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Advances in ethnopharmacology,phytochemistry and pharmacology of Erigerontis Herba and its Chinese medicine prescriptions

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Traditional Medicine Research 2025年第1期10卷 44-61页

作者： Yang Zhou Yuan-Yan Huang Ding-Yue Shang Ying chen Meng tian cheng-Yu Jin Xin Lu Bing-Chun Yan Han-Qing Pang Institute of Translational Medicine School of MedicineYangzhou UniversityYangzhou 225009China Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou UniversityYangzhou 225009China School of Medicine Nanjing University of Chinese MedicineNanjing 210023China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources Guangxi Normal UniversityGuiling 541004China.

Erigerontis Herba(EH),the dried whole plant of Erigeron breviscapus,is well-known for circulating blood,activating meridians to alleviate pain,expelling wind,and clearing away *** has been extensively utilized in southern China for the treatment of stroke hemiplegia,chest stuffiness and pains,rheumatic arthralgia,headache,and *** review focuses on the botany,ethnopharmacology,phytochemistry,pharmacology and toxicity of EH and its related prescriptions to offer new insights for prospective research of *** information about EH was retrieved from ancient records and books,PubMed,China National Knowledge Infrastructure,Chinese Pharmacopoeia,Web of Science,Doctoral and Master’s Theses,and various electronic *** is a member of Compositae family and is mainly grown in southern *** Chinese medicine records that EH has the effects of circulating blood and removing blood stasis,expelling wind,and removing cold,as well as relieving rigidity of muscle and relieving *** now,nearly 200 ingredients have been characterized from EH,including flavonoids,caffeoyls,aromatic acids,coumarins,pentacyclic terpenoids,volatile oil and other *** extracts,EH related prescriptions(Dengzhan Xixin injection,Dengzhan Shengmai capsules,etc.)or compounds(scutellarin,scutellarein,etc.)possessed obvious therapeutic effects of ischemic stroke,cerebral hemorrhage,myocardial infarction,Alzheimer’s disease,diabetes and its complications,gastric cancer,bone,and joint degenerative ***,the major active compound of EH,has been used as a quality *** no obvious toxicity of EH has been *** to its traditional applications,ethnopharmacology,phytochemistry,pharmacology,and toxicity,EH was applied as a valuable herb for clinical application in food and medicine *** several compounds have been shown to possess diverse biological activities,the underlying mechanisms of their actions remain *** fully exploi

关键词： Erigeron breviscapus traditional applications ethnopharmacology phytochemistry pharmacology

高效液相色谱串联质谱法同时检测粪便中三种抗抑郁药物

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中国应用生理学杂志 2020年第6期36卷 668-672页

作者：邵一帆张安琪陈天姣李海北陈郑珊尹静杨栋师丹阳梁永兵程春燕谭蓉孙栋良李君文金敏军事科学院军事医学研究院环境医学与作业医学研究所天津300000

目的:建立同时检测粪便中度洛西汀、氟西汀、艾斯西酞普兰三种抗抑郁药物的高效液相色谱串联质谱(HPLC-MS)方法。方法:样品经正己烷-异丙醇(95:5,v/v)预处理后,以超纯水和乙腈混合液作为流动相进行梯度洗脱,在Agilent ZORBAX SB-C18液... 详细信息

目的:建立同时检测粪便中度洛西汀、氟西汀、艾斯西酞普兰三种抗抑郁药物的高效液相色谱串联质谱(HPLC-MS)方法。方法:样品经正己烷-异丙醇(95:5,v/v)预处理后,以超纯水和乙腈混合液作为流动相进行梯度洗脱,在Agilent ZORBAX SB-C18液相色谱柱(2.1 mm×100 mm,3.5μm)上分离后用电喷雾串联质谱法检测,内标法定量。结果:粪便样品中度洛西汀、氟西汀、艾斯西酞普兰的加标回收率为61.6%~116.5%,精密度为2.80%~12.9%(n=5),线性方程相关系数(r)均大于0.995,检出限分别为0.1μg/g、1μg/g、0.001μg/g,定量限分别为0.5μg/g、2μg/g、0.005μg/g,符合高效液相色谱串联质谱的分析要求。结论:该方法能够简单、准确检测出粪便中度洛西汀、氟西汀、艾斯西酞普兰这三种抗抑郁药的含量。

