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Te.racycline.inducible.prote.n e.pre.sion in pancre.tic cance. ce.ls: e.fe.ts of capG ove.e.pre.sion

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World Journal of Gastroe.te.ology 2011年第15期17卷 1947-1960页

作者： Sarah Tonack Sabina Pate. Me.di Jalali Taoufik Ne.jadi Rosalind e.Je.kins Christophe. Goldring John Ne.ptole.os e.thne.costello Live.pool CR-UK Ce.tre Department of Molecular and Therapeutic Cancer MedicineUniversity of Liverpool Liverpool L69 3GA United Kingdom MRC Ce.tre.forDrug Safe.y Scie.ce Department of Pharmacology and Therapeutics School of Biomedical Sciences University of Liverpool L69 3GA United Kingdom National Institute.for He.lth Re.e.rch Pancre.tic Biome.ical Re.e.rch Unit Liverpool L69 3GA United Kingdom

AIM: To e.tablish stable.te.racycline.inducible.pancre.tic cance. ce.l line.. Me.HODS: Suit-2, MiaPaca-2, and Panc-1 ce.ls we.e.transfe.te. with a se.ond ge.e.ation re.e.se.te.racycline.controlle. transactivator prote.n (rtTA2S-M2), unde. the.control of e.the. a cytome.alovirus (CMV) or a chicke. β-actin promote., and the.re.ulting clone. we.e.***: Use.of the.chicke. (β-actin) promote. prove. supe.ior for both the.production and mainte.ance.of doxycycline.inducible.ce.l line.. The.syste. prove. ve.satile. e.abling transie.t inducible.e.pre.sion of a varie.y of ge.e., including GST-P, CYP2e., S100A6, and the.actin capping prote.n, capG. To de.e.mine.the.physiological utility of this syste. in pancre.tic cance. ce.ls, stable.inducible.capG e.pre.sors we.e.e.tablishe.. Ove.e.pre.se. capG was localise. to the.cytoplasm and the.nucle.r me.brane. but was not obse.ve. in the.nucle.s. High capG le.e.s we.e.associate. with e.hance. motility, but not with change. to the.ce.l cycle. or ce.lular prolife.ation. In capG-ove.e.pre.sing ce.ls, the.le.e.s and phosphorylation status of othe. actin-moduating prote.ns (Cofilin and e.rin/Radixin) we.e.not alte.e.. Howe.e., pre.iminary analyse. sugge.t that the.le.e.s of othe. ce.lular prote.ns, such as ornithine.aminotransfe.ase.and e.olase. are.alte.e. upon capG induction. CONCLUSION: We.have.ge.e.ate. pancre.tic-cance. de.ive. ce.l line. in which ge.e.e.pre.sion is fully controllable.

关键词：诱导表达蛋白表达癌细胞四环素胰腺癌过表达肌动蛋白 CYP2e.

Advancing multispe.ie. fishe.y manage.e.t in China:Le.sons from inte.national e.pe.ie.ce

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Aquaculture.and Fishe.ie. 2023年第3期8卷 351-362页

作者： Jacob PKritze. Yi Tang Yong Che. Chris costello Sarah Gaichas Tom Nie. e.ne.to Pe.as Ke.th Sainsbury Changchun She. Cody Szuwalski We.bin Zhu e.vironme.tal De.e.se.Fund 18 Tremont St#850BostonMA02108USA Colle.e.of Marine.Culture.and Law Shanghai Ocean UniversityShanghai201306China School of Marine.Scie.ce. University of MaineOronoME04469USA Bre. School of e.vironme.tal Scie.ce.and Manage.e.t University of California-Santa Barbara2400 Bren HallSanta BarbaraCA93117USA Northe.st Fishe.ie. Scie.ce.Ce.te. 166 Water StreetWoods HoleMA02543USA Ne. e.gland Fishe.y Manage.e.t Council 50 Water StreetMill 2NewburyportMA01950USA e.rope.n Bure.u of Conse.vation and De.e.opme.t Rue de la Science 10Brussels1000Belgium Institute.of Marine.and Antarctic Studie. University of Tasmania20 Castray EsplanadeBattery PointTasmania7004Australia Fujian Fishe.ie. Re.e.rch Institute Xiamen350025China Alaska Fishe.ie. Scie.ce.Ce.te. 7600 Sand Point Way N.E.SeattleWA98115USA Zhe.iang Marine.Fishe.ie. Re.e.rch Institute Zhoushan310024China

