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Chromosome-Level Reference Genome and Population Genomic Analysis Provide Insights into the Evolution and Improvement of Domesticated Mulberry (Morns alba)

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Molecular Plant 2020年第7期13卷 1001-1012页

作者： Feng Jiao Rongsong Luo Xuelei Dai Hui Liu Gang Yu Shuhua Han Xin Lu Chao Su Qi Chen Qinxia Song caiting meng Fanghong Li Hongmei Sun Rui Zhang Tian Hui Yonghua Qian Aichun Zhao Yu Jiang The Sericultural and Silk Research Institute College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Key Laboratory of Animal Genetics Breeding and Reproduction of Shaanxi ProvinceCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China State Key Laboratory of Silkworm Genome Biology Institute of Sericulture and Systems BiologySouthwest UniversityChong Qing 400716China State Key Laboratory of Reproductive Regulation&Breeding of Grassland Livestock Inner Mongolia UniversityHohhot 010070China

Mulberry(Morns spp.)is the sole plant consumed by the domesticated ***,the genome of domesticated mulberry has not yet been sequenced,and the ploidy level of this species remains ***,we report a high-quality,chromosome-level domesticated mulberry(Morus alba)*** of genomic data and karyotype analyses confirmed that *** is a diploid with 28 chromosomes(2/7=2x=28).Population genomic analysis based on resequencing of 134 mulberry accessions classified domesticated mulberry into three geographical groups,namely,Taihu Basin of southeastern China(Hu mulberry),northern and southwestern China,and *** mulberry had the lowest nucleotide diversity among these accessions and demonstrated obvious signatures of selection associated with environmental *** phylogenetic analysis supports a previous proposal that multiple domesticated mulberry accessions previously classified as different species actually belong to one *** study expands our understanding of genome evolution of the genus Morus and population structure of domesticated mulberry,which would facilitate mulberry breeding and improvement.

关键词： domesticated mulberry de novo assembly population genomics comparative genomics sericulture environment adaption

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Bone analysis using an aggregation-induced emission-active iridium complex

Aggregate

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Aggregate 2023年第6期4卷 110-119页

作者： Xianpeng Zhang Xinling Liu Hongwen Yu Shuang Shen Junchao Zhi Zhen GaoJingqi Xin Jiaqi Song Liang Shao caiting meng Feifei An Taotao Huo Shichang Liu Ye Zhang Li Xu Guanying Li Department of Biophysics School of Basic Medical SciencesHealth Science CentreXi’an Jiaotong UniversityXi’anP.R.China School of Chemistry and Chemical Engineering Guangdong Pharmaceutical UniversityZhongshanP.R.China Department of Spine Surgery Honghui HospitalXi’an Jiaotong UniversityXi’anP.R.China Active Soft Matter Group Songshan Lake Materials LaboratoryDongguanP.R.China School of Public Health Health Science CentreXi’an Jiaotong UniversityXi’anP.R.China

Fluorescent analysis of bone provides valuable insights into bone ***,conventional dyes suffer from low specificity on bone tissue,small stokes shift,short fluorescent lifetime,and aggregation-caused quenching effect,which result in low efficacy and *** this work,we design an aggregation-induced emission(AIE)-active iridium(III)complex(Ir-BP2)as a highly selective,convenient,nondestructiveness,and dual-mode staining agent for bone ***-BP2 containing phosphonate groups selectively binds to hydroxyapatites,the main component of bone matrix,and exhibits turn-on AIE phosphorescence with prolonged ***-BP2 exhibits promising biosafety and offers higher accuracy in staining calcium deposits than conventional Alizarin Red S staining assay when it is employed in real-time monitoring of osteogenesis differentiation process.A ready-to-use staining spray of Ir-BP2 is *** using fluorescent imaging and lifetime imaging,Ir-BP2 staining provides valuable insights into bone microstructure analysis,microdamage diagnosis,and bone growth state ***,Ir-BP2 is successfully applied on a human spine vertebra for diagnosing bone invasiveness of eosinophilic granuloma,validating its clinical *** work presents a powerful tool in bone analysis and will lead to new approaches for the diagnosis and treatment of bone-related diseases.

