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Polysaccharide extracts of Astragalus membranaceus and Atractylodes macrocephala promote intestinal epithelial cell migration by activating the polyamine-mediated K^+ channel

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Chinese Journal of Natural Medicines 2018年第9期16卷 674-682页

作者： ZENG Dan HU Can LI Ru-Liu LIN chuan-Quan CAI Jia-Zhong WU Ting-Ting SUI jing-jing lu Wen-Biao chen Wei-Wen Pi-wei Institute Guangzhou University of Chinese Medicine College of Traditional Chinese Medicine Guangzhou University of Chinese Medicine

Astragalus membranaceus(Radix Astragali, RA) and Atractylodes macrocephala(Rhizoma Atractylodis Macrocephalae, RAM) are often used to treat gastrointestinal diseases. In the present study, we determined the effects of polysaccharides extracts from these two herbs on IEC-6 cell migration and explored the potential underlying mechanisms. A migration model with IEC-6 cells was induced using a single-edged razor blade along the diameter of cell layers in six-well polystyrene plates. The cells were grown in control--media or media containing spermidine(5 μmol·L^(-1), SPD), alpha-difluoromethylornithine(2.5 mmol·L^(-1), DFMO), 4-Aminopyridine-(40 μ-mol·L^(-1), 4-AP), the polysaccharide extracts of RA or RAM(50, 100, or 200 mg·L^(-1)), DFMO plus SPD, or DFMO plus polysaccharide extracts of RA or RAM for 12 or 24 h. Next, cytosolic free Ca^(2+)([Ca^(2+)]cyt) was measured using laser confocal microscopy, and cellular polyamine content was quantified with HPLC. Kv1.1 mRNA expression was assessed using RT-qPCR and Kv1.1 and RhoA protein expressions were measured with Western blotting analysis. A cell migration assay was carried out using Image-Pro Plus software. In addition, GC-MS was introduced to analyze the monosaccharide composition of both polysaccharide extracts. The resutls showed that treatment with polysaccharide extracts of RA or RAM significantly increased cellular polyamine content, elevated [Ca^(2+)]cyt and accelerated migration of IEC-6 cells, compared with the controls(P lular polyamine content and [Ca^(2+)]cyt, but also restored IEC-6 cell migration to control level(P lucose, and D-galactose was 1.0:14.1:0.3:19.9:181.3

关键词： Intestinal epithelial cell (IEC-6) Cell migration Polysaccharide extracts Astragalus membranaceus Atractylodes macrocephala Polyamines K^+ channel

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儿科重症监护病房患儿肠内营养中断的研究

护理学杂志 2024年第16期39卷 94-98页

作者：杨玉霞陆国平顾莺胡静陈伟明沈伟杰高萱朱婷复旦大学附属儿科医院重症医学科上海201102

目的调查儿科重症监护病房患儿肠内营养中断现状,分析相关影响因素,为患儿肠内营养中断干预措施制定提供参考。方法选取儿童专科医院重症监护病房299例患儿为研究对象,调查患儿肠内营养中断情况及原因。将患儿分为肠内营养中断组与肠内... 详细信息

目的调查儿科重症监护病房患儿肠内营养中断现状,分析相关影响因素,为患儿肠内营养中断干预措施制定提供参考。方法选取儿童专科医院重症监护病房299例患儿为研究对象,调查患儿肠内营养中断情况及原因。将患儿分为肠内营养中断组与肠内营养未中断组,分析肠内营养中断对患儿临床结局的影响。结果299例患儿发生肠内营养中断190例(63.5%),共373例次,平均每例患儿发生营养中断2.0(1.0,3.0)次,发生营养中断16.0(8.0,29.3)h。喂养不耐受是营养中断最常见的原因;肠内营养中断组患儿机械通气时间、多脏器功能不全、PICU住院时间及PICU住院费用显著高于未中断组(均P<0.05)。结论儿科重症监护病房患儿肠内营养中断发生率高,影响患儿结局。应制定肠内营养中断的干预策略,缩短肠内营养中断时间,减少不必要的肠内营养中断。

关键词：儿童重症监护病房肠内营养营养中断影响因素喂养不耐受机械通气营养管理

Plant Temperature and Its Simulation Model of Thermo-Sensitive Genic Male Sterile Rice

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Rice science 2008年第3期15卷 223-231页

作者： lu chuan-gen XIA Shi-jian chen jing Hu Ning YAO Ke-min Institute of Food Crops Jiangsu Academy of Agricultural Sciences Nanjing 210014 China Institute of Applied Meteorology Nanjing University of lnformation Science and Technology Nanjing 210044 China

