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Development of hybrid-type modified chitosan derivative nanoparticles for the intracellular delivery of midkine-siR NA in hepatocellular carcinoma cells

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Hepatobiliary & Pancreatic Diseases International 2015年第1期14卷 82-89页

作者： Jing Zhong Hui-lian Huang Jing Li Fu-chu Qian Li-Qin Li Ping-Ping Niu Li-Cheng Dai Huzhou Key Laboratory of Molecular Medicine Huzhou Central Hospital

BACKGROUND： Hepatocellular carcinoma（HCC） is one of the most common cancers worldwide. Most of the patients with HCC lose the surgical opportunity at the time of diagno sis. Some novel therapeutic modalities, like gene therapy, are promising for the treatment of HCC. However, the success of gene therapy depends on two aspects： efficient gene materials and gene delivery vectors. The present study was to develop new chitosan-based nanoparticles for a midkine-si RNA（anti HCC gene drug） ***： The novel gene delivery vector（MixN CH） was syn thesized by hybrid-type modification of chitosan with 2-chloro ethylamine hydrochloride and N, N-dimethyl-2-chloroethylamine hydrochloride. The chemical structure of Mix NCH was char acterized by FT-IR and 1HNMR. The cytotoxicity of Mix NCH was determined by MTS assay. The gene condensation ability and size, zeta potential and morphology of Mix NCH/MK si RNA nanoparticles were measured. The in vitro transfection and gene knockdown efficiency of midkine by Mix NCH/MK si RNA nanoparticles was detected by q RT-PCR and Western blotting. Gene knockdown effect at the molecule level on the proliferation of Hep G2 in vitro was determined by MTS ***： Mix NCH was successfully acquired by aminoalkyl ation modification of chitosan. The Mix NCH could condense MK-siR NA well above the weight ratio of 3. The average size of Mix NCH/MK-si RNA nanoparticles was 100-200 nm, and thesurface charge was about ＋5 m V. Morphologically, Mix NCH/MK-si RNA nanoparticles were in regular spherical shape-with no aggregation. Regarding to the in vitro transfection of nanoparticles, the MixN CH/MK-siR NA nanoparticles reduced MK m RNA level to 14.03%±4.03%, which were comparable to Biotrans（8.94%±3.77%）. Mix NCH/MK-si RNA effectively inhibited the proliferation of Hep G2 in vitro.-CONCLUSION： Mix NCH/MK-si RNA nanoparticles could be effective for the treatment of hepatocellular carcinoma.

关键词： chitosan gene vector nanoparticles midkine siRNA hepatocellular carcinoma

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Efficient and robust missing key tag identification for large-scale RFID systems

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Digital Communications and Networks 2023年第6期9卷 1421-1433页

作者： chu chu Guangjun Wen Jianyu Niu School of Information and Communication Engineering University of Electronic Science and Technology of ChinaChengdu611731China Research Institute of Trustworthy Autonomous Systems Southern University of Science and TechnologyShenZhen518055China School of Engineering The University of British ColumbiaKelownaV1V 1V7BCCanada

Radio Frequency Identification(RFID)technology has been widely used to identify missing *** many applications,rapidly pinpointing key tags that are attached to favorable or valuable items is *** realize this goal,interference from ordinary tags should be avoided,while key tags should be efficiently *** many previous studies,how to rapidly and dynamically filter out ordinary tags when the ratio of ordinary tags changes has not been ***,how to efficiently verify missing key tags in groups rather than one by one has not been explored,especially with varying missing *** this paper,we propose an Efficient and Robust missing Key tag Identification(ERKI)protocol that consists of a filtering mechanism and a verification ***,the filtering mechanism adopts the Bloom filter to quickly filter out ordinary tags and uses the labeling vector to optimize the Bloom filter's performance when the key tag ratio is ***,the verification mechanism can dynamically verify key tags according to the missing rates,in which an appropriate number of key tags is mapped to a slot and verified at ***,we theoretically analyze the parameters of the ERKI protocol to minimize its execution *** numerical results show that ERKI can accelerate the execution time by more than 2.14compared with state-of-the-art solutions.

