检索结果-南通市图书馆

Journal of Innovative Optical Health Sciences 2013年第3期6卷 99-107页

作者： cuicui liu YUEQING GU Department of Biomedical Engineering School of Life Science and Technology China Pharmaceutical University Nanjing 210009 P.R.China

The diagnosis of bacterial infections remains a major challenge in *** imaging of bacterial infection in living animals is usually conducted with genetic reporters such as lightemitting encymes or fluorescent ***,there are many circumstances where genetic reporters are not applicable,and there is an urgent need for exogenous synthetic probes that can selectively target *** imaging of bacteria in vivo is much less developed than methods such as ndioimaging and *** near infrared(NIR)dyes with emision wavelengths in the region of 650-900 rm can propagate through two or more centimeters of tissue and may enable deeper tisue imaging if sensitive detection techniques are *** we constructed an antimicrobial peptide fragment UBI29-41-based near infrared fuorescent imaging *** probe is composed of UBI2941 conjugated to a near infraured dye ICG ***29-41 is a cationic antimicrobial peptide that targets the anionic surfaces of bacterial *** probe allows detection of Staphylococcus aureus infection(5×10^(7)cells)in a mouse local infection model using whole animal nearinfrared fuorescence ***,we demonstrate that the UBI29-41-based imaging probe can selectively accumulate within *** significantly higher accumulation in bacterial infection suggests that UBI29-41-based imaging probe may be a promising imaging agent to detect bacterial infections.

关键词： Antimicrobial peptide bacterial targeting near infrared probe infection model in vriwo imaging

Numerical Simulation of Flow and Heat Transfer from Slot Jets Impinging on a Cylindrical Convex Surface

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Thermal Science 2011年第5期20卷 460-466页

作者： Zeyi JIANG cuicui liu Xinxin ZHANG Qiang MA Yusheng SUN School of Mechanical Engineering University of Science and Technology Beijing Beijing 100083 China China Aviation Planning and Construction Development Co LTD Beijing 102206 China

Flow and heat transfer characteristics of slot jets impingement to a cylindrical convex surface are numerically *** turbulence models have been determined through comparison with the experimental *** structures are described and impingement heat transfer characteristics are *** effects of Re,H/B and D/B on single-slot jets impingement heat transfer are analyzed and heat transfer characteristics of multiple-slot jets are *** results show that:Gas flows along the convex surface and boundary layer separation occurs in both single and multiple-slot jets impingement.A maximum stagnation Nu appears at H/B=8 and the local Nu decreases with increasing H/B in the region far away from the *** Nu in the stagnation region decreases with increasing D/B but the Nu is nearly the same in the region far away from the *** gradient is an important factor on heat transfer *** of the Num for single-slot,double-slot and quadric-slot jets impinging on a convex surface are *** indicates the effects of Re and D/B on Num could become more important in less slot jets impingement.

关键词： slot jets impingement convex surface numerical simulation flow and heat transfer

Recognition System for Leaf Diseases of Ophiopogon japonicus Based on PCA-SVM

在线全文

学校读者我要写书评

暂无评论

Plant Diseases and Pests 2020年第2期11卷 9-13页

作者： Yang Tao liu cuicui Chengdu Agricultural College Chengdu 611130China Sichuan Industrial Institute of Antibiotics Chengdu UniversityChengdu 610052China

Taking leaf black spot,anthracnose and leaf blight of Ophiopogon japonicus as the research objects,lesions were separated by K-Means clustering segmentation ***(principal component analysis)was carried out on the 46-dimensional eigenvectors composed of color,shape and texture features,and then the multi-level classifier designed by SVM(support vector machine)was used to identify *** recognition rate of the developed leaf disease recognition system of *** achieved 93.3%.The results indicates that the system is of great significance to the prevention and control of *** diseases and the modernization of *** industry.

关键词： Ophiopogon japonicus PCA SVM Disease recognition

A Novel Method of Auxiliary Power Supply Used in Wide-Range High Voltage Input DC-DC Converter

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Energy and Power Engineering 2017年第4期9卷 703-712页

作者： Jie Zhang Liangjie Li cuicui liu Hubei Collaborative Innovation Center for High-Efficiency Utilization of Solar Energy Hubei University of Technology Wuhan China

In high voltage input DC-DC converter, auxiliary winding of isolation transformer is usually used to supply power for control circuit. Due to the wide-range of input voltage, the variable output voltage of auxiliary winding will cause a series of problems, such as variable drive pulse amplitude, increased driver switching devices loss and drive transformer core saturation. This paper analyzes the influence of variable output voltage of auxiliary winding in detail. A novel method is proposed to solve the problem of large variation range of auxiliary winding output voltage, which is adding a buck converter between the auxiliary winding and the control circuit. A dual switch forward convert has been designed with 300 V - 800 V input and 24 V/5 A output. The results show that this method is effective by comparing the different results of using buck converter or not.

