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Tailoring of electromagnetic field localizations by twodimensional graphene nanostructures

Light(Science & Applications) 2017年第1期6卷 497-504页

作者： Ze-Bo Zheng jun-Tao Li Teng Ma Han-Lin Fang Wen-Cai Ren jun Chen jun-cong She Yu Zhang Fei Liu Huan-jun Chen Shao-Zhi Deng Ning-Sheng Xu State Key Laboratory of Optoelectronic Materials and Technologies Guangdong Province Key Laboratory of Display Material and TechnologySchool of Electronics and Information TechnologyGuangzhou 510275China State Key Laboratory of Optoelectronic Materials and Technologies School of PhysicsSun Yat-sen UniversityGuangzhou 510275China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China

Graphene has great potential for enhancing light−matter interactions in a two-dimensional regime due to surface plasmons with low loss and strong light *** utilization of graphene in nanophotonics relies on the precise control of light localization ***,we demonstrate the tailoring of electromagnetic field localizations in the mid-infrared region by precisely shaping the graphene into nanostructures with different *** generalize the phenomenological cavity model and employ nanoimaging techniques to quantitatively calculate and experimentally visualize the two-dimensional electromagnetic field distributions within the nanostructures,which indicate that the electromagnetic field can be shaped into specific patterns depending on the shapes and sizes of the ***,we show that the light localization performance can be further improved by reducing the sizes of the nanostructures,where a lateral confinement of λ0/180 of the incidence light can be *** electromagnetic field localizations within a nanostructure with a specific geometry can also be modulated by chemical *** strategies can,in principle,be generalized to other two-dimensional materials,therefore providing new degrees of freedom for designing nanophotonic components capable of tailoring two-dimensional light confinement over a broad wavelength range.

关键词： chemical doping electromagnetic field localizations graphene graphene nanostructures graphene plasmons

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B Cell Epitopes within VP1 of Type O Foot-and-mouth Disease Virus for Detection of Viral Antibodies

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Virologica Sinica 2010年第1期25卷 18-26页

作者： Shan-dian GAO jun-zheng DU Hui-yun CHANG Guo-zheng cong jun-jun SHAO Tong LIN Shuai SONG Qing-ge XIE National Foot-and-mouth Disease Reference Laboratory Key Laboratory of Animal Virology of Ministry of Agriculture State Key Laboratory of Veterinary Etiologieal Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Sciences Lanzhou 730046 China

In this study, the coding region of type O FMDV capsid protein VP1 and a series of codon optimized DNA sequences coding for VP1 amino acid residues 141-160 （epitopel）, tandem repeat 200-213 （epitope2 （＋2）） and the combination of two epitopes （epitopel-2） was genetically cloned into the prokaryotic expression vector PPRoExHTb and pGEX4T-1, respectively. VP1 and the fused epitopes GST-E1, GST-E2 （＋2） and GST-E1-2 were successfully solubly expressed in the cytoplasm of Escherichia coli and Western blot analysis demonstrated they retained antigenicity. Indirect VP1-ELISA and epitope ELISAs were subsequently developed to screen a panel of 80 field pig sera using LPB-ELISA as a standard test. For VP1-ELISA and all the epitope ELISAs, there were clear distinctions between the FMDV-positive and the FMDV-negative samples. Cross-reactions with pig sera positive to the viruses of swine vesicular disease virus that produce clinically indistinguishable syndromes in pigs or guinea pig antisera to FMDV strains of type A, C and Asia1 did not occur. The relative sensitivity and specificity for the GST-E1 ELISA, GST-E2 （＋2）, GST-E1-2 ELISA and VP1-ELISA in comparison with LPB-ELISA were 93.3% and 85.0%, 95.0% and 90%, 100% and 81.8%, 96.6% and 80.9% respectively. This study shows the potential use of the aforementioned epitopes as alternatives to the complex antigens used in current detection for antibody to FMDV structural proteins.

关键词： Foot-and-mouth disease virus ( FMDV) Serology Epitope ELISA

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EXPERIMENT ON THE CHARACTERISTICS OF 3-D VORTEX RING BEHIND A FLEXIBLE OSCILLATING CAUDAL FIN

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Journal of Hydrodynamics 2010年第3期22卷 393-401页

作者： WANG Zhi-dong LAO Yi-jia LI Li-jun cong Wen-chao School of Naval Architecture and Ocean Engineering Jiangsu University of Science and Technology Zhenjiang 212003 China Technology Research Economy Development Institute B eijing 10081 China School of Naval Architecture and Ocean Engineering Jiangsu University of Science and Technology Zhenjiang 212003 China

