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The multi-omics basis of potato heterosis

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Journal of Integrative Plant Biology 2022年第3期64卷 671-687页

作者： Dawei Li Xiaoyue lu Yanhui Zhu Jun Pan Shaoqun Zhou Xinyan Zhang Guangtao Zhu yi Shang Sanwen Huang Chunzhi Zhang Guangdong Laboratory of Lingnan Modern Agriculture Genome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Synthetic Biology CenterAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518172China Yunnan Key Laboratory of Potato Biology The AGISCAAS-YNNU Joint Academy of Potato SciencesYunnan Normal UniversityKunming 650500China

Heterosis is a fundamental biological phenomenon characterized by the superior performance of hybrids over their *** tremendous progress has been reported in seed crops,the molecular mechanisms underlying heterosis in clonally propagated crops are largely ***(Solanum tuberosum L.)is the most important tuber crop and an ongoing revolution is transforming potato from a clonally propagated tetraploid crop into a seed-propagated diploidhybrid *** our previous study,we developed the first generation of highly homozygous inbred lines of potato and hybrids with strong ***,we integrated transcriptome,metabolome,and DNA methylation data to explore the genetic and molecular basis of potato heterosis at three developmental *** found that the initial establishment of heterosis in diploid potato was mainly due to dominant *** color,male fertility,and starch and sucrose metabolism showed obvious gene dominant complementation in hybrids,and hybrids devoted more energy to primary metabolism for rapid *** addition,we identified~2700 allele-specific expression genes at each stage,which likely function in potato heterosis and might be regulated by CHH allele-specific methylation *** multi-omics analysis provides insight into heterosis in potato and facilitates the exploitation of heterosis in potato breeding.

关键词： allele-specific expression dominance DNA methylation heterosis metabolome multi-omics potato transcriptome

A New Convenient Synthesis of Pyridine-N-oxides

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Chinese Chemical Letters 1996年第10期7卷 907-908页

作者： Tao lu Xin Zhong SHI yi Ming WU(Dept.of Organic Chemistry, China Pharmaceutical University,Nanjing, Jiangsu 210009) CHINA PHARMACEUT UNIV DEPT ORGAN CHEMNANJING 210009JIANGSUPEOPLES R CHINA

This paper describes the direct oxidation of pyridines to pyridine-N-oxides by means of 30% H2O2 in aq media in the presence of Na2WO4.2H(2)O as catalyst instead of with per-acids or 30% H2O2 in acetic acid. Good yiel... 详细信息

关键词： A New Convenient Synthesis of Pyridine-N-oxides

A valine residue deletion in ZmSig2A, a sigma factor, accounts for a revertible leaf-color mutation in maize

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The Crop Journal 2021年第6期9卷 1330-1343页

作者： Chuan Li Jingwen Wang Zhaoyong Hu Yuanyan Xia Qiang Huang Tao Yu Hongyang yi Yanli lu Jing Wang Moju Cao Maize Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Chengdu 611130SichuanChina

