检索结果-南通市图书馆

Effect of tubastatin A on NLRP3 inflammasome activation in macrophages under hypoxia/ reoxygenation conditions

World Journal of Emergency Medicine 2024年第4期15卷 289-296页

作者： Hao Li Chang Liu ying cui Panpan Chang Wei Chong Department of Emergency Medicine the First Hospital of China Medical UniversityShenyang 110001China Trauma Medicine Center Peking University People's HospitalBeijing 100871China Key Laboratory of Trauma and Neural Regeneration Peking UniversityBeijing 100871China National Center for Trauma Medicine of China Beijing 100871China

BACKGROUND:There are currently no effective drugs to mitigate the ischemia/reperfusion injury caused by fluid resuscitation after hemorrhagic shock(HS).The aim of this study was to explore the potential of the histone deacetylase 6(HDAC6)-specific inhibitor tubastatin A(TubA)to suppress nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)inflammasome activation in macrophages under hypoxia/reoxygenation(H/R)***:The viability of RAW264.7 cells subjected to H/R after treatment with different concentrations of TubA was assessed using a cell-counting kit-8(CCK8)***,2.5μmol/L TubA was used with RAW264.7 cells under H/R ***264.7 cells were divided into three groups,namely the control,H/R,and TubA *** levels of reactive oxygen species(ROS)in the cells were detected using fluorescence *** protein expression of HDAC6,heat shock protein 90(Hsp90),inducible nitric oxide synthase(iNOS),NLRP3,gasdermin-D(GSDMD),Caspase-1,GSDMD-N,and Caspase-1 p20 was detected by western *** levels of interleukin-1β(IL-1β)and IL-18 in the supernatants were detected using enzyme-linked immunosorbent assay(ELISA).RESULTS:HDAC6,Hsp90,and iNOS expression levels were significantly higher(P<0.01)in the H/R group than in the control group,but lower in the TubA group than in the H/R group(P<0.05).When comparing the H/R group to the control group,ROS levels were significantly higher(P<0.01),but significantly reduced in the TubA group(P<0.05).The H/R group had higher NLRP3,GSDMD,Caspase-1,GSDMD-N,and Caspase-1 p20 expression levels than the control group(P<0.05),however,the TubA group had significantly lower expression levels than the H/R group(P<0.05).IL-1βand IL-18 levels in the supernatants were significantly higher in the H/R group compared to the control group(P<0.01),but significantly lower in the TubA group compared to the H/R group(P<0.01).CONCLUSION:TubA inhibited the expression of HDAC6,Hsp90,and iNOS in macrophages

关键词： Hemorrhagic shock Hypoxia/reoxygenation Macrophage NLRP3 Tubastatin A

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Uncertainties of nuclear level density estimated using Bayesian neural networks

引用

Chinese Physics C 2024年第8期48卷 185-189页

作者： Xinyu Wang ying cui Yuan Tian Kai Zhao yingxun Zhang China Institute of Atomic Energy Beijing 102413China China Nuclear Data Center China Institute of Atomic EnergyBeijing 102413China Guangxi Key Laboratory of Nuclear Physics and Technology Guangxi Normal UniversityGuilin 541004China

Nuclear level density(NLD)is a critical parameter for understanding nuclear reactions and the structure of atomic nuclei;however,accurate estimation of NLD is challenging owing to limitations inherent in both experimental measurements and theoretical *** paper presents a sophisticated approach using Bayesian neural networks(BNNs)to analyze NLD across a wide range of *** uniquely incorporates the assessment of model *** application of BNNs demonstrates remarkable success in accurately predicting NLD values when compared to recent experimental data,confirming the effectiveness of our *** reliability and predictive power of the BNN approach not only validates its current application but also encourages its integration into future analyses of nuclear reaction cross sections.

关键词： Bayesian neural network nuclear reaction nuclear level density

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

RsaL is a self-regulatory switch that controls alternative biosynthesis of two AHL-type quorum sensing signals in Pseudomonas aeruginosa PA1201

引用

mLife 2024年第1期3卷 74-86页

作者： Ya-Wen He Zi-Jing Jin ying cui Kai Song Bo Chen Lian Zhou State Key Laboratory of Microbial Metabolism Joint International Research Laboratory of Metabolic and Developmental SciencesSJTU-NLBP Joint R&D Centre for Biopesticides and BiofertilizersSchool of Life Sciences and BiotechnologyShanghai Jiao Tong UniversityShanghaiChina Zhiyuan Innovative Research Centre Student Innovation CentreZhiyuan CollegeShanghai Jiao Tong UniversityShanghaiChina