Plant temperature （Tp） and its relations to the microclimate of rice colony and irrigation water were studied using a thermo-sensitive genic male sterile （TGMS） rice line, Pei＇ai 64S. Significant differences in the daily change of temperature were detected between Tp and air temperature at the height of 150 cm （TA）. From 8：00 to 20：00, Tp was lower than TA, but they were similar during 21：00 to next 7：00. The maximum Tp occurred one hour earlier than the maximum TA, though they both reached the minimum at 6：00. Tp fluctuated less than TA. At the same height, during 6：00-13：00, Tp was higher than air temperature （Ta）, and Tp reached the maximum one hour earlier than Ta. During the rest time on sunny day, Tp was close to or even a little lower than Ta. On overcast day, Tp was higher than Ta in the whole day, and both maximized at the same time. In addition, Tp was regulated by solar radiation, cloudage and wind speed in daytime, and by irrigation water at night. The present study indicated that a TA of 29.6℃ was the critical point, at which Tp was increased or decreased by irrigation water. Tp and the difference between water and air temperatures showed a conic relation. Tp fluctuation was also regulated by the absorption or reflection of solar radiation by leaves during daytime and release of heat energy during nighttime. By analysis on correlation and regression simulation, two models of Tp were established.

关键词： rice plant temperature air temperature simulation model thermo-sensitive genic male sterility

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钱宝钧高等教育思想与现代传承研究

高分子通报 2024年第9期37卷 1165-1171页

作者：陈丽芸周莹杰陆晓芳马敬红李莉莉丁明利李斌荣东华大学材料科学与工程学院纤维材料改性国家重点实验室东华大学研究生院上海201620

以唯物史观为指导,在访谈及相关文献资料检索基础上,系统梳理了钱宝钧先生高等教育实践经历,着重论述了他坚持思想引领、坚持产教融合和坚持德智体美劳(五育)并举的教育思想,总结了他严谨治学、注重创新、推动科技进步和矢志不移攻坚克... 详细信息

以唯物史观为指导,在访谈及相关文献资料检索基础上,系统梳理了钱宝钧先生高等教育实践经历,着重论述了他坚持思想引领、坚持产教融合和坚持德智体美劳(五育)并举的教育思想,总结了他严谨治学、注重创新、推动科技进步和矢志不移攻坚克难的科学研究成绩,发掘了他注重交叉和团队建设、注重国内外学术交流与合作以及积极拓展平台延揽人才的师资队伍建设理念,并从四个方面总结了他的高等教育思想和现代传承的路径。

关键词：教育思想科学研究人才队伍建设钱宝钧

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Microwave triggered multifunctional nanoplatform for targeted photothermal-chemotherapy in castration-resistant prostate cancer

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Nano Research 2023年第7期16卷 9688-9700页

作者： Pengyuan Liu Yajun Wu Xiaogang Xu Xudong Fan chuan Sun Xiaojie chen Jindan Xia Shiting Bai Li Qu Huasong lu jing Wu Jun chen Ji-Gang Piao Zhibing Wu Department of Oncology Affiliated Zhejiang HospitalZhejiang University School of MedicineHangzhou 310013China School of Pharmaceutical Sciences Zhejiang Chinese Medical UniversityHangzhou 310053China Department of Radiation Oncology Affiliated Zhejiang HospitalZhejiang University School of MedicineHangzhou 310013China Geriatrics Institute of Zhejiang Province Department of GeriatricsAffiliated Zhejiang HospitalZhejiang University School of MedicineHangzhou 310030China Zhejiang Provincial Key Laboratory for Cancer Molecular Cell Biology Life Sciences InstituteZhejiang UniversityHangzhou 310058China TCM Dispensary Affiliated Zhejiang HospitalZhejiang University School of MedicineHangzhou 310013China Department of Breast Surgery Affiliated Hangzhou First People’s HospitalZhejiang University School of MedicineHangzhou 310006China

