检索结果-南通市图书馆

A Microarray Based Genomic Hybridization Method for Identification of New Genes in Plants：Case Analyses of Arabidopsis and Oryza

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Plant Biology 2007年第6期49卷 915-926页

作者： Chuanzhu Fan Maria D. Vibranovski ying chen Manyuan long Department of Ecology and Evolution The University of Chicago Chicago IL 60637 USA

To systematically estimate the gene duplication events In closely related species, we have to use comparative genomlc approaches, either through genomlc sequence comparison or comparative genomlc hybridization （CGH）. Given the scarcity of complete genomlc sequences of plant species, in the present study we adopted an array based CGH to Investigate gene duplications In the genus Arabldopsls. Fragment genomlc DNA from four species, namely Arabidopsls thallana, A. lyrata subsp, lyrata, A. lyrata subsp, petraea, and A. halleri, was hybridized to Affymetrlx （Santa Clara, CA, USA） tiling arrays that are designed from the genomlc sequences of A. thallana. Pairwlse comparisons of signal intensity were made to infer the potential duplicated candidates along each phylogenetic branch. Ninety-four potential candidates of gene duplication along the genus were Identified. Among them, the majority （69 of 94） were A. thallana lineage specific. This result indicates that the array based CGH approach may be used to Identify candidates of duplication In other plant genera containing closely related species, such as Oryza, particularly for the AA genome species. We compared the degree of gene duplication through retrotransposon between O. satlva and A. thallana and found a strikingly higher number of chimera retroposed genes In rice. The higher rate of gene duplication through retroposltlon and other mechanisms may Indicate that the grass species Is able to adapt to more diverse environments.

关键词： Arabidopsis comparative genomic hybridization microarray new genes Oryza retroposition.

Effects of Arbuscular Mycorrhizal Fungi Communities on Soil Quality and the Growth of Cucumber Seedlings in a Greenhouse Soil of Continuously Planting Cucumber

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Pedosphere 2012年第1期22卷 79-87页

作者： LI Yan chen ying-long LI Min LIN Xian-Gui LIU Run-Jin Institute of Mycorrhizal Biotechnology Qingdao Agricultural UniversityQingdao 266109(China) State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of SciencesNanjing210008(China) School of Earth and Environment The University of Western AustraliaPerth 6009(Australia)

A pot experiment was performed to determine the effects of arbuscular mycorrhizM fungi （AMF） communities on soil properties and the growth of cucumber seedlings in a degraded soil that had been used for continuous cucumber monoculture in a greenhouse for 15 years. In the experiment, AMF communities （created by combining various AMF species that were found to be dominant in natural farm soil） were inoculated into the degraded soil, and then the soil was planted with cucumber. Inoculation with AMF communities did not affect soil pH but increased soil aggregate stability and decreased the concentrations of salt ions and electrical conductivity （EC） in the soil. Inoculation with AMF communities increased the numbers of culturable bacteria and actinomycetes but reduced the number of fungi. AMF communities increased plant growth, soluble sugar content, chlorophyll content, and root activity compared to non-mycorrhizal or a single AMF species treatments. Improvements of soil quality and plant growth were greatest with the following two communities： Glomus etunicatum ＋ G. mosseae ＋ Gigaspora margarita ＋ Acaulospora lacunosa and G. aggregatum ＋ G. etunicatum ＋ G. mosseae ＋ G. versiforme ＋ G. margarita ＋ A. lacunosa. The results suggested that certain AMF communities could substantially improve the quality of degraded soil.

关键词： enzyme activities microbial community protected cultivation rhizosphere soluble sugar

Analysis of the Cellular Localization of Herpes Simplex Virus 1 Immediate-early Protein ICP22

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2010年第3期25卷 158-167页

作者： Wei CUN Jie chen ying ZHANG long-ding LIU Qi-han LI Institute of Medical Biology Chinese Academy of Medical Sciences Peking Union Medical College Kunming 650118 China

Nuclear proteins often form punctiform structures, but the precise mechanism for this process is unknown. As a preliminary study, we investigated the aggregation of an HSV-1 immediate-early protein, infected-cell protein 22 （ICP22）, in the nucleus by observing the localization of ICP22-EGFP fusion protein Results showed that, in high-level expression conditions, ICP22-EGFP gradually concentrates in the nucleus, persists throughout the cell cycle without disaggregation even in the cell division phase, and is finally distributed to daughter cells. We subsequently constructed a mammalian cell expression system, which had tetracycline- dependent transcriptional regulators. Consequently, the location of ICP22-EGFP in the nucleus changed with distinct induction conditions. This suggests that the cellular location of ICP22 is also influenced by promoter regulation, in addition to its own structure. Our findings provide new clues for the investigation of transcriptional regulation of viral genes. In addition, the non-protease reporter system we constructed could be utilized to evaluate the role of intemal ribosome entry sites （IRES） on transcriptional regulation.