Pseudomonas aeruginosa is a ubiquitous and metabolically versatile microorganism naturally found in soil and *** is also an opportunistic pathogen in plants,insects,animals,and *** response to increasing cell density,*** uses two acylhomoserine lactone(AHL)quorum-sensing(QS)signals(i.e.,N-3-oxo-dodecanoyl homoserine lactone[3-oxo-C12-HSL]and Nbutanoyl-homoserine lactone[C4-HsL]),which regulate the expression of hundreds of ***,how the biosynthesis of these two QS signals is coordinated remains *** studied the regulation of these two QS signals in the rhizosphere strain ***1201 sequentially produced 3-oxo-C12-HSL and C4-HSL at the early and late growth stages,*** highest 3-oxo-C12-HSL-dependent elastase activity was observed at the early stage,while the highest C4-HSL-dependent rhamnolipid production was observed at the late *** atypical regulator RsaL played a pivotal role in coordinating 3-oxo-C12-HSL and C4-HSL biosynthesis and QS-associated *** repressed las/transcription by binding the-10 and-35 boxes of the lasl *** contrast,RsaL activated rhll transcription by binding the region encoding the 5'-untranslated region of the rhll ***,RsaL repressed its own expression by binding a nucleotide motif located in the-35 box of the rsaL ***,RsaL acts as a molecular switch that coordinates the sequential biosynthesis of AHL QS signals and differential virulence in ***,C4-HSL activation by RsaL was independent of the Las and Pseudomonas quinolone signal(PQS)QS signaling ***,we propose a new model of the QS regulatory network in PA1201,in which RsaL represents a superior player acting at the top of the hierarchy.

关键词： N-3-oxo-dodecanoyl homoserine lactone N-butanoyl-homoserine lactone Pseudomonas aeruginosa quorum sensing RsaL

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Low-intensity pulsed ultrasound reduces alveolar bone resorption during orthodontic treatment via Lamin A/C-Yes-associated protein axis in stem cells

引用

World Journal of Stem Cells 2024年第3期16卷 267-286页

作者： Tong Wu Fu Zheng Hong-Yi Tang Hua-Zhi Li Xin-Yu cui Shuai Ding Duo Liu cui-ying Li Jiu-Hui Jiang Rui-Li Yang Department of Orthodontics Peking University School and Hospital of StomatologyNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory of Digital StomatologyBeijing 100081China Department of Central Laboratory Peking University School and Hospital of StomatologyNational Clinical Research Center for Oral Diseases&National Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory of Digital StomatologyBeijing 100081China

BACKGROUND The bone remodeling during orthodontic treatment for malocclusion often requires a long duration of around two to three years,which also may lead to some complications such as alveolar bone resorption or tooth root ***-intensity pulsed ultrasound(LIPUS),a noninvasive physical therapy,has been shown to promote bone fracture *** is also reported that LIPUS could reduce the duration of orthodontic treatment;however,how LIPUS regulates the bone metabolism during the orthodontic treatment process is still *** To investigate the effects of LIPUS on bone remodeling in an orthodontic tooth movement(OTM)model and explore the underlying *** A rat model of OTM was established,and alveolar bone remodeling and tooth movement rate were evaluated via micro-computed tomography and staining of tissue *** vitro,human bone marrow mesenchymal stem cells(hBMSCs)were isolated to detect their osteogenic differentiation potential under compression and LIPUS stimulation by quantitative reverse transcription-polymerase chain reaction,Western blot,alkaline phosphatase(ALP)staining,and Alizarin red *** expression of Yes-associated protein(YAP1),the actin cytoskeleton,and the Lamin A/C nucleoskeleton were detected with or without YAP1 small interfering RNA(siRNA)application via *** The force treatment inhibited the osteogenic differentiation potential of hBMSCs;moreover,the expression of osteogenesis markers,such as type 1 collagen(COL1),runt-related transcription factor 2,ALP,and osteocalcin(OCN),*** could rescue the osteogenic differentiation of hBMSCs with increased expression of osteogenic marker inhibited by ***,the expression of LaminA/C,F-actin,and YAP1 was downregulated after force treatment,which could be rescued by ***,the osteogenic differentiation of hBMSCs increased by LIPUS could be attenuated by YAP siRNA ***,LIPUS increased alveolar

