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Isolation,Purification and Hypoglycemic Activity of Extracellular Polysaccharides from Laetiporus Sulphureus Mycelium

Isolation,Purification and Hypoglycemic Activity of Extracel...

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作者： chibuike augustus chiemeka 沈阳农业大学

学位级别：硕士

The polysaccharides with hyperglycemic activity from Laetiporus sulphureus was extracted and characterized,as well as establishing a nitrogen restriction strategy for increasing polysaccharide *** main research content of this paper is listed below:*** DNA of the strain obtained from Xia matang(Benxi,Liaoning province)was separated by CTAB *** PCR and sequence detection,a 500-750 bp sequence was obtained,and the length of nucleic acid sequence was consistent with the characteristics of fungal gene *** SEM obsered that the mycelium of this strain appeared a lock-form structure with spherical *** sequence alignment and morphological observation,the strain was identified Laetiporus *** six kinds of nitrogen source fermentation test,inorganic nitrogen(potassium nitrate)and organic nitrogen(peptone)were selected as nitrogen sources for restricted nitrogen *** results showed that the optimal nitrogen restriction condition for extracellular polysaccharide of *** mycelium production was 5 mmol/L of potassium nitrate and4 days of *** these conditions,the yield of crude polysaccharide(named e LSP)could reach 14.655 mg/m *** e LSP was isolated by cellulose ion-exchange chromatography and obtained three fractions,which were named as e LSP-I,e LSP-II and e LSP-III,*** antioxidant capacity of the three polysaccharide fractions was detected and the result showed that e LSP-II had the highest DPPH scavenging ***,the e LSP-II was used for further purifing with Sephadex G-100,and a single fraction was collected,named as e *** properties of e LSP-IIa were characterized by HPGPC,FT-IR and *** monosaccharide composition analysis revealed that e LSP-IIa was composed of Glc,Gal,Ara and Gal A with the ratio of 91.0,6.9,1.2 and 0.9,*** molecular weight detecting by HPGPC exhibited that e LSP-IIa was about 8.8×10～*** of the e LSP-IIa revealed a rod-

关键词： Laetiporus sulphureus Nitrogen limitation Antioxidant Separation and purification antidiabetics

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