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Experimental Study of the Differentiation of Mesenchymal stem Cells ( MSCs) after Indirect Co-culture with Ligament Fibroblasts

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生物医学工程学杂志 2005年第S1期22卷 143-144页

作者： Lei ZHANG~(1.2. Xiong WANG~(2.) Huai-qing chen~1.({2. of Biomedical Engineering, West China Medical Center, sichuan University, chengdu 610041,China)2.Equipe Mécanique et Ingénierie Cellulaire et Tissulaire, Institut National Polytechnique de Lorraine, 54500 Vandoeuvre-lès-Nancy, France) institute of Biomedical Engineering West China Medical Center Sichuan University Chengdu 610041 China Equipe Mecanique et Ingénierie Cellulaire et Tissulaire Institut National Polytechnique de Lorraine 54500 Vandoeuvre-lès-Nancy France Equipe Mecanique et Ingénierie Cellulaire et Tissulaire Institut National Polytechnique de Lorraine 54500 Vandoeuvre-lès-Nancy France institute of Biomedical Engineering West China Medical Center Sichuan University Chengdu 610041 China

1.IntroductionToday tissue engineering is known to be a new technique which, based on biological and physical manipulations on cells, aims at the regeneration and reconstruction of tissues or organs in vitro. To construct active ligament tissue by this technique, the following three key points should be considered: choice of cells, scaffold, and the mechanical conditions in vitro. But the studies on ligament cell biology are still in an early stage[1..

关键词： MSCs In after Indirect Co-culture with Ligament Fibroblasts Experimental Study of the Differentiation of Mesenchymal stem Cells stem

An ultrafast rechargeable hybrid potassium dual-ion capacitor based on carbon quantum dot@ultrathin carbon film cathode

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Rare Metals 2024年第10期43卷 5070-5081页

作者： Zhao-Meng liu Da Wang Shang-Zhuo Li {1.-Song Lai Dong-Run Yang Lu-Kang Zhao Jian-Jia Mu Xuan-{2. Wang Xuan-Wen Gao Wen-Bin Luo institute for Energy Electrochemistry and Urban Mines Metallurgy School of MetallurgyNortheastern University Key Laboratory of Advanced Energy Materials Chemistry(Ministry of Education) Nankai University

Ultrafast rechargeable hybrid potassium dualion capacitors （HPDICs） were designed by employing carbon quantum dot@ultrathin carbon film （CQD@CF） as the cathode *** designed CQD@CF is selfassembled by a simple catalytic graphitization route followed by an acid leaching *** special composite features a large adsorption interface,a remarkable anion hybrid storage capability and outstanding structure *** electrochemical performance of CQD@CF composite is fully tapped in a half-cell system at the operating voltage between 1.4 and 4.5 V,achieving an admirable specific discharge capacity of 1.0}.5 mAh·g-1. 50 mA·g-1.an ultra-high capacity retention ratio of 97.97% after cycling 5000 times at 1.00 mA·g-1.nd a 96.1.%high capacity retention ratio after cycling 40,000 times at 5000 mA·g-1.***,with the support of ex situ TEM and XPS,the structural stability principle and anionic hybrid storage mechanism of CQD@CF electrode are investigated *** the full-cell system,HPDICs with the CQD@CF as cathode and nanographite powder as anode also present a 1.1.5 Wh·kg-1.igh energy density,a 5850 W·kg-1.ower density and a super-long cycle stability (90.2. capacity retention ratio after cycling 30,000 times at 5000 mA·g-1..

