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Comparative Analyses of Proteome Complement Between Worker Bee Larvae of High Royal Jelly Producing Bees (A. m. ligustica) and Carniolian Bees (A. m. carnica)

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Agricultural Sciences in China 2009年第10期8卷 1219-1227页

作者： chen jian LI jian-ke Institute of Apicultural Research Chinese Academy of Agricultural Sciences~Key Laboratory of Pollinating Insect Biology Ministry ofAgriculture Beijing 100093 P.R China

This study is to compare the protein composition of the high royal jelly producing bee （A. m. ligustica） with that of Carniolian bee （A. m. carnica） during their worker larval developmental stage. The experiment was carried out by two- dimensional gel electrophoresis. The results showed that significant higher numbers of total proteins （283） were detected in larvae of high royal jelly producing bees （Jelly bee） than those of Carniolian bees （152） on 2-d-old larvae. Among them, 110 proteins were presented on both strains of bee larvae, whereas 173 proteins were specific to larvae of Jelly bees, and 42 proteins were exclusive to Carniolian larvae. However, on the 4th d, a significant higher number of total proteins （290） were detected in larvae of Jelly bees than those of Carniolian bees （240）, 163 proteins resolved to both bee larvae, and 127 proteins were specific to Jelly bees and 77 proteins to Camiolian bees. Until the 6th d, also a significant higher number of total proteins （236） were detected in larvae of Jelly bees than those of Carniolian bees （180）, 132 proteins were constantly expressed in two bee larvae, whereas 104 and 48 proteins are unique to Jelly bee and Carniolian bee larvae, respectively. We tentatively concluded that the metabolic rate and gene expression of Jelly bees larvae is higher than those of Carniolian bees based proteins detected as total proteins and proteins specific to each stage of two strains of bee larvae. Proteins constantly expressed on 3 stages of larval development with some significant differences between two bee strains, and proteins unique to each stage expressed differences in term of quality and quantity, indicating that larval development needed house keeping and specific proteins to regulate its growth at different development phage, but the expression mold is different between two strains of larval development.

关键词： honeybee (Apis mellifera) worker bee larva two-dimensional gel electrophoresis proteome

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Research of Temperature Tracer Method to Detect Tubular Leakage Passage in Earth-Dam

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Journal of China University of Mining and Technology 2006年第3期16卷 353-358页

作者： WANG Xin-jian chen jian-sheng Geotechnical Institute Hohai University Nanjing Jiangsu 210098 China

The location, intensity and scope of concentrated leakage must be determined in order to repair earth-Dam scoured by the leakage. In this paper, firstly, heat tracer theory and distribution laws of temperature in soil body with leakage are discussed. Then temperature tracer model is established according to stable heat conduction theory. In such model, the concentrated seepage passage is simplified into a circular pipe as a boundary condition. The location, scope and flow-velocity of the concentrated leakage are estimated via ichnography of the lowest temperature based on temperature data from detecting wells by quantitative computation and qualitative analysis. In case study, the distribution characteristic of temperature (including temperature data of water in reservoir, drainage pipes and tail pond) can be interpreted by this model. A modified model is also set up, applied for detected data at different cross-sections of the leakage passage, in which the temperature data are rectified according to distances from data locations to calculating section. Finally, the model is solved by numerical iterative method, and the possible error of this theoretical model is discussed. The permeability coefficient in leakage area is identical with that of normal soil in magnitude after anti-seepage repairing accomplished, which indicates this model is effective.

关键词： seepage concentrated leakage passage heat conduction tracer method temperature field earth-dam

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NUMERICAL SIMULATION OF THE ENERGY DISSIPATION CHARACTERISTICS IN STILLING BASIN OF MULTI-HORIZONTAL SUBMERGED JETS

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Journal of Hydrodynamics 2010年第5期22卷 732-741页

作者： chen jian-gang ZHANG jian-min XU Wei-lin WANG Yu-rong State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University Chengdu 610065 China

3-D numerical simulation was carried out for the water flow in a stilling basin with multi-horizontal submerged jets by using two different turbulence models, namely, the VOF RNG k-ε and Mixture RNG k-ε turbulence models. The calculated water depth, velocity profile and pressure distribution are in good agreement with the data obtained in experiments. It indicates that the numerical simulation can effectively be used to study the water flow movement and the energy dissipation mechanism. The numerical simulation results show that the turbulent kinetic energy distribution obtained by using the Mixture turbulence model covers a region about 18% larger than that calculated by using the VOF turbulence model, and is in better agreement with the actual situation. Furthermore, the Mixture turbulence model is better than the VOF turbulence model in calculating the air entrainment.

