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Identification of salinity-related genes in ENO2 mutant (eno2~–) of Arabidopsis thaliana

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Journal of Integrative Agriculture 2018年第1期17卷 94-110页

作者： ZHANG Yong-hua CHEN Chao SHI Zi-han cheng hui-mei BING Jie MA Xiao-feng ZHENGChao-xing LI Hong-jie ZHANG Gen-fa Beijing Key Laboratory of Gene Resource and Molecular Development/College of Life Sciences Beijing Normal University Beijing 100875 P.R.China Beijing Normal University Zhuhai 519087 P.R.China National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 P.R. China

Abiotic stress poses a great threat to plant growth and can lead to huge losses in yield. Gene enolase2 （EN02） is important in resistance to abiotic stress in various organisms. ENO2 T-DNA insertion mutant （enoZ） plants of Arabidopsis thaliana showed complete susceptibility to sodium chloride treatment when were analyzed either as whole plants or by measuring root growth during NaCl treatment. Quantitative real-time RT-PCR （RT-qPCR） was performed to investigate the expression profile of EN02 in response to NaCl stress in Arabidopsis. The transcript level of EN02 was rapidly elevated in 300 mmol L-1 NaCl treatment. ENO2 also responded to 300 mmol L 1 NaCl treatment at the protein level. To illuminate the mechanism underlying EN02 resistance to salt at the transcriptional level, we studied the wild-type and enoZ Arabidopsis lines that were treated with 300 mmol L 1 NaCl for 18 h using 454 GS FLX, which resulted in an expressed sequence tag （EST） dataset. A total of 961 up-regulated and 746 down-regulated differentially expressed genes （DEGs） were identified in the pairwise comparison w-r-18 h：eno2^-18 h. The DEGs were identified and functionally annotated using the databases of Gene Ontology （GO） and the Kyoto encyclopedia of genes and genomes （KEGG）. The identified unigenes were subjected to GO analysis to determine biological, molecular, and cellular functions. The biological process was enriched in a total of 20 GO terms, the cellular component was enriched in 13 GO terms, and the molecular function was enriched in 11 GO terms. Using KEGG mapping, DEGs with pathway annotations contributed to 115 pathways. The top 3 pathways based on a statistical analysis were biosynthesis of the secondary metabolites （KO01110）, plant-pathogen interactions （KO04626）, and plant hormone signal transduction （KO04075）. Based on these results, EN02 contributes to increased resistance to abiotic stress. In particular, EN02 is involved in some of the metabolic stress response pathways in

关键词： EN02 NaCI tolerance abiotic stress 454 GS FLX sequencing GO KEGG

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民国时期广西霍乱与近代防疫卫生体系

医学与哲学 2023年第24期44卷 65-70页

作者：程美慧梧州学院马克思主义学院广西梧州543000

民国时期霍乱在广西社会流行,呈现出前期低、中后期高、夏季高发、以区域经济中心向周边扩散的特点。政府组织了隔离消毒、报告疫情、检疫、疫苗接种、防疫宣传和清洁卫生、生产管理药品、出台法律法规、考核医务工作者等近代防疫手段,... 详细信息

民国时期霍乱在广西社会流行,呈现出前期低、中后期高、夏季高发、以区域经济中心向周边扩散的特点。政府组织了隔离消毒、报告疫情、检疫、疫苗接种、防疫宣传和清洁卫生、生产管理药品、出台法律法规、考核医务工作者等近代防疫手段,这在一定程度上保障了民生,遏制了疫情。然而,民众不配合防疫,政府处罚以示惩儆效果欠佳,也未形成有力的疫苗防疫屏障,环境卫生发展不平衡、甚至出现倒退现象,这表明政府应对霍乱存在局限性。在这个过程中,建立并逐渐完善了近代广西防疫卫生体系。

关键词：广西霍乱防疫卫生体系权力

Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future

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Neural Regeneration Research 2025年第4期20卷 946-959页

作者： Zhiyuan Yin Jiahui Kang Xuan cheng hui Gao Shujia Huo Haiwei Xu Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Southwest Eye HospitalSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased *** lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored ***,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various *** in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in ***,there remain issues that hinder improving reprogramming efficiency and ***,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming *** review describes recent progress in relatively successful Müller glia reprogramming *** also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关键词： cell fusion chemical small-molecules epigenetic extracellular matrix immune metabolic mice Müller glia neurodegenerative diseases reprogramming retina regeneration

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如何在体育教学中培养学生的健康人格

沙洋师范高等专科学校学报 2012年第3期13卷 43-45页

作者：程慧美河南中医学院体育部河南郑州450008

为了顺应社会对人才需求的变化,课程改革势在必行。新课程标准指导下的中国教育把呵护学生心理健康与培养学生健全人格提到了一个新的高度。这种情势下,体育教学作为学校教育的重要组成部分,必须对培养学生健康人格做出其应有的贡献。... 详细信息

为了顺应社会对人才需求的变化,课程改革势在必行。新课程标准指导下的中国教育把呵护学生心理健康与培养学生健全人格提到了一个新的高度。这种情势下,体育教学作为学校教育的重要组成部分,必须对培养学生健康人格做出其应有的贡献。文章在分析现行体育教育中存在的问题基础上,就如何在体育教学中培养学生的健康人格提出了相关建议。

