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Grass phasiRNAs and male fertility

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Science China(Life Sciences) 2018年第2期61卷 148-154页

作者： Yang Yu Yanfei Zhou Yuchan Zhang yueqin chen Guangdong Provincial Key Laboratory of Plant Resources State Key Laboratory for Biocontrol School of Life Sciences Sun Yat-sen University

Recent studies have indicated that a special type of small noncoding RNAs, phased small-interfering RNAs(phasiRNAs) play crucial roles in many cellular processes of plant development. PhasiRNAs are generated from long RNA precursors at intervals of 21 or 24 nt in plants, and they are produced from both protein-coding gene and long noncoding RNA genes. Different from those in eudicots, grass phasiRNAs include a special class of small RNAs that are specifically expressed in reproductive *** grass phasiRNAs are associated with gametogenesis, especially with anther development and male fertility. In this review,we summarized current knowledge on these small noncoding RNAs in male germ cells and their possible biological functions and mechanisms in grass species.

关键词： noncoding RNA phasiRNA crop fertility

Identification and evolution-ary implication of four novel box H/ACA snoRNAs from Giardia lamblia

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Chinese Science Bulletin 2006年第20期51卷 2451-2456页

作者： LUO Jun ZHOU Hui chen Chongjian LI Yan chen yueqin QU Lianghu Key Laboratory of Gene Engineering of the Ministry of Education StateKey Laboratory for Biocontrol Zhongshan University Guangzhou510275 China

From a specialized cDNA library, four novel box H/ACA snoRNAs, named GLsR22, GLsR23, GLsR24 and GLsR25, were identified from the primitive eukaryote, Giardia lamblia. Bioinformat- ics analyses indicated that all of them can be poten- tially folded into double hairpins, the typical secon- dary structures of box H/ACA snoRNAs. GLsR24 and GLsR25 are predicted to guide the site-specific pseudouridylation at U1753 and U2396 on 23S rRNA, respectively, while GLsR22 and GLsR23 belong to the family of “orphan” snoRNAs. All of the four novel snoRNAs are encoded by single copy genes and located in small intergenic regions. Interestingly, compared with the counterparts previously reported in Archaea and other unicellular protozoan, the box H/ACA snoRNAs identified from G. lamblia have unique structural features, implying that snoRNAs evolved from prokaryotes to eukaryotes in different ways.

关键词：贾第虫兰氏鞭毛虫基因工程核糖核酸

The m6A writers regulated by the IL-6/STAT3 inflammatory pathway facilitate cancer cell stemness in cholangiocarcinoma

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Cancer Biology & Medicine 2022年第3期19卷 343-357页

作者： Hua Ye Tianqi chen Zhancheng Zeng Bo He Qianqian Yang Qi Pan yueqin chen Wentao Wang Department of Hepatobiliary Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China Key Laboratory of Gene Engineering of the Ministry of Education School of Life SciencesSun Yat-sen UniversityGuangzhou 510275China Department of Anesthesiology Sun Yat-sen Memorial HospitalSun Yat-sen UniversityGuangzhou 510120China

Objective:Investigation of the regulatory mechanisms of cell stemness in cholangiocarcinoma(CCA)is essential for developing effective therapies to improve patient *** purpose of this study was to investigate the function and regulatory mechanism of m6A modifications in CCA cell ***:Interleukin 6(IL-6)treatment was used to induce an inflammatory response,and loss-of-function studies were conducted using mammosphere culture *** immunoprecipitation,polysome profiling,and methylated RNA immunoprecipitation analyses were used to identify signaling *** in vitro findings were verified in a mice ***:We first identified that m6A writers were highly expressed in CCAs and further showed that STAT3 directly bound to the gene loci of m6A writers,showing that IL-6/STAT3 signaling regulated expressions of m6A *** m6A writers prevented cell proliferation and migration in vitro and suppressed CCA tumorigenesis in ***,the knockdown of m6A writers inhibited CCA cell stemness that was triggered by IL-6 ***,IGF2BP2 was bound to CTNNB1 transcripts,significantly enhancing their stability and translation,and conferring stem-like ***,we confirmed that the combination of m6A writers,IGF2BP2,and CTNNB1 distinguished CCA tissues from normal ***:Overall,this study showed that the IL-6-triggered inflammatory response facilitated the expressions of m6A writers and cell stemness in an m6A-IGF2BP2-dependent ***,the study showed that m6A modification was a targetable mediator of the response to inflammation factor exposure,was a potential diagnostic biomarker for CCA,and was critical to the progression of CCA.