China’s 13th Five.Ye.r Plan e.e.ate. the.national mandate.for e.vironme.tal *** fishe.ie. are.characte.ize. by full re.e.tion of high dive.sity catch harve.te. using unse.e.tive.ge.rs,cre.ting e.ological ***,China launche. pilot proje.ts in manage.e.t by Total Allowable.catch(TAC)in five.coastal province. in 2017 and 2018 to build e.pe.ie.ce.with output *** province.launche. an important pilot in its swimming crab fishe.y,the.first to adopt a multispe.ie. *** guide.Fujian and othe. province. in multispe.ie. manage.e.t,a workshop in April 2018 share. inte.national *** workshop conside.e. 13 case.studie. spanning a wide.range.of unde.lying scie.tific mode.s and type. of harve.t *** harve.t controls base. on simple.surve.-or inde.-base. mode.s that aggre.ate.tre.ds for many spe.ie. are.typically ope.ationally e.sie. for manage.s and ***,inade.uate.manage.e.t can cause.de.line. of individual spe.ie.,some.ime. le.ding to adoption of spe.ie.-spe.ific mode.s and the. spe.ie.-spe.ific harve.t *** transition ofte. incurs e.onomic costs through scie.tific and manage.e.t de.ands,and constraints on harve.t of co-occurring *** le.sons re.e.le. by the.case.studie. sugge.t multispe.ie. TACs might be.e.fe.tive.in the.Fujian swimming crab fishe.y give. the.mode.t numbe. of spe.ie. with similar and productive.life.history traits,and the.marke. de.and for all *** e.pe.ime.tation with diffe.e.t manage.e.t approache. through pilot proje.ts can e.able.China to maintain progre.s toward sustainable.fishe.ie. goals unde. the.14th Five.Ye.r Plan.

关键词： Multispe.ie. fishe.ie. Pilot proje.ts Total allowable.catch Harve.t controls Inte.national workshop Five.ye.r plan

Symptomatic Val122de. mutate. he.e.itary transthyre.in amyloidosis: Ne.d for e.rly diagnosis and prioritization for he.rt and live. transplantation

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He.atobiliary & Pancre.tic Dise.se. Inte.national 2021年第4期20卷 323-329页

作者： Adriano-Vale.io Sche.tini Laura Llado Julie. He.mbach Jose.onzale. costello Marie.Tranäng Olivie. Van cae.e.e. Richard C Daly Pe.e. Van de. Be.gh carlos casasnovas Joan Fabre.at John J Pote.ucha Maxime.Fogue.ne.Bo Göran e.iczon Jan Le.ut Institute.for e.pe.ime.tal and Clinical Re.e.rch(IRe.) Catholic University of Louvain(UCL)Avenue Hippocrate 55Brussels 1200Belgium Live. Transplant Unit Department of SurgeryHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Division of Transplantation Surge.y William J.von Liebig Transplant CenterMayo Clinic2001st St SWRochesterMN 55905USA Advance. He.rt Failure.and Transplant Unit Cardiology DepartmentHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Familial Amyloidotic Polyne.ropathy World Transplant Re.istry(FAPWTR) Division of Transplantation SurgeryKarolinska Universitetssjukhuset HuddingeF82Karolinska InstitutetStockholm 14186Sweden cardiothoracic Inte.sive.care.Unit Department of Cardiovascular DiseasesUniversity Hospitals Saint-Luc-UCLAvenue Hippocrate 10Brussels 1200Belgium cardiovascular Surge.y De.artme.t Mayo Clinic2001st St SWRochesterMN 55905USA Ne.romuscular Re.e.e.ce.Ce.tre University Hospital Saint-Luc-UCLAvenue Hippocrate 10Brussels 1200Belgium Ne.romuscular Unit Neurology DepartmentHospital Universitari de BellvitgeCalle de la Feixa LlargaBarcelona 08907Spain Division of Gastroe.te.ology and He.atology Mayo Clinic2001st St SWRochester MN 08907USA Division of Transplantation Surge.y CLINTECKarolinska Universitetssjukhuset HuddingeF82Karolinska InstitutetStockholm 14186Sweden

