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Isolation and Characterization of Ell Gene Promoter from Tomato

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Agricultural Sciences in China 2006年第9期5卷 661-669页

作者： chen yin-hua OUYANG Bo LI Han-xia YE Zhi-biao National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P.R.China National Center for Vegetable Improvement （Central China） Huazhong Agricultural University Wuhan 430070 P.R. China Key Laborotory for Tropical Biological Resources Ministry of Education Haikou 570228 P.R. China College of Life Science and Agriculture Hainan University Haikou 570228 P.R.China

A fragment of 2000 bp upstream sequence of Ell clone was amplified from genomic DNA of the tomato cultivar Zhongshu- 5. Sequence analysis showed that the upstream contains the regulatory elements： TATA box （-29 - -22）, CAAT box （-193 - -189）, wound, and drought response elements. Expression vectors of Ell promoter gus fusion were constructed with the promoters of 1 200 and 2 000 bp regions, respectively. Transgenic tomato plants were obtained through Agrobacteriummediated transformation. Histochemical analysis of GUS activity in various tissues showed that the two promoters were able to direct fruit-specific gene expression. The expression driven by promoter of 2 000 bp upstream fragment could increase GUS activity with the maturation of tomato fruits. The promoter of -1 200 bp fragment could direct gus gene expression in fruits with the inductions of drought and wounding. The regulatory region for fruit-specificity was probably located in the region of 1 200 bp of 5′-flanking sequence and some positive regulatory elements or enhancers may exist in the region from -1 200 to -2000 bp.

关键词： Ell gene fruit-specific promoter cloning tomato

Full moment tensor inversion constrained by doublecouple focal mechanism for induced seismicity

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Earthquake Science 2020年第4期33卷 177-193页

作者： Yuyang Tan Haijiang Zhang Junlun Li chen yin Furong Wu Key Lab of Submarine Geosciences and Prospecting Techniques MOE College of Marine GeoscienceOcean University of ChinaQingdao 266100China Laboratory of Seismology and Physics of Earth’s Interiors School of Earth and Space SciencesUniversity of Science and Technology of ChinaHefei 230026China School of Environment and ResourceSouthwest University of Science and Technology Mianyang 621010China Bureau of Geophysical Prospecting INC. China National Petroleum CorporationChengdu 610213China

In this study,we propose a new method to determine full moment tensor solution for induced *** method generalizes the full waveform matching algorithm we have developed to determine the double-couple(DC)focal mechanism based on the neighbourhood *** major difference between the new method and the former one is that we adopt a new misfit function to constrain the candidate moment tensor solutions with respect to a reference DC solution in addition to other misfit terms characterizing the waveform *** synthetic tests using a real passive seismic survey geometry,the results show the new constraint can help better recover the DC components of inverted moment *** further investigate how errors in the velocity model and source location affect the moment tensor *** synthetic test results indicate that the constrained inversion is robust in recovering both the DC and non-DC *** also test the proposed method on several real induced events in an oil/gas field in Oman using the same observation system as synthetic *** it is found that the full moment tensor solutions without using the DC constraints have much larger non-DC components than solutions with the DC constraints,both solutions are able to fit the observed waveforms at similar *** synthetic and real test results suggest the proposed DC constrained inversion method can reliably retrieve full moment tensor solutions for the induced seismicity.

关键词： induced seismicity moment tensor double-couple waveform inversion neighbourhood algorithm

Structure Styles of Mesozoic-Cenozoic U-bearing Rock Series in Northern China

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Acta Geologica Sinica(English Edition) 2016年第6期90卷 2104-2116页

作者： JIN Ruoshi MIAO Peisen SIMA Xianzhang LI Jianguo ZHAO Hualei ZHAO Fengqing FEN Xiaoxi chen yin chen Lulu ZHAO Lijun ZHU Qiang Tianjin Center China Geological Survey Tianjin 300170 China Key Laboratory of Uranium Mineral Resources China Geological Survey Tianjin 300170 China