关键词： Anion hybrid storage mechanism Carbon quantum dots Potassium dual ion capacitor Ultrafast rechargeable Ultrathin carbon film

The ERF transcription factor LTF1.activates DIR1.to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

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Acta Pharmaceutica Sinica B 2024年第1期14卷 405-420页

作者： Ruibing chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng chen Shouhong Gao Xianghui chen qing Li Henan Zhang Wansheng chen Lei Zhang Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Shanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of Medicine Shanghai UniversityShanghai 200433China institute of Edible Fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life sciences and Medicine Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di Herbs Beijing 100700China

Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human *** the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant ***,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1.2.PLR,and ***1.was further identified as the key gene in respoJanuary 2.2.nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan ***,the phytohormone-responsive ERF transcription factor LTF1.colocalized with DIR1.in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of *** systematic results suggest that DIR1.as the first common step of the lignan pathway cooperates with PLR and UGT71.2.to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in *** roots and is also a part of the LTF1.mediated regulatory network to resist *** conclusion,the LTF1.DIR1.module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.

关键词： Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF

Human μ-opioid receptor overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins

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Cell Research 2000年第2期10卷 93-102页

作者： WEI QIANG DE HE ZHOU qing XIANG SHEN JIE {1. LI WEI {1. TIE LIN WANG GANG PEI ZHI QIANG CHI(e-mail:dhzhou@***)(1.Shanghai institute of Materia Medica,2.Shanghai institute of Cell biology,Shanghai academy of Life sciences, chinese Acad Shanghai institute of Materia Medica Shanghai Academy of Life Sciences Chinese Academy of Sciences Shanghai China Shanghai institute of Cell {1. Shanghai Academy of Life Sciences Chinese Academy of Sciences Shanghai China

Human μ-opioid receptor （HμOR） with a tag of six consecutive histidines at its carboxyl terminus had been expressed in recombinant baculovirus infected Sf9 insect *** maximal binding capacity for the [3H] diprenorphine and [3H]ohmefentanyl （Ohm） were 9.1. 0.7 and 6.52.0.2. nmol/g protein, respectively. The [3H] diprenorphine or [3H] Ohm binding to the receptor expressed in Sf9 cells was strongly inhibited by μ-selective agonists [D-Ala2., N-methylPhe4, glyol5]enkephalin （DAGO）, Ohm, and morphine, but neither by δ nor by K selective agonist. Na+（1.0 mM） and GTP （50 μM） could reduce HμOR agonists etorphine and Ohm affinity binding to the overexpressed HμOR. μ-selective agonists DAGO and Ohm effectively stimulated [35S]GTPγS binding (EC50= 2.7nM and 6.9 nM) and inhibited forskolin- stimulated cAMP accumulation (IC50= 0.9 nM and 0.3 nM). The agonist-dependent effects could be blocked by opioid antagonist naloxone or by pretreatment of cells with pertussis toxin （PTX）. These results demonstrated that HμOR overexpressed in Sf9 insect cells functionally coupled to endogenous Gi/o proteins.

关键词： Human μ-opioid receptor (HμOR) Sf9 insect cells pertussis toxin (PTX) endogenous Gi/o proteins

Two aquaporins,PIP1.1.and PIP2.1.mediate the uptake of neonicotinoid pesticides in plants

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Plant Communications 2024年第5期5卷 176-188页

作者： Qun Wan Yixin Li Jinjin cheng Ya Wang Jing Ge Tingli {1. Liya Ma Yong Li Jianan {1. Chunli Zhou Haocong Li Xing Sun Xiaolong chen {2. X.Li Xiangyang Yu Key Laboratory of Food Quality and Safety of Jiangsu Province State Key Laboratory Breeding BaseNanjing 210014China institute of Food Safety and Nutrition Jiangsu Academy of Agricultural Sciences50 Zhongling StreetNanjing 210014China Jiangsu Provincial Key Construction Laboratory of Special Biomass Resource Utilization Nanjing Xiaozhuang University3601 Hongjin AvenueNanjing 211171China Department of Molecular Biosciences and Bioengineering University of Hawaii at ManoaHonoluluHI 96822USA

