb_(2)O_(3) ratio,but without being observed in the low Mn(II)/Sb_(2)O_(3) *** results of study could help provide more understanding about the fate of Sb in the environment and the redox transformation of Mn.

关键词： Antimony trioxide Mn(II)oxidation Oxidative dissolution Co-transformed mechanism

Ultrahigh thermal conductive graphite film via the in-situ construction of aligned nanographene skeleton using chemical vapor deposition

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Journal of Materials Science & Technology 2023年第17期148卷 1-9页

作者： Qingliang Shen Qiang Song caixiang Xiao Qiangang Fu Wei Li Shouyang Zhang Hejun Li Shaanxi Key Laboratory of Fiber Reinforced Light Composite Materials State Key Laboratory of Solidification ProcessingNorthwestern Polytechnical UniversityXi’an 710072China

Both high thermal conductivity(K)and large cross-sectional area are essential for thermal dissipation materials to maximize their heat transfer ***,the drastic decrease of K values with the increased thickness makes the existing graphite/graphene films less favored for practical *** this work,graphite film with both large thickness and high K value is produced based on an in-situ com-position strategy between nanographene(G)and pyrocarbon(PyC)via chemical vapor deposition(CVD)using CH_(3) OH/C_(2)H_(5)OH mixed ***’s found that an optimized O/C ratio of precursors facilitates the construction of ordered G skeletons within the deposited G/PyC *** G/PyC compos-ites can be completely graphitized at a lower temperature than the existing *** 2400℃ an-nealing,dense,thick,and highly aligned graphite films were *** K values reach 1350 and 1010 W m^(-1) K^(-1) at the thickness of 40 and 120μm,respectively,surpassing the existing records with similar *** importantly,the proposed method is insensitive to the deposition substrates,and the G/PyC can be infiltrated into large-size fiber preforms as a matrix for preparing centimeter-thick high K ***,the G/PyC also exhibits better mechanical and electromagnetic shielding per-formances than the existing products,indicating a promising multifunctional application prospect.

关键词： Graphene Thermal management Chemical vapor deposition

Knockdown of the atypical protein kinase genes GhABC1K2-A05 and GhABC1K12-A07 make cotton more sensitive to salt and PEG stress

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Journal of Integrative Agriculture 2024年第10期23卷 3370-3386页

作者： caixiang Wang Meili Li Dingguo Zhang Xueli Zhang Juanjuan Liu Junji Su State Key Laboratory of Aridland Crop Science/College of Life Science and Technology Gansu Agricultural UniversityLanzhou730070China Western Agricultural Research Center Chinese Academy of Agricultural SciencesChangji 831100China College of Biology and Geographical Sciences Yili Normal UniversityYining 835000China

Activity of bc1 complex kinase(ABC1K)is an atypical protein kinase(aPK)that plays a crucial role in plant mitochondrial and plastid stress responses,but little is known about the responses of ABC1Ks to stress in cotton(Gossypium spp.).Here,we identified 40 ABC1Ks in upland cotton(Gossypium hirsutum L.)and found that the Gh ABC1Ks were unevenly distributed across 17 *** GhABC1K family members included 35 paralogous gene pairs and were expanded by segmental *** GhABC1K promoter sequences contained diverse cis-acting regulatory elements relevant to hormone or stress *** qRT-PCR results revealed that most Gh ABC1Ks were upregulated by exposure to different *** ABC1K2-A05 and Gh ABC1K12-A07 expression levels were upregulated by at least three stress *** genes were further functionally characterized by virus-induced gene silencing(VIGS).Compared with the controls,the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced cotton lines exhibited higher malondialdehyde(MDA)contents,lower catalase(CAT),peroxidase(POD)and superoxide dismutase(SOD)activities and reduced chlorophyll and soluble sugar contents under NaCl and PEG *** addition,the expression levels of six stress marker genes(Gh DREB2A,Gh SOS1,Gh CIPK6,Gh SOS2,Gh WRKY33,and Gh RD29A)were significantly downregulated after stress in the Gh ABC1K2-A05-and Gh ABC1K12-A07-silenced *** results indicate that knockdown of Gh ABC1K2-A05 and Gh ABC1K12-A07 make cotton more sensitive to salt and PEG *** findings can provide valuable information for intensive studies of Gh ABC1Ks in the responses and resistance of cotton to abiotic stresses.

关键词： cotton ABC1K abiotic stress responses expression patterns virus-induced gene silencing(VIGS)

Shell Effect of Superheavy Nuclei in Self-consistent Mean-Field Models

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Communications in Theoretical Physics 2004年第6X期42卷 851-866页

作者： RENZhong-Zhou TAIFei XUChang CHENDing-Han ZHANGHu-Yong caixiang-Zhou SHENWen-Qing DepartmentofPhysics NanjingUniversityNanjing210008//ChinaCenterofTheoreticalNuclearPhysicsNationalLaboratoryofHeavy-IonAcceleratorLanzhou730000China DepartmentofPhysics NanjingUniversityNanjing210008China ShanghaiInstituteofNuclearResearch TheChineseAcademyofSciencesShanghai201800China

We analyze in detail the numerical results of superheavy nuclei in deformed relativistic mean-field model and deformed Skyrme-Hartree-Fock model. The common points and differences of both models are systematically compared and discussed. Their consequences on the stability of superheavy nuclei are explored and explained. The theoretical results are compared with new data of superheavy nuclei from GSI and from Dubna and reasonable agreement is reached. Nuclear shell effect in superheavy region is analyzed and discussed. The spherical shell effect disappears in some cases due to the appearance of deformation or superdeformation in the ground states of nuclei, where valence nucleons occupy significantly the intruder levels of nuclei. It is shown for the first time that the significant occupation of valence nucleons on the intruder states plays an important role for the ground state properties of superheavy nuclei. Nuclei are stable in the deformed or superdeformed configurations. We further point out that one cannot obtain the octupole deformation of even-even nuclei in the present relativistic mean-field model with the σ, ω and ρ mesons because there is no parity violating interaction and the conservation of parity of even-even nuclei is a basic assumption of the present relativistic mean-field model.

关键词：壳层效应超重核子变形核子物理学自相容平均场

Formation of coke in thermal cracking of jet fuel under supercritical conditions

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Frontiers of Chemical Science and Engineering 2008年第1期2卷 17-21页

作者： Yuhong ZHU caixiang YU Zimu LI Zhentao MI Xiangwen ZHANG Key Laboratory of Green Chemical Technology School of Chemical Engineering and TechnologyTianjin UniversityTianjin 300072China

Continuous-flow reactor experiments were carried out to study coke formation from thermal crack-ing of home-made jet fuel RP-3 under supercritical *** mechanism and precursor of coke forming were *** starting cracking temperature of RP-3 fuel was determined to be 471.8uC by differential scan-ning calorimetry(DSC).Temperature-programmed oxidation and scanning electron microscopy(SEM)characterizations of the stressed tubes showed that there are three different coke species including chemisorbed carbon,amorphous carbon and filamentous coke in the solid *** than 90%of coke deposits are carried away by the supercritical fluid,which has strong capabilities of extraction for coke deposits and their *** were 17.1 wt-%of iron and 11.1 wt-%of chromium found on the coke surface detected by energy dispersive spectroscopy(EDS)which suggests carbure-tion on ***-3 fuel and its cracking liquids were analyzed by GC-MS,which showed that the content of alkyl benzene and alkyl naphthalene increased evidently in cracking liquids.

关键词： coke jet fuel thermal cracking supercritical extraction