关键词： Dual Switch Forward Converter Wide-Range Input Auxiliary Winding of Power Supply Buck Converter

Identification and characterization of human hematopoietic mesoderm

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China(Life Sciences) 2024年第2期67卷 320-331页

作者： Yuqi Wen Jingjing Zhao Runqing Zhang Fan liu Xiaoyuan Chen Dan Wu Mengge Wang cuicui liu Pei Su Panpan Meng Yiyue Zhang Xin Gao Lu Wang Hongtao Wang Jiaxi Zhou State Key Laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Tianjin Institutes of Health Science Tianjin 301600China CAMS Center for Stem Cell Medicine PUMC Department of Stem Cell and Regenerative MedicineTianjin 300020China Division of Cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhou 510006China

The embryonic mesoderm comprises heterogeneous cell subpopulations with distinct lineage *** is unclear whether a bias for the human hematopoietic lineage emerges at this early developmental *** this study,we integrated single-cell transcriptomic analyses of human mesoderm cells from embryonic stem cells and embryos,enabling us to identify and define the molecular features of human hematopoietic mesoderm(HM)cells biased towards hematopoietic *** discovered that BMP4 plays an essential role in HM specification and can serve as a marker for HM ***,BMP4 acts as a downstream target of HDAC1,which modulates the expression of BMP4 by deacetylating its *** of HDAC significantly enhances HM specification and promotes subsequent hematopoietic cell *** conclusion,our study identifies human HM cells and describes new mechanisms for human hematopoietic development.

关键词： hematopoietic mesoderm human pluripotent stem cells human embryos BMP4 HDAC1

Introduction of nanoporous materials to capillary column and its application in a transverse electric field capillary electrochromatography

在线全文

学校读者我要写书评

暂无评论

Introduction of nanoporous materials to capillary column and...

Substitution Effect upon π···P and π···Br Noncovalent Interaction

第二十届全国色谱学术报告会及仪器展览会

作者： Mingshi Jin cuicui liu Donghao Li Key Laboratory of Natural Resource of the Changbai Mountain and Functional Molecular Ministry of Education and Department of Chemistry College of Science Yanbian University

＜正＞nanoporous materials,transverse electric field capillary electrochromatoraphy,on line separation Recently,separation of microscale materias（such as cells,biomacromaterials,particles and colloids）have been much...

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Substitution Effect upon π···P and π···Br Noncovalen...

中国化学会第十二届全国量子化学会议

作者： cuicui liu Shijun Zheng Xueying Zhang Institute of Computational Quantum Chemistry College of Chemistry and Material SciencesHebei Normal University

The importance of noncovalent interactions cannot be *** the noncovalent interactions,there is new interesting example that is comparable with the hydrogen bond and halogen bond;the case of pnicogen *** this work,the substitution effect on theπpnicogen and halogen bonds in C2H2···PH2X and C2H2···Br X（X=H,CH3,OH,Br,Cl,F,CN,and NO2）complexes were investigated using a high level ab initio *** quantum theory of atoms inmolecules（QTAIM）and the method of natural bond orbital（NBO）analysis were employed to examine the nature ofπ···P andπ···Br *** was found that two types of interaction are hallmarks of"closed-shell"and noncovalent *** is not only the electron-withdrawing substituents（CN,Br,Cl,NO2,and F）increase interaction forces but also the electron-donor substituents（CH3,OH）strengthen the *** interaction forces,forπ···P,climbs in the sequence X=H＜CH3＜OH＜CN＜Br＜Cl＜NO2＜*** pattern is variable to that ofπ···Br systems,particularly for the NO2 ***π···P interactions are weaker than,and in some cases comparable to the analogous halogen bonds except for the NO2 group.

