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Changes in bacterial community and abundance of functional genes in paddy soil with cry1Ab transgenic rice

Journal of Integrative Agriculture 2021年第6期20卷 1674-1686页

作者： SONG Ya-na chen Zai-jie WU Ming-ji li Gang wang Feng Institute of Biotechnology Fujian Academy of Agricultural Sciences/Fujian Provincial Key Laboratory of Genetic Engineering for AgricultureFuzhou 350003P.R.China

A field experiment involving cry1Ab transgenic rice(GM) and its parental non-cry1Ab rice(M) has been on-going since 2014. The diversity of the bacterial communities and the abundance of the microbial functional genes which drive the conversion of nitrogen in paddy soil were analyzed during the growth period of rice in the fifth year of the experiment, using 16 S rRNAbased Illumina Mi Seq and real-time PCR on the amoA, nirS and nirK genes. The results showed no differences in the alpha diversity indexes of the bacterial communities, including Chao1, Shannon and Simpson, between the fields cultivated with line GM and cultivar M at any of the growth stages of rice. However, the bacterial communities in the paddy soil with line GM were separated from those of paddy soil with cultivar M at each of the growth stages of rice, based on the unweighted Uni Frac NMDS or PCoA. In addition, the analyses of ADONIS and ANOSIM, based on the unweighted Uni Frac distance, indicated that the above separations between line GM and cultivar M were statistically significant(Pline GM or cultivar M, respectively, led to the differences in the bacterial communities between them. At the same time, functional gene prediction based on Illumina Mi Seq data suggested that the abundance of many functional genes increased in the paddy soil with line GM at the maturity stage of rice, such as genes related to the metabolism of starch, amino acids and nitrogen. Otherwise, the copies of bacterial amo A gene, archaeal amo A gene and denitrifying bacterial nir K gene significantly increased(P1) in the paddy soil with line GM. In summary, the release of cry1Ab transgenic rice had effects on either the composition of bacterial communities or the abundance of microbial functional genes in the paddy soil.

关键词： cry1Ab transgenic rice bacterial community microbial functional gene Illumina MiSeq Platform real-time PCR

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Comparative risk of acute kidney injury among cancer patients treated with immune checkpoint inhibitors

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Cancer Communications 2023年第2期43卷 214-224页

作者： Fei liu Zixianwang Xiaofan li Zhen Zhang Yue Yang Junquan chen Dinghua chen lingling Wu Xiangyu liu Sujun Han Fangming wang Wasilijiang Wahafu Yibo gao Shancheng Ren Nianzeng Xing Guangyan Cai Xiangmei chen Department of Urology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Laboratory of Translational Medicine National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Thoracic Surgery National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Department of Medical Oncology Sun Yat-sen University Cancer CenterGuangzhouGuangdongP.R.China Department of Nephrology First Medical Center of Chinese PLA General HospitalNephrology Institute of the Chinese People’s Liberation ArmyState Key Laboratory of Kidney DiseasesNational Clinical Research Center for Kidney DiseasesBeijing Key Laboratory of Kidney Disease ResearchBeijingP.R.China Department of Plastic Surgery Plastic Surgery HospitalChinese Academy of Medical SciencesPeking Union Medical CollegeBeijingPR China State Key Laboratory of Molecular Oncology National Cancer Center/National Clinical Research Center for Cancer/Cancer HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijingP.R.China Central Laboratory National Cancer Center/National Clinical Research Center for Cancer/Cancer Hospital&Shenzhen HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeShenzhenGuangdongP.R.China Department of Urology Shanghai Changzheng HospitalShanghaiP.R.China

