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Chinese Science Bulletin 2010年第27期55卷 3154-3160页

作者： ZHANG YuShan WANG JianBo xu caiguo XING YongZhong National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 China University of Electronic Science and Technology of China Zhongshan College Zhongshan 528402 China

To enhance understanding of the genetic basis of trait correlation in rice,a recombinant inbred line(RIL)population(F6 and F7) from a cross between Zhenshan97 and HR5 was employed to identify main quantitative trait loci(QTLs)and epistatic QTL(E-QTL).Highly significant positive correlations were detected among five traits of heading date(HD),plant height(PH),panicle length(PL),flag leaf length(FLL)and flag leaf width(FLW)in 2 *** to 8 main QTLs were detected for an individual *** E-QTL was detected for ***,4,4 and 5 E-QTLs were detected for FLL,HD,FLW and PL,*** E-QTL individually explained less than 3%of trait variation except *** of QTL results was made in order to dissect the genetic basis of trait *** found that main QTLs with pleiotropic effects and QTL clusters were the main genetic basis of trait *** E-QTL had pleiotropic effects.E-QTL played an important role in the genetic basis of individual trait,but it made a little contribution to trait correlation.

关键词：形态性状遗传基础 Oryza 水稻解剖 QTL检测 QTLs 电子邮件

Comparative analysis of gene expression at early seedling stage between a rice hybrid and its parents using a cDNA microarray of 9198 uni-sequences

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Science China(Life Sciences) 2006年第6期49卷 519-529页

作者： HUANG Yi LI Lihua CHEN Ying LI Xianghua xu caiguo WANG Shiping ZHANG Qifa National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research WuhanHuazhong Agricultural UniversityWuhan 430070China

Using a cDNA microarray consisting of 9198 expressed sequence tags,we surveyed the gene expression profiles in shoots and roots of a rice hybrid,Liangyoupei 9 and its parents Peiai 64s and 93-11 at 72 h after germination.A total of 8587 sequences had detectable signals in both shoots and roots of the three genotypes.A total of 1571 sequences exhibited significant(P<0.01)expression differences in shoots or roots among the three genotypes,of which 121 showed expression poly-morphisms in both shoots and roots,and 870 revealed significant expression differences between the hybrid and one of the *** expression polymorphism of the sequences was associated with the functional categories of the *** occurred more frequently in categories of carbohy-drate,energy and lipid metabolisms and stress response than expected,while less frequently in categories of amino acid metabolism,transcription and translation regulation,and signal transduction.A total of 214 sequences exhibited significant(P<0.05)mid-parent heterosis in expression,of which 117 had homology to genes with known functions,assigned in the categories of basic metabolism,genetic information processing,cell growth and death,signal transduction,transportation and stress *** results may provide useful information for exploring the relationship between gene expression polymorphism and phenotypic variation,and for characterizing the molecular mechanism of seedling development and heterosis in rice.

关键词： Oryza sativa shoots roots gene expression microarray heterosis

Genetic Components of Heterosis for Seedling Traits in an Elite Rice Hybrid Analyzed Using an Immortalized F2 Population

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Journal of Genetics and Genomics 2016年第2期43卷 87-97页

作者： Dan Zhu Gang Zhou caiguo xu Qifa Zhang National Key Laboratory of Crop Genetic Improvement National Center of Plant Gene Research(Wuhan)Huazhong Agricultural UniversityWtthzm 430070China

Utilization of heterosis has greatly contributed to rice productivity in China and many Asian countries. Superior hybrids usually show heterosis at two stages： canopy development at vegetative stage and panicle development at reproductive stage resulting in heterosis in yield. Although the genetic basis of heterosis in rice has been extensively investigated, all the previous studies focused on yield traits at maturity stage. In this study, we analyzed the genetic basis of heterosis at seedling stage making use of an ＂immortalized F2＂ population composed of 105 hybrids produced by intercrossing recombinant inbred lines （RILs） from a cross between Zhenshan 97 and Minghui 63, the parents of Shanyou 63, which is an elite hybrid widely grown in China. Eight seedling traits, seedling height, tiller number, leaf number, root number, maximum root length, root dry weight, shoot dry weight and total dry weight, were investigated using hydroponic culture. We analyzed single-locus and digenic genetic effects at the whole genome level using an ultrahigh-density SNP bin map obtained by population re-sequencing. The analysis revealed large numbers of heterotic effects for seedling traits including dominance, over- dominance and digenic dominance （epistasis） in both positive and negative directions. Overdominance effects were prevalent for all the traits, and digenic dominance effects also accounted for a large portion of the genetic effects. The results suggested that cumulative small advantages of the single-locus effects and two-locus interactions, most of which could not be detected statistically, could explain the genetic basis of seedling heterosis of the F1 hybrid.

关键词： Heterosis Seedling trait Immortalized F2 Overdominance Digenic dominance

Additive and additive × additive interaction make important contributions to spikelets per panicle in rice near isogenic (Oryza sativa L.) lines

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Journal of Genetics and Genomics 2010年第12期37卷 795-803页

作者： Qin He Kexin Zhang caiguo xu Yongzhong Xing National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China

Epistasis plays an important role in the genetic basis of rice yield traits. Taking interactions into account in breeding programs will help the development of high-yielding rice varieties. In this study, three sets of near isogenic lines （NILs） targeting three QTLs for spikelets per panicle （SPP）, namely qSPP1, qSPP2 and qSPP7, which share the same Zhenshan 97 genetic background, were used to produce an F 2 population in which the three QTLs segregated simultaneously. The genotypes of the individual F 2 plants at the three QTLs were replaced with three markers that are closely linked to the corresponding QTLs. These QTLs were validated in the F 2 and F 3 populations at the single marker level. qSPP7 exhibited major pleiotropic effects on SPP, plant height and heading date. Multifactor analysis of variance was performed for the F 2 population and its progeny. Additive × additive interaction between qSPP2 and qSPP7 had significant effects on SPP in both the F 2 population and its progeny. Both additive and additive × additive interactions could explain about 73% of the total SPP phenotypic variance. The SPP performance of 27 three-locus combinations was ranked and favorable combinations were rec- ommended for rice breeding in different ecosystems.