关键词： Low-intensity pulsed ultrasound Bone resorption Osteogenesis Cytoskeleton-Lamin A/C-Yes-associated protein axis Bone marrow mesenchymal stem cells Orthodontic tooth movement

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pan-African metamorphism and magmatism in the Prydz Belt,East Antarctica:a geochronological perspective

引用

Advances in Polar Science 2024年第2期35卷 143-156页

作者： BAO Hong WANG Wei-(RZ) LIU Xinshu ZHAO Yue GONG Tingnan LIU Xiaochun cui ying TIAN Zuolin Institute of Geomechanics Chinese Academy of Geological SciencesBeijing 100081China Key Laboratory of Paleomagnetism and Tectonic Reconstruction Ministry of Natural ResourcesBeijing 100081China China University of Geosciences(Beijing) Beijing 100081China MOE Key Laboratory of Orogenic Belt and Crustal Evolution School of Earth and Space SciencesPeking UniversityBeijing 100871China Institute of Geology Chinese Academy of Geological SciencesBeijing 100037China

The Prydz Belt in East Antarctica underwent extensive reworking during the late Neoprotertozoic–early Paleozoic Pan-African orogeny,which is characterized by granulite facies,clockwise P–T paths,and high dT/dP *** study compiles the existing age and composition data of zircon and monazite from metamorphic rocks and links their key characteristics to the metamorphic evolution of the Prydz *** frequency of zircon U–Pb ages starts to increase noticeably from~555 Ma,peaking between 530 Ma and 520 Ma,followed by a dramatic decline after 520 *** Th/U values(>0.1)of zircon are observed from~545 Ma,displaying a noticeable increasing trend in Th/U values before a rapid decline from~520 *** frequency of monazite ages progressively increases from~540 Ma,reaching its peak at 515 Ma,and then rapidly decreases after 490 *** with the crystallization behaviors of zircon and monazite,this study suggests that the systematic changes in Th/U values of zircon after 545 Ma indicate a transition in the thermal regime of the Prydz Belt towards the cooling *** growth of zircon and monazite corresponds to the post-peak cooling process,while the crystallization peak of monazite lags behind that of zircon by~5–15 Ma,which indicates a relatively low cooling *** the granitic intrusions accompanied the entire metamorphic evolution,the majority of them are younger than 520 *** results suggest that the Pan-African event likely peaked at~555–545 Ma and gradually cooled to near-solidus conditions at~520–510 Ma,with a relatively slow average cooling process.

关键词： East Antarctica Prydz Belt Pan-African orogeny zircon monazite U–Pb dating

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

N-glycan biosignatures as a potential diagnostic biomarker for earlystage pancreatic cancer

引用

World Journal of Gastrointestinal Oncology 2024年第3期16卷 659-669页

作者： Yan-Rong Wen Xia-Wen Lin Yu-Wen Zhou Lei Xu Jun-Li Zhang cui-ying Chen Jian He Department of Nuclear Medicine Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008Jiangsu ProvinceChina Department of Research and Development Sysdiagno(Nanjing)Biotech Co.LtdNanjing 210008Jiangsu ProvinceChina

BACKGROUND Pancreatic ductal adenocarcinoma(PDAC)has a poor prognosis,with a 5-year survival rate of less than 10%,owing to its late-stage *** detection of pancreatic cancer(PC)can significantly increase survival *** To identify the serum biomarker signatures associated with early-stage PDAC by serum N-glycan *** An extensive patient cohort was used to determine a biomarker signature,in-cluding patients with PDAC that was well-defined at an early stage(stages I and II).The biomarker signature was derived from a case-control study using a case-cohort design consisting of 29 patients with stage I,22 with stage II,4 with stage III,16 with stage IV PDAC,and 88 *** used multiparametric analysis to identify early-stage PDAC N-glycan signatures and developed an N-glycan sig-nature-based diagnosis model called the“Glyco-model”.RESULTS The biomarker signature was created to discriminate samples derived from patients with PC from those of controls,with a receiver operating characteristic area under the curve of *** addition,the biomarker signature combined with cancer antigen 19-9 could discriminate patients with PDAC from controls,with a receiver operating characteristic area under the curve of ***-model demonstrated favorable diagnostic performance in all stages of *** diagnostic sensitivity for stage I PDAC was 89.66%.Core Tip:This study employed a patient cohort to investigate the N-glycan signature of early-stage pancreatic cancer(PC).Serum N-glycans analysis was conducted to identify the serum biomarker signature associated with early-stage pancreatic ductal adenocarcinoma(PDAC),resulting in the identification of nine early-stage PDAC N-glycan ***,utilizing these biosignatures,a diagnostic model named the“Glyco-model”was developed,demonstrating promising diagnostic performance across all stages of *** study revealed that the diagnostic sensitivity for stage I PDAC was determined to be 89.66%.Co

