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A new approach to promote astaxanthin accumulation via Na_2WO_4 in Haematococcus pluvialis

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Journal of Oceanology and Limnology 2019年第1期37卷 176-185页

作者： LI Yanguo cui DANDan zhuo Pinli ZHANG Lin SUN Xue XU Nianjun Key Laboratory of Marine Biotechnology of Zhejiang Province School of Marine Sciences Ningbo University

The optimum concentration of Na_2 WO_4 was explored in relation to the cell density and astaxanthin content in Haematococcus pluvialis. Then, the cellular morphology, nitrate reductase(NR) activity, soluble sugar and protein contents, and chlorophyll ?uorescence were measured, and the transcriptional expression of carotenogenic genes was determined by quantitative real-time PCR. The results showed that 3.0 mmol/L of Na_2 WO_4 was the optimum concentration to induce astaxanthin accumulation, with a maximum content of 49.41±0.13 pg/cell reached on the tenth day. The NR activity decreased signi?cantly and continually after Na_2 WO_4 treatment. The soluble sugar content increased gradually during the experimental period and was eventually signi?cantly higher than that in the control. The soluble protein content increased rapidly,reached a maximum in day 0.5 and day 1 and then decreased. The ef fective photochemical effciency of PSII( F v'/F m') and light saturation( E k) ?rst decreased and then tended to stabilize, and NADP +-glyceraldehyde-3-phosphate dehydrogenase(GAPDH) gene expression was correlated with photosynthesis. The transcriptional expression of ipi, psy and bkt was signi?cantly increased compared with that in the control after application of Na_2 WO_4, and the relative expression of ipi reached the highest level on the ?fth day, with a 98.03±1.92-fold increase. Our results describe a new approach to promote the ef fective accumulation of astaxanthin in H. pluvialis by NR inhibitor Na_2 WO_4.

关键词： Haematococcus pluvialis astaxanthin Na2WO4 nitrate reductase quantitative real-time PCR

A LOW-REGULARITY FOURIER INTEGRATOR FOR THE DAVEY-STEWARTSON II SYSTEM WITH ALMOST MASS CONSERVATION

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Acta Mathematica Scientia 2024年第4期44卷 1536-1549页

作者： cui ning Chenxi HAO Yaohong WANG School of Financial Mathematics and Statistics Guangdong University of FinanceGuangzhou 510521China Center for Applied Mathematics Tianjin UniversityTianjin 300072China

In this work,we propose a low-regularity Fourier integrator with almost mass conservation to solve the Davey-StewartsonⅡsystem(hyperbolic-elliptic case).Arbitrary order mass convergence could be achieved by the suitable addition of correction terms,while keeping the first order accuracy in H~γ×H^(γ+1)for initial data in H^(γ+1)×H^(γ+1)withγ>*** main theorem is that,up to some fixed time T,there exist constantsτ_(0)and C depending only on T and‖u‖_(L^(∞)((0,T);H^(γ+1)))such that,for any 0<τ≤τ_(0),we have that‖u(t_(n),·)-u^(n)‖H_γ≤C_(τ),‖v(t_(n),·)-v^(n)‖_(Hγ+1)≤C_(τ),where u^(n)and v^(n)denote the numerical solutions at t_(n)=nτ.Moreover,the mass of the numerical solution M(u^(n))satisfies that|M(u^(n))-M(u_0)|≤Cτ~5.

关键词： Davey-StewartsonⅡsystem low-regularity exponential integrator first order accuracy mass conservation

Quinoxaline-specific enantioselective sulfa-michael reaction catalyzed by chiral phosphoric acid

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Chinese Chemical Letters 2023年第12期34卷 172-176页

作者： Xiongfei Deng Shiqi Zhang Hesen Huang Xin cui zhuo Tang Guangxun Li Natural Products Research Center Chengdu Institution of BiologyChinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

Highly enantioselective sulfa-Michael additions(SMA)between 2-alkenyl quinoxalines and aromatic thiols are accomplished using a low loading of chiral phosphoric acid catalyst(1 mol%).It was confirmed by an investigation of a lot of azaarenes that the two C-N units of quinoxalines are indispensable for controlling the reaction enantioselectivities.A series of non-terminal 2-alkenes substituted with aryls or alkyls,even other electro-withdrawing groups such as ketones,esters,or amides,selectively reacted and afforded the desired SMA products(48 examples)in good regioselectivities with high yields(up to 99%)and good ee values(up to 97%).