关键词： RFID Missing key tag identification Time efficiency Robust

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A-type granites induced by a breaking-off and delamination of the subducted Junggar oceanic plate,West Junggar,Northwest China

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China Geology 2022年第3期5卷 457-474页

作者： chu Wu Tao Hong Xing-Wang Xu Cheng-Xi Wang lian-Hui Dong Development and Research Center of China Geological Survey Ministry of Natural ResourcesBeijing 100037China Guangdong Provincial Key Lab of Geodynamics and Geohazards School of Earth Sciences and EngineeringSun Yat-sen UniversityGuangzhou 510275China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai 519082China Key Laboratory of Mineral Resources Institute of Geology and GeophysicsChinese Academy of SciencesBeijing 100029China University of Chinese Academy of Sciences Beijing 100049China Institutions of Earth Science Chinese Academy of SciencesBeijing 100029China Xinjiang Bureau of Geology and Mineral Resources UrumqiXinjiang 830000China

The A-type granites with highly positiveε_(Nd)(t)values in the West Junggar,Central Asian Orogenic Belt(CAOB),have long been perceived as a group formed under the same tectonic and geodynamic setting,magmatic sourceq and petrogenetic *** evidence shows that these granites occurred at two different tectonic units related to the southeastern subduction of Junggar oceanic plate:the Hongshan and Karamay granites emplaced in the southeast of West Junggar in the Baogutu continental arc;whereas the Akebasitao and Miaoergou granites formed in the accretionary *** the authors present new bulk-rock geochemistry and Sr-Nd isotopes,zircon U-Pb ages and Hf-O isotopes data on these *** granites in the Baogutu continental arc and accretionary prism contain similar zirconε_(Hf)(t)values(+10.9 to+16.2)and bulk-rock geochemical characteristics(high SiO_(2)and K_(2)O contents,enriched LILEs(except Sr),depleted Sr,Ta and Ti,and negative anomalies in Ce and Eu).The Hongshan and Karamay granites in the Baogutu continental arc have older zircon U-Pb ages(315-305 Ma)and moderate^(18)O enrichments(δ^(18)_(O_(zircon))=+6.41‰-+7.96‰);whereas the Akebasitao and Miaoergou granites in the accretionary prism have younger zircon U-Pb ages(305-301 Ma)with higher^(18)O enrichments(δ^(18)_(O_(zircon))=+8.72‰-+9.89‰).The authors deduce that the elevated^(18)O enrichments of the Akebasitao and Miaoergou granites were probably inherited from low-temperature altered oceanic *** Akebasitao and Miaoergou granites were originated from partial melting of low-temperature altered oceanic crusts with juvenile oceanic sediments below the accretionary *** Hongshan and Karamay granites were mainly derived from partial melting of basaltic juvenile lower crust with mixtures of potentially chemical weathered ancient crustal residues and mantle basaltic melt(induced by hot intruding mantle basaltic magma at the bottom of the Baogutu continental arc).On the other hand,the Miaoe

关键词： A-type granite plutons Sr-Nd-Hf-O isotopes Breaking-off and delamination Subducted Junggar oceanic plate West Junggar Central Asian Orogenic Belt

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Concurrent alcoholic cirrhosis and malignant peritoneal mesothelioma in a patient:A case report

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World Journal of Clinical Cases 2022年第19期10卷 6716-6721页

作者： liang Liu Xiao-Yan Zhu Wen-Jie Zong chuan-lian chu Jing-Yu Zhu Xing-Jie Shen Department of Gastroenterology Central Hospital Affiliated to Shandong First Medical UniversityJinan 250011Shandong ProvinceChina Department of Gastroenterology Jinan Clinical Research Center for Digestive DiseasesJinan 250011Shandong ProvinceChina

BACKGROUND Malignant peritoneal mesothelioma(MPM)originates from the mesothelial and subcutaneous cells of the abdominal *** diagnose is difficult due to its nonspecific and vague symptoms,and it should be differentiated from alcoholic cirrhosis and liver and pancreatic *** and missed diagnosis can easily occur when MPM presents with other *** the best of our knowledge,no case of MPM concurrent with alcoholic cirrhosis has been *** SUMMARY A 63-year-old man presented to our hospital with abdominal distension for *** had a history of alcohol consumption for nearly 30 years and no history of special drug use or toxic *** treatment for alcoholic cirrhosis in a community hospital,his symptoms did not improve *** patient underwent exploratory laparotomy and surgical *** examination showed an epithelioid *** was treated with chemotherapy and intraperitoneal hyperthermic perfusion after ***,he is in a stable condition and tumor recurrence has not *** Misdiagnosis and missed diagnosis of MPM can easily occur because of its insidious ***,there is a need to *** in clinical practice,make the correct diagnosis,and provide timely and effective treatment.

