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Screening and degradation characteristics of a tylosin-degrading strain

Journal of Integrative Agriculture 2020年第4期19卷 1127-1136页

作者： FENG Chang-qing cheng deng-miao FENG Yao QI Wei-ning JIA Zhen-hu Louise WEAVER LIU Yuan-wang LI Zhao-jun College of Life Science Shanxi Normal UniversityLinfen 041004P.R.China Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Environmental and Science Research Ltd. Christchurch Science CentreChristchurch 8041New Zealand

Antibiotics residues have been accumulating in the environment day by day due to overuse of *** antibiotic residues,such as tylosin(TYL),can cause serious environmental problems,which makes it important to eliminate TYL from the *** is important to eliminate TYL from the *** this study,a strain was isolated and purified from fermentation by-product that came from a TYL production *** TYL degrading strain was identified by its morphology,physiological and biochemical reactions and sequencing the PCR-amplified fragments of its 16 S r DNAcoding *** temperature,shaking speed,initial TYL concentration,p H and inoculum sizes were investigated under simulated conditions by using single factor *** results showed that TYL2,a high efficient strain was isolated and was identified as Brevibacillus *** degradation rate of TYL by this strain could reach to 75%with an initial concentration of 25 mg L^-1 within 7 days under conditions of 7%***(v/v,2×108 CFU m L^-1)at p H 7.0 and at 35°*** is interesting that this strain has a very strong ability to degrade the TYL in natural sewage with the degradation rate of 65%within 7 *** result could be helpful for the degradation of TYL and provide guidance for the degradation of other antibiotics.

关键词： antibiotics tylosin screening degrading strain B.borstelensis degradation characteristic

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Microbial community dynamics during composting of animal manures contaminated with arsenic,copper,and oxytetracycline

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Journal of Integrative Agriculture 2021年第6期20卷 1649-1659页

作者： Ebrahim SHEHATA cheng deng-miao MA Qian-qian LI Yan-li LIU Yuan-wang FENG Yao JI Zhen-yu LI Zhao-jun Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Department of Natural Resources and Agricultural Engineering College of AgricultureDamanhour UniversityDamanhour 22511Egypt Research Center for Eco-Environmental Engineering Dongguan University of TechnologyDongguan 523808P.R.China

Effects of the heavy metal copper(Cu), the metalloid arsenic(As), and the antibiotic oxytetracycline(OTC) on bacterial community structure and diversity during cow and pig manure composting were investigated. Eight treatments were applied, four to each manure type, namely cow manure with:(1) no additives(control),(2) addition of heavy metal and metalloid,(3) addition of OTC and(4) addition of OTC with heavy metal and metalloid;and pig manure with:(5) no additives(control),(6) addition of heavy metal and metalloid,(7) addition of OTC and(8) addition of OTC with heavy metal and metalloid. After 35 days of composting, according to the alpha diversity indices, the combination treatment(OTC with heavy metal and metalloid) in pig manure was less harmful to microbial diversity than the control or heavy metal and metalloid treatments. In cow manure, the treatment with heavy metal and metalloid was the most harmful to the microbial community, followed by the combination and OTC treatments. The OTC and combination treatments had negative effects on the relative abundance of microbes in cow manure composts. The dominant phyla in both manure composts included Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. The microbial diversity relative abundance transformation was dependent on the composting time. Redundancy analysis(RDA) revealed that environmental parameters had the most influence on the bacterial communities. In conclusion, the composting process is the most sustainable technology for reducing heavy metal and metalloid impacts and antibiotic contamination in cow and pig manure. The physicochemical property variations in the manures had a significant effect on the microbial community during the composting process. This study provides an improved understanding of bacterial community composition and its changes during the composting process.