关键词：体育教学健康人格问题方法

强化护士伦理教育对急诊科护理投诉的改善分析

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智慧健康 2020年第15期6卷 15-16,52页

作者：成惠梅连州市连州镇中心卫生院广东清远513400

目的探析强化护士伦理教育对急诊科护理投诉的改善效果。方法以2018年4月至2019年9月在我院急诊科接受治疗的100例患者作为主要对象,采用数字随机表达法分组,每组50例。对照组实施急诊科常规护理,观察组护理中对护士伦理教育进行强化再... 详细信息

目的探析强化护士伦理教育对急诊科护理投诉的改善效果。方法以2018年4月至2019年9月在我院急诊科接受治疗的100例患者作为主要对象,采用数字随机表达法分组,每组50例。对照组实施急诊科常规护理,观察组护理中对护士伦理教育进行强化再开展护理工作,比较两组的护理质量、患者满意度和投诉发生率。结果经比较两组的护理质量,观察组的操作技术、态度行为、沟通交流评分均明显高于对照组,两组比较有显著差异(P<0.05);观察组患者对护理人员和护理工作的满意度评分明显高于对照组,两组比较差异显著(P<0.05);观察组的投诉发生率明显低于对照组,差异有统计学意义。结论在急诊科护理工作中,对护士进行伦理教育可提高急诊科的护理质量,同时可提高患者的满意度,投诉率更低,可进一步推广和应用。

关键词：护士伦理教育急诊科护理投诉率

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合金元素对油船用低合金钢腐蚀行为的影响

钢铁研究学报 2017年第6期29卷 506-512页

作者：李玉谦杜琦铭成慧梅河钢集团邯钢公司技术中心河北邯郸056015

采用恒温浸泡的标准试验方法,考察了4种不同合金体系船体钢在强酸性氯离子介质中的腐蚀行为,探讨了合金元素对钢腐蚀行为的影响,结果表明:Cu和Ni对钢的耐蚀性有显著影响,随着Cu、Ni含量的增加,钢的耐蚀性逐渐提高,自腐蚀电位提高,电流... 详细信息

采用恒温浸泡的标准试验方法,考察了4种不同合金体系船体钢在强酸性氯离子介质中的腐蚀行为,探讨了合金元素对钢腐蚀行为的影响,结果表明:Cu和Ni对钢的耐蚀性有显著影响,随着Cu、Ni含量的增加,钢的耐蚀性逐渐提高,自腐蚀电位提高,电流密度显著降低,界面电荷传递电阻明显增大。Cu是提高钢在强酸性氯离子环境中耐蚀性的主要元素,其主要机理为其以再沉积颗粒(100~500nm)的方式在钢的表面富集,并具有较高的稳定性,该沉积颗粒钝化了钢基体,降低了钢的溶解速度。Ni元素在钢中的存在显著提高了钢的基体电位,降低了材料的腐蚀敏感性,但Ni元素在锈层中的富集特征不明显。

关键词：酸性氯离子货油舱腐蚀

Ideal Bi-Based Hybrid Anode Material for Ultrafast Charging of Sodium-Ion Batteries at Extremely Low Temperatures

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NANO-MICRO LETTERS 2025年第1期17卷 1-16页

作者： Bai, Jie Jia, Jian hui Wang, Yu Yang, Chun cheng Jiang, Qing Jilin Univ Key Lab Automobile Mat Sch Mat Sci & Engn Minist Educ Changchun 130022 Peoples R China

Metallic nanoparticles with excellent size controllability and high loading rate are obtained via ultrafast high temperature shock *** Bi/CNRs-15 electrode exhibits an unprecedented rate performance (237.9 mAh g-1 at 2 A g-1) at - 60 degrees C, while the energy density of the full cell can reach to 181.9 Wh kg-1 at - 40 degrees C.A novel strategy of high-rate activation is proposed to obtain high capacity and superior stability at low temperature by creating new active sites for interfacial reaction. Sodium-ion batteries have emerged as competitive substitutes for low-temperature applications due to severe capacity loss and safety concerns of lithium-ion batteries at - 20 degrees C or lower. However, the key capability of ultrafast charging at ultralow temperature for SIBs is rarely reported. Herein, a hybrid of Bi nanoparticles embedded in carbon nanorods is demonstrated as an ideal material to address this issue, which is synthesized via a high temperature shock method. Such a hybrid shows an unprecedented rate performance (237.9 mAh g-1 at 2 A g-1) at - 60 degrees C, outperforming all reported SIB anode materials. Coupled with a Na3V2(PO4)3 cathode, the energy density of the full cell can reach to 181.9 Wh kg-1 at - 40 degrees C. Based on this work, a novel strategy of high-rate activation is proposed to enhance performances of Bi-based materials in cryogenic conditions by creating new active sites for interfacial reaction under large current.

关键词： Bi nanoparticles High temperature shock High-rate activation Ultrafast charging Low-temperature sodium-ion batteries