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Reversal of multi-drug resistance by pSUPER-shRNA-mdr1 in vivo and in vitro

World Journal of Gastroenterology 2009年第4期15卷 431-440页

作者： guang-Dong Pan Jian-Qing Yang Lv-Nan Yan guang-Ping chu Qiang Liu Yi Xiao Lin Yuan Department of General Surgery People's Hospital of Liuzhou Medical Universityof Guangxi Liuzhou 545001 Guangxi Zhuang Autonomous Region China

AIM： To explore the possibility of reversing multi-drug resistance （MDR） to HepG2/mdr1 in vitro and in vivo with RNA interference （RNAi）. METHODS： HepG2/mdr1 was obtained by cloning the whole gene mdrl into HepG2 cells, shRNA targeting sequence was designed to be homologous to the P-gp encoding MDR1 mRNA consensus sequence, pSUPER- shRNA/mdrl was constructed using the enzyme- digested technique. HepG2/mdrl cells were transfected with vectors of pSUPER-shRNA/mdrl to measure their efficacy by real-time PCR for mdrl mRNA, flow cytometry （FCM） for P-gp expression, and Rhodamine efflux, MTT method for HepG2/mdrl function, respectively. In vivo, mice tumors were treated by injecting pSUPER-shRNA/mdrl in situ and into intra- abdominal cavity. Tumors were collected to create cell suspension and cryosections after chemothearpy with adiramycin and mytomycin. The cell suspension was incubated in RPMI-1640 supplemented with G418 to screen stable cells for appreciating the reversal of MDR. Cryosections were treated with immunohistochemistry technique to show the effectiveness of transfection and the expression of ***： pSUPER-shRNA/mdrl was successfully constructed, which was confirmed by sequencing. The MDR phenotype of HepG2/mdr1 was decreased significantly in vitro transfection. HepG2/mdr1 showing its MDR was reversed notably in P-gp expression （11.0% vs 98.2%, P 〈 0.01）. Real-time PCR showed that mRNA/mdrl was lower in test groups than in control groups （18.73± 1.33 vs 68.03±2.21, P 〈 0.001）. Compared with HepG2, the sensitivity of HepG2/ mdrl and HepG2/mdr1-dsRNA cells to ADM was decreased by 1.64 times and 15.6 times, respectively. The accumulation of DNR in positive groups was decreased evidently. In vivo, the p-gp expression in positive groups was significantly lower than that in control groups （65.1% vs 94.1%, P 〈 0.05）. The tumor suppressing rate in test groups was 57.8%. After chemotherapy, the growth rate in test groups was lower than that in control groups （

关键词： Hepatocellular carcinoma Multi-drugresistance RNA interference

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Improved method for zero-energy mode suppression in peridynamic correspondence model

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Acta Mechanica Sinica 2019年第5期35卷 1021-1032页

作者： Ji Wan Zhuang chen Xihua chu Hui Liu Department of Engineering Mechanics Wuhan UniversityWuhan 430072China

The peridynamic correspondence model provides a general formulation to incorporate the classical local model and,therefore,helps to solve mechanical problems with discontinuities *** it suffers from zero-energy mode instability in numerical implementation due to the approximation of deformation gradient *** suppress zero-energy modes,previous stabilized methods were generally more based on adding a supplemental force state derived from bond-based peridynamic theory,which requires a bond-based peridynamic *** this work,we present an improved stabilized method where the stabilization force state is derived directly from the peridynamic correspondence ***,the bond-based peridynamic micro-modulus is *** improved method needs no extra constant to control the magnitude of stabilization force state and it is suitable for either isotropic or anisotropic *** examples are presented to demonstrate its performance in simulating crack propagation,and numerical results show its efficiency and effectiveness.

