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Characterization of Bacterial Community Structure and Diversity in Rhizosphere Soils of Three Plants in Rapidly Changing Salt Marshes Using 16S rDNA

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Pedosphere 2007年第5期17卷 545-556页

作者： WANG Meng chen Jia-Kuan li bo Coastal Ecosystems Research Station of Yangtze River Estuary Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering Institute of Biodiversity Science Fudan University 220 Handan Road Shanghai 200433 （China）.

The structure and diversity of the bacterial communities in rhizosphere soils of native Phragmites australis and Scirpus rnariqueter and alien Spartina alterniflora in the Yangtze River Estuary were investigated by constructing 16S ribosomal DNA （rDNA） clone libraries. The bacterial diversity was quantified by placing the clones into operational taxonomic unit （OTU） groups at the level of sequence similarity of 〉 97%. Phylogenetic analysis of the resulting 398 clone sequences indicated a high diversity of bacteria in the rhizosphere soils of these plants. The members of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria of the phylum Proteobacteria were the most abundant in rhizobacteria. Chao 1 nonpaxametric diversity estimator coupled with the reciprocal of Simpson＇s index （l/D） was applied to sequence data obtained from each library to evaluate total sequence diversity and quantitatively compare the level of dominance. The results showed that Phragmites, Scirpus, and Spartina rhizosphere soils contained 200, 668, and 382 OTUs, respectively. The bacterial communities in the Spartina and Phragraites rhizosphere soils displayed species dominance revealed by 1/D, whereas the bacterial community in Scirpus rhizosphere soil had uniform distributions of species abundance. Overall, analysis of 16S rDNA clone libraries from the rhizosphere soils indicates that the changes in bacterial composition may occur concomitantly with the shift of species composition in plant communities.

关键词： 16S rDNA bacterial diversity plant succession rhizosphere salt marshes

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Characteristic of bond Zone between Powder Surfacing Layer and Base Metal

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Journal of Iron and Steel Research International 2004年第1期11卷 58-62页

作者： SHAN Ji-guo ZHANG Di REN Jia-lie chen bo-li Tsinghua University

The characteristic of the bond zone between Ni-based alloy light beam surfacing layer(SL)and base metal(BM)was investigated by scanning electron microscope,energy dispersive spectrometer and X-ray *** results show that the bond zone,which consists ofγ-Ni orγ-(Fe,Ni)planar crystal band close to SL andα-Fe bright band close to heat affected zone(HAZ),is actually the transition zone of composition and microstructure between SL and HAZ,and the metallurgical bond interface lies between theα-Fe bright band and *** the increase of light beam heat input from 2kJ/mm to 4kJ/mm,the width of the bond zone increases from 4μm to 15μm,and the morphology of bond interface changes from zigzag to *** formation of bond interface indicates the formation of reliable metallurgical bond between SL and BM.

关键词： light beam powder surfacing layer bond zone characteristic

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Preparation of n-Atom GHZ State and Implementation of Quantum-State Transfer via Cavity Decay

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Communications in Theoretical Physics 2008年第3期49卷 655-658页

作者： chen li-bo DU Qian-Hua liN Gong-Wei chen Zhi-Hua liN Xiu-Min School of Physics and Optoelectronics Technology Fujian Normal University Fuzhou 350007 China

We propose schemes to prepare n-atom Greenberger-Horn-Zeilinger （GHZ） state via two-sided cavities interacting with single-photon pulses, and achieve quantum state transfer （QST） from one atom to another atom. Entanglement particle pair and the control of coupling between qu bits are of no need in the QST process. Some practical quantum noises only decrease the success probabilities of the schemes but have no influence on the fidelity of prepared state. In addition, the success probabilities of our schemes are close to unity in the ideal case.

关键词： GHZ state quantum state transfer cavity

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Changes in percentage of lymphocyte subsets after ^(131)I treatment in patients with differentiated thyroid cancer

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Nuclear Science and Techniques 2005年第4期16卷 214-216页

作者： LUO Quan-Yong chen li-bo YU Yong-li LU Han-Kui ZHU Rui-Sen Department of Nuclear Medicine Shanghai Sixth People＇s Hospital. Shanghai Jiaotong University Shanghai 200233

To monitor the extent and the duration of lymphocyte subset changes in patients with thyroid carcinoma undergoing therapeutic 131I administration, the percentage of lymphocyte subsets were serially analyzed before and after 131I treatment. In patients who received 1850 MBq of 131I for ablation of thyroid remnants, only for NK cells and B cells showed a significant reduction. In patients received 3700 MBq of 131I for treatment of local lymph node me-tastases, NK cells, B cells and CD4+ were found decreased. In patients received 7400 MBq of 131I for treatment of distant metastases, NK cells, B cells and CD4+ and CD8+ were all affected. However, there is no significant reduc-tion compared to the baseline in the percentage of all lymphocyte subsets three months after 131I treatment. The re-sults show that the sensitivity of lymphocytes to 131I internal radiation depends upon lymphocyte phenotype and 131I activity. The immunosuppression effects are temporary and reversible.

