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Influence of Concentrate in the Diet on the Metabolic, Biochemical, and Endocrine Profile of Buffalo Heifers

Open Journal of Veterinary Medicine 2019年第7期9卷 79-90页

作者： caio alves da costa Rafael Artur da Silva Júnior Bruna Higino de Souza Silva Rebeka Pontes Menezes Ayna Arramis Apolinário da Silva Felipe Rosendo Correia Emanuel Felipe de Oliveira Filho Ricardo Alexandre Silva Pessoa Cláudio Coutinho Bartolomeu Pierre Castro Soares Department of Veterinary Medicine Federal Rural university of Pernambuco Recife Brazil Department of Animal Science Federal Rural university of Pernambuco Recife Brazil

The objective of this study was to evaluate the profile of the main serum biochemical variables in buffalo heifers receiving diets with different concentrations of concentrate. Twenty buffalo heifers of the Murrah breed, aged six to eight months, were randomly assigned to four groups, with five replicates each, to be adapted to levels of 20%, 40%, 60%, and 80% of concentrate in the diet, composed of sugarcane (bulk) and concentrated feed based on wheat, corn, and soybean meal. The experimental diets were constituted in the proportion “bulk: concentrate”, based on dry matter, of “80:20” (Treatment 1), “60:40” (Treatment 2), “40:60” (Treatment 3), and “20:80” (Treatment 4). Biomarkers were evaluated for energy, protein, mineral, and endocrine profiles, as well as enzymatic activity. A positive linear effect was observed for Final Body Weight. In the energy profile, there was a positive linear effect for total cholesterol and HDL cholesterol and a quadratic effect for plasma glucose. In the protein profile, a quadratic effect was observed for the urea: creatinine ratio and a positive linear effect for total protein and albumin. For the enzymatic activity, only the variable gamma-glutamyltransferase presented a quadratic effect. In the mineral profile, a quadratic effect was observed for calcium levels and a positive linear effect for phosphorus levels and negative for chlorine levels. In the endocrine profile, no statistical differences were observed. Diet with until 80% of concentrate does not negatively influence biochemical parameters that represent the profile of energy, protein, mineral and thyroid hormones for growing buffalo heifers and favors the performance of the animals.

关键词： Animal Nutrition Clinical Pathology Bubalus Bubalis Energy Profile

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The Effect of High Carbohydrate Consumption on Glucose Levels and Antibody Production in Nonobese Diabetic Mice

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Food and Nutrition Sciences 2016年第10期7卷 866-873页

作者： Tonicley Alexandre da Silva caio José de Carvalho-Filho Elizabeth de Sousa Barcelos Barroqueiro Deysianne costa das Chagas Flá via Raquel Fernandes Nascimento Rosane Nassar Meireles Guerra Laboratory of Imunophysiology Universidade Federal do Maranh&atilde o S&atilde o Luí s Brazil

The aim of this study was to evaluate the effect of chronic treatment with diets rich in carbohydrates on the IgM and IgG antibody production and the seric glucose concentration in diabetes. Nonobese diabetic (NOD) mice received, ad libitum, by oral route, the diet consisting of an aqueous extract (20 mg/mL) of the following flours: babassu mesocarp, manioc, corn or rice, during 120 days. The diet intake was monitored throughout this period. At the end, the weight variation, blood glucose, serum IgG and IgM antibody and IgM anti-insulin titers, were determined. The babassu and manioc flour extracts altered Purina chow intake and these animals also presented a significant increase in body weight. In contrast, treatment with rice flour resulted in a significant weight loss. Moderate to severe hyperglycemia was observed in the groups receiving rice and manioc, whereas treatment with babassu mesocarp flour and cornmeal resulted in hypoglycemia. The extracts did not alter the IgG concentration. On the other hand, the cornmeal extract caused a marked reduction in both total IgM and anti-insulin IgM antibody production. Although babassu mesocarp flour, cornmeal and manioc flour caused important variations in the parameters studied, only treatment with the rice flour extract anticipated the onset of diabetes in male mice genetically predisposed to the disease.