A test for the wake vortex of a flexible oscillating caudal fin is carried out with Digital Particle Image Velocimetry （DPIV）, and the variation of vortex distance and the vorticity in the range of oscillating frequency from 0.704 Hz to 1.17 Hz are analyzed. It is found that with the increase of the oscillating frequency, the vortex distance decreases and the peak of the vorticity increases, When the Strouhal number is smaller than 0.49, a larger thrust component is obtained. The distribution of the velocity circulation and the vortex distance in the different spanwise section of the caudal fin are given, and then the dimension of the vortex ring is determined. The radius of the vortex ring is 79.3 mm and the average velocity circulation is 28152.9 mm2/s at the oscillating frequency of 0.835 Hz. The model of 3-D vortex ring chain of flexible oscillating caudal fin is constructed based on the information of wake vortex field. Finally, an effective analysis method is provided for establishing the relationship of oscillating parameters for the caudal fin and the wake structure and the intrinsic mechanism of efficient fish swimming is investigated.

关键词： Digital Particle Image Velocimetry (DPIV) flexible caudal fin flow field vortex ring

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Melanoma differentiation-associated gene-7, MDA-7/IL-24, selectively induces growth suppression, apoptosis in human hepatocellular carcinoma cell line HepG2 by replication-incompetent adenovirus vector

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World Journal of Gastroenterology 2006年第11期12卷 1774-1779页

作者： cong-jun Wang Xin-Bo Xue Ji-Lin Yi Kun Chen Jian-WeiZheng Jian Wang Jian-Ping Zeng Rong-Hua Xu Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis in human hepatocellular carcinoma （HCC） cell line HepG2 and normal liver cell line L02. METHODS： We constructed the recombinant replication-incornpetent ***-7 virus vector and infected it into the human HCC cell line HepG2 and normal liver cell line L02. RT-PCR was performed to detect the rnRNA expressing in cells, by ELISA was used to detect MDA-7/IL-24 protein expression in the culture supernatant. The effect of apoptosis induced by ***-7 was confirmed by Hoechst staining and flow cytometry assay with Annexin-V and PI staining. MTT assay was used to determine growth inhibition of HepG2 cells, and cell-cycle and hypodiploidy analyses were performed by flow cytometry. RESULTS： Recombinant replication-defective virus expressing MDA-7/IL-24 was constructed successfully. RTPCR showed that the ***-7 could mediate the expression of the exogenous gene MDA-7/IL-24 into HepG2 and L02. The concentration of MDA-7/IL-24 protein in supernatant was 130 pg/mL and 110 pg/mL in ***-7-infected L02 and HepG2 ceils, respectively. ***-7 infection obviously induced apoptosis （from 2.604±0.72% to 33.64±13.2%, P=0.00012） and growth suppression in HepG2 （inhibition ratio IR=68%） and an increase in the percentage of specific cancer cell types at the G2/M phase of the cell cycle （from 6.44% to 32.29%, P〈 0.01）, but not in L02 ***： These results confirm selectively induction of apoptosis and growth suppression by the mda-7/ IL-24 gene with replication-incompetent adenovirus vector in human hepatocellular carcinoma cell line HepG2.

关键词： Cancer gene therapy Hepatocellular carcinoma (HCC) Apoptosis Growth suppression MDA-7/IL-24

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Adenovirus vector expressing mda-7 selectively kills hepatocellular carcinoma cell line Hep3B

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Hepatobiliary & Pancreatic Diseases International 2008年第5期7卷 509-514页

作者： Xue, Xin-Bo Chen, Kun Wang, cong-jun Zheng, Jian-Wei Yu, Yuan Peng, Zhi-Hai Wu, Zai-De Huazhong Univ Sci & Technol Dept Biliary & Pancreat Surg Tongji Hosp Tongji Med Coll Wuhan 430030 Peoples R China Shanghai Jiao Tong Univ Peoples Hosp Affiliated 1 Dept Gen Surg Shanghai 200030 Peoples R China