关键词： composting heavy metal and metalloid oxytetracycline microbial community principle component analysis redundancy analysis

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The impacts of oxytetracycline on humification during manure composting can be alleviated by adjusting initial moisture contents as illustrated by NMR

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Journal of Integrative Agriculture 2021年第8期20卷 2277-2288页

作者： FENG Yao WANG Gui-zhen LIU Yuan-wang cheng deng-miao FAN Shuang-hu ZHAO Quansheng Jianming XUE ZHANG Shu-qing LI Zhao-jun Key Laboratory of Plant Nutrition and Fertilizer Ministry of Agriculture and Rural Affairs/China-New Zealand Joint Laboratory for Soil Molecular EcologyInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China College of Life Science Langfang Normal UniversityLangfang 065000P.R.China College of Biology and the Environment Nanjing Forestry UniversityNanjing 210037P.R.China Scion Christchurch 8440New Zealand

Oxytetracycline(OTC)residues have been found in soil and water,and they may pose potential risks to agricultural ecological *** of the most impactful ways for OTC to enter the soil and water environments is through excrement used as organic ***,it is important to remove OTC during manure composting and to understand the transformation of the organic materials during composting in the presence of *** the present paper,chicken manure and wheat sawdust spiked with OTC were composted under different initial moisture contents(MC)to evaluate the degradation of OTC and changes of organic matter during the composting *** MC has a significant effect on OTC degradation during composting.A higher MC of 65% was more conducive to OTC degradation(77.4%)and compost maturity compared to the lower ***,the higher MC of 65%could increase the ammonia volatilization by promoting nitrification compared to the lower *** increase in the initial MC could improve the composting *** results illustrated that the presence of OTC could affect the internal transformation of aliphatics,and OTC inhibited compost ***,an initial MC of 55–65% can alleviate the impacts of OTC on humification during manure composting.

关键词： oxytetracycline degradation humification moisture content composting physico-chemical property

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Transition metals for stabilizing lithium metal anode:advances and perspectives

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Tungsten 2024年第1期6卷 212-229页

作者： Si-cheng miao Ye Jia Zhi-Wen deng Yan deng Ruo-Xuan Chen Xue-Mei Zhang Chang-Haoyue Xu Meng Yao Wen-Long Cai Department of Advanced Energy Materials College of Materials Science and EngineeringSichuan UniversityChengdu 610064China

As the capacity of lithium-ion batteries gradually reaches its limit,the high-capacity characteristics of lithium metal batteries(LMBs)make them one of the most promising electrochemical energy storage devices ***,uncontrolled lithium dendrite growth can cause poor cell performance and severe safety issues,seriously slowing down the commercialization of *** metals(TMs)are lithiophilic materials that effectively stabilize lithium metal anode(LMA)and inhibit dendrite ***,the growth of lithium dendrites and the performance of TMs in stabilizing lithium metals are comprehensively discussed in this ***,we elucidated the formation mechanisms of lithium dendrite,and we put forward several strategies to suppress lithium dendrite formation based on the understanding of these ***,the application of TMs and their compounds to stabilize LMA and their corresponding mechanisms are comprehensively ***,further research perspectives of TMs in stabilizing LMA are emphasized in detail.

关键词： Transition metals Lithium metal batteries Lithium dendrite Solid electrolyte interphase Formation mechanism

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Continuous-flow synthesis of polysubstituted γ-butyrolactones via enzymatic cascade catalysis

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Chinese Chemical Letters 2024年第4期35卷 404-408页

作者： Liliang Chu Xiaoyan Zhang Jianing Li Xuelei deng miao Wu Ya cheng Weiping Zhu Xuhong Qian Yunpeng Bai State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for BiomanufacturingEast China University of Science and TechnologyShanghai 200237China The Extreme Optoelectromechanics Laboratory School of Physics and Materials ScienceEast China Normal UniversityShanghai 200241China

Polysubstituted chiral γ-butyrolactones are the core structural units of many natural products and high value-added flavors and fragrances used in the food and cosmetic industry. Current enzymatic cascade synthesis of these molecules faces the problems of low enzyme activity and phase separation in batch reaction, resulting in low productivity. Herein, we report a new continuous-flow process to synthesize the optically pure Nicotiana tabacum lactone(3S,4S)-4a and whisky lactone(3R,4S)-4b from α,β-unsaturatedγ-ketoesters. A new ene reductase(ER) from Swingsia samuiensi(Ss ER) and a carbonyl reductase(Ss CR)were engineered by directed evolution to improve their activity and thermostability. The continuous-flow preparative reactions were performed in two 3D microfluidic reactors, generating(3S,4S)-4a(99% ee and87% de) and(3R,4S)-4b(99% ee and 98% de) with space-time yields 3 and 7.4 times higher than those of the batch reactions. The significant enhancement in the productivity of enzyme cascade catalysis brought by cutting-edge continuous microfluidic technology will benefit the general multi-enzyme catalytic systems in the future.