关键词： Sulfa-Michaela ddition Quinoxaline Chiral phosphoric acid Regioselectivity Enantioselectivity

A549/DDP derived exosomes can affect cisplatin chemosensitivity via transporting CXCR4 to A549 cells

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BIOCELL 2022年第3期46卷 711-720页

作者： MINGMING FANG ning GE JIANFANG LIU YAYUN cui Department of Radiation Oncology Changzhou Cancer Hospital Affiliated to Soochow UniversityChangzhou213032China Department of Cancer Radiotherapy The First Affiliated Hospital of USTCDivision of Life Sciences and MedicineUniversity of Science and Technology of China(Anhui Provincial Cancer Hospital)Hefei230031China Department of Neurology The Affiliated Changzhou No.2 People’s Hospital of Nanjing Medical UniversityChangzhou213001China

The resistance of cancer cells to the anti-cancer drugs is the most important reason that affecting the efficacy of the non-small cell lung cancer(NSCLC)chemotherapy;thus,to explore the underlyingmechanismof drug resistance ofNSCLC medications is urgently needed for improving the therapeutic efficacy of current anti-NSCLC *** aim of the present study is to explore the roles of exosomes in the chemosensitivity of A549 cells and the related mechanism.A549 cells and cisplatin resistant cell line A549/DDP derived exosomes were isolated,and the expressions of CXCR4 were ***,after cisplatin treatment,A549 cells were treated with exosomes,and the proliferation,apoptosis,migration,and invasion of the cells were ***,the tumorigenic effect of A549/DDP derived exosomes were also evaluated by cisplatin treated xenograft tumor mice models in *** found that A549/DDP derived exosomes increased the proliferation,migration,and invasion,and inhibited the apoptosis and cisplatin sensitivity of A549 ***4 was also significantly increased in cells treated with A549/DDP derived ***,A549/DDP derived exosomes may also decrease the chemosensitivity of NSCLC cells to cisplatin in *** data suggested that A549/DDP derived exosomes can affect the chemosensitivity of A549 cells to cisplatin,possibly by transporting CXCR4 to A549 *** data may provide novel evidence for the investigation of drug resistance of NSCLC.

关键词： Exosome Drug resistance CXCR4 NSCLC cells

Evaluation of the in vitro and in vivo Genotoxicity of Almond Skins

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Biomedical and Environmental Sciences 2011年第4期24卷 415-421页

作者： ZHANG XiaoPeng XIANG Qian cui WenMing JIA XuDong LI ning Institute for Nutrition and Food Safety Chinese Center for Disease Control and PreventionBeijing 100021China

Objective It aims to study potential genotoxicity of almond *** A bacterial reverse mutation assay was performed on *** strains TA97,TA98,TA100,TA102,and TA1535 in the absence or presence of S-9 mixture at a dose range of 312.5 to 5 000 μg/plate.A micronucleus test and a mammalian bone marrow chromosome aberration tests were performed in Swiss Albino （CD-1） mice at doses of 625,1 250,and 2 500 mg/kg bw *** Almond skins exerted no mutagenic activity in various bacterial strains of Salmonella typhimurium in either the absence or the presence of metabolic activation at all doses *** doses of almond skins did not affect the proportions of immature to total erythrocytes,the number of micronuclei in the immature erythrocytes,or the number of structural and numerical chromosomal aberrations of Swiss albino *** Almond skins are not genotoxic under the conditions of the in vitro bacterial reverse mutation assay and two in vivo tests-micronucleus test and mammalian bone marrow chromosome aberration test,which supports the safety of almond skins for dietary consumption.

关键词： Almond skins Genotoxicity Mutagenicity Micronuclei Chromosome aberration

Determination of egg and milk allergen in food products by liquid chromatography-tandem mass spectrometry based on signature peptides and isotope-labeled internal standard

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Food Science and Human Wellness 2023年第3期12卷 728-736页

作者： Sufang Fan Junmei Ma zhuo Liu Yawei ning Meicong Cao Qiang Li Yan Zhang Hebei Food Safety Key Laboratory Key Laboratory of Special Food Supervision Technology for State Market RegulationHebei Engineering Research Center for Special Food Safety and HealthHebei Food Inspection and Research InstituteShijiazhuang 050227China College of Bioscience and Bioengineering Hebei University of Science and TechnologyShijiazhuang 050018China

The aim of this work was to develop a liquid chromatography-tandem mass spectrometry method for the determination of milk allergen and egg allergen in food *** peptides GGLEPINFQTAADQAR,VGINYWLAHK,VLVLDTDYK,FFVAPFPEVFGK,and NAVPITPTLNR were confirmed and synthesized as the quantitative peptide of ovalbumin,α-lactalbumin,β-lactoglobulin,α_(S1)-casein andα_(S2)-casein,the relative isotope-labeled internal standards were used in the quantitative *** range was in the range of0.5-5000.0 nmol/L for egg and milk allergen in bread,cake,cookie,rice crust and wheat flour samples with free from egg and milk,the limits of detection of milk allergens and egg allergen were in the range between0.94 mg/100 g and 56.71 mg/100 g,limits of quantification of milk allergens and egg allergen were in the range between 2.36 mg/100 g and 141.78 mg/100 *** recoveries ranged from 76.7%to 122.8%,the relative standard deviations were in the range of 1.60%-15.60%.The developed method has been successfully used for the detection of egg and milk allergen in various food samples.