关键词：粪便高效液相色谱-串联质谱法抗抑郁药物小鼠

小鼠resistin基因表达载体的构建及在RAW264.7细胞的表达

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解剖科学进展 2020年第5期26卷 500-503页

作者：赵鑫月陈添娇于皓李孝琢尚德淑中国医科大学第二临床学院辽宁沈阳110004 中国医科大学生命科学学院发育细胞生物学教研室教育部医学细胞生物学重点实验室辽宁沈阳110122 中国医科大学生命科学学院辽宁沈阳110122

目的构建小鼠resistin基因表达载体,并将其导入小鼠巨噬细胞系RAW264.7细胞中,从而建立能够高表达resistin的单核巨噬细胞系。方法从小鼠脂肪组织提取mRNA,PCR后连接到真核表达载体进行测序验证,同时构建慢病毒载体,感染至RAW264.7细胞... 详细信息

目的构建小鼠resistin基因表达载体,并将其导入小鼠巨噬细胞系RAW264.7细胞中,从而建立能够高表达resistin的单核巨噬细胞系。方法从小鼠脂肪组织提取mRNA,PCR后连接到真核表达载体进行测序验证,同时构建慢病毒载体,感染至RAW264.7细胞中,通过荧光显微镜观察是否转染成功,ELISA方法检测蛋白分泌水平。结果双酶切结果显示构建的质粒中有大小为345bp的resistin插入条带;经过测序及BLAST比对,插入序列与Genebank中resistin的小鼠CDS序列相同,没有移码突变;荧光显微镜观察病毒感染后的RAW264.7细胞,红色荧光比率达到70%;ELISA检测培养的感染细胞上清中resistin分泌显著增加。结论成功获得了能够分泌resistin蛋白的单核/巨噬细胞。

关键词：阿尔茨海默病 resistin基因 Raw264.7细胞病毒感染

Item Ownership Relationship Semantic Learning Strategy for Personalized Service Robot

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International Journal of Automation and computing 2020年第3期17卷 390-402页

作者： Hao Wu Zhao-Wei chen Guo-Hui tian Qing Ma Meng-Lin jiao School of Control Science and Engineering Shandong UniversityJinan 250061China

In order to satisfy the robotic personalized service requirements that can select exclusive items to perform inference and planning according to different service individuals,the service robots need to have the ability to independently obtain the ownership relationship between humans and their carrying *** this work,we present a novel semantic learning strategy for item ***,a human-carrying-items detection network based on human posture estimation and object detection model is ***,the transferred convolutional neural network is used to extract the characteristics of the objects and the back-end classifier to recognize the object *** the same time,the face detection and recognition model are used to identify the service ***,on the basis of the former two,the active learning of ownership items is *** experimental results show that the proposed ownership semantic learning strategy can determine the ownership relationship of private goods accurately and *** solution of this problem can improve the intelligence level of robot life service.

关键词： Service robot ownership relationship convolutional neural network human-object spatial relationship personalized service

Ultra‑High Sensitivity Anisotropic Piezoelectric Sensors for Structural Health Monitoring and Robotic Perception

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Nano-Micro Letters 2025年第2期17卷 432-446页

作者： Hao Yin Yanting Li Zhiying tian Qichao Li chenhui Jiang Enfu Liang Yiping Guo State Key Laboratory of Metal Matrix Composites School of Materials Science and EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China Beijing Vacuum Electronics Research Institute Beijing 100015People’s Republic of China Fundamental Science On Vibration Shock and Noise LaboratoryState Key Laboratory of Mechanical System and VibrationSchool of Mechanical EngineeringShanghai Jiao Tong UniversityShanghai 200240People’s Republic of China