关键词： Enzyme catalysis Cascade catalysis Directed evolution Continuous-flow γ-Butyrolactones

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Reconfigurable and polarization-dependent optical filtering for transflective full-color generation utilizing low-loss phase-change materials

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Journal of Semiconductors 2024年第7期45卷 46-53页

作者： Shuo deng Mengxi Cui Jingru Jiang Chuang Wang Zengguang cheng Huajun Sun Ming Xu Hao Tong Qiang He Xiangshui miao School of Integrated Circuits Huazhong University of Science and TechnologyWuhan 430074China Hubei Yangtze Memory Laboratories Wuhan 430205China School of Microelectronics Fudan UniversityShanghai 200433China

All-dielectric metasurface, which features low optical absorptance and high resolution, is becoming a promising candidate for full-color generation. However, the optical response of current metamaterials is fixed and lacks active tuning. In this work, we demonstrate a reconfigurable and polarization-dependent active color generation technique by incorporating low-loss phase change materials(PCMs) and CaF_2 all-dielectric substrate. Based on the strong Mie resonance effect and low optical absorption structure, a transflective, full-color with high color purity and gamut value is achieved. The spectrum can be dynamically manipulated by changing either the polarization of incident light or the PCM state. High transmittance and reflectance can be simultaneously achieved by using low-loss PCMs and substrate. The novel active metasurfaces can bring new inspiration in the areas of optical encryption, anti-counterfeiting, and display technologies.

关键词： structural color reconfigurable all-dielectric metasurfaces phase change material

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A New Vehicle Detection Framework Based on Feature-Guided in the Road Scene

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Computers, Materials & Continua 2024年第1期78卷 533-549页

作者： Tianmin deng Xiyue Zhang Xinxin cheng School of Traffic and Transportation Chongqing Jiaotong UniversityChongqing400074China

Vehicle detection plays a crucial role in the field of autonomous driving ***,directly applying deep learning-based object detection algorithms to complex road scene images often leads to subpar performance and slow inference speeds in vehicle *** a balance between accuracy and detection speed is crucial for real-time object detection in real-world road *** paper proposes a high-precision and fast vehicle detector called the feature-guided bidirectional pyramid network(FBPN).Firstly,to tackle challenges like vehicle occlusion and significant background interference,the efficient feature filtering module(EFFM)is introduced into the deep network,which amplifies the disparities between the features of the vehicle and the ***,the proposed global attention localization module(GALM)in the model neck effectively perceives the detailed position information of the target,improving both the accuracy and inference speed of ***,the detection accuracy of small-scale vehicles is further enhanced through the utilization of a four-layer feature pyramid *** results show that FBPN achieves an average precision of 60.8% and 97.8% on the BDD100K and KITTI datasets,respectively,with inference speeds reaching 344.83 frames/s and 357.14 frames/*** demonstrates its effectiveness and superiority by striking a balance between detection accuracy and inference speed,outperforming several state-of-the-art methods.

关键词： Driverless car vehicle detection channel attention mechanism deep learning

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Continuous-flow enzymatic synthesis of chiral lactones in a three-dimensional microfluidic reactor

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Chinese Chemical Letters 2024年第3期35卷 299-303页

作者： Xuelei deng Meng Fan miao Wu Xiaoyan Zhang Ya cheng Jianye Xia Yingping Zhuang Weiping Zhu Xuhong Qian Yunpeng Bai State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for BiomanufacturingEast China University of Science and TechnologyShanghai 200237China The Extreme Optoelectromechanics Laboratory School of Physics and Materials ScienceEast China Normal UniversityShanghai 200241China Tianjin Institute of Industrial Biotechnology Chinese Academy of SciencesTianjin 300308China