关键词： multi-horizontal submerged jets numerical simulation VOF method Mixture method hydraulic characteristics stilling basin

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SCALE EFFECTS OF AIR-WATER FLOWS IN STILLING BASIN OF MULTI-HORIZONTAL SUBMERGED JETS

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Journal of Hydrodynamics 2010年第6期22卷 788-795页

作者： chen jian-gang ZHANG jian-min XU Wei-lin WANG Yu-rong State Key Laboratory of Hydraulics and Mountain River Engineering Sichuan University Chengdu 610065China

A series of experiments were carried out on multi-horizontal submerged jets with four different model scales of 1：36, 1：57, 1：80, 1：200. In routine tests, scale effects have to be considered, due to complex vortex structure and strong air entrainment in stilling basin. Our focus was laid on measuring and analyzing the time-averaged pressure distribution, water depth and closed-to-bed velocity in the stilling basin of multi-horizontal submerged jets. The experiments results show that the model scale has but a slight effect on the time-averaged hydraulic characteristics in the stilling basin of multi-horizontal submerged jets, which indicates that the results of time-averaged hydraulic characteristics for a normal pressure model are reliable. However, the scale effects of air entrainment, fluctuation pressure and vortex structure call for further investigations in order to make the results of experiments serve as scientific references for practical engineering.

关键词： multi-horizontal submerged jets Xiangjiaba hydropower station scale effects experimental investigation hydraulic characteristics

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Melanoma differentiation-associated gene-7, MDA-7/IL-24, selectively induces growth suppression, apoptosis in human hepatocellular carcinoma cell line HepG2 by replication-incompetent adenovirus vector

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World Journal of Gastroenterology 2006年第11期12卷 1774-1779页

作者： Cong-Jun Wang Xin-Bo Xue Ji-Lin Yi Kun chen jian-WeiZheng jian Wang jian-Ping Zeng Rong-Hua Xu Department of Biliary and Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 Hubei Province China

AIM： To investigate the effect of replication-incompetent adenovirus vector expressing MDA-7/IL-24 on tumor growth and apoptosis in human hepatocellular carcinoma （HCC） cell line HepG2 and normal liver cell line L02. METHODS： We constructed the recombinant replication-incornpetent ***-7 virus vector and infected it into the human HCC cell line HepG2 and normal liver cell line L02. RT-PCR was performed to detect the rnRNA expressing in cells, by ELISA was used to detect MDA-7/IL-24 protein expression in the culture supernatant. The effect of apoptosis induced by ***-7 was confirmed by Hoechst staining and flow cytometry assay with Annexin-V and PI staining. MTT assay was used to determine growth inhibition of HepG2 cells, and cell-cycle and hypodiploidy analyses were performed by flow cytometry. RESULTS： Recombinant replication-defective virus expressing MDA-7/IL-24 was constructed successfully. RTPCR showed that the ***-7 could mediate the expression of the exogenous gene MDA-7/IL-24 into HepG2 and L02. The concentration of MDA-7/IL-24 protein in supernatant was 130 pg/mL and 110 pg/mL in ***-7-infected L02 and HepG2 ceils, respectively. ***-7 infection obviously induced apoptosis （from 2.604±0.72% to 33.64±13.2%, P=0.00012） and growth suppression in HepG2 （inhibition ratio IR=68%） and an increase in the percentage of specific cancer cell types at the G2/M phase of the cell cycle （from 6.44% to 32.29%, P〈 0.01）, but not in L02 ***： These results confirm selectively induction of apoptosis and growth suppression by the mda-7/ IL-24 gene with replication-incompetent adenovirus vector in human hepatocellular carcinoma cell line HepG2.

关键词： Cancer gene therapy Hepatocellular carcinoma (HCC) Apoptosis Growth suppression MDA-7/IL-24

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Linearly McCoy Rings and Their Generalizations

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Communications in Mathematical Research 2010年第2期26卷 159-175页

作者： CUI jian chen jian-LONG Department of Mathematics Southeast University Nanjing 210096

A ring R is called linearly McCoy if whenever linear polynomials f（x）, g（x） e R[x]／{0） satisfy f（x）g（x）： O, then there exist nonzero elements r, s ∈ R such that f（x）r ： sg（x） =0. For a ring endomorphism α, we introduced the notion of α-skew linearly McCoy rings by considering the polynomials in the skew polynomial ring R[x; α] in place of the ring R[x]. A number of properties of this generalization are established and extension properties of α-skew linearly McCoy rings are given.