Monitoring minuscule mechanical signals,both in magnitude and direction,is imperative in many application scenarios,e.g.,structural health monitoring and robotic sensing ***,the piezoelectric sensor struggles to satisfy the requirements for directional recognition due to the limited piezoelectric coefficient matrix,and achieving sensitivity for detecting micrometer-scale deformations is also ***,we develop a vector sensor composed of lead zirconate titanate-electronic grade glass fiber composite filaments with oriented arrangement,capable of detecting minute anisotropic *** as-prepared vector sensor can identify the deformation directions even when subjected to an unprecedented nominal strain of 0.06%,thereby enabling its utility in accurately discerning the 5μm-height wrinkles in thin films and in monitoring human pulse *** ultra-high sensitivity is attributed to the formation of porous ferroelectret and the efficient load transfer efficiency of continuous lead zirconate titanate ***,when integrated with machine learning techniques,the sensor’s capability to recognize multi-signals enables it to differentiate between 10 types of fine textures with 100%*** structural design in piezoelectric devices enables a more comprehensive perception of mechanical stimuli,offering a novel perspective for enhancing recognition accuracy.

关键词： Flexible piezoelectric filaments Anisotropic Ultra-high sensitivity Structural health detection Texture recognition

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重组人源超氧化物歧化酶研究进展

中国医药生物技术 2019年第4期14卷 352-357,321页

作者：李星论巩婷陈晶晶陈天娇乔云明杨金玲朱平中国医学科学院北京协和医学院天然药物活性物质与功能国家重点实验室/国家卫健委天然药物生物合成重点实验室

超氧化物歧化酶(superoxide dismutase,SOD)是动物、植物和微生物中普遍存在的一类酶,是生物抗氧化系统的第一道防线,也是体内唯一能够特异性清除氧自由基的抗氧化酶。SOD为金属酶,按其结合的金属离子种类不同,目前已知的SOD主要分为三... 详细信息

超氧化物歧化酶(superoxide dismutase,SOD)是动物、植物和微生物中普遍存在的一类酶,是生物抗氧化系统的第一道防线,也是体内唯一能够特异性清除氧自由基的抗氧化酶。SOD为金属酶,按其结合的金属离子种类不同,目前已知的SOD主要分为三类,即Cu/Zn-SOD(SOD1)、Mn-SOD(SOD2)和Fe-SOD(SOD3)[1]。人体内外源化合物在各种酶的作用下会产生氧自由基,氧自由基在SOD的催化作用下转变为过氧化氢,体内的过氧化氢酶(CAT)和过氧化物酶(POD)会立即将其分解为完全无害的水(图1)。

关键词：超氧化物歧化酶清除氧自由基 SOD1 过氧化氢酶人源重组 Fe-SOD Mn-SOD

Corrosion and wear properties of in situ(TiB+TiC)/TA15 composites with a high volume percentage of reinforcement

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China Foundry 2023年第5期20卷 423-431页

作者： Wei-long Wang Kun Shi Jun Zhao Shi-bing Liu jiao-jiao cheng Wei-chen Qiu tian-yu Liu Zhi-yong Zhang Shenyang Research Institute of Foundry Co. Ltd.CAMShenyang 110022China National Key Laboratory of Advanced Casting Technologies Shenyang 110022China