关键词： Peridynamic correspondence model Zero-energy modes Anisotropic material Damage

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NUMERICAL SIMULATION OF TURBULENCE AND ITS STRUCTURE IN A HYDROCYCLONE

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中国有色金属学会会刊：英文版 1999年第1期9卷 130-136,132+134+136+138+页

作者： chu, Liangyin chen, Wenmei Li, Xiaozhong Wu, Chigong Sichuan Union Univ Chengdu China

１ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｓｔｒｕｃｔｕｒｅｏｆｔｕｒｂｕｌｅｎｃｅｉｎｈｙｄｒｏｃｙｃｌｏｎｅｓｈａｓａｎｉｎｆｌｕｅｎｃｅｎｏｔｏｎｌｙｏｎｔｈｅｓｅｐａｒａｔｉｏｎｐｅｒｆｏｒｍａｎｃｅｂｕｔａｌｓｏｏ... 详细信息

１ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｓｔｒｕｃｔｕｒｅｏｆｔｕｒｂｕｌｅｎｃｅｉｎｈｙｄｒｏｃｙｃｌｏｎｅｓｈａｓａｎｉｎｆｌｕｅｎｃｅｎｏｔｏｎｌｙｏｎｔｈｅｓｅｐａｒａｔｉｏｎｐｅｒｆｏｒｍａｎｃｅｂｕｔａｌｓｏｏｎｔｈｅｅｎｅｒｇｙｃｏｎｓｕｍｐ...

关键词： hydrocyclone numerical simulation of turbulence turbulence structure

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Behavior of Liquid Steel in Mold for Thin Slab Caster

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Journal of Iron and Steel Research International 2003年第4期10卷 18-22页

作者： WEN guang-hua TANG Ping HE Jun-fan XU chu-shao Chongqing University

The comparison of different submerged entry nozzles(SEN)was made by numerical simulation regarding the influence of liquid steel flow field,heat transfer and mold fluxes,as well as solidification shell stress and surface quality of thin *** optimum internal shape of mold and the SEN were *** foundation for choice of internal shape and casting parameters of mold was built.

关键词： thin slab continuous casting mold submerged entry nozzle liquid steel

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Thioetherification of isoprene and butanethiol on transition metal phosphides

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Journal of Energy Chemistry 2018年第3期27卷 930-939页

作者： Tianyu Ren Mingfeng Li Yang chu Jixiang chen Tianjin Key Laboratory of Applied Catalysis Science and Technology Department of Catalysis Science and Engineering School of Chemical Engineering and Technology Tianjin University Research Institute of Petroleum Processing SINOPEC

Thioetherification between mercaptan and diolefin is an efficient process to remove mercaptans in FCC gasoline at mild condition, during which the selective hydrogenation of diolefin to monoolefin is also expected. Here, Si O2 supported transition metal（Fe, Co, Ni, Mo and W） phosphides were tested for the thioetherification of isoprene and butanethiol on a fixed-bed reactor at 120℃ and 1.5 MPa H2, and their structure before and after reaction was characterized by means of XRD, HRTEM, N2 sorption, CO chemisorption, NH3-TPD, XPS and TG. It was found that, among different metal phosphides, Mo P/Si O2 showed the best performance, and the optimal nominal Mo P loading was 25%. Apart from the nature of metal, the density of metal and acid sites determined the catalyst performance. Metal site was mainly responsible for hydrogenation of isoprene, while acid site dominantly contributed to the thioetherification and the polymerization of olefins. Moreover, a balance between metallic and acidic functions is required to arrive at a desired performance. Excessive metal sites or acid sites led to the over-hydrogenation of isoprene or the severe polymerization of olefins, respectively. 25%Mo P/Si O2 was tested for 37 h time on stream, and butanethiol conversion maintained at 100%; although isoprene conversion remarkably decreased, the selectivity to isopentenes exceeded 80% after reaction for 11 h. We suggest that the deactivation of Mo P/Si O2 is mainly ascribed to the butanethiol poisoning and the carbonaceous deposit, especially the former.