关键词： QTL validation phenotypic variance pleiotropic effect favorable combination

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基于知识创新和转化的高校企业研究

科技管理研究 2005年第6期25卷 109-111页

作者：卢锐许彩国南京大学商学院江苏南京210093 南京审计学院管理系江苏南京210029

在推进高校科研成果商品化、产业化和国际化工作中,高校企业的知识创新和转化问题显得十分重要。本文从知识创新和转化的一般过程的角度研究高校企业的发展,并针对我国的现状提出若干建议。

关键词：高校企业创新转化

Complete Dissection of the Genetic Basis of Heterosis in a Highly Heterotic Rice Hybrid Based on a Ultra-high Density Linkage Map

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Complete Dissection of the Genetic Basis of Heterosis in a H...

Molecular regulation of bract suppression during panicle development in rice

2010全国植物生物学研讨会

作者： Gang Zhou Weibo Xie Yongzhong Xing caiguo xu Qifa Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural University

We have been investigating genetic and molecular bases of yield and heterosis in rice using a combination of approaches,with the ultimate goal to achieve a comprehensive understanding of the biology of yield and *** focused the effort in yield traits of Shanyou 63,a cross between Zhenshan 97 and Minghui 63,which is the most widely

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Molecular regulation of bract suppression during panicle dev...

2016年全国植物生物学大会

作者： Lei Wang Shengyuan Sun caiguo xu Xianghua Li Jinghua Xiao Qifa Zhang National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural University

The reproductive transition in rice is characterized by a number of dramatic changes. Two of the most striking features of these changes are the suppression of bract,and the change of the lateral organ identity from tiller branching into panicle branching. Both of these features indicate suppression of the vegetative programs for panicle development. However,the underlying mechanisms are not well known. We identified a mutant panicle with ectopic leaf(pel) that failed to suppress bract outgrowth. PEL had two paralogs in rice known as PEL-like 1 and 2(PEL1,2). All three PELs were expressed in the bract primordium revealed by in situ hybridization,and PEL proteins formed homodimers and heterodimers in vitro and in vivo. We used CRISPR/Cas9 to generate single gene and triple-gene mutants. Much more bracts were formed in the triple mutant pel pel1 pel2 than any single gene mutant. Moreover,the panicle branches were also completely supplanted by the vegetative shoots in the pel pel1 pel2 plants. Therefore,PELs redundantly terminated the vegetative programs after reproductive transition. Y2 H was used to screen the interacting proteins of PELs,and one protein PEL Interacted Protein 1(PIP1) was identified. Pull-down,Bi FC and LCI further substantiated the physical interactions between PIP1 and PELs in vitro and in planta. Moreover,PIP1 was co-expressed with the PELs based on the global transcriptomic analysis,suggesting that PELs might regulate PIP1 or vice versa. The results of Y1 H,EMSA and Ch IP-q PCR demonstrated that PELs directly regulated PIP1 in rice. Genetic analysis showed that PIP1 also repressed bract outgrowth. All of these genes encoded transcription factors,suggesting that they should have vital roles in reprogramming the transcriptomes following the reproductive transition. Several genomic approaches will be used to address this question,and the related works are underway. According to these results,a complex network of genes required for terminating the ve

关键词： transcription factor reproductive transition inflorescence architecture

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试论核心竞争力与中小企业发展

生产力研究 2007年第7期 127-129页

作者：许彩国李振宇南京审计学院江苏南京210029

文章试图从中小企业的自身特点出发,以核心竞争力为切入点,探究中小企业保持持续健康发展的动力源泉,在此基础上进一步分析核心竞争力培育的基本思路和策略。以此来说明:中小企业只有积极培育出核心竞争力,保持鲜明的企业个性,才能创造... 详细信息

文章试图从中小企业的自身特点出发,以核心竞争力为切入点,探究中小企业保持持续健康发展的动力源泉,在此基础上进一步分析核心竞争力培育的基本思路和策略。以此来说明:中小企业只有积极培育出核心竞争力,保持鲜明的企业个性,才能创造出持续的竞争优势,在激烈的市场竞争中获得生存与发展。

关键词：核心竞争力中小企业

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服装大规模定制产品开发模式的现状与对策

纺织学报 2009年第10期30卷 143-148页

作者：许才国刘晓刚东华大学上海200051 宁波大学浙江宁波315211

针对当前服装大规模定制企业在产品开发环节存在的不足之处,构建了面向服装大规模定制产品开发的智能设计系统,通过对系统中大规模定制服装款式配置设计和快速变型设计的工作原理,以及功能模拟界面的分析,说明该系统在服装大规模定制企... 详细信息

针对当前服装大规模定制企业在产品开发环节存在的不足之处,构建了面向服装大规模定制产品开发的智能设计系统,通过对系统中大规模定制服装款式配置设计和快速变型设计的工作原理,以及功能模拟界面的分析,说明该系统在服装大规模定制企业产品开发环节的应用中具有便于顾客参与定制产品设计、快速生成定制产品效果图、准确定义产品、快速反应客户订单等方面的优点,能够充分体现服装大规模定制客户化设计与制造的竞争优势。

关键词：服装大规模定制产品开发现有模式应对策略智能设计

An eQTL guided co-expression analysis for constructing regulatory network using a rice RIL population

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An eQTL guided co-expression analysis for constructing regul...