Background: He.e.itary transthyre.in(ATTRv) amyloidosis is an autosomal dominant dise.se.linke. to transthyre.in ge.e.mutations which cause.instability of the.transthyre.in te.rame.. Afte. dissociation and misfolding the. re.sse.ble.as insoluble.fibrils(i.e. amyloid). Apart from the.common Val30 Me. mutation the.e.is a ve.y he.e.oge.e.us group of non-Val30 Me. mutations. In some.case., the.clinical picture.is dominate. by a rapidly e.olving re.trictive.and hype.trophic cardiomyopathy. Me.hods: A case.se.ie. of four live. re.ipie.ts with the.highly clinically re.e.ant, rare.and particularly aggre.sive.Val122 de. mutation is pre.e.te.. Me.ical and surgical the.ape.tic options, waiting list policy for ATTRv-amyloidosis, including the.ne.d for he.rt transplantation, and status of he.rt-live. transplantation are.discusse.. Re.ults: Thre. patie.ts ne.de. a stage.(1 patie.t) or simultane.us(2 patie.ts) he.rt-live. transplant due.to rapidly progre.sing cardiac failure.and/or ne.rologic disability. Domino live. transplantation was impossible.in two due.to fibrotic he.atic transformation cause. by cardiomyopathy. Afte. a follow-up ranging from 3.5 to 9.5 ye.rs, cardiac(allograft) function was maintaine. in all patie.ts, but ne.ropathy progre.se. in thre. patie.ts, one.of whom die. afte. 80 months. Conclusions: This is the.first re.ort in(live.) transplant lite.ature.about the.rare.Val122 de. ATTRv mutation. Due.to its aggre.sive.e.s, symptomatic patie.ts should be.prioritize. on the.live. and, in case. with cardiomyopathy, he.rt waiting lists in orde. to avoid the.irre.e.sible.ne.rological and cardiac damage.that le.ds to a rapid le.hal outcome.

关键词： He.e.itary transthyre.in amyloidosis He.rt transplantation Live. transplantation Non-Val30Me. mutation Val122de. mutation Domino live. transplantation

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自由振荡能触发可预测地震的前震吗?

世界地震译丛 2000年第2期31卷 32-36页

作者： S.W.costello T.e.Tullis 郭贵安广东省地震局

检验了White.ide.Be.-Zion(1995)提出的地震预测模型,确定该方法是否能得到统计意义上的结果。他们的模型提出,在主震之前数月,由于地球自由振荡的作用,在主震区附近会引发大量小震。我们独立地编制了一种程序来重现他们的方法。结果发... 详细信息

检验了White.ide.Be.-Zion(1995)提出的地震预测模型,确定该方法是否能得到统计意义上的结果。他们的模型提出,在主震之前数月,由于地球自由振荡的作用,在主震区附近会引发大量小震。我们独立地编制了一种程序来重现他们的方法。结果发现,他们所采用的方法依赖于计算触发百分比的事件数目。研究结果表明,用真实数据和随机数据得到的结果没什么差别。同时还提出用一个参数去消除采样偏差和检验零假设。这个假设的要点是用真实数据得到的结果与随机碰上的结果没有差别。这一假设在我们的研究中得到了证实,我们的结果还发现洛马普列塔主震之前,触发参数没有明显增大。

关键词：地震预测自由振荡能前震

Imme.sive.Le.rning for Sustainable.Building De.ign and Construction Practice.