关键词： Herpes Simplex Virus 1 (HSV-1) ICP22 Transcriptional regulation Cellular localization Nuclear functional domain

在线全文

学校读者我要写书评

暂无评论

盐胁迫对水稻籽粒灌浆特性及产量形成的影响

作物学报 2024年第3期50卷 734-746页

作者：韦还和张翔朱旺耿孝宇马唯一左博源孟天瑶高平磊陈英龙许轲戴其根江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室/江苏省粮食作物现代产业技术协同创新中心/扬州大学水稻产业工程技术研究院/农业农村部盐碱土改良与利用(滨海盐碱地)重点实验室/国家耐盐碱水稻技术创新中心华东中心扬州大学江苏扬州225009 扬州大学教育部农业与农产品安全国际合作联合实验室/扬州大学农业科技发展研究院扬州大学225009

以江苏沿海滩涂主栽常规粳稻南粳9108和淮稻5号为试材,研究不同盐胁迫处理包括对照(CK,盐浓度0)、中盐(medium-salinity stress,MS,盐浓度0.15%)和高盐(high-salinity stress,HS,盐浓度0.3%)对水稻籽粒灌浆及产量形成生理特性的影响。... 详细信息

以江苏沿海滩涂主栽常规粳稻南粳9108和淮稻5号为试材,研究不同盐胁迫处理包括对照(CK,盐浓度0)、中盐(medium-salinity stress,MS,盐浓度0.15%)和高盐(high-salinity stress,HS,盐浓度0.3%)对水稻籽粒灌浆及产量形成生理特性的影响。结果表明:(1)与对照相比,中盐和高盐胁迫均显著降低水稻产量,降幅分别为26.3%和57.7%(两品种平均);盐胁迫处理下,穗数、每穗粒数、结实率和千粒重均显著下降。(2)盐胁迫显著降低穗长、每穗强势粒和弱势粒籽粒数、结实率和千粒重,其中强势粒结实率和千粒重的降幅均低于弱势粒。(3)盐胁迫下,水稻植株抽穗期和成熟期干物重以及抽穗期至成熟期干物质积累量显著下降,但收获指数明显增加。此外,抽穗后15 d和30 d盐胁迫处理的叶片净光合速率和SPAD值均显著低于对照。(4)盐胁迫降低籽粒最大灌浆速率和平均灌浆速率,但达最大灌浆速率时间和有效灌浆天数有所增加;盐胁迫提高了强势粒和弱势粒有效灌浆天数,但平均灌浆速率显著下降,其中强势粒灌浆量的降幅低于弱势粒。(5)盐胁迫下,籽粒腺苷二磷酸焦磷酸化酶(AGPase)、淀粉合成酶(SSS)、颗粒型淀粉合成酶(GBSS)和淀粉分支酶(SBE)活性显著下降,其中弱势粒的降幅高于强势粒。综上所述,盐胁迫下水稻强势粒和弱势粒灌浆天数有所增加,但籽粒灌浆速率及其淀粉合成关键酶活性显著下降,致使籽粒充实度、粒重和产量显著下降,其中盐胁迫对弱势粒抑制作用大于强势粒。

关键词：盐胁迫水稻籽粒灌浆产量

维普期刊数据库博看期刊

Decreased panicle N application alleviates the negative effects of shading on rice grain yield and grain quality

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第7期22卷 2041-2053页

作者： WEI Huan-he GE Jia-lin ZHANG Xu-bin ZHU Wang DENG Fei REN Wan-jun chen ying-long MENG Tian-yao DAI Qi-gen Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Research Institute of Rice Industrial Engineering Technology/College of Agriculture Yangzhou UniversityYangzhou 225009P.R.China Institutes of Agricultural Sciences and Technology Development/Joint International Research Laboratory of Agriculture and Agri-Product Safety Ministry of Education/College of AgricultureYangzhou UniversityYangzhou 225009P.R.China Crop Ecophysiology and Cultivation Key Laboratory of Sichuan Province/Key Laboratory of Crop Ecophysiology and Farming System in Southwest China Ministry of Agriculture and Rural Affairs/College of AgronomySichuan Agricultural UniversityChengdu 611130P.R.China