Neonicotinoids(NEOs),a large class of organic compounds,are a type of commonly used pesticide for crop *** uptake and accumulation in plants are prerequisites for their intra-and intercellular move-ments,transformation,and *** the molecular mechanisms that underpin NEO uptake by plants is crucial for effective application,which remains ***,we demonstrate that NEOs enter plant cells primarily through the transmembrane symplastic pathway and accumulate mainly in the *** plasma membrane intrinsic proteins discovered in Brassica rapa,BraPIP1.1.and BraPIP2.1.were found to encode aquaporins(AQPs)that are highly permeable to NEOs in different plant species and facilitate NEO subcellular diffusion and *** conserved transport function was further demonstrated in Xenopus laevis oocyte and yeast ***1.1.and BraPIP2.1.gene knockouts and interaction as-says suggested that their proteins can form functional *** of the potential of mean force indicated a negative correlation between NEO uptake and the energy barrier of BraPIP1.1.*** study shows that AQPs transport organic compounds with greater osmolarity than previously thought,providing new insight into the molecular mechanisms of organic compound uptake and facilitating innovations in systemic pesticides.

关键词： neonicotinoid aquaporin Brassica rapa plasma membrane uptake systemic pesticide

50-1.0 GHz,high isolation,and high-power linearity single-pole double-throw switch utilizing 1.0-nm GaN HEMT technology

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The Journal of China Universities of Posts and Telecommunications 2024年第2期31卷 38-43,71页

作者： Han Qunfei Hu Sanming Zhang Tianyu chen {1. Shen Yizhu Wang Weibo Tao Hongqi School of Information Science and Engineering Southeast UniversityNanjing 210096China Nanjing Electronic Devices institute Nanjing 210016China State Key Laboratory of Millimeter Waves Southeast UniversityNanjing 210096China

This article presents the design and performance of a single-pole double-throw(SPDT)switch operating in 50-1.0 *** switch is fabricated in a 1.0-nm GaN high-electron-mobility transistors(HEMT)*** realize high-power capability,the dimensions of GaN HEMTs are selected by simulation *** enhance the isolation,an improved structure of shunt HEMT with two ground holes is *** extend the operation bandwidth,the SPDT switch with multi-section resonant units is proposed and *** verify the SPDT switch design,a prototype operating in 50-1.0 GHz is *** measured results show that the fabricated SPDT switch monolithic microwave integrated circuit(MMIC)achieves an input 1.dB compression point(P_(1.B))of 38 dBm at 94 GHz,and isolation within the range of 33 dB to 54 dB in 50-1.0 *** insertion loss of the switch is less than 2.1.dB,while the voltage standing wave ratios(VSWR)of the input port and output port are both less than 1.8 in the operation *** on the measured results,the presented SPDT switch MMIC demonstrates high power capability and high isolation compared with other reported millimeter-wave SPDT MMIC designs.

关键词： GaN high-electron-mobility transistors(HEMT) millimeter-wave single-pole double-throw(SPDT) switch

Influence of Ru addition on microstructure and stress-rupture property of Ni-based single crystal superalloys

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Progress in Natural Science:Materials International 2010年第1期20卷 61-69页

作者： Jing-yang chen~1.Qiang FENG~(1.{1.,La-mei CAO~3,Zu-qing SUN~1.1.State Key Laboratory for Advanced Metals and Materials,University of Science and Technology Beijing, Beijing 1.0083,China {1.National Center for Materials Service Safety,University of Science and Technology Beijing,Beijing 1.0083,China 3.National Key Laboratory of Science and Technology on Advanced High Temperature Structural Materials, Beijing {{1. of Aeronautical Materials,Beijing 1.0095,China State Key Laboratory for Advanced Metals and Materials University of Science and Technology Beijing Beijing 100083 China National Center for Materials Service Safety University of Science and Technology Beijing Beijing 100083 China National Key Laboratory of Science and Technology on Advanced High Temperature Structural Materials Beijing Institute of Aeronautical Materials Beijing 100095 China