关键词：

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

WS-CDL测试路径的生成与排序

东南大学学报（自然科学版） 2012年第3期42卷 428-434页

作者：刘翠翠邱栋李必信东南大学计算机科学与工程学院南京211189

为了找出WS-CDL组合流程中存在的错误,减少软件维护产生的开销,提出一种基于控制流图生成测试路径的方法,用于对WS-CDL进行测试.首先解析WS-CDL文档,根据元素特点生成各类节点,并根据流程结构确定节点的源节点和目标节点;然后通过在源... 详细信息

为了找出WS-CDL组合流程中存在的错误,减少软件维护产生的开销,提出一种基于控制流图生成测试路径的方法,用于对WS-CDL进行测试.首先解析WS-CDL文档,根据元素特点生成各类节点,并根据流程结构确定节点的源节点和目标节点;然后通过在源节点和目标节点间添加有向边构造成控制流图;最后通过遍历控制流图生成所有可能的测试路径.测试路径中覆盖的WS-CDL元素越多,则发现的错误数也可能越多.为提高发现错误的效率,提出了2种基于路径中元素数量的排序算法,对路径的执行顺序进行排序.实验结果表明,将测试路径按路径中未被覆盖的元素总数降序排列,可以更快地发现错误.

关键词： WS—CDL 控制流图测试路径排序

Study on Isolation and Degradation Characteristics of an Aniline-degrading Strain

在线全文

学校读者我要写书评

暂无评论

Meteorological and Environmental Research 2013年第4期4卷 46-48页

作者： Shimin Zhang Di Zhao Shuxia Xu cuicui liu Kun Wu College of Life Sciences Henan Agricultural University Key Laboratory of Enzyme Engineering of Agricultural Microbiology Ministry of Agriculture

[Objective] The research aimed to screen out an efficient aniline-degrading strain and study its degradation characteristics. [Method] By domesticated enrichment culture, an efficient aniline-degrading strain named as DA-K was isolated from activated sludge sample collected from a chemical plant in Henan. DA-K could use aniline as the sole carbon and nitrogen sources. The strain was carried out physiological and biochemi- cal identification, and its biological degradation characteristics were studied. [ Result] DA-K strain was G - and red-shaped, and its colony color was off-white. It was initially determined as Acinetobacter sp. By measuring, the optimum growth temperature and pH for DA-K were respectively 30 ~C and 6.0. DA-K strain could grow well in inorganic salt medium with aniline of 2 500 mg/L. After shaking for 96 h under the conditions of pH 6.0, 30 ℃, 180 r/min and 1 000 mg/L of aniline, the degradation rate of the aniline by DA-K could reach nearly 80%. [ Conclusion] The DA-K strain had a higher aniline degradation efficiency and actual treatment capability of the aniline wastewater, which laid foundation for establishing gene engineerincl strain.

关键词： Aniline-degrading strain Isolation and screening Degradation characteristics China

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

自噬相关蛋白在皮质发育畸形病变中的表达

中华病理学杂志 2015年第5期44卷 305-309页

作者：陈诗赟首都医科大学宣武医院病理科北京100053

目的检测自噬相关蛋白Beclin-1、LC3及p62在难治性癫痫相关皮质发育畸形病变中的表达情况，初步探讨皮质发育畸形出现细胞水平异常的可能机制。方法选用18例手术切除的致痫灶标本，包括结节硬化综合征(TSC)、局灶性皮质发育不良(FCD)11... 详细信息

目的检测自噬相关蛋白Beclin-1、LC3及p62在难治性癫痫相关皮质发育畸形病变中的表达情况，初步探讨皮质发育畸形出现细胞水平异常的可能机制。方法选用18例手术切除的致痫灶标本，包括结节硬化综合征(TSC)、局灶性皮质发育不良(FCD)11b型及FCDI型各6例。采用免疫组织化学染色(EnVison二步法)对3种蛋白分别在3组病变中细胞水平的表达情况进行定性分析，并计数Beclin-1、LC3染色阳性细胞数进行比较；应用Westernblot方法对LC3蛋白在3组病变中的表达进行定量比较分析。结果免疫组织化学染色结果显示3种蛋白均主要表达于FCDIIb型及TSC组中的形态异常神经元和气球样细胞／巨大细胞内。Beclin-1为胞质内颗粒状或弥漫阳性，另可见到轴索强阳性表达；LC3为胞质内弥漫阳性或核周阳性；p62为胞质内弥漫阳性，部分为核及核周阳性。此外，形态异常神经元中Beclin-1、LC3及p62阳性程度高于气球样细胞／巨大细胞。而作为对照的FCDI组中仅有个别或部分细胞呈弱阳性表达。Beclin-1和LC3染色中阳性细胞数均为TSC组>FCDIIb组>FCDI组，且TSC组、FCDIIb组与FCDI组的差异均具有统计学意义(P<0．05)。Westernblot结果显示LC3蛋白在病灶组织内的表达量为TSC组

关键词：皮质发育畸形自噬癫痫