Light deficiency is a growing abiotic stress in rice ***,few studies focus on shading effects on grain yield and quality of rice in East *** is also essential to investigate proper nitrogen(N)application strategies that can effectively alleviate the negative impacts of light deficiency on grain yield and quality in rice.A two-year field experiment was conducted to explore the effects of shading(non-shading and shading from heading to maturity)and panicle N application(NDP,decreased panicle N rate;NMP,medium panicle N rate;NIP,increased panicle N rate)treatments on rice yield-and quality-related *** with non-shading,shading resulted in a 9.5-14.8%yield loss(P<0.05),mainly due to lower filled-grain percentage and grain *** and NIP had higher(P<0.05)grain yield than NDP under non-shading,and no significant difference was observed in rice grain yield among NDP,NMP,and NIP under *** with NMP and NIP,NDP achieved less yield loss under shading because of the increased filled-grain percentage and grain *** reduced leaf photosynthetic rate after heading,as well as shoot biomass weight at maturity,shoot biomass accumulation from heading to maturity,and nonstructural carbohydrate(NSC)content in the stem at maturity(P<0.05).The harvest index and NSC remobilization reserve of NDP were increased under *** decreased(P<0.05)percentages of brown rice,milled rice,head rice,and amylose content while increasing(P<0.05)chalky rice percentage,chalky area,chalky degree,and grain *** demonstrated a better milling quality under non-shading,while NDP demonstrated under *** exhibited both lower chalky rice percentage,chalky area,and chalky degree under non-shading and shading,compared with NMP and *** under shading decreased amylose content and breakdown but increased grain protein content and setback,contributing to similar overall palatability to *** results suggested severe g

关键词： rice grain yield grain quality shading panicle N application

江苏省滨海盐碱地籼粳杂交稻产量优势形成特征

在线全文

学校读者我要写书评

暂无评论

作物学报 2024年第5期50卷 1253-1270页

作者：耿孝宇张翔刘洋左博源朱旺马唯一汪璐璐孟天瑶高平磊陈英龙许轲戴其根韦还和江苏省作物遗传生理重点实验室/江苏省作物栽培生理重点实验室/江苏省粮食作物现代产业技术协同创新中心/农业农村部盐碱土改良与利用(滨海盐碱地)重点实验室/国家耐盐碱水稻技术创新中心华东中心/扬州大学水稻产业工程技术研究院/扬州大学江苏扬州225009 扬州大学教育部农业与农产品安全国际合作联合实验室/扬州大学农业科技发展研究院/扬州大学江苏扬州225009

旨在探明滨海盐碱地大田条件下籼粳杂交稻产量优势形成特征。2021—2022年,在江苏省滨海盐碱地择取中度盐碱地(medium saline-alkali soil,MS,土壤电导率平均2.7 dSm^(-1))和重度盐碱地(high saline-alkali soil,HS,土壤电导率平均7.2 d... 详细信息

旨在探明滨海盐碱地大田条件下籼粳杂交稻产量优势形成特征。2021—2022年,在江苏省滨海盐碱地择取中度盐碱地(medium saline-alkali soil,MS,土壤电导率平均2.7 dSm^(-1))和重度盐碱地(high saline-alkali soil,HS,土壤电导率平均7.2 dSm^(-1))两类典型田块,以籼粳杂交稻(甬优2640和甬优4949)、常规粳稻(南粳9108和淮稻5号)、杂交籼稻(丰优香占和Y两优372)3种类型水稻品种为试材,探究实地条件下盐胁迫对不同类型品种产量及其形成特征的影响。与中度盐碱地相比:(1)重度盐碱地下各类型品种的穗数、每穗粒数、结实率和千粒重均显著低于中度盐碱地。中度和重度盐碱地下,籼粳杂交稻的产量均显著高于常规粳稻和杂交籼稻,这主要是由于其较高的每穗粒数。(2)重度盐碱地下各类型品种关键生育期(拔节、抽穗和成熟期)的群体茎蘖数及茎蘖成穗率均显著下降。中度和重度盐碱地下,常规粳稻和杂交籼稻关键生育期的群体茎蘖数及茎蘖成穗率均高于籼粳杂交稻。(3)重度盐碱地下各类型品种关键生育期的干物重均显著下降。中度及重度盐碱地下,籼粳杂交稻抽穗期和成熟期干物重、拔节期—抽穗期和抽穗—成熟期作物生长速率均显著高于常规粳稻和杂交籼稻。重度盐碱地下各类型品种的收获指数呈上升趋势。籼粳杂交稻在重度盐碱地下的收获指数高于常规粳稻和杂交籼稻。(4)重度盐碱地下各类型品种关键生育期的叶面积指数、播种期—拔节期、拔节期—抽穗期和抽穗期—成熟期光合势均显著下降。中度和重度盐碱地下,籼粳杂交稻成熟期叶面积指数和抽穗期—成熟期光合势均显著高于常规粳稻和杂交籼稻,抽穗期—成熟期叶面积指数衰减率则呈相反趋势。(5)重度盐碱地下各类型品种株高、上三叶叶长和叶宽、叶片SPAD值和光合速率均显著下降。中度和重度盐碱地下,籼粳杂交稻上三叶叶长和叶宽、叶片SPAD值和光合速率均高于常规粳稻和杂交籼稻。本研究结果表明,滨海盐碱地实地条件下,盐胁迫显著影响水稻产量及其形成特征。与常规粳稻和杂交籼稻相比,籼粳杂交稻在滨海盐碱地仍具有较高的产量优势,这主要得益于其较高的每穗粒数;此外,较高的作物生长速率、较大的上三叶叶面积及其较强的叶片持绿性也是籼粳杂交稻在滨海盐碱地具有较好产量优势的重要特征。