关键词： linearly McCoy ring a-skew linearly McCoy ring polynomial ring,matrix ring

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Genetic Analysis of the Resistance to Aspergillus flavus Infection in Maize (Zea mays L.)

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Agricultural Sciences in China 2009年第6期8卷 761-765页

作者： DENG De-xiang jianG Si-xia WANG Yi-jun BIAN Yun-long chen jian-jian JIA Bo jiangsu Province Key Laboratory of Crop Genetics and Physiology~Key Laboratory of Plant Functional Genomics Ministry of Education Yangzhou University Yangzhou 225009 P.R. China Yangzhou Environment and Resources Technical College Yangzhou 225009 P.R. China

Maize （Zea mays L.）, one of main crops in the world, is easily susceptible to Aspergillusflavus （Link： fr） infection, resulting huge loses worldwide. Breeding for A. flavus resistance has been proved an efficient way to solve the problem of aflatoxin contamination. Genetic analysis of the sources of resistance to *** in maize is necessary for this purpose. The complete diallel crosses of 6 inbred lines with different resistance to *** infection were implemented. Inoculation categorical data of each cross were analyzed with the additive-dominant and additive-dominant-epitasis genetic models. Results indicated some crosses fitted the 2 major genes with additive-dominant-epitasis genetic model. Others fitted the major gene and polygene mixed model. Moreover, the additive, dominant, and epitasis effects varied in crosses. The *** resistance was controlled by both major gene and polygene.

关键词： maize Aspergillus flavus resistance genetic model

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Synthesis of poly（lactide-co-glycolide） with high viscosity-average molecular weight

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Journal of Chemistry and Chemical Engineering 2009年第4期3卷 18-25页

作者： FAN Xin chen jian RUAN jian-ming ZHOU Zhong-chen ZOU jian-peng State Key Laboratory of Powder Metallurgy Central South University Changsha 410083 China

In the present paper, poly（lactide-co-glycolide）（PLGA） with high viscosity-average molecular weight was fabricated with L-lactide （LLA） and glycolide （GA） as monomers via opening-ring polymerization. Fourier transform Infrared （FTIR） spectroscopy, ~H-NMR, X-ray diffraction （XRD） and scanning electron microscopy （SEM） were applied to characterize the products. Viscosity method was used to determine the viscosity-average molecular weight of the PLGA copolymers. Moreover, the mechanical strength of PLGA was investigated. The results showed that the highly pure LLA and GA were prepared. The as-fabricated PLGA was typical semicrystalline copolymer with the crystallinity of 18.72%. Both viscosity-average molecular weight and mechanical strength of PLGA decreased gradually with the increase of GA content. In addition, the higher the viscosity-average molecular weight was, the more the microcracks and holes on the cross-section of PLGA copolymer materials were, the smoother the cross-sections were.

关键词： high molecular weight PLGA synthesis characterization

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Mining Cross-Transaction Web Usage Patterns

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通讯和计算机（中英文版） 2005年第5期2卷 6-11,81页

作者： jian chen jian Yin Jin Huang Liangyi Ou Department of Computer Science Sun Yat-sen University Guangzhou 510275 China

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Preparation and Photoconductivity of Fe-Phthalocyanine Derivation and C_(60) Composite Films

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Journal of Shanghai University(English Edition) 2001年第2期5卷 156-159页

作者： chen jian ming, ZHANG jian cheng, SHEN Yue, LIU Xiu hong, YANG Lin hong School of Material Science and Engineering, Shanghai University, Shanghai, 200072, China School of Material Science and Engineering Shanghai University Shanghai China

Fe (III) 2,9,16,23 tertracarboxy phthalocyanine (Fe (III) taPc) was synthesized, and bonded to polystyrene (PS) with covalence by Friedel Crafts reaction to form a new polymer (Fe (III) taPc PS) (polymer (II)). UV VIS and Infrared spectra indicated that Fe (III) taPc was successfully bonded to PS. A photoreceptor device of sandwich structure consisting of alternate layers of polymer (II) and fullerene (C 60 ) was prepared. The experiment results show that the photoconductivity of the photoreceptor is higher than that of the single layer film of polymer (II) or C 60 , because of the charge transfer effect between the layers.

关键词： phthalocyanine polystyrene C 60 photoconductivity composite films

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