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Dietary supplementation of benzoic acid and essential oils combination enhances intestinal resilience against LPS stimulation in weaned piglets

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Journal of Animal Science and Biotechnology 2024年第2期15卷 878-897页

作者： Chang Cui Yulong Wei Yibo Wang Wen Ma Xiaoyu Zheng Jun Wang Ziwei Ma caichi wu Licui Chu Shihai Zhang wutai Guan Fang Chen Guangdong Provincial Key Laboratory of Animal Nutrition Control College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhou 510642China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642China

Background The benefits of combining benzoic acid and essential oils(BAO)to mitigate intestinal impairment during the weaning process have been well established,while the detailed underlying mechanism has not been fully elucidated.Previous research has primarily focused on the reparative effects of BAO on intestinal injury,while neglecting its potential in enhancing intestinal stress resistance.Methods In this study,we investigated the pre-protective effect of BAO against LPS-induced stress using a modified experimental procedure.Piglets were pre-supplemented with BAO for 14 d,followed by a challenge with LPS or saline to collect blood and intestinal samples.Results Our findings demonstrated that BAO supplementation led to significant improvements in piglets’final weight,average daily gain,and feed intake/body gain ratio.Additionally,BAO supplementation positively influenced the composition of intestinal microbiota,increasing beneficial Actinobacteriota and Alloprevotella while reducing harmful Desulfobacterota,Prevotella and Oscillospira.Furthermore,BAO supplementation effectively mitigated oxidative disturbances and inflammatory responses induced by acute LPS challenge.This was evidenced by elevated levels of T-AOC,SOD,and GSH,as well as decreased levels of MDA,TNF-α,and IL-6 in the plasma.Moreover,piglets subjected to LPS challenge and pre-supplemented with BAO exhibited significant improvements in intestinal morphological structure and enhanced integrity,as indicated by restored expression levels of Occludin and Claudin-1 compared to the non-supplemented counterparts.Further analysis revealed that BAO supplementation enhanced the jejunal antioxidative capacity by increasing GSH-Px levels and decreasing MDA levels under the LPS challenge and stimulated the activation of the Nrf2 signaling pathway.Additionally,the reduction of TLR4/NF-κB/MAPK signaling pathways activation and proinflammatory factor were also observed in the jejunal of those piglets fed with BAO.Conclusions In summary,our study demonstrates that

关键词： Anti-stress Benzoic acid Essential oils Intestine LPS Weaned piglets

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Restored intestinal integrity,nutrients transporters,energy metabolism,antioxidative capacity and decreased harmful microbiota were associated with IUGR piglet's catch-up growth before weanling

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Journal of Animal Science and Biotechnology 2023年第1期14卷 390-408页

作者： Chang Cui caichi wu Jun Wang Ziwei Ma Xiaoyu Zheng Pengwei Zhu Nuan Wang Yuhua Zhu wutai Guan Fang Chen Guangdong Provincial Key Laboratory of Animal Nutrition Control College of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642China. Shenzhen Kingsino Technology CO. LTDShenzhen 518107China. Shenzhen Institute of Nutrition and Health Huazhong Agricultural UniversityWuhan 430070China. Agricultural Genomics Institute at Shenzhen Chinese Academy of Agricultural SciencesShenzhen 518116China. College of Animal Science and National Engineering Research Center for Breeding Swine Industry South China Agricultural UniversityGuangzhou 510642China. Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642China.

Background:Intrauterine growth restriction(IUGR)is a major inducer of higher morbidity and mortality in the pig industry and catch-up growth(CUG)before weanling could significantly restore this negative influence.But there was limited knowledge about the underlying mechanism of CUG occurrence.Methods:Eighty litters of newborn piglets were divided into normal birth weight(NBW)and IUGR groups according to birth weight.At 26 d,those piglets with IUGR but over average body weight of eighty litters of weaned piglets were considered as CUG,and the piglets with IUGR still below average body weight were considered as NCUG.This study was conducted to systemically compare the intestinal difference among NBW,CUG and NCUG weaned piglets considering the crucial role of the intestine for piglet growth.Results:The results indicated that the m RNA expression of nutrients(amino acids,glucose,and fatty acids)transporters,and mitochondrial electron transport chain(ETC)I were upregulated in CUG piglets'gut with improved morphology compared with those NCUG,as well as the ratio of P-AMPK/AMPK protein expression which is the indicator of energy metabolism.Meanwhile,CUG piglet's gut showed higher antioxidative capacity with increased SOD and GSHPx activity,decreased MDA levels,as well as higher m RNA expressions of Nrf2,Keap1,SOD,and GSH-Px.Furthermore,inflammatory parameters including TNF-α,IL-1β,IL-6,and IL-12 factors,and the activation of MAPK and NF-κB signaling pathways were significantly elevated in the NCUG intestine,while the protein expression of ZO-1,Occludin and Claudin-1 was reduced.The alpha diversity of fecal microbiota was higher in CUG piglets in contrast with NCUG piglets,and the increased beneficial bacteria and decreased pathogenic bacteria was also observed in CUG piglets.Conclusions:CUG piglet's intestine showed comprehensive restoration including higher nutrients transport,energy metabolism,antioxidant capacity,and intestinal physical barrier,while lower oxidative stress,inflammatory response,and pathogenic microbi