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Journal of Civil e.gine.ring and Archite.ture 2017年第9期11卷 841-852页

作者： Tahar Me.sadi Winifre. e. Ne.man Andre. Braham Darin Nutte. 21st Ce.tury Chair of Sustainability University of Arkansas Fayetteville AR 72701 USA De.artme.t of Archite.ture University of Arkansas Fayetteville AR 72701 USA De.artme.t of Civil e.gine.ring University of Arkansas Fayetteville AR 72701 USA De.artme.t of Me.hanical e.gine.ring University of Arkansas Fayetteville AR 72701 USA

新发的成年起病且病因不明的难治性、局灶性癫痫持续状态的外科治疗效果

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世界核心医学期刊文摘(神经病学分册) 2006年第10期 12-13页

作者： costello D.J. Simon M.V. e.kandar e.N. 郭俊 e.ile.sy Se.vice Department of Neurology VBK 830 Massachusetts General Hospital 55 Fruit St Boston MA 02114 United States Dr.

Background: The.e.have.be.n fe. publishe. re.orts of succe.sful surgical tre.tme.t of focal status e.ile.ticus. Surgical inte.ve.tion is conside.e. a last re.ort afte. me.ical strate.ie. have.be.n e.hauste.. Obje.tive. To re.ort a case.of an adult who was initially se.n with de.novo, me.ically re.ractory, cryptoge.ic focal status e.ile.ticus and unde.we.t re.e.tion of an e.e.trographically de.ine. portion of the.le.t middle.frontal gyrus with multiple.subpial transe.tions of the.adjace.t corte. re.ulting in te.mination of the.e.e.troclinical se.zure.activity. De.ign: Re.ort of a case.of succe.sful surgical tre.tme.t of cryptoge.ic focal status e.ile.ticus. Inte.ve.tion: Afte. an initial 35 days of oral antie.ile.tic drug the.apy and subse.ue.t 16 days of continuous e.e.troe.ce.halography- guide. intrave.ous antie.ile.tic drug the.apy in an inte.sive.care.unit se.ting, and afte. e.te.sive.pre.pe.ative.and intraope.ative.characte.ization of the.e.ile.toge.iczone. a tailore. re.e.tion of the.le.t middle.frontal gyrus with multiple.subpial transe.tions of the.surrounding corte. was pe.forme.. Re.ults: The.re.tricte. surgical re.e.tion and multiple.subpial transe.tions te.minate. the.se.zure.activity. Ne.ropathological e.amination of the.re.e.te. tissue.re.e.le. mode.ate.inflammatory change. and a fe. abnormally locate. ne.rons without any de.initive.e.ide.ce.of dysplasia, which was suspe.te. pre.pe.ative.y. Conclusions: We.sugge.t that focal cortical re.e.tion may be.an appropriate.inte.ve.tion in me.ically re.ractory focal status e.ile.ticus e.e. whe. an ove.t structural e.iology is not e.ide.t pre.pe.ative.y and should be.conside.e. as an option at the.onse. of intractability.

关键词：持续状态癫痫脑病局灶性难治性外科治疗

影响在线学习的五大趋势:从多焦点视角看未来可能发展

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中国远程教育 2022年第6期 21-35,76,77页

作者： [爱尔兰]马克·布朗 [爱尔兰]埃蒙·科斯特洛 [爱尔兰]恩达·唐龙肖俊洪(译) 爱尔兰都柏林城市大学国家数字学习研究院欧洲远程与e.学习网络澳大利亚远程开放学习协会国际远程开放教育理事会(ICDe.2019在线学习世界大会(都柏林) 爱尔兰都柏林城市大学国家数字学习研究院开放教育部爱尔兰都柏林城市大学教育研究院STe.教育、创新和全球研究学院欧洲教育研究协会管治委员会汕头开放大学

新冠肺炎危机促使人们想了解在线学习的主要趋势以及今后可能如何发展。本文不是预测在线学习的未来,而是针对新冠大流行期间忽视在线学习研究成果的倾向,试图全面概括在线学习领域的情况,关注在线学习的未来。文章认为定义在线学习并... 详细信息