关键词： aggregation-induced emission bone analysis clinical diagnosis eosinophilic granuloma iridium complexes phosphorescent probe

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan Wen Zhigang Tao Hai Pu Tianci Deng Yaoyao meng Qingbin meng Hongwen Jing Bo meng Jiangyu Wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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桑树谷胱甘肽还原酶基因的克隆及其对非生物胁迫的响应

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蚕业科学 2020年第4期46卷 397-405页

作者：孟彩婷余刚陆鑫钱永华苏超焦锋西北农林科技大学蚕桑丝绸研究所陕西杨凌712100

谷胱甘肽还原酶(glutathione reductase,GR,EC 1.***.4.2)作为抗坏血酸-谷胱甘肽(AsA-GSH)循环中一种重要的氧化还原酶类,广泛参与植物调控生长、发育和响应环境胁迫过程。目前,桑树(Morus L.)GR基因的序列尚未克隆得到,而其表达模式也未... 详细信息

谷胱甘肽还原酶(glutathione reductase,GR,EC 1.***.4.2)作为抗坏血酸-谷胱甘肽(AsA-GSH)循环中一种重要的氧化还原酶类,广泛参与植物调控生长、发育和响应环境胁迫过程。目前,桑树(Morus L.)GR基因的序列尚未克隆得到,而其表达模式也未见报道。本研究利用RT-PCR技术从桑树品种红果2号中克隆到2个GR基因,分别命名为MaGR1和MaGR2,并对其进行了生物信息学、亚细胞定位和时空表达分析。结果表明,MaGR的氨基酸序列包含GR蛋白家族的典型结构域,包括GSSG和NADPH结合位点等;MaGR1和MaGR2分别属于胞质亚型组和叶绿体亚型组;桑树MaGR与拟南芥AtGR基因结构相似,但不完全相同;亚细胞定位显示MaGR1蛋白定位于细胞质、细胞膜和细胞核,MaGR2蛋白主要位于叶绿体;实时荧光定量qRT-PCR分析结果表明,MaGR1和MaGR2在根、茎、叶芽、叶片、花和幼果中均表达,且在叶芽和叶片中存在高表达。MaGR对干旱、高低温以及氧化胁迫均有不同程度的响应,随着处理时间的增加而普遍呈现先上升后下降的趋势,暗示其在桑树响应非生物胁迫中可能发挥重要作用。该研究为进一步探索MaGR基因在桑树抗逆反应中的调控作用奠定了基础。

关键词：谷胱甘肽还原酶桑树基因克隆亚细胞定位表达分析

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不同培养基对桑树茎段外植体摄取6-BA和增殖生长的影响

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蚕业科学 2021年第4期47卷 301-309页

作者：陆鑫孟彩婷韩淑华钱永华李芳红焦锋西北农林科技大学蚕桑丝绸研究所陕西杨凌712100 西北农林科技大学动物科技学院陕西杨凌712100

为优化桑树外植体组织培养体系,以添加质量浓度0~4.0 mg/L的6-BA为条件,调查印度桑品种K2(Morus indica cv. K2)在B5、DKW、MS和WPM培养基中外植体继代培养的增殖形态、生理指标与外植体内6-BA浓度的相关性。结果表明,外植体在WPM培养... 详细信息