A nuclear-encoded sigma(σ) factor is essential for the transcriptional regulation of plant chloroplastencoded genes. Five putative maize r factors have been identified by database searches, but their functions are unknown. We report a maize leaf color mutant etiolated/albino leaf 1(eal1) that was derived from space mutation breeding. The eal1 mutant displays etiolated or albino leaves that then gradually turn to normal green at the seedling stage. The changes in eal1 leaf color are associated with changes in photosynthetic pigment content and chloroplast development. Map-based cloning revealed that a single amino-acid deletion changing Val_(480)-Val_(481)-Val_(482) to Val_(480)-Val_(481), in the C-terminal domain σ_(4) of the putative σ factor ZmSig2A, is responsible for the eal1 mutation. In comparison with the expression level of the wild-type(WT) allele ZmSig2A^(+) in WT plants, much higher expression of the mutant allele ZmSig2A^(⊿V) in eal1 plants was detected before the eal1 plants turned to normal green. ZmSig2A shows the highest similarity to rice OsSig2A and Arabidopsis SIG2. Ectopic expression of ZmSig2A^(+) or ZmSig2A^(⊿V) driven by the cauliflower mosaic virus 35 S promoter rescued the pale green leaf of the sig2 mutant, but ectopic expression of ZmSig2A^(⊿V) driven by the SIG2 promoter did not. We propose that the Val deletion generated a new weak allele of ZmSig2A that cannot completely abolish the ZmSig2A function. Some genes involved in chloroplast development and photosynthesis-associated nuclear genes showed significant expression differences between eal1 and WT plants. We conclude that ZmSig2A encoding a r factor is essential for maize chloroplast development. The eal1 mutant with a weak allele of ZmSig2A represents a valuable genetic resource for investigating the regulation of ZmSig2A-mediated chloroplast development in *** eal1 mutation may be useful as a marker for early identification and elimination of false hybrids or transgene transm

关键词： Leaf color mutant Map-based cloning Sigma factor Chloroplast development Maize

新时期云南彝族作家文学艺术语言环境机制分析

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大理大学学报 2024年第3期9卷 49-55页

作者：鲁湘珺大理大学国际教育学院云南大理671003

艺术语言是发话主体在疏离和超越日常生活的状态中形成的感悟、体验、判断和推理。在结构意象、选择语言的过程中,也就不可避免地对客观之象进行夸张的变形,特别是对语言的解构。发话主体在艺术语言的创造过程中不仅受到语言本身、心理... 详细信息

艺术语言是发话主体在疏离和超越日常生活的状态中形成的感悟、体验、判断和推理。在结构意象、选择语言的过程中,也就不可避免地对客观之象进行夸张的变形,特别是对语言的解构。发话主体在艺术语言的创造过程中不仅受到语言本身、心理思维的影响,还会受到环境的制约和影响,其中就包含了民族性、地域性和文化传承性的影响。

关键词：云南彝族作家文学艺术语言环境机制

Copper-Catalyzed Aerobic Oxidative Cleavage of Unstrained Carbon-Carbon Bonds of 1,1-Disubstituted Alkenes with Sulfonyl Hydrazides

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Chinese Journal of Chemistry 2021年第4期39卷 859-865页

作者： Dong yi Linying He Zhongyu Qi Zhijie Zhang Mengshun Li Ji lu Jun Wei Xi Du Qiang Fu Siping Wei Department of Medicinal Chemistry School of PharmacySouthwest Medical UniversityLuzhouSichuan 646000China People’s Hospital of Xinjin District ChengduSichuan 611430China School of Pharmacy Binzhou Medical UniversityYantaiShandong 264003China Central Nervous System Drug Key Laboratory of Sichuan Province LuzhouSichuan 646000Chino

Main observation and conclusion Alkoxy radical-mediated carbon-carbon bond cleavages have emerged as a powerful strategy to complement traditional ionic-type ***,carbon-carbon cleavage reaction triggered by alkoxy radical intermediate derived from the combination of alkyl radical and dioxygen,is scarce and ***,we report alkoxy radical,which was generated from alkyl radical and dioxygen,mediated selective cleavage of unstrained carbon-carbon bond for the oxysulfonylation of 1,1-disubstituted alkenes,providing facile access to a variety of valuableβ-keto *** experiments indicated alkoxy radical intermediate that underwent subsequent regioselectiveβ-scission might be involved in the reaction and preliminary computational studies were conducted to provide a detailed explanation on the regioselectivity of the C-C bond ***,the strategy was successfully applied for constructing uneasily obtained architecturally intriguing molecules.