Lacking a precise targeting strategy,castration-resistant prostate cancer(CRPC)is still hard to be treat *** treatment options that can accurately target CPRC is an important issue with urgent *** this study,a novel nanotechnologybased strategy had been developed for the precise target treatment of *** combining microwaves and photothermal therapy(PTT),this nanoplatform,cmHSP70-PL-AuNC-DOX,targets tumor tissues with outstanding precision and achieves better anti-tumor activity by simultaneously eliciting photothermal and chemotherapeutic *** nanotechnology,cmHSP70-modified and thermo-sensitive liposome-coated AuNC-DOX were prepared and used for CRPC-targeted photothermal ablation and ***(DOX)was selected as the chemotherapeutic agent for *** terms of the curative scheme,prostate tissues were firstly pre-treated with microwaves to induce the expression of heat shock protein 70(HSP70)and its migration to the cell membrane,which was then targeted by HSP70 antibody(cmHSP70)coated on the nanoparticles to achieve accurate drug *** nanoplatform then achieved precise ablation and controlled release of DOX under external near-infrared(NIR)*** the implementation,the targeting,cell killing,and safety of this therapeutical strategy had been verified in vivo and in *** work establishes an accurate,controllable,efficient,non-invasive,and safe treatment platform for targeting CRPC,provides a rational design for CRPC’s PTT,and offers new prospects for nanomedicines with great precision.

关键词： castration-resistant prostate cancer gold nanocage heat shock protein 70 targeted drug delivery photothermalchemotherapy

Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells

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World Journal of Gastroenterology 2012年第48期18卷 7158-7165页

作者： ying-Hua chen Tian Lan jing Li Chun-Hui Qiu Teng Wu Hong-Ju Gou Min-Qiang lu The First Affiliated Hospital of Sun Yat-sen University Institute of Vascular Biology Guangdong Pharmaceutical University Guangzhou PTDS Biotechnology Science and Technology Co.Ltd. Department of Hepatology The Third Affiliated Hospital of Sun Yat-sen University

AIM:To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver ***:Male Sprague-Dawley rats underwent common bile duct ligation(BDL) for 14 d and were treated with Gardenia jasminoides by *** ef-fects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells(LX-2) in ***:Treatment with Gardenia jasminoides decreased serum alanine aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,146.6 ± 15 U/L vs 77 ± 6.5 U/L,P = 0.0007) and aspartate aminotransferase(BDL vs BDL + 100 mg/kg Gardenia jasminoides,188 ± 35.2 U/L vs 128 ± 19 U/L,P = 0.005) as well as hydroxyproline(BDL vs BDL + 100 mg/kg Gardenia jasminoides,438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue,P = 0.004) after ***,Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1(TGF-β1),collagen type?Ⅰ?(Col?Ⅰ) and α-smooth muscle actin(α-SMA).Gardenia jasminoides significantly suppressed the upregulation of TGF-β1,Col?Ⅰand α-SMA in LX-2 exposed to recombinant TGF-β***,Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 ***:Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.

关键词： Gardenia jasminoides Liver fibrosis Collagen typeⅠ Transforming growth factor-β1/Smad2 pathway α-smooth muscle actin

Analysis and Simulation of Plant Type on Canopy Structure and Radiation Transmission in Rice

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Rice science 2013年第3期20卷 229-237页

作者： HU Ning lu chuan-gen YAO Ke-min chen jing ZHANG Xiao-cui College of Applied Meteorological Science Nanjing University of Information Science and Technology Institute of Food Crops Jiangsu Academy of Agricultural Sciences Agricultural College Yangzhou University

Three typical hybrid rice cultivars, together with three artificially modified plant types by the application of N fertilizer during the elongation of the two uppermost leaves were used to analyze how the plant type affected the layered leaf area and radiation transmission. Plant type factors, layered leaf area and radiation distribution were measured at the full heading, 10 d and 25 d after full heading stages, respectively. A model for calculating the layered leaf area from plant type factors was established and validated to determine the effects of plant type factors on the layered leaf area for the three hybrids. Furthermore, the relationship between layered leaf area and radiation transmission was established by using the radiation transmission model. The effects of the plant type factors on the radiation transmission for the three hybrids were evaluated by using this model. Finally, a method was established to describe the canopy structure, such as the layered leaf area index and the radiation distribution in the rice canopy.

关键词： rice plant type layered leaf area index radiation simulation moclel

基于网络药理学和分子对接探究白芍-麦冬药对治疗干燥综合征的作用机制

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云南中医中药杂志 2024年第1期45卷 55-62页

作者：陈莹莹万磊刘健黄传兵李舒李方泽胡赛赛程静安徽中医药大学安徽合肥230038 安徽中医药大学第一附属医院安徽合肥230032

目的运用网络药理学和分子对接探究白芍-麦冬药对治疗干燥综合征(sjogren syndrome,SS)的作用机制。方法通过BATMAN、Gene Cards、OMIM、Drug Bank、TTD、DISGENET数据库和Cytoscape软件发现并探究相关治疗靶点作用关系。通过String数... 详细信息