关键词： Malignant peritoneal mesothelioma Abdominal distension Ascites Cirrhosis Computed tomography Case report

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Soil CO_2 and N_2O Emissions in Maize Growing Season Under Different Fertilizer Regimes in an Upland Red Soil Region of South China

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Journal of Integrative Agriculture 2014年第3期13卷 604-614页

作者： ZHANG Xu-bo WU lian-hai SUN Nan DING Xue-shan LI Jian-wei WANG Bo-ren LI Dong-chu Key Laboratory of Crop Nutrition and Fertilization Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning Chinese Academy of Agricultural Sciences Sustainable Soils and Grassland Systems Department Rothamsted Research Department of Botany and Microbiology University of Oklahoma

Upland red soils have been identified as major CO2 and N2O sources induced by human activities such as fertilization. To monitor characteristics of soil surface CO2 and N2O fluxes in cropland ecosystems after continuous fertilizer applications over decades and to separate the respective contributions of root and heterotrophic respiration to the total soil CO2 and N2O fluxes, the measurements of soil surface CO2 and N2O fluxes throughout the maize growing season in 2009 were carried out based on a fertilization experiment （from 1990） through of the maize （Zea mays L.） growing season in red soil in southern China. Five fertilization treatments were chosen from the experiment for study： zero-fertilizer application （CK）, nitrogen-phosphorus- potassium （NPK） fertilizer application only, pig manure （M）, NPK plus pig manure （NPKM） and NPK with straw （NPKS）. Six chambers were installed in each plot. Three of them are in the inter-row soil （NR） and the others are in the soil within the row （R）. Each fertilizer treatment received the same amount of N （300 kg ha-1 yr-1）. Results showed that cumulative soil CO2 fluxes in NR or R were both following the order： NPKS〉M, NPKM〉NPK〉CK. The contributions of root respiration to soil CO2 fluxes was 40, 44, 50, 47 and 35% in CK, NPK, NPKM, M and NPKS treatments, respectively, with the mean value of 43%. Cumulative soil N2O fluxes in NR or R were both following the order： NPKS, NPKM〉M〉NPK〉CK, and soil N2O fluxes in R were 18, 20 and 30% higher than that in NR in NPKM, M and NPKS treatments, respectively, but with no difference between NR and R in NPK treatment. Furthermore, combine with soil temperature at -5 cm depth and soil moisWxe （0-20 cm） together could explain 55-70% and 42-59% of soil CO2 and N2O emissions with root interference and 62- 78% and 44-63% of that without root interference, respectively. In addition, soil CO2 and N2O flUXeS per unit yield in NPKM （0.55 and 0.10 kg C t^-1） and M （0.65 and 0.13 g N t^-1） treatments were

关键词： greenhouse gas emissions carbon dioxide nitrous oxide manure mineral fertilizers straw return South China

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电动拖拉机双电源并联控制研究

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农机化研究 2025年第1期47卷 234-238页

作者：初香港王宝超陈叶青岛农业大学机电工程学院山东青岛266109

电动拖拉机具有结构简单和节能减排等优点,但现有电动拖拉机作业时采用单一动力电池能源,存在续航时间短的问题。为了提高电动拖拉机长续航供电能力,提出了一种基于下垂控制的直流双电源并联均流联合供电方案,利用MatLab/Simulink搭建... 详细信息

电动拖拉机具有结构简单和节能减排等优点,但现有电动拖拉机作业时采用单一动力电池能源,存在续航时间短的问题。为了提高电动拖拉机长续航供电能力,提出了一种基于下垂控制的直流双电源并联均流联合供电方案,利用MatLab/Simulink搭建了系统模型。仿真试验表明:单电源可以实现升压稳压控制;基于下垂控制的双电源并联均流能够实现双电源的电流均流;当切换不同功率负载时,系统电压随功率增加按比例下调,验证了该方案的可行性。

关键词：电动拖拉机双电源并联下垂控制动态仿真

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Analysis of effect factors-based stochastic network planning model

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Journal of Systems Engineering and Electronics 2008年第1期19卷 112-118页

作者： chu chunchao China Academy of Transportation Science Beijing 100029 P. R. China

Looking at all the indeterminate factors as a whole and regarding activity durations as independent random variables, the traditional stochastic network planning models ignore the inevitable relationship and dependence among activity durations when more than one activity is possibly affected by the same indeterminate factors. On this basis of analysis of indeterminate effect factors of durations, the effect factors-based stochastic network planning （EFBSNP） model is proposed, which emphasizes on the effects of not only logistic and organizational relationships, but also the dependent relationships, due to indeterminate factors among activity durations on the project period. By virtue of indeterminate factor analysis the model extracts and describes the quantitatively indeterminate effect factors, and then takes into account the indeterminate factors effect schedule by using the Monte Carlo simulation technique. The method is flexible enough to deal with effect factors and is coincident with practice. A software has been developed to simplify the model-based calculation, in *** language. Finally, a case study is included to demonstrate the applicability of the proposed model and comparison is made with some advantages over the existing models.