关键词： Glycomics N-glycans Biomarkers Pancreatic cancer Predictive modeling

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

古新世-始新世极热事件(PETM)浅海生态系统稳态转变:以塔里木盆地库孜贡苏剖面钙质超微浮游植物为例

引用

第四纪研究 2024年第5期44卷 1205-1214页

作者：梁瑞泽华春荣王亚苏苏虹姜仕军 cui ying 河海大学海洋学院江苏南京210098 阿荣旗第二中学内蒙古呼伦贝尔162750 暨南大学生命科学技术学院广东广州510632 Montclair State University USA Montclair NJ 07043

古新世-始新世极热事件(PETM)发生于56 Ma前,因其碳释放过程与当前人类CO_(2)持续排放驱动的全球变暖类似而备受世人关注。塔里木盆地保存完好、出露完整且连续的古近纪浅海地层为研究PETM期间东特提斯洋古环境变化提供了理想材料,但目... 详细信息

古新世-始新世极热事件(PETM)发生于56 Ma前,因其碳释放过程与当前人类CO_(2)持续排放驱动的全球变暖类似而备受世人关注。塔里木盆地保存完好、出露完整且连续的古近纪浅海地层为研究PETM期间东特提斯洋古环境变化提供了理想材料,但目前缺乏对PETM前后塔里木盆地浮游植物群落稳态转变的研究。本研究利用库孜贡苏剖面齐姆根组约48 m厚海相沉积物中保存的丰富钙质超微化石群落,研究PETM前后生态系统演变过程,揭示PETM事件前后发生了两次稳态转变。第一次稳态转变发生在沉积厚度约为19.61~24.56 m之间,与PETM事件开始时间恰好对应;第二次稳态转变发生在沉积厚度约为29.60~31.06 m之间,与PETM事件恢复期基本对应。PETM事件前Coccolithus pelagicus、Toweius pertusus和***等钙质超微化石占主导地位,当稳态开始发生改变时,***和***相对丰度开始下降,而“负偏属种”Discoaster araneus和Discoaster acutus开始出现。P和Zn/Al等营养指标升高,表明东特提斯洋地区在PETM期间初级生产力增加,高生产力指示种Neochiastozygus junctus在PETM期间显著增加也印证了这个观点。北大西洋中部滨海平原沉积记录表明,其生态系统在PETM时期同样经历了两次稳态变化,与库孜贡苏剖面两次转变时间基本对应,表明由PETM事件导致的浅海生态系统稳态转变可能是全球而非区域性变化。

关键词： PETM 钙质超微化石稳态转变塔里木盆地东特提斯洋

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

古新世-始新世极热事件(PETM)浅海生态系统稳态转变:以塔里木盆地库孜贡苏剖面钙质超微浮游生物为例

引用

第四纪研究 2024年

作者：梁瑞泽华春荣王亚苏苏虹姜仕军 cui ying 河海大学海洋学院阿荣旗第二中学暨南大学生命科学技术学院 Montclair State University

发生在56 Ma前的古新世-始新世极热事件（PETM）因其碳释放过程与当前人类CO2持续排放驱动的全球变暖类似而备受世人关注。塔里木盆地保存完好、出露完整且连续的古近纪浅海地层为研究PETM期间副特提斯洋古环境变化提供了理想场所,但目... 详细信息