关键词： Liquid chromatography-tandem mass spectrometry Egg and milk allergen Signature peptides Isotope-labeled internal standards

Nanomedicine-based multimodal therapies:Recent progress and perspectives in colon cancer

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World Journal of Gastroenterology 2023年第4期29卷 670-681页

作者： Yu-Chu He Zi-ning Hao zhuo Li Da-Wei Gao State Key Laboratory of Metastable Materials Science and Technology Nano-Biotechnology Key Laboratory of Hebei ProvinceApplying Chemistry Key Laboratory of Hebei ProvinceYanshan UniversityQinhuangdao 066000Hebei ProvinceChina

Colon cancer has attracted much attention due to its annually increasing *** chemotherapeutic drugs are unsatisfactory in clinical application because of their lack of targeting and severe toxic side *** the past decade,nanomedicines with multimodal therapeutic strategies have shown potential for colon cancer because of their enhanced permeability and retention,high accumulation at tumor sites,co-loading with different drugs,and combination of various *** review summarizes the advances in research on various nanomedicine-based therapeutic strategies including chemotherapy,radiotherapy,phototherapy(photothermal therapy and photodynamic therapy),chemodynamic therapy,gas therapy,and ***,the therapeutic mechanisms,limitations,improvements,and future of the above therapies are discussed.

关键词： Colon cancer Nanomedicine Drug permeability Drug retention Multimodal therapies Therapeutic mechanism

Anti-cd TCR antibody-expanded cd T cells:a better choice for the adoptive immunotherapy of lymphoid malignancies

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Cellular & Molecular Immunology 2012年第1期9卷 34-44页

作者： Jianhua Zhou ning Kang Lianxian cui Denian Ba Wei He Department of Immunology Institute of Basic Medical SciencesChinese Academy of Medical Sciences and School of Basic MedicinePeking Union Medical CollegeNational Key Laboratory of Medical Molecular BiologyBeijingChina

Cell-based immunotherapy for lymphoid malignancies has gained increasing attention as patients develop resistance to conventional *** T cells,which have major histocompatibility complex(MHC)-unrestricted lytic activity,have become a promising candidate population for adoptive cell transfer *** previously established a stable condition for expanding cd T cells by using anti-cd T-cell receptor(TCR)*** this study,we found that adoptive transfer of the expanded cd T cells to Daudi lymphoma-bearing nude mice significantly prolonged the survival time of the mice and improved their living *** further investigated the characteristics of these antibody-expanded cd T cells compared to the more commonly used phosphoantigen-expanded cd T cells and evaluated the feasibility of employing them in the treatment of lymphoid *** but sustained proliferation of human peripheral blood cd T cells was observed upon stimulation with anti-cd TCR *** to phosphoantigen-stimulated cd T cells,the antibody-expanded cells manifested similar functional phenotypes and cytotoxic activity towards lymphoma cell *** is noteworthy that the anti-cd TCR antibody could expand both the Vd1 and Vd2 subsets of cd T *** in vitro-expanded Vd1 T cells displayed comparable tumour cell-killing activity to Vd2 T ***,owing to higher C–C chemokine receptor 4(CCR4)and CCR8 expression,the Vd1 T cells were more prone to infiltrate CCL17-or CCL22-expressing lymphomas than the Vd2 T *** the peripheral blood cd T cells from lymphoma patients further confirmed that the anti-cd TCR antibody-expanded cd T cells could be a more efficacious choice for the treatment of lymphoid malignancies than phosphoantigen-expanded cd T cells.

关键词： adoptive cell therapy anti-cd TCR antibody cd T cells lymphoid malignancies Vd1 subset

A preliminary study on ore-forming environments of Xianglushan-type iron deposit and the weathering mineralization of Emeishan basalt in Guizhou Province, China

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Acta Geochimica 2017年第3期36卷 556-565页

作者： Guofan Cheng Youping Liu Hansheng Long Tao cui ning Yu Guizhou Institute of Tecnology Guiyang 550003 China

Xianglushan-type iron deposits are one of the new types of iron deposits found in the Weining Area of Western Guizhou. The iron-bearing rock system is a paleo-weathered crustal sedimentary(or accumulating) stratum between the top of the Middle-Late Permian Emeishan basalt formation and the Late Permian Xuanwei formation. Iron ore is hosted in the Lower-Middle part of the rock system. In terms of the genesis of mineral deposit, this type of deposit should be a basalt paleo-weathering crustal redeposit type, very different from marine sedimentary iron deposits or continental weathering crust iron deposits. Based on field work and the analytical results of XRD Powder Diffraction, Electron Probe, Scanner Electron Microscope, etc., the geological setting of the ore-forming processes and the deposit features are illustrated in this paper. The ore-forming environment of the deposit and the Emeishan basalt weathering mineralization are also discussed in order to enhance the knowledge of the universality and diversity of mineralization of the Emeishan Large Igneous Province(ELIP), which may be a considerable reference to further research for ELIP metallogenic theories, and geological research for iron deposits in the paleo-weathering crust areas of the Emeishan basalt,Southwestern, China.

关键词： Emeishan basalt paleo-weathering crust Xianglushan-type iron deposit Ore-forming environment Weathering mineralization Western Guizhou Province