关键词： Carbon-carbon cleavage Alkoxy radical Copper-catalyzed 1,1-Disubstituted alkenes Sulfonyl hydrazides

黄连阿胶汤加减联合自制安神睡眠贴治疗失眠患者效果及对睡眠质量指数的影响

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现代生物医学进展 2024年第17期24卷 3237-3240,3364页

作者：蔡巍巍杜莹莹刘珊珊刘轶菲夏伟南京医科大学附属江宁医院中医科江苏南京211100

目的:研究使用中药方剂黄连阿胶汤联合自制安神睡眠贴治疗失眠患者临床疗效及对其睡眠质量指数的影响。方法:选取2021年1月到2023年12月在我院接受治疗103失眠患者,随机数表法分为对照组(51例)和研究组(52例),对照组患者予以常规西药治... 详细信息

目的:研究使用中药方剂黄连阿胶汤联合自制安神睡眠贴治疗失眠患者临床疗效及对其睡眠质量指数的影响。方法:选取2021年1月到2023年12月在我院接受治疗103失眠患者,随机数表法分为对照组(51例)和研究组(52例),对照组患者予以常规西药治疗,研究组患者在常规西药治疗的基础上加用黄连阿胶汤联合自制安神睡眠贴进行治疗,比较两组患者临床治疗疗效、治疗前后睡眠质量指数(PSQI)和生活质量(QOL)总分,以及治疗后全睡眠时间和睡眠觉醒次数。结果:研究组治疗后临床疗效有效率高于对照组(P<0.05)。两组患者治疗后PSQI总分均显著下降和QOL总分均上升,并且研究组与对照组比较有相同趋势(P<0.05)。治疗后,研究组患者全睡眠时间显著高于对照组患者(P<0.05),而睡眠觉醒次数显著低于对照组患者(P<0.05)。结论:黄连阿胶汤联合自制安神睡眠贴对失眠患者具有很好的临床治疗疗效,并且有助于降低其睡眠指数和提高生活质量,值得进行临床推广。

关键词：黄连阿胶汤安神睡眠贴失眠睡眠质量指数生活质量

载脂蛋白C3与糖尿病及糖尿病肾脏疾病的相关性研究进展

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临床肾脏病杂志 2024年第4期24卷 324-328页

作者：李灵芝蔡晓仪万启军张秀丽深圳大学附属第一医院(深圳市第二人民医院)肾内科深圳518025 暨南大学附属顺德医院肾内科佛山528306

糖尿病是由多病因引起的以慢性高血糖为特征的代谢性疾病。在糖尿病患者中,血脂累积可能引起脂质过氧化和细胞膜脂质氧化,进而导致细胞损害和功能障碍。脂质累积还可能导致糖尿病患者肾脏损害,如肾小球硬化、肾小管损伤以及足细胞消失... 详细信息

糖尿病是由多病因引起的以慢性高血糖为特征的代谢性疾病。在糖尿病患者中,血脂累积可能引起脂质过氧化和细胞膜脂质氧化,进而导致细胞损害和功能障碍。脂质累积还可能导致糖尿病患者肾脏损害,如肾小球硬化、肾小管损伤以及足细胞消失。载脂蛋白C3(plasma apolipoprotein C3,APOC3)是一种水溶性低分子糖蛋白,是人类脂质代谢调节的重要因子,通过胰岛素抵抗、脂代谢紊乱、炎症及氧化应激等途径参与糖尿病及相关并发症的发生发展。本文就APOC3在糖尿病及糖尿病肾脏疾病中的作用及机制进行如下综述。

关键词：载脂蛋白脂代谢糖尿病糖尿病肾脏疾病胰岛素抵抗

An easy-to-use site-directed mutagenesis method with a designed restriction site for convenient and reliable mutant screening

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2009年第6期10卷 479-482页

作者： Bao-zhong ZHANG Xin ZHANG Xiao-ping AN Duo-liang RAN Yu-sen ZHOU Jun lu yi-gang TONG State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology Beijing 100071 China College of Veterinary Science Xinltang Agricultural University Urumqi 830052 China Tumor Biotherapy Wurd Beijing YouAn Hospital Capital Medical University Beijing 100069 China