In Northern China, sandstone-type uranium （U） deposits are mostly developed in Mesozoic-Cenozoic basins. These U deposits are usually hosted in unvarying horizons within the basins and exhibit typical U-forming sedimentary associations, which is referred to as U-bearing rock series. This study describes the structural features of U-bearing rock series within the main Mesozoic-Cenozoic U-producing continental basins in Kazakhstan, Uzbekistan, and Russia in the western segment of the Central Asian Metallogenic Belt （CAMB）, and Northern China in the eastern segment of the CAMB. We analyze the basic structural conditions and sedimentary environments of U-bearing rock series in Northern China and classify their structural styles in typical basins into river valley, basin margin, and intrabasin uplift margin types. The intrabasin uplift margin structural style proposed in this study can be used to indicate directions for the exploration of sandstone-type U deposits hosted in the center of a basin. At the same time, the study of structural style provides a new idea for exploring sandstone-type U deposits in Mesozoic-Cenozoic basins and it is of great significance to prospecting of sandstone-type uranium deposits.

关键词： Sandstone-type uranium deposit U-bearing rock series structural style intrabasin uplift margin type Mesozoic-Cenozoic continental basin

Effects of Rolling Parameters on Texture and Formability of High Strength Ultra-Low Carbon BH Steel

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Journal of Iron and Steel Research International 2013年第6期20卷 75-81页

作者： chen yin-li SU Lan ZHAO Ai-min KUAI Zhen LI Ben-hai LIU Guang-ming Engineering Research Institute University of Science and Technology Beijing Shougang Research Institute of Technology

The effects of hot rolling and cold rolling parameters on texture and r （ plastic strain ratio ） value of high strength ultra low carbon bake hardening （ ULC-BH ） steels are studied with orientation distribution function （ ODF ） structural analysis *** hot rolling , the high strength ULC-BH steel sheet has weakγ-fiber with uniform orientation distributions , and weakα-fiber , of which { 445 } component forms a high intensity peak at coiling temperature of 750℃.After cold rolling , both { 111 } -{ 111 } intensity on theγ-fiber and{ 111 -}{ 112 } intensity on theα-fiber *** a result of substitutional solute elements Mn and P being added to the steel , strong { 112 } deformation texture component is observed onα-fiber , especially at 80%cold rolling reduction , and this leads to the strong { 111 } recrystallization texture after *** increase of cold rolling reduction shifts the maximum intensity on theγ-fiber from { 111 } to { 111 } .After annea-ling , a very strongγ-fiber is obtained , with intensity peak at { 111 } component when cold rolling reduction reaches 80%.Increasing coiling temperature and cold rolling reduction improveγ-fiber intensity and r value , resulting in good deep drawability.

关键词： hot rolling cold rolling texture bake hardening steel

Optical cross-talk reduction in a quantumdot-based full-color micro-light-emitting-diode display by a lithographic-fabricated photoresist mold

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Photonics Research 2017年第5期5卷 411-416页

作者： HUANG-YU LIN CHIN-WEI SHER DAN-HUA HSIEH XIN-yin chen HUANG-MING PHILIP chen TENG-MING chen KEI-MAY LAU CHYONG-HUA chen CHIEN-CHUNG LIN HAO-CHUNG KUO Department of Photonics and Institute of Electro-Optical Engineering National Chiao Tung University Department of Applied Chemistry National Chiao Tung University Department of Electric and Computer Engineering Hong Kong University of Science and Technology Institute of Photonic System National Chiao Tung University

In this study, a full-color emission red–green–blue(RGB) quantum-dot(QD)-based micro-light-emitting-diode(micro-LED) array with the reduced optical cross-talk effect by a photoresist mold has been demonstrated. The UV micro-LED array is used as an efficient excitation source for the QDs. The aerosol jet technique provides a narrow linewidth on the micrometer scale for a precise jet of QDs on the micro-LEDs. To reduce the optical cross-talk effect,a simple lithography method and photoresist are used to fabricate the mold, which consists of a window for QD jetting and a blocking wall for cross-talk reduction. The cross-talk effect of the well-confined QDs in the window is confirmed by a fluorescence microscope, which shows clear separation between QD pixels. A distributed Bragg reflector is covered on the micro-LED array and the QDs' jetted mold to further increase the reuse of UV *** enhanced light emission of the QDs is 5%, 32%, and 23% for blue, green, and red QDs, respectively.