关键词： stochastic network planning activity duration effect factor

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An eight-year journey of Hepatobiliary & Pancreatic Diseases International

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Hepatobiliary & Pancreatic Diseases International 2010年第5期9卷 458-459页

作者： Lei, shui-Ying Wang, Pan-Zhi Dong, Yan-Ping Zhu, Cong Qian, Shou-chu Zheng, Shu-Sen Zhejiang Univ Sch Med Affiliated Hosp 1 Hangzhou 310003 Zhejiang Peoples R China

History of the journal Founded in 2002 as a quarterly in English, Hepatobiliary & Pancreatic Diseases International (HBPD INT) has since 2007 been publishing bimonthly. Owned

关键词： In An eight-year journey of Hepatobiliary Pancreatic Diseases International

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滇东北地区滑坡易发性评价

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地震研究 2025年第1期48卷 89-99页

作者：刘绍昌白仙富储正伟云南省地震局云南昆明650224 云南南方地勘工程有限公司云南大理671000

在研究滇东北地震滑坡危险区滑坡致灾因子和孕灾环境的基础上,选取了地形、地质、其他共3大类12项评价因子,通过评价因子独立性检验,构建滑坡易发性评价指标体系。随机选取研究区70%的滑坡点作为滑坡易发性评价训练样本,运用GIS空间分... 详细信息

在研究滇东北地震滑坡危险区滑坡致灾因子和孕灾环境的基础上,选取了地形、地质、其他共3大类12项评价因子,通过评价因子独立性检验,构建滑坡易发性评价指标体系。随机选取研究区70%的滑坡点作为滑坡易发性评价训练样本,运用GIS空间分析功能,分别采用确定性系数模型、二元逻辑回归模型以及二者组成的耦合模型进行滑坡易发性评价。选取未参与训练样本的30%滑坡点作为检验样本,使用受试者工作特征(ROC)曲线对以上3种模型的评价结果和精度进行验证。结果显示:高程、坡度、距道路距离、地质岩性、NDVI、年平均降雨量对滑坡易发性影响相对较大。耦合模型的AUC值为0.781,高于确定性系数模型的AUC值0.754和二元逻辑回归模型的AUC值0.748。耦合模型合理性和评价精度高于单一模型,且均符合模型检验要求。耦合模型揭示了各评价因子对滑坡易发性影响的差异性,能够较好地反映滑坡易发性影响因子之间相互权重大小,预测的研究区滑坡的空间分布与野外实际调查结果高度吻合。

关键词：滇东北滑坡易发性耦合模型

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Angiogenesis and Hepatic Fibrosis： Western and Chinese Medicine Therapies on the Road

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Chinese Journal of Integrative Medicine 2018年第9期24卷 713-720页

作者： WANG Jing-si ZHANG Qiu-yun CHENG Jin-lian CHEN Lan-yu YAO Nai-li SUN Gui-zhi chu Yu-ling Department of Hepatology Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University Beijing 2100010 China College of Traditional Chinese Medicine Affiliated to Capital Medical University Beijing 2100069 China Department of Good Clinical Practice Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University Beijing 2100010 China Department of Hepatology Guang'anmen Hospital of China Academy of Chinese Medical Sciences Beijing 2100053 China Department of Gastroenterology Guang'anmen Hospital of China Academy of Chinese Medical Sciences Beijing 2100053 China Department of Oncology Guang'anmen Hospital of China Academy of Chinese Medical Sciences Beijing 2100053 China

Hepatic fibrosis is a common feature of almost all chronic liver diseases. Formation of new vessels （angiogenesis） is a process strictly related to the progressive fibrogenesis which leads to cirrhosis and liver cancer. This review mainly concerns the relationship between angiogenesis and hepatic fibrosis, by considering the mechanism of angiogenesis, cells in angiogenesis, anti-angiogenic and Chinese medicine therapies.

关键词： angiogenesis hepatic fibrosis review Chinese medicine

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