Site-directed mutagenesis (SDM) has been a very important method to probe the function-structure relationship of proteins. In this study, we introduced an easy-to-use, polymerase chain reaction (PCR)-based SDM method for double-stranded plasmid DNA, with a designed restriction site to ensure simple and efficient mutant screening. The DNA sequence to be mutated was first translated into amino acid sequence and then the amino acid sequence was reversely translated into DNA sequence with degenerate codons, resulting in a large number of sequences with silent mutations, which contained various restriction endonu-clease (RE) sites. Certain mutated sequence with an appropriate RE site was selected as the target DNA sequence for designing a pair of mutation primers to amplify the full-length plasmid via inverse PCR. The amplified product was 5′-phosphorylated, cir-cularized, and transformed into an Escherichia coli host. The transformants were screened by digesting with the designed RE. This protocol uses only one pair of primers and only one PCR is conducted, without the need for hybridization with hazardous isotope for mutant screening or subcloning step.

关键词： Site-directed mutagenesis (SDM) Restriction endonuclease Mutant screening

MiR-650通过靶向结合磷酸烯醇丙酮酸羧激酶1促进口腔鳞状细胞癌细胞增殖和糖酵解

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解剖学杂志 2024年第3期47卷 217-224,248页

作者：郭谊韩炎周丽芝王俊华马跃鲁大鹏唐山市中医医院口腔科唐山063011 文安县医院口腔科廊坊063000 唐山市第八医院口腔科唐山063000 文安县医院医院心内科廊坊063000 首都医科大学附属北京口腔医院急诊综合治疗中心北京100050

目的:分析miR-650是否靶向磷酸烯醇丙酮酸羧激酶1(PCK1)促进口腔鳞状细胞癌(OSCC)细胞增殖和糖酵解。方法:生物信息学筛选出差异表达且与细胞增殖、糖酵解有关的基因。构建稳定过表达PCK1的CAL-27和Tca8113细胞系,分成Vector组和PCK1组... 详细信息

目的:分析miR-650是否靶向磷酸烯醇丙酮酸羧激酶1(PCK1)促进口腔鳞状细胞癌(OSCC)细胞增殖和糖酵解。方法:生物信息学筛选出差异表达且与细胞增殖、糖酵解有关的基因。构建稳定过表达PCK1的CAL-27和Tca8113细胞系,分成Vector组和PCK1组。构建稳定过表达miR-650的OSCC细胞,分成miR-NC组、miR-650组和miR-650+PCK1组。CCK-8和克隆形成检测各组细胞增殖能力。裸鼠成瘤实验检测瘤体质量、体积。免疫组织化学检测肿瘤组织中PCK1表达水平。免疫印迹测定各组细胞PCK1蛋白表达水平。葡萄糖和乳酸试剂盒检测各组细胞内葡萄糖和乳酸变化。数据库预测PCK1靶标,双荧光酶素报告实验进行验证。RT-qPCR测定转染后OSCC细胞中miR-650的表达。结果:PCK1在OSCC中表达下调,且与细胞增殖和糖酵解相关。与Vector组相比,PCK1组中PCK1蛋白水平升高;过表达PCK1抑制细胞增殖;过表达PCK1抑制裸鼠移植瘤体积和质量增长;过表达PCK1促进细胞葡萄糖产生和抑制其消耗,同时抑制乳酸产生。与癌旁组织相比,miR-650在OSCC中表达上调。数据库预测及双荧光酶素报告实验证实miR-650与PCK1的靶向关系。与miR-NC组相比,miR-650组中PCK1 mRNA和蛋白表达水平明显降低。miR-650通过调节PCK1表达,促进OSCC细胞增殖,调控细胞糖酵解。结论:miR-650可以通过靶向结合PCK1促进OSCC细胞增殖和糖酵解。

关键词：口腔鳞状细胞癌磷酸烯醇丙酮酸羧激酶1 增殖糖酵解