关键词： Transition metal phosphides Bifunctional catalyst Thioetherification Selective hydrogenation Catalyst deactivation

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High Selectivity Cascaded Quadruplet Bandpass Filter with Three Pairs of Transmission Zeros

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Chinese Journal of Electronics 2017年第5期26卷 1101-1104页

作者： chen Jianfeng chen Fuchang LI Runshuo XIE Zeming chu Qingxin School of Electronic and Information Engineering South China University of Technology

A novel microstrip Cascaded quadruplet(CQ) bandpass filter using quarter wavelength resonators and three-quarter wavelength resonators is *** on cross coupling and source-load coupling,three pairs of finite transmission zeros are generated at the *** a result,the out-of-band rejection can be enhanced and high selectivity is *** on the proposed structure,a cascaded quadruplet filter centering at 3.0GHz is designed and *** agreement between the measured and simulated data is obtained.

关键词： Cascaded quadruplet(CQ) Bandpass filter Cross coupling Transmission zeros Microstrip

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Microstructure and Mechanical Behavior of FeNiCoCr and FeNiCoCrMn High-Entropy Alloys Fabricated by Powder Metallurgy

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Acta Metallurgica Sinica(English Letters) 2021年第4期34卷 445-454页

作者： chenliang chu Weiping chen Zhen chen Zhenfei Jiang Hao Wang Zhiqiang Fu guangdong Key Laboratory for Advanced Metallic Materials Processing South China University of TechnologyGuangzhou 510640China Shantou Technician College Shantou 515041China

The present work reports a systematic investigation on phase evolution,microstructure and microstructure-property relationship of two typical face-centered cubic(FCC)structured high-entropy alloys(HEAs),FeNiCoCr and FeNiCoCrMn,prepared via mechanical alloying(MA)followed by spark plasma sintering(SPS).Following 50 h of MA,the two HEAs consisted of a mixture of FCC and body-centered cubic *** SPS,the bulk FeNiCoCr alloy showed a primary FCC phase with a small amount of Cr_(23)C_(6) and Cr_(2)O_(3) contaminants,while the bulk FeNiCoCrMn alloy was composed of a primary FCC phase with some(Cr,Mn)23C6 and MnCr_(2)O_(4) *** average grain size of the primary FCC phase in the bulk FeNiCoCr alloy was~416 nm,while that of the primary FCC phase in the bulk FeNiCoCrMn alloy was~547 *** yield strength,compressive strength and strain-to-failure of the bulk FeNiCoCr alloy are 1525 MPa,1987 MPa and 24.4%,respectively,whereas those of the bulk FeNiCoCrMn alloy are 1329 MPa,1761 MPa and 21.9%,*** suggests that the bulk FeNiCoCrMn exhibited lower strength and plasticity in comparison with the bulk FeNiCoCr ***,the smaller grain size of the primary FCC phase in the FeNiCoCr alloy is mainly responsible for the better mechanical performance.

关键词： High-entropy alloy Mechanical alloying Microstructure Strengthening Contaminants

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Model of the Crustal Magnetic Field in the Martian Aurora Zone

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Chinese Physics Letters 2011年第11期28卷 286-288页

作者： TONG Dong-Sheng chen chu-Xin School of Earth and Space Sciences University of Science and Technology of ChinaHefei 230026

It is well known that aurorae are prominent on planets with a global magnetic field and occur where open magnetic field lines *** UV spectrometer used for investigating the characteristics of the atmosphere of Mars(SPICAM)on board the Mars Express made the first observation of auroral-type emission in the cusp region of the strong crustal magnetic field on Mars and found that the arc of the Martian aurora zone is very narrow in width,which obviously differs from that of other *** on the observation,we put forward a model of a crustal magnetic field on the Martian aurora zone through the morphology of Martian *** the model,equivalent currents are proposed;the topology and magnitude of the magnetic field generated by these equivalent currents are consistent with that of the crustal magnetic field in the Martian aurora *** morphology of the Martian aurora zone generated through the model matches well with the observations made by the Mars Express orbiter.