BACKGROUND: Melanoma differentiation associated gene-7 (mda-7) is a novel tumor suppressor gene, which has suppressor activity in a broad spectrum of human cancer cells both in vitro and in vivo through activation of various intracellular signaling pathways. In this study, we investigated the potential effect of mda-7 on human hepatocellular carcinoma (HCC) in vitro. METHODS: Cells from the human HCC cell line Hep3B and the human liver cell line L-02 were assigned to three groups. One was cultured in Dulbecco's modified Eagle's medium without serum (control). The others were transfected with adenovirus expressing the mda-7 gene (***-7) or adenovirus vector serving as negative control (***). The expression of MDA-7 and Bcl-2 proteins in Hep3B and L-02 cells was confirmed by the reverse transcriptase-polymerase chain reaction and enzyme-linked immunosorbent assay. The methyl thiazolyl tetrazolium colorimetric assay and flow cytometry were used to assess tumor cell proliferation and the cell cycle. Hoechst and Annexin-V/propidium iodide staining were used to study mda-7 gene expression in Hep3B and L-02 cells. The expression of MDA-7, Bcl-2 and Bax proteins were detected by Western blotting. RESULT S: The mda-7 gene was expressed in Hep3B and L-02 cells. The protein concentrations of MDA-7 in supernatants were 790 and 810 pg/ml, respectively. mda-7 induced Hep3B growth suppression and apoptosis, compared with ***-7 and control (P<0.01). In addition, cell block in G2/M was identified by exposure of HCC cells to secreted MDA-7 protein, but this was not found in L-02. The gene expression of Bcl-2 was markedly decreased in Hep3B but not in L-02. CONCLUSIONS: mda-7 selectively induces growth inhibition and apoptosis in the HCC cell line Hep3B but not in the normal liver cell line L-02 via downregulating the antiapoptosis protein Bcl-2. It could be an ideal gene for gene therapy in HCC.

关键词： replication-incompetent adenovirus vector melanoma differentiation associated-7 gene carcinoma, hepatocellular Bcl-2

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Investigation and reflection on students learning satisfaction in local application-oriented universities

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International Journal of Technology Management 2017年第5期 18-20页

作者： Shan Shan jun cong Harbin University Student Affairs Department 150086 China

In the process of the transformation and development of the local applied undergraduate colleges, how to achieve the goal of cultivating high-quality applied talents is one of the most difficult problems in the sustainable development. The local application of H City University as an example, by using a self-designed questionnaire to investigate the students＇ learning satisfaction, according to the process of the development of the local undergraduate colleges and universities application problems, provide objective basis and reference for the reform and development.

关键词： University Application type Learning satisfaction

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Genomic Sequence Determination of Classical Swine Fever Virus Persistent Infection Strain

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Wuhan University Journal of Natural Sciences 2001年第4期6卷 864-866页

作者： Wu Hai\|xiang, Zhang Chu\|yu , Zheng cong yi, Guo jun qing Institute of Virology, Wuhan University, Wuhan, 430072 Institute of Virology Wuhan University Wuhan

Full genomic sequence of a newly isolated persistent infection strain of classical swine fever virus was firstly determined. It was demonstrated by sequence analyses that nucleotides homologies of this strain compared with virulent Shimen and vaccine HCLV were 89.7%and 87.7%, and homologies of amino acids were 94.8%and 93.3%, respectively. The sequencing results primarily suggest a tighter relationship between this persistent infection strain and virulent Shimen strain than vaccine HCLV strain.

关键词： Classical Swine Fever Virus(CSFV) genomic sequence sequence analysis

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Oncolytic adenovirus-mediated MDA-7/IL-24 overexpression enhances antitumor activity in hepatocellular carcinoma cell lines

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Hepatobiliary & Pancreatic Diseases International 2010年第6期9卷 615-621页

作者： Xiao, Chao-Wen Xue, Xin-Bo Zhang, Hui Gao, Wei Yu, Yuan Chen, Kun Zheng, Jian-Wei Wang, cong-jun Tongji Univ Sch Med Shanghai E Hosp Dept Gen Surg Shanghai 200120 Peoples R China Huazhong Univ Sci & Technol Tongji Med Coll Tongji Hosp Dept Biliary & Pancreat Surg Wuhan 430030 Peoples R China

BACKGROUND: Melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24) is a novel tumor suppressor gene, which has suppressor activity in a broad spectrum of human cancer cells. We investigated the effect of the replication-competent oncolytic adenovirus SG600-IL24 and replication-incompetent adenovirus ***-24, both expressing human MDA-7/IL-24 on the hepatocellular carcinoma cell lines HepG2, Hep3B, SMMC-7721, HCCLM3, and the normal liver cell line L02. METHODS: Hepatocellular carcinoma cell lines and the normal liver cell line were infected with SG600-IL24 and ***-24. The mRNA and protein expression of MDA-7/IL-24 in infected cells was confirmed by RT-PCR, ELISA, and Western blotting. MTT assay was used to investigate the proliferation effect. Hoechst staining and Annexin-V and PI staining were performed to study the MDA-7/IL-24 gene expressed in HCC cell lines and the normal liver cell line. Flow cytometry was used to analyse the cell cycle. RESULTS: RT-PCR, ELISA and Western blotting confirmed that the exogenous MDA-7/IL-24 gene was highly expressed in cells infected with SG600-IL24. MTT and apoptosis detection indicated that SG600-IL24 induced growth suppression, promoted apoptosis, and blocked cancer cell lines in the G2/M phase in hepatocellular carcinoma cell lines but not in the normal liver cell line. CONCLUSIONS: SG600-IL24 selectively induces growth suppression and apoptosis in hepatocellular carcinoma cell lines in vitro but not in the normal liver cell line L02. Compared with ***-24, SG600-IL24 dramatically enhances antitumor activity in hepatocellular carcinoma cell lines. (Hepatobiliary Pancreat Dis Int 2010; 9:615-621)