关键词：淋巴细胞 ^131I 甲状腺癌症放射性疗法抑制免抑反应

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Comparative Proteomic Analysis of Human Benign Prostatic Hyperplastic Tissue Before and After Irradiation with Radioactive Nuclide

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Chemical Research in Chinese Universities 2010年第1期26卷 81-85页

作者： chen li-bo ZHAO Jie ZHAO Guo-qing XU Yan-li li Jun GAO Shi MA Qing-jie China-Japan Union Hospital of Jilin University Changehun 130033 P. R. China Medical College of Hebei Engineering University Handan 056029 P. R. China

The expressed proteins were extracted from human benign prostatic hyperplastic tissues obtained with transurethral resection of the prostate before and after their irradiation with radioactive nuclide. The proteins were separated by two-dimensional gel electrophoresis and analyzed by mass spectrometry. Four proteins were differentially expressed and were identified with a database search. Three were associated with the regulation of cell motion and one was lactate dehydrogenase B, which plays an important role in the process of cell energy metabolism and cell state changes in tissue. The comparative proteomic analysis indicates that after irradiation with radioactive nuclide, changes occur in human benign prostatic hyperplastic cells, and associated proteins are expressed. Analysis of these proteins should help to identify the mechanism involved when human prostatic hyperplasia is treated by irradiation with radioactive nuclide.

关键词： Prostatic hyperplasia Radioactivity Nuclide Proteome

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Development of a large-scale isolation chamber system for the safe and humane care of medium-sized laboratory animals harboring infectious diseases

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第10期11卷 771-778页

作者： Xin PAN Jian-cheng QI Ming LONG Hao liANG Xiao chen Han li Guang-bo li Hao ZHENG Department of Microbiology the Second Military Medical University Shanghai 200433 China

The close phylogenetic relationship between humans and non-human primates makes non-human primates an irreplaceable model for the study of human infectious *** this study,we describe the development of a large-scale automatic multi-functional isolation chamber for use with medium-sized laboratory animals carrying infectious *** isolation chamber,including the transfer chain,disinfection chain,negative air pressure isolation system,animal welfare system,and the automated system,is designed to meet all biological safety *** create an internal chamber environment that is completely isolated from the exterior,variable frequency drive blowers are used in the air-intake and air-exhaust system,precisely controlling the filtered air flow and providing an air-barrier protection.A double door transfer port is used to transfer material between the interior of the isolation chamber and the outside.A peracetic acid sterilizer and its associated pipeline allow for complete disinfection of the isolation *** of the isolation chamber parameters can be automatically controlled by a programmable computerized menu,allowing for work with different animals in different-sized cages depending on the research *** large-scale multi-functional isolation chamber provides a useful and safe system for working with infectious medium-sized laboratory animals in high-level bio-safety laboratories.

关键词： Multi-function Medium-sized animal Isolation chamber Biological safety

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Effect of gravel content on the detachment of colluvial deposits in Benggang

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Journal of Mountain Science 2022年第11期19卷 3088-3104页

作者： ZHANG li-ting SHUAI Fang chen li-bo HUANG Yan-he liN Jin-shi ZHANG Yue GE Hong-li JIANG Fang-shi Jinshan Soil and Water Conservation Research Center Fujian Agriculture and Forestry UniversityFuzhou 350002China