关键词： QDs Optical cross-talk reduction in a quantumdot-based full-color micro-light-emitting-diode display by a lithographic-fabricated photoresist mold

Fluorescence emission from excited molecular ions in intense femtosecond laser fields

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Frontiers of physics 2013年第1期8卷 34-38页

作者： Yun-chen Wang cheng-yin Wu Yuan-Xing Liu Shao-Hua Xu Qi-Huang Gong State Key Laboratory for Mesoscopic Physics Department of Physics Peking University Beijing 100871 China

Strong fluorescence emissions were observed for nitrogen, carbon monoxide, and carbon dioxide molecules in intense femtosecond laser fields. These emissions can be assigned to the transitions of the molecular ions from the excited electronic states to the ground electronic states. The formation mechanisms were discussed and the lifetimes were measured for these excited molecular ions in intense laser fields.

关键词： tunneling ionization molecular orbital fluorescence

Synthesis of novel calix[4]crown telomers and selective extraction of cesium ions

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Chinese Chemical Letters 2007年第3期18卷 263-265页

作者： Hai Bing Li Yuan yin chen De Jun Xiong Jun Yan Zhan Cui Ping Han Key Laboratory of Pesticide ＆ Chemical Biology （CCNU） Ministry of Education College of Chemistry Central China Normal University Wuhan 430079 China Department of Chemistry Wuhan University Wuhan 430072 China

p-tert-Butylcalix[4]diazacrown-4 telomer, which contains hard and soft ion binding sites, was synthesized. It exhibited high selectivity toward cesium ions. The binding sites may complex alkali metal ions selectively.

关键词： Calixcrown Telomer Ion-binding Cesium ions

Cold acclimation induced accumulation of phenolic compounds and freezing tolerance in Ammopiptanthus mongolicus

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Forestry Studies in China 2007年第3期9卷 203-207页

作者： Liu Mei-qin chen Yi-yin Lu Cun-fu Zhang Hui yin Wei-lun College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China

Ammopiptanthus mongolicus, the only freezing tolerant evergreen broad-leaved shrub, local species of the Alashan desert, northwest sand area of China, can survive -30℃ or even lower temperature in winter. In the present study, the secondary products phenolics in A. mongolicus cotyledons were determined to study the effects of phenolics on cold tolerance. Cytochemical localization of phenolics in cotyledon cells was observed by electron microscopy and the content of phenolic compounds was assayed by spectrophotometric measurement. The results showed that the freezing tolerance ofA. mongolicus seedlings increased after acclimation at 2℃ for 14 days, which accompanied the increase of the content of phenolic compounds in cotyledons. Cytochemical observation showed that phenolic deposits were mainly localized in vacuoles and in close proximity to tonoplast, and also in the cytoplasm. The amount and the size of phenolics droplets increased obviously in cytoplasm and vacuoles after cold acclimation, predominantly aggregated along membranes of vacuoles and tonoplast. No phenolic deposits were found in cell walls. As hydrogen- or elec- tron-donating agents, phenolics may protect plant cells against reactive oxygen species formed during chilling or freezing stress and improve the freezing tolerance of cold-acclimated A. mongolicus seedlings.

关键词： phenolic compounds cold acclimation Ammopiptanthus mongolicus

Quasi-Periodic Replacement Policy for a Two-Unit Production System

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Journal of Shanghai Jiaotong university(Science) 2020年第3期25卷 398-406页

作者： GAO Wenke WANG Yu YANG Tianshu JI Hong chen yin College of Energy and Power Engineering Lanzhou University of TechnologyLanzhou 730050China Technical Center R&D DepartmentYEAL Electric Co.Ltd.Changchun 130113China

Maintenance optimization of periodic replacement for mono-unit production system is a widely discussed *** practice,the replacement for a parallel production system with multi-unit served by one repairman is *** replacement action is often implemented in a maintenance window at random,although the replacement period appears as a quasi-periodic *** optimize these maintenance policies,this study analyzes a bivariate quasi-periodic replacement policy (T,W) for a two-unit parallel production system with a maintenance window and one *** unit of the system is replaced when either a failure occurs or a replacement plan comes in the maintenance window,whichever comes *** one of the two units is in the replacement,the other may continue operating or waiting until the replacement is *** optimal replacement window [T*,T* + W*] can be obtained by jointly considering the system's long-running cost rate and the availability using the genetic *** study also introduces three examples of the production system with different types of units to illustrate the proposed policy.

关键词： quasi-periodic replacement policy maintenance window two-unit production system one repairman