关键词： Antioxidative capacity Catch-up growth Gut Intrauterine growth retardation Piglets

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亮氨酸对猪乳腺上皮细胞营养转运的影响及调控机制

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动物营养学报 2022年第3期34卷 1895-1907页

作者：朱鹏巍吴采池崔畅郑小雨马梓玮王俊张世海管武太陈芳华南农业大学动物科学学院国家生猪种业工程技术研究中心广州510642

本试验采用猪乳腺上皮细胞作为体外模型探讨亮氨酸对乳成分合成的影响及其分子机制。分别用0(对照组)、1、5和10 mmol/L的亮氨酸处理猪乳腺上皮细胞24和48 h后,检测细胞活力,分析乳蛋白、氨基酸转运载体、葡萄糖转运载体及脂肪酸转运载... 详细信息

本试验采用猪乳腺上皮细胞作为体外模型探讨亮氨酸对乳成分合成的影响及其分子机制。分别用0(对照组)、1、5和10 mmol/L的亮氨酸处理猪乳腺上皮细胞24和48 h后,检测细胞活力,分析乳蛋白、氨基酸转运载体、葡萄糖转运载体及脂肪酸转运载体的基因表达情况,并进一步检测哺乳动物雷帕霉素靶蛋白(mTOR)信号通路中关键蛋白mTOR和核糖体蛋白S6激酶1(S6K1)的表达和磷酸化情况以期探索体外亮氨酸调控乳成分合成的机制。结果显示:不同浓度亮氨酸处理48 h后细胞活力均较对照组显著提高(P0.05)。与对照组相比,1 mmol/L亮氨酸处理显著提高了S6K1的磷酸化水平(P0.05)。综上可知,在培养基中添加亮氨酸可提高猪乳腺上皮细胞活力,促进乳蛋白、氨基酸转运载体和脂肪酸转运载体基因的表达。

关键词：亮氨酸乳腺上皮细胞营养物质转运 mTOR通路

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快速热处理工艺下金属杂质对铸造多晶硅少子寿命的影响

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太阳能学报 2009年第5期30卷 611-614页

作者：陈玉武郝秋艳刘彩池赵建国吴丹王勇河北工业大学信息功能材料研究所天津300130

利用离子注入技术在铸造多晶硅中分别引入铜、铁、镍杂质玷污,用微波光电导衰减(μ-PCD)仪测试技术研究了铜、铁、镍杂质对硅片少子寿命的影响。研究发现:原生铸造多晶硅片和离子注入铜、铁、镍杂质的多晶硅片经1000℃常规热处理2h后少... 详细信息

利用离子注入技术在铸造多晶硅中分别引入铜、铁、镍杂质玷污,用微波光电导衰减(μ-PCD)仪测试技术研究了铜、铁、镍杂质对硅片少子寿命的影响。研究发现:原生铸造多晶硅片和离子注入铜、铁、镍杂质的多晶硅片经1000℃常规热处理2h后少子寿命值都会降低,下降幅度基本一致。原生硅片在低中温条件下,少子寿命值呈现先下降后上升的趋势;当硅片经高温RTP后,其少子寿命值得到明显改善。低中温RTP条件下,经铜、镍杂质玷污的硅片随着退火温度的升高,少子寿命变化不大。高温RTP条件下,经铜、铁、镍杂质玷污后硅片的少子寿命迅速下降。实验结果表明高温RTP能够提高杂质含量较低硅片的少子寿命,而对杂质含量较高硅片的少子寿命有负面影响。

关键词：铸造多晶硅 RTP 少子寿命杂质缺陷

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