关键词： zone crustal currents

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Selective capture of Tl2O from flue gas with formation of p-n junction on V_(2)O_(5)-WO_(3)/TiO_(2)catalyst under working conditions

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Green Energy & Environment 2023年第1期8卷 4-9页

作者： Jianjun chen Rongqiang Yin Gongda chen Junyu Lang Xiaoping chen Xuefeng chu Junhua Li State Key Joint Laboratory of Environment Simulation and Pollution Control School of EnvironmentTsinghua UniversityBeijingChina School of Physical Science and Technology Shanghai Tech UniversityShanghaiChina Key Laboratory of Architectural Cold Climate Energy Management Ministry of EducationJilin Jianzhu UniversityChangchunChina guangdong Energy Group Science and Technology Research Institute Co. Ltd.GuangdongChina

Thallium(Tl)compounds,highly toxic to biology,are usually released into flue gas during fossil/minerals combustion,and further distributed in water and *** this work,we fundamentally investigated the capture of gaseous Tl_(2)O by industrial V2O5-WO3/TiO_(2)catalyst under working condition in Tl-containing flue *** and theoretical results indicated that the Tl_(2)O has significant electron-feeding capacity and easily donate electron to unoccupied orbitals of TiO_(2),leading to dismutation of Ti 2p and inartificial formation of p-n junction on TiO_(2)surface,which prompted Tl_(2)O selectively interacted with TiO_(2)in flue ***,we proposed and verified an effective way to capture gaseous Tl_(2)O,which offered almost the best choice to eliminate Tl emission from flue gas and expanded the function of the TiO_(2)-based *** formation of p-n junction on commercial V2O5-WO3/TiO_(2)catalyst under working condition was revealed for the first time,which can be a valuable reference for both heterocatalysis and electro/photocatalysis.

关键词： Tl Catalyst SCR Capture p-n junction

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Visualizing Meiotic Chromosome Pairing and Segregation in Interspecific Hybrids of Rice by Genomic in situ Hybridization

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Rice science 2021年第1期28卷 69-80页

作者： Liu Mao-Sen Tseng Shih-Hsuan chen Ting-chu chung Mei-chu Institute of Plant and Microbial Biology Academia SinicaNankangTaipei 11529TaiwanChina

Meiotic disturbances in F1 hybrids and their progenies are still major problems in wide *** investigate the genome affinity reflected in chromosome pairing and segregation,we studied chromosome behaviors during meiosis in two interspecific F1 hybrids[***×***(Om×Oa,BCE genome)and Oa×***(Or,EHJ genome)]by using both traditional staining methods and genomic in situ hybridization(GISH).GISH analysis has been successfully performed on mitotic chromosomes to distinguish different Oryza genomes,but relatively fewer systematic analyses of meiotic chromosomes of interspecific hybrids have been *** the hybrids,highly irregular chromosome behaviors through meiosis resulted in producing microspores with unbalanced *** diakinesis of these two hybrids,most chromosomes present as univalent,with low frequency as bivalents and occasionally as *** a pollen mother cell,2 to 8 bivalents and 0 to 4 trivalents were observed in the hybrid Oa×Or,and 1 to 8 bivalents and 0 to 5 trivalents were observed in the hybrid Om×*** results indicated that 51.52%bivalents in Oa×Or and 79.65%bivalents in Om×Oa involved allosyndetic association,which indicates that recombination and introgression should be possible if viable backcrosses can be recovered even from triploid *** this study,we revealed that the meiotic disturbance due to low affinities between parental genomes is the major reason for the sterility of these two triploid interspecific *** two hybrids showing vigor in reproductive growth are potential genetic resources in future breeding programs.A better understanding of genomic affinities between these distant Oryza species can facilitate planning an effective breeding program by using wide hybridization,and efficient and routine GISH analysis is helpful to monitor alien introgression in the process.

关键词： Oryza wide hybridization genomic affinity allosyndesis autosyndesis pollen viability

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