为优化桑树外植体组织培养体系,以添加质量浓度0~4.0 mg/L的6-BA为条件,调查印度桑品种K2(Morus indica cv. K2)在B5、DKW、MS和WPM培养基中外植体继代培养的增殖形态、生理指标与外植体内6-BA浓度的相关性。结果表明,外植体在WPM培养基添加0.2 mg/L IBA和2.0 mg/L 6-BA时增殖效果最好,30 d时质量增殖倍数最大值为9.32,芽净增殖数最大值为24.67;可溶性蛋白质量分数为3.146%(m_(f)),叶绿素质量分数为0.359 mg/g(m_(f));外植体内6-BA质量浓度最大值达到46.053 ng/L,显著高于其他培养基中外植体内的浓度。由此表明,WPM培养基促进桑树外植体对外源6-BA的吸收,进而使外植体内可溶性蛋白和叶绿素维持相对较高水平,促进了生长发育。本研究为探索桑树组织培养的生长调控机制提供了一种新的研究思路。

关键词：桑树培养基外植体 6-BA 继代增殖生理指标

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桑树MaGPX家族基因的克隆及表达分析

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西北植物学报 2019年第6期39卷 991-1000页

作者：李帅军张敏娟孟彩婷苏超董小龙陈坤明李文强西北农林科技大学生命科学学院陕西杨陵712100 西北农林科技大学蚕桑丝绸研究所陕西杨陵712100

谷胱甘肽过氧化物酶(Glutathione peroxidases,GPXs)是植物细胞清除活性氧的重要酶类之一,与植物的抗逆性紧密相关。该研究以桑树(Morus alba L.)‘红果2号’为材料,利用RT-PCR方法克隆了桑树MaGPX基因家族6个成员。生物信息学分析表明,... 详细信息

谷胱甘肽过氧化物酶(Glutathione peroxidases,GPXs)是植物细胞清除活性氧的重要酶类之一,与植物的抗逆性紧密相关。该研究以桑树(Morus alba L.)‘红果2号’为材料,利用RT-PCR方法克隆了桑树MaGPX基因家族6个成员。生物信息学分析表明,MaGPX蛋白序列具有植物GPX的典型结构域和Cys残基,并与拟南芥(Arabidopsis thaliana)AtGPX蛋白序列具有较高的相似性。亚细胞定位结果表明,MaGPX1/2/3/6可能定位在细胞膜、细胞质和细胞核,MaGPX4定位在叶绿体,MaGPX5可能定位在细胞膜,暗示桑树MaGPX成员具有功能的分化。qRT-PCR分析表明,MaGPX基因家族各成员在芽、叶、根、雄花、雌花以及果实中均有表达,其中MaGPX 1/3/5在根以及雄花中高表达,MaGPX 2在雄花中高表达,MaGPX 4在叶和雄花中高表达,MaGPX 6在果实中高表达;MaGPX各成员的表达受干旱胁迫诱导,各成员对干旱胁迫的响应随着处理时间和胁迫程度的变化而有所不同,其中MaGPX 1、MaGPX 2和MaGPX 3可能在清除活性氧和维持细胞内氧化还原平衡中发挥重要作用。该研究结果为桑树MaGPX基因家族的生理功能研究奠定了良好基础。

关键词：谷胱甘肽过氧化物酶桑树克隆亚细胞定位干旱胁迫

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桑叶酶解产物的制备及其抗氧化活性评价

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蚕业科学 2020年第4期46卷 485-492页

作者：余刚邱茂慈孟彩婷陆鑫韩淑华宁军权李芳红焦锋西北农林科技大学蚕桑丝绸研究所陕西杨凌712100 陕西省平利县农业技术推广站陕西安康725500

为充分利用桑叶蛋白质资源,在单因素试验的基础上结合响应面法优化酶解制备桑叶多肽的工艺条件,以化学抗氧化能力评价其抗氧化性能。结果表明,以中性蛋白酶为酶源,在底物质量分数2.58%、酶添加量1 000 U/g、酶解时间5 h、酶解温度39.16... 详细信息