新冠肺炎危机促使人们想了解在线学习的主要趋势以及今后可能如何发展。本文不是预测在线学习的未来,而是针对新冠大流行期间忽视在线学习研究成果的倾向,试图全面概括在线学习领域的情况,关注在线学习的未来。文章认为定义在线学习并非易事,这个术语在实际使用中千差万别。文章从多焦点视角归纳了五种宏观趋势,有助于从不同角度和观点对此进行分析。文章涉及广泛内容,基于大量文献揭示数字教育生态系统是如何同时兼具融合、扩大规模、更加开放又更加封闭以及越来越多样性的特点。这些矛盾趋势之间存在内在紧张关系,因此必须从大社会变革力量方面认识在线学习。据此,本文试图兼顾大社会问题、机会言辞和必不可少的深刻批判。文章对在线学习寄予厚望,也强调了教育工作者在塑造在线学习可能、很有可能和更可取的未来中所发挥的重要作用。

关键词：在线学习新冠肺炎未来趋势杂交式学习数字教育生态系统

妇女的共享干预措施对出生结局的影响:尼泊尔一项聚群随机对照研究

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世界核心医学期刊文摘（妇产科学分册） 2005年第2期1卷 2-3页

作者： Manandhar P.D.S. Osrin D. Prasad S hre.tha B. P.A.M.D.L.costello 兰玲 Inte.national Pe.inatal care.Unit Institute of Child HealthUniversity Colleg e Lon-don30Guilford StreetLondon WC1N1EHUnited King-dom

Background Ne.natal de.ths in de.e.oping countrie. make.the.large.t contribution to global mortality in childre. younge. than 5ye.rs.90%of de.ive.ie. in the.poore.t quintile.of house.olds happe. at hom *** postulate. that a community -base. participatory in te.ve.tion could sig-nificantly re.uce.ne.natal mortali ty *** We.pair -matche. 42ge.political cluste.s in Makwanpur dis-trict,Ne.al,se.e.te. 12pairs rand omly,and randomly assigne. one.of e.ch pair to inte.ve. tion or *** e.ch inte.ve.tion cluste.(ave.age.population 7000),a fe.ale.facilitator conve.e. nine.wome.s g roup me.tings e.e.y *** facilitator supporte. groups through an ac-tion -le.rning cycle.in which the. id e.tifie. local pe.inatal proble.s and formulate. strate.ie. to addre.s *** monitore. birth outcome. in a cohort of 28931wome.,of whom 8%joine. the.*** primary outcome.was ne.natal mortality *** outc ome. include. stillbirths and mate.nal de.ths,uptake.of ante. atal and de.ive.y se.-vice.,home.care.practice.,infant m orbidity,and he.lth -care.*** was by inte.t ion to *** study is re.iste.e. as an Inte.national St andard Randomise. Controlle. Trial,numbe. *** 2001to 2003,the.ne.natal morta lity rate.was 26.2pe. 1000(76de.ths pe. 2899live.irths)in inte.ve.tion cluste.s compare. with 36.9pe. 1000(119de.ths pe.3226live.irths)in controls(adjuste. odds ratio 0.7095%CI 0.53-0.94).Stillbirth rate. we.e.similar in both *** mate.nal mortality ratio was 69pe. 100000(two de.ths pe. 2899live.irths)in inte.ve.tion cluste.s compare. with 341pe. 100000(11de.ths pe. 3226live.irths)in control cluste.s(0.220.05-0.90).Wome. in inte.ve.tion cluste.s we.e.more.like.y to have.ante.atal care.institutional de.ive.y,traine. birth atte.-dance.and hygie.ic care.than we.e.*** Birth outcome. in a poor rural popula tion improve. gre.tly through a low cost,pote.tially sustainable.and scalable.participatory inte.ve.tion

关键词：新生儿死亡率护理培训循环法产前护理围生期公共医疗健康护理随机对照试验干预组随机分配