Accurately calculating detachment capacity is the most fundamental issue when establishing a soil erosion process *** deposits of Benggang are typical soil-gravel mixtures,whereas the understanding of the soil detachment of colluvial deposits is *** work investigated the effects of the gravel contents on the soil detachment capacity of colluvial deposits and its hydrodynamic *** colluvial sample was collected in Anxi County,Fujian Province,Southeast China,and a small-sample scouring test was *** slope steepness ranged from 18%to 84%,unit discharge ranged from 0.56×10^(-3)to 2.22×10^(-3)m^(2)s^(-1),and gravel content ranged from 0%to 70%.The results indicated that the gravel content is the primary factor that influences the detachment capacity,followed by the discharge and then the *** detachment capacity trend with the gravel content varied over different slopes and *** power represents the best hydrodynamic parameter for modelling the detachment capacity of colluvial deposits and can be used to establish a fitting equation for the colluvium together with the mean weight diameter(MWD)(Nash-Sutcliffe efficiency(NSE)=0.96).As the gravel content increased,the soil erodibility parameters increased several folds,in some cases more than 10 folds,mainly because the soil shear strength decreased ***,as the gravel content increased,the gravel specific surface area increased,the obstruction of gravel to runoff increased,and the energy needed for runoff to overcome gravel obstruction increased,leading to 2-3 folds higher critical shear stress of runoff for soilgravel mixtures compared with pure *** summary,gravel can influence the detachment capacity by changing the soil properties,and the gravel content also affects the relationship between soil detachment capacity and the hydrodynamic *** findings deepen the understanding of the influence of gravel on soil erosion and provide a basis for

关键词： Hydraulic erosion Hydrodynamics Gravel content Soil detachment Erosion resistance Benggang

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Inhibition of M-MuLV reverse transcriptase activity by fullerene derivatives

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Chinese Science Bulletin 2006年第20期51卷 2550-2552页

作者： MENG Xianmei chen Zhe li bo ZHANG Yufei ZHAO Dongxu YANG Xinlin School of life Science and Technology Beijing Institute of TechnologyBeijing 100081 China

Influence of fullerene (C60) derivatives on M-MuLV reverse transcriptase activity is investi- gated. Two water-soluble fullerene derivatives, fullerol (C60(OH)23-24) and trimalonic acid C60 (TMA C60, C63(COOH)6) are used in the experiments and their effects on in vitro reverse transcription-polymerase chain reaction (RT-PCR) of β-actin mRNA in HeLa cells are determined. PCR products are detected by agarose gel electrophoresis. It is found that the amounts of PCR products decrease with addition of either of two fullerene derivatives to RT reaction mixture. The inhibition of fullerene derivatives is dose-dependent, and IC50 values of fullerol and TMA C60 are 0.089 and 0.039 mmol/L, respectively. The amount of PCR products obtained by direct addition of fullerol or TMA C60 to PCR are greater than those obtained by addition of the equivalent amount of fullerol or TMA C60 to RT, indicating an inhibitory ef- fect of fullerol or TMA C60 on RT. The compensative experiment of M-MuLV reverse transcriptase shows that increasing enzyme amounts can antagonize the activity of fullerol or TMA C60. These results imply that fullerenes can inhibit M-MuLV reverse transcriptase activity, with the inhibition of TMA C60 slightly stronger than fullerol, and that their potential in treatment of diseases induced by RNA viruses such as leukemia virus needs further investigation.

关键词： M-MuLV 反转录酶抑制作用水溶性丙二酸

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Implementing Entanglement Swapping and Generating Multiphoton Entanglement in Cavity QED

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Communications in Theoretical Physics 2008年第6期49卷 1483-1486页

作者： liN Gong-Wei chen li-bo DU Qian-Hua LAN Jie-Qin liN Xiu-Min School of Physics and Optoelectronics Technology Fujian Normal University Fuzhou 350007 China

Based on the input-output relation of a one-sided cavity, we propose an experimental scheme to implement entanglement swapping with photons. In the ideal case, the successful probability of the scheme approaches unity. In addition, the protocol can be extended to establish multiphoton entanglement among distant users in a communication network.

关键词： entanglement swapping multiphoton entanglement

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How to Apply TPM in Equipment Management for Chinese Enterprises

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Chinese Business Review 2011年第2期10卷 137-145页

作者： chen li-xia MENG bo Changchun University of Science and Technology Changchun China

The paper studies an effective way for Chinese enterprises to implement Total Productive Maintenance （TPM） to cut cost and increase efficiency under the impact of financial crisis. It first reviews the definition and objectives of TPM, clarifies how TPM supports lean objectives, and suggests seven steps and essential elements for Chinese enterprises to implement TPM systematically and successfully. The paper also puts the steps and essential elements into practice in a Chinese enterprise, which are proven effective and we hope the proposals are helpful for Chinese enterprises to change their extensive operation in equipment management.

关键词： TPM autonomous maintenance seven steps essential elements

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