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Optimization of the Fermentation Medium for Alpha-Galactosidase Production from Aspergillus foetidus ZU-G1 Using Response Surface Methodology

Optimization of the Fermentation Medium for Alpha-Galactosid...

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饲用酶制剂开发与应用技术交流研讨会

作者： caiquin liu Dept.of Food Science and Nutrition Zhejiang Univ.

The optimization of fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources,nitrogen sources,and essential elements) for alpha-galactosidase production. A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KHPO,FeSO·7HO, and the medium initial pH,that affected the production of *** central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation medium. The experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-G1 was composed of 3.2%soybean meal(w/v),2%wheat bran(w/v),0.1%KHPO(w/v), and 0.05%FeSO·7HO(w/v);initial medium pH was *** results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 96-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized *** time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate themodel.

关键词： alpha-galactosidase Aspergillus foetidus ZU- G1 crude sources medium optimization response surface methodology

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Optimization of the Fermentation Medium for Alpha-Galactosidase Production from Aspergiilus foetidus ZU-G1 Using Response Surface Methodology

Optimization of the Fermentation Medium for Alpha-Galactosid...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： caiquin liu Hui RUAN Huafeng SHEN Qihe CHEN Bing ZHOU Yi LI AND Guoqing HE (Authors liu,Ruan,Shen,Chen,Zhou,and He are with Dept.of Food Science Nutrition,Zhejiang Univ.,Hangzhou,310029,China.Author Li is with Dept.of PlantSciences,NorthDakota StateUniv.,Fargo,ND58105,U.S.A.)

The optimization of fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-Gl was investigated in shaker flask fermentation.A one-factor-at-a-time experiment was used to screen the preferable nutriment(carbon sources, nitrogensources,andessential elements)for alpha-galactosidase production.A fractional factorial design was used to screen the main 5 factors,soybean meal,wheat bran,KH2PO4,FeSO4·7H2O, and the medium initial pH,that affected the production of *** central composite experimental design was further adopted to derive a statistical model for optimizing the composition of the fermentation *** experimental results showed that the optimum fermentation medium for alpha-galactosidase production by Aspergillus foetidus ZU-Gl was composed of 3.2% soybean meal(w/v),2% wheat bran(w/v),0.1% KH2PO4(w/v),and 0.05% FeSO4.7H20(w/v);initial medium pH was *** results further predicted that alpha-galactosidase activity reached 64.75 U/mL after 95-h incubation in this medium,which was approximately 7 times higher than that incubated in the nonoptimized *** time course of alpha-galactosidase production in the optimized medium composition was also carried out to validate the model.

关键词： alpha-galactosidase Aspergillus foetidus ZU-G1 crude sources medium optimization response surface Methodology

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