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Rapid,specific and sensitive detection of Vibrio vulnificus by loop-mediated isothermal amplification targeted to vvhA gene

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Acta Oceanologica Sinica 2018年第4期37卷 83-88页

作者： ZHANG Lina WANG Mingyi cong dianxia DING Shuyan cong Rinan YUE Jinyong GENG Jianli HU Chengjin Department of Laboratory Medicine General Hospital of Jinan Military Region of PLA Department of Central Laboratory Weihai Municipal Hospital affiliated to Dalian Medical University

Vibrio vulnificus is an estuarine bacterial pathogen for *** rapid,specific and sensitive detection of *** is urgently needed for early disease diagnosis and timely treatment of *** *** the study,a loop-mediated isothermal amplification（LAMP） technique was developed for *** detection with a set of primers,composed of two out primers and two inner primers targeted to vvh A *** optimal amplification temperature was 63°C and the reaction only took 35 *** amplification products could not only be detected by agarose gel electrophoresis with ladder-like pattern bands,but also could be visualized using calcein with naked eye ***-five strains were tested for the specificity of LAMP assay,and all the *** strains were identified correctly while other strains were negative *** sensitive of the new LAMP assay was 100-fold more sensitive than the conventional ***,all the *** strains were detected correctly in spiked,clinical and environmental samples by the new LAMP *** with other well-known techniques,the new LAMP assay targeted to vvh A gene was extremely rapid,simple,sensitive and specific for *** identification.

关键词： Vibrio uulnificus loop-mediated isothermal amplification vvhA gene

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