关键词： Cholangiocarcinoma IL-6 cell stemness N6-methyladenosine(m6A) IGF2BP2

Protective effects of ferulic acid against ionizing radiation-induced oxidative damage in rat lens through activating Nrf2 signal pathway

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International Journal of Ophthalmology(English edition) 2023年第5期16卷 687-693页

作者： yueqin chen Jiawei Shen Xiaoran Zhang Weiping Gao Qian Cao Feng Yan Chunyan Xue Department of Ophthalmology Nanjing Drum Tower HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210008Jiangsu ProvinceChina Nanjing University of Chinese Medicine Nanjing 210029Jiangsu ProvinceChina Department of Ophthalmology Nanjing Jinling HospitalAffiliated Hospital of Medical SchoolNanjing UniversityNanjing 210002Jiangsu ProvinceChina

AIM: To examine the protection of ferulic acid(FA) against ionizing radiation(IR)-induced lens injury in rats, as well as the underlying ***: FA(50 mg/kg) was administered to rats for 4 consecutive days before they were given 10 Gy γ-radiation, as well as for 3 consecutive days afterward. Two weeks after radiation, the eye tissues were collected. Histological alterations were evaluated by hematoxylineosin staining. Enzyme linked immunosorbent assay(ELISA) was utilized to assess the activities of glutathione reductase(GR) and superoxide dismutase(SOD), as well as the levels of glutathione(GSH) and malondialdehyde(MDA) in the lenses. The protein and m RNA levels of Bcl-2, caspase-3, Bax, heme oxygenase-1(HO-1), and glutamatecysteine ligase catalytic subunit(GCLC) were quantified using Western blot and quantitative reverse transcription polymerase chain reaction, respectively. With nuclear extracts, the nuclear factor erythroid-2 related factor(Nrf2) protein expressions in the nuclei were also ***: Rats exposed to IR showed lens histological alterations which could be alleviated by FA. FA treatment reversed apoptosis-related markers in IR-induced lens, as evidenced by lower levels of Bax and caspase-3 and higher level of Bcl-2. Furthermore, IR induced oxidative damage manifested by decreased GSH level, increased MDA level, and decreased SOD and GR activities. FA boosted nuclear translocation of Nrf2 and increased the expressions of HO-1 and GCLC to inhibit oxidative stress, as evidenced by an increase in GSH, a decrease in MDA, and an increase in GR and SOD ***: FA may work well in preventing and treating IR-induced cataract through promoting the Nrf2 signal pathway to attenuate oxidative damage and cell apoptosis.

关键词： ferulic acid ionizing radiation lens oxidative stress Nrf2

Oxidation kinetics regularity in spontaneous combustion of gas coal

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Mining Science and Technology 2010年第1期20卷 59-63页

作者： WANG Lanyun JIANG Shuguang WU Zhengyan SHAO Hao ZHANG Weiqing chen yueqin ZOU Lili State Key Laboratory of Coal Resources and Mine Safety China University of Mining & Technology Xuzhou 221008 China School of Safety Engineering China University of Mining & Technology Xuzhou 221008 China

In order to investigate the oxidation kinetics of gas coal at low temperatures, we derived a rate equation of oxygen consumption during low-temperature oxidation of gas coal and deduced an E-c equation, expressing the relation between active energy E and oxygen concentration c. The reaction order n and active energy E were calculated with this equation based on experiments of static oxygen consumption tests. In addition, we proved the rationality of the E-c equation using a kinetic compensation effect and obtained the isokinetic temperature Tc. The results show that： 1） the gas coal oxidizes easily with increasing temperature and the oxidation tends to be spontaneous at higher temperatures; 2） the oxygen concentration c affects oxygen consumption very much at lower temperatures but has only a small effect at higher temperatures; 3） the isokinetic temperature Tc was 127 ℃ which has been experimentally validated as the key turning point during low-temperature spontaneous combustion of gas coal.

关键词： static oxygen consumption test system reaction order active energy kinetic compensation effect isokinetic temperature

New insight into the role of miRNAs in leukemia

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Science China(Life Sciences) 2009年第3期52卷 224-231页

作者： ZHANG Hua & chen yueqin Key Laboratory of Genetic Engineering of the Ministry of Education,State Key Laboratory of Biocontrol,Sun Yan-Sen University,Guangzhou 510275,China 1. Key Laboratory of Genetic Engineering of the Ministry of Education State Key Laboratory of Biocontrol Sun Yan-Sen University Guangzhou 510275 China

Recent studies have shown that microRNAs(miRNAs) play an important role in cell differentiation,growth,and death,including the functional study of miRNAs in *** date,miRNA expression profiles in many types of cancers have been identified and miRNA expression signatures associated with types and cytogenetics of leukemia have also been *** evidence has shown that miRNAs could function as either tumor suppressors or oncogenes in cancers such as leukemia,while other miRNAs might be benefitcial for diagnosis and prognosis,predicted to be newly developed *** this review,we summarize the recent progress about miRNAs in leukemia and present a miRNA-mediated network involved in differentiation,proliferation and apoptosis predicted to be the roles of miRNAs in the pathogenesis of leukemia.