关键词：滨海盐碱地水稻籼粳杂交稻产量形成

维普期刊数据库博看期刊

An open dataset of data lineage graphs for data governance research

在线全文

学校读者我要写书评

暂无评论

Visual Informatics 2024年第1期8卷 1-5页

作者： Yunpeng chen ying Zhao Xuanjing Li Jiang Zhang Jiang long Fangfang Zhou Central South University ChangshaHunanChina Huawei Cloud Computing Technology Co. Ltd.HangzhouZhejiangChina

Data have become valuable assets for *** governance aims to manage and reuse data assets,facilitating enterprise management and enabling product innovations.A data lineage graph(DLG)is an abstracted collection of data assets and their data lineages in data *** DLGs can provide rich data insights for data ***,the progress of data governance technologies is hindered by the shortage of available open datasets for *** paper introduces an open dataset of DLGs,including the DLG model,the dataset construction process,and applied *** real-world dataset is sourced from Huawei Cloud Computing Technology Company Limited,which contains 18 DLGs with three types of data assets and two types of *** the best of our knowledge,this dataset is the first open dataset of DLGs for data *** dataset can also support the development of other application areas,such as graph analytics and visualization.

关键词： Data asset Data governance Data lineage Graph Open dataset

Morpho-physiological traits contributing to better yield performance of japonica/indica hybrids over indica hybrids under input-reduced practices

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2020年第11期19卷 2643-2655页

作者： WEI Huan-he MENG Tian-yao GE Jia-lin ZHANG Xu-bin LU Yu LI Xin-yue TAO Yuan DING Enhao chen ying-long DAI Qi-gen Jiangsu Key Laboratory of Crop Genetics and Physiology/Jiangsu Key Laboratory of Crop Cultivation and Physiology/Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Research Institute of Rice Industrial Engineering Technology Yangzhou UniversityYangzhou 225009P.R.China Institute of Agricultural Science and Technology Development Yangzhou University/Joint International Research Laboratory of Agriculture and Agri-Product SafetyMinistry of EducationYangzhou 225009P.R.China

It is widely reported that japonica/indica hybrids(JIH)have superior grain yield over other main varietal groups such as indica hybrids(IH)under sufficient resource *** date,little attention has been paid to yield performance of JIH under input-reduced practices,and whether JIH could have better grain yield performance over IH under input-reduced *** this study,three JIH varieties and three IH varieties were compared in grain yield and their related morpho-physiological traits under two cultivation modes,i.e.,conventional high-yielding method(CHYM)and double reductions in nitrogen rate and planting density(DRNP).Our results showed that JIH had 8.3 and 13.3%higher grain yield over IH under CHYM and DRNP,*** superior grain yield of JIH over IH under DRNP was mainly attributed to larger sink size and improved sink filling *** main morpho-physiological traits were concluded for better yield performance of JIH over IH under ***,JIH had the reduced unproductive tillers growth,indicated by a higher percentage of productive tillers and the percentage of effective leaf area index(LAI)to total LAI at heading ***,a synergistic increase in biomass accumulation and harvest index were achieved of JIH,supported by higher biomass accumulation and leaf area duration during the main growth periods,and improved non-structural carbohydrate(NSC)remobilization after ***,JIH had an improved canopy structure,showing as higher leaf area of upper three leaves and lower light extinction *** results suggested that improved morpho-physiological traits of JIH could lead to better grain yield performance over IH under input-reduced practices.

关键词： japonica/indica hybrid rice grain yield input-reduced practices

Mathematics of multi-agent learning systems at the interface of game theory and artificial intelligence

在线全文

学校读者我要写书评

暂无评论

Science China(Information Sciences) 2024年第6期67卷 445-447页

作者： long WANG Feng FU Xingru chen Center for Systems and Control College of EngineeringPeking University Center for Multi-Agent Research Institute for Artificial IntelligencePeking University Department of Mathematics Dartmouth College Department of Biomedical Data Science Geisel School of Medicine at Dartmouth School of Science Beijing University of Posts and Telecommunications

Evolutionary game theory (EGT) and artificial intelligence(AI) are two fields that,at first glance,might seem distinct,but they have notable connections and *** former focuses on the evolution of behaviors (or strategies)in a population,where individuals interact with others and update their strategies based on imitation (or social learning).

关键词：