为充分利用桑叶蛋白质资源,在单因素试验的基础上结合响应面法优化酶解制备桑叶多肽的工艺条件,以化学抗氧化能力评价其抗氧化性能。结果表明,以中性蛋白酶为酶源,在底物质量分数2.58%、酶添加量1 000 U/g、酶解时间5 h、酶解温度39.16℃、pH 7.56时,桑叶蛋白的酶解效果最佳,此条件下水解度可达20.26%。在该条件下DPPH清除能力、羟自由基清除能力、亚铁离子螯合能力的EC50分别为6.795、0.073和0.374 mg/mL。结果表明用桑叶能够用于制备抗氧化性能良好的多肽。

关键词：桑叶酶解工艺肽抗氧化活性

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The autophagy-lysosome pathway:a potential target in the chemical and gene therapeutic strategies for Parkinson’s disease

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Neural Regeneration Research 2025年第1期20卷 139-158页

作者： Fengjuan Jiao Lingyan meng Kang Du Xuezhi Li School of Mental Health Jining Medical UniversityJiningShandong ProvinceChina Shandong Collaborative Innovation Center for Diagnosis Treatment and Behavioral Interventions of Mental DisordersInstitute of Mental HealthJining Medical UniversityJiningShandong ProvinceChina

Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in *** one of the major intracellular degradation pathways,the autophagy-lysosome pathway plays an important role in eliminating these *** evidence has shown that upregulation of the autophagy-lysosome pathway may contribute to the clearance ofα-synuclein aggregates and protect against degeneration of dopaminergic neurons in Parkinson’s ***,multiple genes associated with the pathogenesis of Parkinson’s disease are intimately linked to alterations in the autophagy-lysosome ***,this pathway appears to be a promising therapeutic target for treatment of Parkinson’s *** this review,we briefly introduce the machinery of ***,we provide a description of the effects of Parkinson’s disease–related genes on the autophagy-lysosome ***,we highlight the potential chemical and genetic therapeutic strategies targeting the autophagy–lysosome pathway and their applications in Parkinson’s disease.

关键词： autophagy chemical therapy gene therapy Parkinson’s disease α-synuclein

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A Novel Model for Describing Rail Weld Irregularities and Predicting Wheel-Rail Forces Using a Machine Learning Approach

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Computer Modeling in Engineering & Sciences 2025年第1期142卷 555-577页

作者： Linlin Sun Zihui Wang Shukun Cui Ziquan Yan Weiping Hu Qingchun meng State Key Laboratory of High-Speed Railway Track System China Academy of Railway Sciences Corporation LimitedBeijing100081China Railway Engineering Research Institute China Academy of Railway Sciences Corporation LimitedBeijing100081China School of Aeronautic Science and Engineering Beihang UniversityBeijing100191China

Rail weld irregularities are one of the primary excitation sources for vehicle-track interaction dynamics in modern high-speed *** can cause significant wheel-rail dynamic interactions,leading to wheel-rail noise,component damage,and *** researchers have employed the vehicle-track interaction dynamic model to study the dynamic interactions between wheel and rail induced by rail weld geometry ***,the cosine wave model used to simulate rail weld irregularities mainly focuses on the maximum value and neglects the geometric *** this study,novel theoretical models were developed for three categories of rail weld irregularities,based on measurements of the high-speed railway from Beijing to *** vertical dynamic forces in the time and frequency domains were compared under different running *** forces generated by the rail weld irregularities that were measured and modeled,respectively,were compared to validate the accuracy of the proposed ***,based on the numerical study,the impact force due to rail weld irrregularity is modeled using an Artificial Neural Network(ANN),and the optimum combination of parameters for this model is *** results showed that the proposed model provided a more accurate wheel/rail dynamic evaluation caused by rail weld irregularities than that established in the *** ANN model used in this paper can effectively predict the impact force due to rail weld irrregularity while reducing the computation time.

关键词： Rail weld irregularity high-speed railway vehicle-track coupled dynamics wheel/rail dynamic vertical force artificial neural networks

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Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： Jing Li Zixuan He Weitai Chai meng Tian Huali Yu Xiaoxiao He Xiaojuan Zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan

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