关键词： melanoma differentiation-associated gene-7 interleukin-24 oncolytic adenovirus hepatocellular carcinoma gene therapy

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Athermal design of the mirror support with flexure hinges for the laser communication terminal

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Optoelectronics Letters 2019年第6期15卷 454-458页

作者： YANG Feng-fu TIAN Hai-ying YAN Chang-xiang WU cong-jun MU De-qiang Changchun Institute of Optics Fine Mechanics and PhysicsChinese Academy of SciencesChangchun 130033China University of Chinese Academy of Sciences Beijing 100049China Center of Materials Science and Optoelectrics Engineering University of Chinese Academy of ScienceBeijing 100049China Changchun University of Technology Changchun 130012China

In order to suppress the effect of the temperature variation on the wavefront of the laser communication terminal, the secondary mirror support with flexure hinges is designed. The series-wound straight-circle flexure hinge is designed to achieve the maximal variation range of the flexibility or stiffness with the limit of flexure hinges' geometrical size. The position and quantity of the flexure hinges are determined to control the deformation direction of the secondary mirror. In order to search the direction in which the wavefront aberration is minimum, the flexure hinges' parameters are optimized with the system wavefront aberration as the optimization objective. The prototype of the laser communication terminal is constructed and the value of the wavefront aberration is measured under the condition of 20±2°C. Experimental results show that the value of the wavefront aberration root mean square(RMS) is reduced from 0.066λ to 0.042λ, meeting the requirement of RMS less than 1/20λ(λ=632.8 nm). The athermal design method presented in this paper provides a novel way for the athermal design of the small aperture mirror support in off-axis optical systems.

关键词： communication laser mirror

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Differential analysis of intestinal microbiota and metabolites in mice with dextran sulfate sodium-induced colitis

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World Journal of Gastroenterology 2022年第43期28卷 6109-6130页

作者： Jia-Li Wang Xiao Han jun-Xiang Li Rui Shi Lei-Lei Liu Kai Wang Yu-Ting Liao Hui Jiang Yang Zhang jun-cong Hu Li-Ming Zhang Lei Shi Graduate School Beijing University of Chinese MedicineBeijing 100029China Department of Gastroenterology Dongfang HospitalBeijing University of Chinese MedicineBeijing 100078China College of Traditional Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China Department of Emergency The First Teaching Hospital of Tianjin University of Traditional Chinese MedicineTianjin 300193China Department of Geriatrics Gulou Hospital of Traditional Chinese MedicineBeijing 100009China

BACKGROUND Intestinal micro-ecological imbalances impair the intestinal barrier and induce intestinal inflammation,for example,ulcerative colitis(UC).According to the latest research,abnormalities in intestinal microbiota structure and their metabolites play a dominant role in UC progression;in addition,they could affect the mucus barrier based on different *** numerous studies have confirmed the important role of intestinal microbiota in UC pathogenesis,the intricate connection between microbiota and metabolites and mucus barrier in UC occurrence remains unclear,and correlation analyses of differential microbiota and their metabolites under UC are relatively *** To reveal the differential intestinal microbiota and metabolites in UC pathogenesis and explore more sensitive biomarker *** We used the antibiotic combination method to establish intestinal pseudo-aseptic mice;afterward,dextran sulfate sodium(DSS)was applied to establish an acute experimental colitis mice *** severity,assessed based on disease activity index,colorectal length,colorectal wet weight,and histological lesions,and mucus-related staining(mucopolysaccharide alcian blue and immunofluorescence of mucin),was compared between the pseudo-aseptic and bacterial colitis ***,differential intestinal microbiota,metabolites,and their association and correlations,were analyzed by 16s rDNA sequencing in combination with non-targeted metabolomics,through gas chromatography-mass *** Compared with the pseudo-aseptic mice,intestinal bacteria positive mice were more severely ill and their intestinal mucus loss was more pronounced in DSS-induced colitis(P<0.05),suggesting that different microbiota and metabolites could cause the different degrees of ***,we observed that in addition to Klebsiella,and Bacteroides,which were widely associated with colitis,Candidatus Stoquefichus,Anaerobiospirillum,Muribaculum,and Negativibacillus

关键词： Ulcerative colitis Gut microbiota Metabolites Dextran sulfate sodium Mucin

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