关键词： miRNA leukemia regulation network clinical implication

Identification and functional analysis of 23 novel box C/D snoRNAs from Oryza sativa

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Chinese Science Bulletin 2003年第19期48卷 2077-2082页

作者： ZHUO Min, ZHOU Hui, HUANG Zhanpeng, LIANG Dan, chen Chunlong, chen yueqin & QU Lianghu Key Laboratory of Gene Engineering of the Ministry of Education, Biotechnology Research Center, Zhongshan University, Guangzhou 510275, China Key Laboratory of Gene Engineering of the Ministry of Education Biotechnology Research Center Zhongshan University Guangzhou 510275 China Yarmouk University Irbid - Jordan

In a cDNA library generated from rice small nuclear RNAs, 30 box C/D small nucleolar RNAs (snoRNAs) were identified through preliminary screen. Except 7 known snoRNAs such as U14, all snoRNAs were identified in rice for the first time experimentally. Among the 23 novel snoRNAs, 11 snoRNAs appear rice-specific, 6 snoRNAs are unique to plants, the remaining 6 snoRNAs have their counterparts in both Arabidopsis and yeast or mammals according to the conserved antisense sequences that guide 2′-O-ribose methylation of rRNA. 17 of the 23 novel snoRNAs were predicted to guide 24 2′-O-ribose methylations at the specific sites of rice 5.8S, 18S, 25S rRNAs, among which 19 methylated sites were determined by primer extension at low dNTP concentrations. The remaining 6 snoRNAs devoid of rRNA antisense elements may represent novel snoRNA species in rice. The results show that constructing a cDNA library from small nuclear RNAs is an effective experimental approach for novel snoRNA identification. The novel snoRNAs are important in elucidating the genomic organization and expression of plant snoRNA genes and the mechanism through which 2’-O-ribose methylations took place in rRNAs.

关键词：功能分析 cDNA snoRNAs 水稻基因表达

Comparison and significance of specific and non-specific cellular immunity in patients with chronic hepatitis B caused by infection with genotypes B or C of hepatitis B virus

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Science China(Life Sciences) 2009年第8期52卷 719-723页

作者： GU XiBing,YANG XiaoJuan,WANG Dong,HUA Zhong,WU HangYuan,chen HaoKun,XU yueqin & LU ZhongHua Wuxi Hospital for Infectious Diseases,Wuxi 214005,China 1. Wuxi Hospital for Infectious Diseases Wuxi 214005 China

The present study was designed to investigate possible relationships between the genotypes of hepatitis B virus(HBV) and the HBV-specific cytotoxic T lymphocyte(CTL) *** genotypes,HBV specific CTL HBV DNA and other markers of HBV infection were determined in 138 patients with chronic hepatitis *** results showed that the patients infected with genotype C(n=62) had a significantly lower HBV-specific CTL response than those who were infected with HBV genotype B(P<0.01).HBV DNA titer was higher in patients infected with HBV genotype C than in those infected with HBV geno-type B(P<0.01).Both alanine aminotransferase(ALT) and total bilirubin(TBIL) were higher in HBV genotype C infected patients than in those infected with genotype B(P<0.01 and <0.05,respectively).These results suggest that compared with CHB patients infected with HBV genotype B,the higher HBV DNA level and more severe liver damages in the patients infected with genotype C of HBV may be associated with genotype C of the virus.

关键词： chronic hepatitis B hepatitis B virus(HBV) genotypes specific cytotoxic T lymphocyte

简单并不代表原始──关于生物早期演化的一种新观点

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中山大学学报（自然科学版） 1999年第1期38卷 128-129页

作者：屈良鹄陈月琴中山大学生物工程研究中心

１９７７年，Ｗｏｅｓｅ等［１］通过对细菌ｒＲＮＡ分析，提出了生物分类的三大界学说，即真核生物、古细菌和真细菌．Ｉｗａｂｅ等［２］对一种在生物三大界分岐之前就发生重复的ＡＴＰ酶基因分析，进一步推测古细菌与真核生物更为接... 详细信息

１９７７年，Ｗｏｅｓｅ等［１］通过对细菌ｒＲＮＡ分析，提出了生物分类的三大界学说，即真核生物、古细菌和真细菌．Ｉｗａｂｅ等［２］对一种在生物三大界分岐之前就发生重复的ＡＴＰ酶基因分析，进一步推测古细菌与真核生物更为接近（图１ａ）．从图１ａ中还可以看出...

关键词：原核生物真核生物早期演化生物演化