目的运用网络药理学和分子对接探究白芍-麦冬药对治疗干燥综合征(sjogren syndrome,SS)的作用机制。方法通过BATMAN、Gene Cards、OMIM、Drug Bank、TTD、DISGENET数据库和Cytoscape软件发现并探究相关治疗靶点作用关系。通过String数据库构建蛋白质-蛋白质相互作用网络图。通过DAVID平台进行GO与KEGG富集分析。使用AutoDock Tools1.5.7软件进行分子对接。结果白芍-麦冬有57个有效活性成分,362个作用靶点,SS有1219个疾病相关靶点。主要有效成分为豆甾醇、没食子酸、β-谷甾醇,核心靶点AKT1、IL1B、CAT、IGF1。GO分析中,GO生物过程(biological process,BP)主要包括基因表达的负调控、脂多糖的反应、RNA聚合酶II启动子转录的正向调节等;GO细胞组分(cellular components,CC)主要包括细胞表面、细胞外区域、细胞外体等;GO分子功能(molecular function,MF)主要包括血红素结合、类固醇结合、RNA聚合酶II转录因子活性等。KEGG富集分析中主要包括MAPK信号通路、TNF信号通路、cAMP信号传导途径等;分子对接实验表明其主要活性成分与核心靶点之间存在良好的结合活性。结论白芍-麦冬药对通过多靶点、多通路发挥治疗SS的作用。

关键词：网络药理学分子对接白芍-麦冬干燥综合征

电针对慢性疲劳综合征大鼠行为学及海马炎性因子的影响

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针刺研究 2024年第3期49卷 274-282页

作者：屈媛媛冯楚文孙忠人孙维伯鲁菁邵玉莹郭述豪李彬彬陈涛杨添淞黑龙江中医药大学第二临床医学院哈尔滨150040 黑龙江中医药大学第一临床医学院哈尔滨150040 广州医科大学附属中医医院广州510130 哈尔滨医科大学第一临床医学院哈尔滨150076

目的:观察电针对慢性疲劳综合征(CFS)大鼠行为学及海马炎性因子变化的影响,探讨电针治疗CFS的作用机制。方法:SD大鼠随机分为对照组、模型组和电针组,每组9只。采用改良慢性多因素复合应激刺激法复制CFS模型。电针组大鼠电针“神庭”透... 详细信息

目的:观察电针对慢性疲劳综合征(CFS)大鼠行为学及海马炎性因子变化的影响,探讨电针治疗CFS的作用机制。方法:SD大鼠随机分为对照组、模型组和电针组,每组9只。采用改良慢性多因素复合应激刺激法复制CFS模型。电针组大鼠电针“神庭”透刺“百会”和“大椎”,15 min/次,每日2次,治疗14 d。用实验大鼠一般情况半定量评分评估大鼠一般状态,Morris水迷宫实验评价大鼠学习记忆能力,旷场实验评价大鼠焦虑抑郁状态,力竭跑台时间评估大鼠疲劳情况,HE染色法观察大鼠海马CA1区神经元形态结构,ELISA法检测大鼠海马和血清中白细胞介素(IL)-10、IL-17、转化生长因子(TGF)-β1含量,免疫荧光染色法检测海马CA1区IL-10、IL-17、TGF-β1表达。结果:与对照组比较,模型组大鼠一般情况半定量评分升高(P<0.05),逃避潜伏期延长(P<0.05),穿越原平台次数减少(P<0.05),穿格次数及进入中央区次数增加(P<0.05),力竭跑台时间缩短(P<0.05);模型组海马区神经元数量减少,排列紊乱,结构疏松,少量神经元细胞核缺失;模型组海马和血清中的IL-10含量降低(P<0.05),IL-17、TGF-β1含量升高(P<0.05);模型组海马中的IL-10免疫荧光强度降低(P<0.05),IL-17、TGF-β1免疫荧光强度升高(P<0.05)。与模型组比较,电针组大鼠一般情况半定量评分降低(P<0.05),逃避潜伏期缩短(P<0.05),穿越原平台次数增多(P<0.05),穿格次数及进入中央区次数减少(P<0.05),力竭跑台时间延长(P<0.05);电针组海马神经元数量增加,排列整齐,结构较紧密,坏死神经元减少;电针组海马和血清中的IL-10含量升高(P<0.05),IL-17、TGF-β1含量降低(P<0.05);电针组海马IL-10免疫荧光强度升高(P<0.05),IL-17、TGF-β1免疫荧光强度降低(P<0.05)。结论:电针可缓解CFS大鼠疲劳程度,提高空间学习、记忆能力,可能与调节外周及中枢炎性因子有关。

关键词：慢性疲劳综合征电针炎性因子

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