With the development and introduction of immune checkpoint inhibitors(ICIs)in cancer patients,immune-related side effects have increasingly attracted ***,the risks of immune-related renal toxicity are poorly *** this study,we performed a network meta-analysis(NMA)of ICI-related randomized clinical trials(RCTs)to elucidate the comparative risk of acute kidney injury(AKI)in cancer patients receiving different *** also sought to identify other factors potentially affecting the risk of *** and EMBASE were searched for peer-reviewed trial reports published between January 2000 and May *** studies were RCTs studying ICIs in cancer patients and reporting AKI *** performed a frequentist NMA to evaluate the risk ratios for grade 1-5 and grade 3-5 AKI between the treatment *** also assessed the absolute incidence of AKI in the ICI-containing arm using traditional direct *** significant heterogeneity was detected in a traditional direct meta-analysis,multivariable meta-regression analysis was applied to identify factors that significantly affected the absolute incidence of AKI.A total of 85 RCTs were included in this *** the NMA for the risk of grade 1-5 and 3-5 AKI,ipilimumab showed a significantly higher risk than avelumab and durvalumab,whereas 1 mg/kg nivolumab plus 3 mg/kg ipilimumab(N1I3)showed a significantly higher risk than other *** terms of treatment ranking,durvalumab±low-dose tremelimumab and avelumab were consistently among the top three safest treatments for grade 1-5 or 3-5 AKI,whereas N1I3,ipilimumab and tremelimumab were consistently among the top three treatments with the highest risk for grade 1-5 or 3-5 *** with other cancers,renal cell carcinoma and urothelial carcinoma showed a significantly higher risk of *** incidence of AKI was significantly higher with ICI+chemotherapy than with ICI *** this NMA involving largescale up-to-date ICI trials,we dem

关键词： immune checkpoint inhibitors acute kidney injury cancer immune therapy side effects

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高效液相色谱手性固定相研究进展

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山东轻工业学院学报（自然科学版） 2004年第1期18卷 69-73页

作者：寿崇琦张志良赵春宾邢希学李关宾陈立仁中国科学院兰州化学物理研究所甘肃兰州730000 济南大学化学化工学院山东济南250022

对近年来高效液相色谱手性固定相的研究进行了综述。重点介绍了手性固定相的分类、拆分机理和应用的新进展。讨论了各类手性固定相优缺点,提出了目前存在的问题、今后的研究方向和重点。

关键词：高效液相色谱法手性固定相分类拆分机理手性分离

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利用55K SNP芯片研究小麦新品种信麦163的遗传构成

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山东农业科学 2024年第5期56卷 42-48页

作者：陈真真李杰王轲陈金平申冠宇谢旭东石守设杨军周国勤信阳市农业科学院河南信阳464000 信阳鸿润家庭农场有限公司河南信阳464000

为明确国审小麦新品种信麦163的分子遗传基础,利用小麦55K SNP育种芯片对信麦163及其母本信阳234和父本丰抗38进行分析。结果表明,信阳234和丰抗38对信麦163的遗传贡献率分别为49.60%和50.40%。在基因组和染色体水平上,双亲对信麦163的... 详细信息

为明确国审小麦新品种信麦163的分子遗传基础,利用小麦55K SNP育种芯片对信麦163及其母本信阳234和父本丰抗38进行分析。结果表明,信阳234和丰抗38对信麦163的遗传贡献率分别为49.60%和50.40%。在基因组和染色体水平上,双亲对信麦163的遗传贡献率差异较大:母本信阳234对信麦163 A、B、D三个基因组的贡献率分别为49.34%、52.52%和45.61%,贡献率超过50%的染色体有4A、5A、7A、4B、5B、6B、7B、1D、5D、6D和7D,其中在7A、7B、7D上遗传贡献率超过60%;父本丰抗38对信麦163 A、B、D三个基因组的贡献率分别为50.66%、47.48%和54.39%,贡献率超过50%的染色体有1A、2A、3A、6A、1B、2B、3B、2D、3D和4D,其中在4D染色体上遗传贡献率超过80%。在3A、4D、6B等染色体上的遗传形式主要表现为亲本遗传信息以染色体大片段形式传递到子代。SNP(单核苷酸多态性)基因型图谱、SNP位点分析与遗传贡献率分析结果具有较好的一致性。本研究结果展示了杂交育种对后代基因组造成的影响,可为信麦163在遗传改良和生产中的应用提供科学依据。

关键词：信麦163 遗传贡献 55K育种芯片单核苷酸多态性(SNP)

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长期“化肥有机替代”提升红壤腐解微食物网生态网络复杂性和稳定性