A new continuous-flow process for the enzymatic synthesis of optically pureγ-lactones,which are used as flavors and fragrances in the food and cosmetic industries,was developed in a three-dimensional microfluidic *** microchannels(175 mm in length,0.9 mm in depth,and 1.72 mL in volume)were carved precisely inside a single borosilicate glass(90 mm×75 mm×12 mm)with ultrafast femtosecond laser *** flow field analysis and reaction simulation showed that the mixing of substrates and enzymes was enhanced,allowing the adjustment of residence time in a wide ***_(V4),a carbonyl reductase with excellent catalytic activity and enantioselectivity towardγ/δ-keto acids,was employed for the asymmetric synthesis of various chiral lactones.30 mmol/L(R)-γ-decalactone(3g)can be obtained in 26 s with a space-time yield(STY)up to 16,877 g L^(-1)d^(-1),which is 14.4 times higher than the highest STY of batch reaction reported *** continuous-flow process was applied to the synthesis of 6 chiral *** addition,the scaled-up synthesis of 3g was carried out in 6 cascade microreactors continuously for 6 h,demonstrating the feasibility and stability of the 3D continuous-flow process in enzymatic synthesis of optically pure compounds.

关键词： Continuous-flow Flow chemistry Microreactor Biocatalysis Carbonyl reductase Asymmetric reduction

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In situ sulfur-doped mesoporous tungsten oxides for gas sensing toward benzene series

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Chinese Chemical Letters 2024年第7期35卷 502-507页

作者： Yu deng Yan Liu Yonghui deng Jinsheng cheng Yidong Zou Wei Luo State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China Department of Chemistry State Key Laboratory of Molecular Engineering of PolymersShanghai Key Laboratory of Molecular Catalysis and Innovative MaterialsiChEMFudan UniversityShanghai 200433China Department of Polymeric Materials School of Materials Science and EngineeringTongji UniversityShanghai 201804China Henry-Fork School of Food Sciences Shaoguan UniversityShaoguan 512005China State Key Lab of Transducer Technology Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China

Benzene series as highly toxic gases have inevitably entered human life and produce great threat to human health and ecological environment,and thus it is distinctly meaningful to monitor benzene series with quickly,real-time and efficient ***,novel sulfur-doped mesoporous WO_(3)materials were synthesized via classical in-situ solvent evaporation induced co-assembly strategy combined with doping engineering,which possessed highly crystallized frameworks,high specific surface area(40.9–63.8 m^(2)/g)and uniform pore size(~18 nm).Benefitting from abundant oxygen vacancy and defects via S-doping,the tailored mesoporous S/m WO_(3)exhibited excellent benzene sensing performance,including high sensitivity(50 ppm vs.48),low detection limit(ca.500 ppb),outstanding selectivity and favorable *** addition,the reduction of band gap resulted from S-doping promotes the carrier migration in the sensing materials and the reaction at the gas–solid sensing *** provides brand-new approach to design sensitive materials with multiple reaction sites.

关键词： Mesoporous materials Benzene series Tungsten oxides Sulfur doping Gas sensor

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Progress in the application of polymer fibers in solid electrolytes for lithium metal batteries

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Journal of Energy Chemistry 2024年第5期92卷 26-42页

作者： Junbao Kang Nanping deng Bowen cheng Weimin Kang State Key Laboratory of Separation Membranes and Membrane Processes/National Center for International Joint Research on Separation Membranes School of Textile Science and EngineeringTiangong UniversityTianjin 300387China

Solid state lithium metal batteries(SSLMBs)are considered to be one of the most promising battery systems for achieving high energy density and excellent safety for energy storage in the ***,current existed solid-state electrolytes(SSEs)are still difficult to meet the practical application requirements of *** this review,based on the analysis of main problems and challenges faced by the development of SSEs,the ingenious application and latest progresses including specific suggestions of various polymer fibers and their membrane products in solving these issues are emphatically ***,the inherent defects of inorganic and organic electrolytes are pointed ***,the application strategies of polymer fibers/fiber membranes in strengthening strength,reducing thickness,enhancing thermal stability,increasing the film formability,improving ion conductivity and optimizing interface stability are discussed in detail from two aspects of improving physical structure properties and electrochemical ***,the researches and development trends of the intelligent applications of high-performance polymer fibers in SSEs is *** review intends to provide timely and important guidance for the design and development of polymer fiber composite SSEs for SSLMBs.

关键词： Composite solide lectrolytes Polymer fibers Solid-state lithium metal batteries Solid-stateel ectrolytes Nanofiber membranes

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