关键词： Carbohydrates Diabetes Flour NOD Mice Diet

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Gastric Gist as Cause of High Digestive Hemorrhage

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Journal of Biosciences and Medicines 2019年第1期7卷 42-47页

作者： Vinicius Moreira Paladino Thiago Sande Miguel Vinicius Sande Miguel Marcia Machado Galvao Pereira Fernando Antonio Galvao Pereira Carolina Galvao Teixeira Caroline Guida Babinski Nayrton Kalys Cruz dos Anjos caio Marcus de Andrade Lopes daniel Almeida da costa The Medical School at the Center of Higher Education of Valenca ValencaBrazil Unigranrio Medical School Rio de JaneiroBrazil Faculty of Medical Sciences and Health of Juiz de Fora Juiz de ForaBrazil

Introduction: Gastrointestinal stromal tumor (GIST) occurrence is uncommon and usually manifested by nonspecific signs and symptoms, which hinders its reasoning as a diagnostic hypothesis. The objective is to report a case of female GIST with curative treatment through video-laparoscopy with partial gastrectomy. Case report: A 61-year-old female patient attended the Luiz Gioseffi Jannuzzi School Hospital (HELGJ) emergency room, with a sudden onset of upper gastrointestinal bleeding with hemodynamic instability. After stabilization, she was admitted for diagnostic investigation, showing sub-epithelial lesion of antrum in upper digestive endoscopy (UDE) and solid-cystic mass in the gastric antrum to computed tomography (CT). The conduct was the video laparoscopy surgical approach with partial gastrectomy. The histopathological and immunohistochemical aspects of the surgical specimen showed a gastrointestinal stromal tumor (GIST) in a gastric antrum of low grade of malignancy, positive e-Kit and mycotic index (50 cga) Discussion: Compared to the scarce reports in literature, the case described presented typical epidemiology, but the clinical picture was not the most incident. Conclusions: Given the rarity of this pathology cases, it is essential to report these in order to elucidate the specificities and also to discuss diagnostic and therapeutic methods.

关键词： Gastrointestinal Stromal Tumor Gastrointestinal Hemorrhage Immunohistochemistry

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Human Seroreactivity to Secreted Molecules of Corynebacterium pseudotuberculosis

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Advances in Microbiology 2022年第3期12卷 150-158页

作者： Ramon Mendes dos Santos Silvânia Maria Andrade Cerqueira caio Lopez Borge Andrade Gabriel Saldanha Müller Vitória costa de Menezes Santos Hévlyn Ribeiro de Araújo Samanta Queiroz Rogério Reis Conceição Luiz Gustavo Freitas Oliveira Marcos Borges Ribeiro Silvana Marchioro Lilia Ferreira de Moura-costa Fúlvia Soares Campos de Sousa Maria da Conceição Aquino de Sá José Tadeu Raynal Rocha Filho Soraya Castro Trindade Eduardo Martins Netto Roberto Meyer Songelí Menezes Freire Laboratory of Immunology and Molecular Biology of the Health Sciences Institute of the Federal University of Bahia Bahia Brazil Specialized Hospital Otávio Mangabeira Bahia Brazil Microbiology of the Federal University of Bahia Bahia Brazil Laboratory of Infectology of the University Hospital Complex Professor Edgard Santos Salvador Bahia Brazil

Corynebacterium pseudotuberculosis is an infectious agent that occurs in small ruminants causing caseous lymphadenitis, and more rarely in humans causing lymphadenitis and pneumonia. The breeding small ruminants have great economic importance in Brazil. Rural farm workers and veterinary students who acquired this disease suffered from weakening symptoms for weeks, and the identification of the etiological agent was time-consuming and complex. Due to the low prevalence of case records, there is probably no available commercial diagnostic kit for C. pseudotuberculosis infection in humans. This study aimed to describe human seroreactivity to secreted antigens from C. pseudotuberculosis. Reactivity of serum from farm workers (n = 14), individuals who work with the bacillus at laboratory (n = 8) or individuals without contact (n = 25) was tested with secreted proteins from PAT10 strain of C. pseudotuberculosis by Western blotting. Samples of all (100%) farm workers showed reactivity to 31 kda, 71 kda and 164 kda proteins, while laboratory workers showed 87.5%, 62.5 % and 37.5%, and no-contact 20%, 0% and 16%, respectively. All sera recognized the 275 kda protein. Our data suggest that C. pseudotuberculosis secreted proteins are antigenic in humans and the recognition profiles allowed the identification of individuals with and without prior contact with this bacillus. This is the first paper which describes human reactivity to C. pseudotuberculosis in serum samples of workers in Brazil.

关键词： Human Immunoreactivity Antigenicity Corynebacterium pseudotuberculosis Secreted Proteins Western Blot

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