发生在56 Ma前的古新世-始新世极热事件（PETM）因其碳释放过程与当前人类CO2持续排放驱动的全球变暖类似而备受世人关注。塔里木盆地保存完好、出露完整且连续的古近纪浅海地层为研究PETM期间副特提斯洋古环境变化提供了理想场所,但目前缺乏对PETM期间塔里木盆地浮游植物群落稳态转变的研究。本研究利用库-孜贡苏剖面齐姆根组约48m厚的丰富钙质超微化石群落对PETM期间生态系统稳态转变进行探究发现,在PETM事件前后发生过两次稳态转变。第一次稳态转变发生在沉积厚度约为19.61～24.56 m之间,与PETM事件开始时间恰好对应;第二次稳态转变发生在沉积厚度约为29.60～31.06 m之间,与PETM事件恢复期基本对应。PETM事件前主要由(Coccolithus pelagicus、Toweius pertusus和***等钙质超微化石占主导地位,当稳态开始发生改变时,***和***相对丰度开始下降,而“负偏种群”Discoaster araneus和Discoaster acutus开始出现。P和Zn/Al等营养元素含量增加表明了副特提斯洋地区在PETM期间海洋初级生产力增加,代表高生产力指标的钙质超微化石Neochiastozygus junctus在PETM期间显著增加也印证了这个观点。北大西洋中部滨海平原沉积记录表明,其生态系统在PETM时期同样经历了两次稳态变化,与库孜贡苏剖面两次转变时间基本对应,表明由PETM事件导致的浅海生态系统稳态转变可能是全球性行为而非区域性变化。

关键词： PETM 钙质超微化石稳态转变塔里木盆地

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

马恩政治哲学的贡献、价值及其当代意义

引用

华中科技大学学报（社会科学版） 2024年第3期38卷 12-21页

作者：江畅周莹萃湖北大学哲学学院

马克思和恩格斯共同创立的政治哲学(后文简称为“马恩政治哲学”)对人类政治哲学和政治文明发展作出了独特贡献,具有不可替代的独特价值。这些价值对于推进中国式现代化建设、人类文明新形态完善和对于人类命运共同体构建具有重大现实... 详细信息

马克思和恩格斯共同创立的政治哲学(后文简称为“马恩政治哲学”)对人类政治哲学和政治文明发展作出了独特贡献,具有不可替代的独特价值。这些价值对于推进中国式现代化建设、人类文明新形态完善和对于人类命运共同体构建具有重大现实意义。马恩政治哲学创建了以人类彻底解放和普遍幸福为终极追求的政治哲学体系,描绘了具有现实可行性的美好理想社会,找到了实现理想蓝图的强大社会力量,开辟了破坏旧世界、建设新世界的可靠路径。马恩政治哲学给政治哲学提供了唯物史观的理论基础,确立了全人类立场和价值取向,将每一个个人全面而自由的发展规定为政治的终极目的,提出了一系列具有普遍性的核心理念和基本原则,明确了政治哲学改变世界的根本使命和实践要求。马恩政治哲学研究和回答了一系列重大政治问题,其政治本性论为政治文明进步奠定了牢固的动力基础,政治目的论为人类普遍而永久获得幸福指明了正确前进道路,政治制度论为社会长治久安贡献了政治哲学智慧,政治活动理论为实行人民统治和治理提供了可行的实践方案,政治权力论为人民当家作主提供了充分理论论证。

关键词：马恩政治哲学唯物史观人类立场人的全面而自由的发展

来源：

维普期刊数据库博看期刊

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Metagenomics analysis identifies oral Streptococcus as potential biomarkers for nasopharyngeal carcinoma

引用

Journal of Genetics and Genomics 2024年第3期51卷 363-366页

作者： Yanmin Liu Chengtao Wang Lixiang Xie Shuling Du Li Ding ying cui Runzhi Chen Jingxiang Zhang Wan Wang Xi Liu Yan Wang Shiyan Chen Tian Tan Qiaochu Zhao Limei Yin Chunwei Li Yong Chen Tao Ding Department of Immunology and Microbiology Zhongshan School of MedicineSun Yat-sen UniversityGuangzhouGuangdong 510080China Key Laboratory of Tropical Disease Control(Sun Yat-sen University) Ministry of EducationGuangzhouGuangdong 510080China Department of Radiation Oncology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510080China Department of Infectious Diseases The Fifth Affiliated HospitalSun Yat-sen UniversityZhuhaiGuangdong 519000China Department of Endocrinology&Metabolism The Fifth Affiliated HospitalSun Yat-sen UniversityZhuhaiGuangdong 519000China Department of Otolaryngology The First Affiliated HospitalSun Yat-sen UniversityGuangzhouGuangdong 510080China

Nasopharyngeal carcinoma(NPC)is a malignancy originating from the nasopharyngeal epithelial *** some studies have identified potential biomarkers for early diagnosis of NPC based on metabolomics data(Chen et al.,2023;Zhou et al.,2023)and Epstein-Barr virus subtypes(Xu et al.,2019),their effectiveness and applicability need further investigation,particularly when consideringthe roles of oral microbiota.

关键词： al. nasopharyngeal diagnosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：