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土壤通报 2024年第2期55卷 448-459页

作者：罗美玲邢稳韩硕袁梦李忠芳张会民李冬初娄翼来中国农业科学院农业环境与可持续发展研究所北京100081 贺州学院食品与生物工程学院广西贺州542800 中国农业科学院农业资源与农业区划研究所北京100081

[目的]通过探究长期“化肥有机替代”对土壤腐解微食物网复杂性和稳定性的影响,为土壤可持续培肥管理提供理论依据。[方法]依托南方红壤长期施肥田间定位试验,利用高通量测序技术研究土壤腐解微食物网生物群落组成和多样性对长期化肥有... 详细信息

[目的]通过探究长期“化肥有机替代”对土壤腐解微食物网复杂性和稳定性的影响,为土壤可持续培肥管理提供理论依据。[方法]依托南方红壤长期施肥田间定位试验,利用高通量测序技术研究土壤腐解微食物网生物群落组成和多样性对长期化肥有机替代的响应,并通过共现网络分析方法评价长期化肥有机替代对土壤腐解微食物网生态网络复杂性和稳定性的影响。[结果]长期化肥有机替代显著改变了土壤细菌、真菌、原生动物及整体微食物网的物种群落组成,并显著提高土壤细菌、真菌、原生动物、线虫及整体微食物网生物群落的物种丰富度。各功能群生态网络的平均度、平均聚类系数、连通性和模块化程度等绝大多数拓扑参数均表现为化肥有机替代处理下较高;与常规施肥处理相比,化肥有机替代处理各功能群生态网络的鲁棒性较高,细菌、原生动物、线虫和整体微食物网生态网络的脆弱性较低。[结论]长期化肥有机替代提高了南方红壤细菌、真菌、原生动物、线虫及整体微食物网生物群落的物种多样性,并提升了微食物网的生态网络复杂性和稳定性,对土壤生态功能有着潜在的改善作用。

关键词：化肥有机替代土壤微食物网线虫原生动物微生物生态网络

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QTL analysis across multiple environments reveals promising chromosome regions associated with yield-related traits in maize under drought conditions

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The Crop Journal 2021年第4期9卷 759-766页

作者： Xinmin Hu Guihua wang Xuemei Du Hongwei Zhang Zhenxiang Xu Jie wang Guo chen Bo wang Xuhui li Xunji chen Junjie Fu Jun Zheng Jianhua wang Riliang Gu Guoying wang beijing Innovation Center for Crop Seed Technology and beijing Key Laboratory of Crop Genetic Improvement Ministry of Agriculture and Rural AffairsKey Laboratory of Crop Heterosis UtilizationMinistry of EducationCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China Institute of Nuclear Technology and Biotechnology Xinjiang Academy of Agricultural SciencesUrumqi 830091XinjiangChina

Drought is one of the most critical abiotic stresses influencing maize yield. Improving maize cultivars with drought tolerance using marker-assisted selection requires a better understanding of its genetic basis. In this study, a doubled haploid(DH) population consisting of 217 lines was created by crossing the inbred lines Han 21(drought-tolerant) and Ye 478(drought-sensitive). The population was genotyped with a 6 K SNP assay and 756 SNP(single nucleotide polymorphism) markers were used to construct a linkage map with a length of 1344 c M. Grain yield(GY), ear setting percentage(ESP), and anthesis–silking interval(ASI) were recorded in seven environments under well-watered(WW) and water-stressed(WS) regimes. High phenotypic variation was observed for all traits under both water regimes. Using the LSMEAN(least-squares mean) values from all environments for each trait, 18 QTL were detected, with 9 associated with the WW and 9 with the WS regime. Four chromosome regions,Chr. 3: 219.8–223.7 Mb, Chr. 5: 191.5–194.7 Mb, Chr. 7: 132.2–135.6 Mb, and Chr. 10: 88.2–89.4 Mb, harbored at least 2 QTL in each region, and QTL co-located in a region inherited favorable alleles from the same parent. A set of 64 drought-tolerant BC_3F_6 lines showed preferential accumulation of the favorable alleles in these four regions, supporting an association between the four regions and maize drought tolerance. QTL-by-environment interaction analysis revealed 28 ed QTL(environment-dependent QTL) associated with the WS regime and 22 associated with the WW regime for GY, ESP, and ASI. All WS QTL and55.6% of WW QTL were located in the ed QTL regions. The hotspot genomic regions identified in this work will support further fine mapping and marker-assisted breeding of drought-tolerant maize.