The effects of Ru on the microstructure and stress-rupture property were investigated in two experimental Ni-based single crystal superalloys with and without Ru addition(5.1.,mass fraction).The results indicate that the morphology ofγ' precipitates in the dendrite core and interdendritic region are intermediate and nearly spherical,respectively,in the Ru-free alloy after being aged at 1.00℃for 8 *** Re partitioning ratio increases due to Ru addition and Ru partitioned preferentially to theγphase in both dendrite core and interdendritic region of the Ru-containing alloy,which results in more negative lattice misfit and more cuboidalγ' ***,theγ' volume fraction increases while the precipitate size and y channel width decreases due to Ru addition in both dendrite core and interdendritic *** addition improves the stress-rupture life at 1.00℃/1.0 MPa significantly due to higherγ' volume fraction,more negative lattice misfit and a well rafting structure as well as lessγchannel width.

关键词： Ni-based superalloys ruthenium elemental partitioning ratio lattice misfit γ’ morphology stress-rupture property

Spatial Characteristics of Soil Organic Carbon Storage in China's Croplands

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Pedosphere 2005年第4期15卷 417-423页

作者： WANG Shao-Qiang YU Gui-Rui ZHAO Qian-Jun NIU Dong chen {1.-Mei WU Zhi-Feng institute of Geographic {1. and Natural Resources Research Beijing 10010 （China）. Guangdong institute of Eco-Environment and Soil Science Guangzhou 510650 （China）

The soil organic carbon (SOC) pool is the largest component of terrestrial carbon pools. With the construction of a geographically referenced database taken from the second national general soil survey materials and based on 1.46 typical cropland soil profiles, the paddy field and dryland SOC storage among six regions of China were systematically quantified to characterize the spatial pattern of cropland SOC storage in China and to examine the relationship between mean annual temperature, precipitation, soil texture features and SOC content. In all regions, paddy soils had higher SOC storage than dryland soils, and cropland SOC content was the highest in Southwest China. Climate controlled the spatial distribution of SOC in both paddy and dryland soils, with SOC storage increasing with increasing precipitation and decreasing with increasing temperature.

关键词： carbon storage cropland soil organic carbon spatial characteristics

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仿驾驶员多目标决策自适应巡航鲁棒控制

控制理论与应用 2018年第6期35卷 769-777页

作者：章军辉李庆陈大鹏中国科学院微电子研究所北京100029 中国科学院大学北京100049 中科院微电子研究所昆山分所江苏昆山215347

针对目前市场上自适应巡航(adaptive cruise control,ACC)用户使用率低与驾乘人员接受度差的问题,发展了一种多目标自适应巡航控制算法,基于模型预测控制(model predictive control,MPC)理论,综合协调动态追踪性、燃油经济性、驾乘舒适... 详细信息

针对目前市场上自适应巡航(adaptive cruise control,ACC)用户使用率低与驾乘人员接受度差的问题,发展了一种多目标自适应巡航控制算法,基于模型预测控制(model predictive control,MPC)理论,综合协调动态追踪性、燃油经济性、驾乘舒适性以及跟车安全性四项存在着一定冲突的控制目标,采用二次型性能指标以及线性不等式约束的形式,将纵向期望加速度决策问题转化成带约束的在线二次规划问题.引入误差修正项,建立闭环反馈校正机制,以补偿模型失配带来的预测误差,同时采用松弛向量法扩展求解可行域,规避了硬约束下二次规划非可行解问题.进一步,各工况下所强化的性能指标与约束空间不同,采用性能指标权重微校与约束空间边界松弛的策略,设计出3种工作模式ACC,以适应熟练驾驶群体的跟驰习惯.仿真结果表明,前车组合工况下,多模式多目标ACC控制能够实现各工作模式之间的无缝切换,并实现良好的期望跟车目的,从而增强ACC系统对复杂道路交通环境的适应性.

关键词：半自动驾驶自适应巡航控制模型预测控制松弛向量跟驰习惯