The in situ(TiC+TiB)/TA15 composites with different volume percentages of reinforcement(10%,15%,20%and 25%)were prepared by water-cooled copper crucible vacuum suspension melting *** structures and compositions of the TA15 alloy and its composites were analyzed by XRD and EDS,and their electrochemical corrosion behaviors in the 3.5%NaCl solution were *** wear testing was conducted using a reciprocating ball-on-disc wear tester under a 10 N *** show that the in situ fibrous TiB phase and the granular TiC phase are uniformly distributed on the composite *** microhardness can reach up to 531 HV as 25vol.%TiC+TiB reinforcement is *** with the TA15 alloy,the volume wear rate decreases from(2.21±0.07)×10^(-4)to(1.75±0.07)×10^(-4)mm^(3)·N^(-1)·m^(-1)by adding 15vol.%TiC+TiB reinforcement,and the wear mechanism is adhesive *** the volume percentage of the reinforcement phase reaches 25%,the volume wear rate increases from(1.75±0.07)×10^(-4)to(2.41±0.07)×10^(-4)mm^(3)·N^(-1)·m^(-1),and the wear mechanism changes into abrasive *** volume loss resulted by the interaction between corrosion and wear accounts for more than 27%of the total wear *** volume loss due to wear-induced corrosion changes from 1.94%to 4.06%with different additions of *** volume loss caused by corrosion-induced wear initially increases from 24.08%to 26.90%as the reinforcement increases from 0 to 15%due to the increase of corrosion potential,and then decreases from 26.90%to 25.68%as the reinforcement increases from 15%to 25%due to the peeling of TiC phase.

关键词： titanium matrix composites high volume percentage corrosion wear wear mechanism

Current Status of Targets and Assays for Anti-HIV Drug Screening

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Virologica Sinica 2007年第6期22卷 476-485页

作者： Ren-rong tian Qing-jiao LIAO Xu-lin chen State Key Laboratory of Virology Wuhan Institute of Virology Chinese Academy of Sciences Wuhan 430071 China

HIV/AIDS is one of the most serious public health challenges globally. Despite the great efforts that are being devoted to prevent,treat and to better understand the disease,it is one of the main causes of morbidity and mortality worldwide. Currently,there are 30 drugs or combinations of drugs approved by FDA. Because of the side-effects,price and drug resistance,it is essential to discover new targets,to develop new technology and to find new anti-HIV drugs. This review summarizes the major targets and assays currently used in anti-HIV drug screening.

关键词： Anti-HIV Targets Assays Drug screening

TET2在帕金森病细胞模型中的表达及机制研究

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现代生物医学进展 2020年第4期20卷 638-642,633页

作者：吴婷婷刘特陈天骄陈雅静李璇朱潇颖吴云成上海交通大学附属第一人民医院神经内科上海200080 上海中医药大学中医老年医学研究所中心实验室上海200031

目的:迄今为止,帕金森病(PD)发生的分子机制尚未完全阐明,本研究旨在体外细胞模型中寻找PD新型表观遗传标志物,探索其发病机制。方法:本次研究使用的细胞为神经母细胞瘤细胞系SH-SY5Y。首先,我们用CCK-8检测细胞活力,选取合适浓度的MPP... 详细信息

目的:迄今为止,帕金森病(PD)发生的分子机制尚未完全阐明,本研究旨在体外细胞模型中寻找PD新型表观遗传标志物,探索其发病机制。方法:本次研究使用的细胞为神经母细胞瘤细胞系SH-SY5Y。首先,我们用CCK-8检测细胞活力,选取合适浓度的MPP+构建PD细胞损伤模型。再用PBS和MPP+分别处理SH-SY5Y细胞,用RT-qPCR检测了几个甲基化酶与去甲基化酶DNMT1,DNMT3A,DNMT3B及TET1, TET2, TET3的mRNA的表达水平,并用蛋白印迹检测TET2蛋白水平,免疫荧光检测了TET2蛋白定位。进一步用慢病毒转染SH-SY5Y细胞敲低TET2后,检测细胞增殖。结果:本研究发现,MPP+对SH-SY5Y细胞增殖的抑制具有时间与浓度依赖性,我们最终选择2.5 mM MPP+作为后续的细胞处理浓度。与对照组相比,MPP+处理细胞TET2的mRNA及蛋白水平表达均增加,且蛋白进入细胞核增加;同时发现,敲低TET2表达可以延缓MPP+对SH-SY5Y细胞增殖的抑制作用。结论:在当前的研究中,我们报道了TET2蛋白可能是PD新型的表观遗传学标志物,提示我们将来也许可以使用TET2抑制剂来治疗PD,因此本研究有可能为PD提供新的治疗方向和靶点。

关键词：帕金森病 TET2 DNA羟甲基化中脑多巴胺能神经元