关键词： Drought Yield Quantitative trait locus Introgression Maize

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Experimental test of robustness of composite pulses to non-static classic noise in NMR

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Science china(Physics,Mechanics & Astronomy) 2017年第2期60卷 80-81页

作者： li Xuechen ZHANG PanPan chu JingDi li JiYuan JIA PengYing Key Lab of Photo-Electronics Information Materials of Hebei Provinc

High fidelity and robust quantum control is the requirement for Quantum Information Processing （QIP） and quantum cornputing applications. Many negative factors, like pulse length error, system-bath coupling and the fluctuation of electronic instruments, deviate the quantum system evolution from the ideal path. To suppress these errors, many quantum manipulation techniques have been proposed. Composite pulses are designed to suppress off resonance error, pulse length error or the combination of them.

关键词：噪声鲁棒性脉冲核磁共振复合量子信息处理实验静态长度误差

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内感受损伤对药物成瘾的影响及其机制研究进展

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科学通报 2024年第7期69卷 891-902页

作者：谢倩倩周新雨文雅童李勇辉陈葸静中国科学院心理研究所中国科学院心理健康重点实验室北京100101 中国科学院大学心理系北京100049

内感受,即人对身体内部信号的感知、整合解释和调节能力,在药物成瘾的形成、发展和治疗过程中起到重要作用.长期的药物使用导致成瘾者无法准确地识别管理来自身体内部的信号,并对与药物效应相关的身体信号过分敏感,其背后的神经基础是... 详细信息

内感受,即人对身体内部信号的感知、整合解释和调节能力,在药物成瘾的形成、发展和治疗过程中起到重要作用.长期的药物使用导致成瘾者无法准确地识别管理来自身体内部的信号,并对与药物效应相关的身体信号过分敏感,其背后的神经基础是以岛叶为核心的内感受处理中枢的损伤.内感受的功能障碍可能导致个体对药物的过度依赖和无法自控.受损的内感受通过影响线索诱发的药物渴求、增加负性情绪和冲动、损伤风险决策能力导致成瘾者的复吸.本文探究内感受的损伤特征及其在药物成瘾过程中的作用,有助于识别药物成瘾形成的诱因、评估成瘾严重程度,为干预提供一个外周、中枢、行为和主观报告相结合的多维度靶点.未来的研究可从多模态评估内感受能力、建立内感受神经和心理表征的对应关系、探究不同药物的内感受损伤特征共性和特异性等方向进一步明确内感受在药物成瘾中的作用,并探索内感受作为跨诊断和共病干预的靶点的可行性.

关键词：药物成瘾内感受复吸渴求

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Identification of methylated differentially expressed hub genes in systemic lupus erythematosus patients by WGCNA-based epigenome-wide and transcriptome-wide analysis

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Genes & Diseases 2023年第5期10卷 1771-1774页

作者： Qiao Zhou li Long Xixi chen Xiaobo Huang Shaoping Deng Yi wang Department of Rheumatology and Immunology Sichuan Academy of Medical Science and Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Clinical Immunology Translational Medicine Key Laboratory of Sichuan Province Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China Department of Critical Care Medicine Sichuan Academy of Medical Science and Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengduSichuan 610072China

Systemic lupus erythematosus(SLE)is an autoimmune disease,characterized by the production of autoantibodies and the involvement of *** order to explore its molecular mechanism,we,using bioinformatics analysis and wet ... 详细信息

关键词： lupus erythematosus SLE

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金针菇冰结合蛋白的研究进展

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黑龙江科学 2012年第2期3卷 34-37页

作者：陈静宇王佳龙陈丽娟杨志兴黑龙江省科学院微生物研究所黑龙江哈尔滨150010 黑龙江省科学院高技术研究院黑龙江哈尔滨150090

论述了金针菇等蘑菇的冰结合蛋白的提取、纯化、活性测定、序列分析及其基因的研究。同时简要介绍了抗冻蛋白和冰结合蛋白及其基因在实践中的应用进展。

关键词：金针菇蘑菇硅藻冰结合蛋白基因

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