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Increased L-arginine Production by Site-directed Mutagenesis of N-acetyl-L-glutamate Kinase and pro B Gene Deletion in Corynebacterium crenatum

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Biomedical and Environmental Sciences 2015年第12期28卷 864-874页

作者： ZHANG Bin WAN Fang QIU Yu Lou chen xue lan TANG Li chen Jin Cong XIONG Yong Hua Key Laboratory of Functional Small Organic Molecule Ministry of Educationand School of Life ScienceJiangxi Normal University State Key Laboratory of Food Science and Technology Nanchang University

Objective In Corynebacterium crenatum,the adjacent D311 and D312 of N-acetyl-L-glutamate kinase(NAGK),as a key rate-limiting enzyme of L-arginine biosynthesis under substrate regulatory control by arginine,were initially replaced with two arginine residues to investigate the L-arginine feedback inhibition for *** NAGK enzyme expression was evaluated using a plasmid-based *** recombination was employed to eliminate the pro *** The IC50 and enzyme activity of NAGK M4,in which the D311 R and D312 R amino acid substitutions were combined with the previously reported E19 R and H26 E substitutions,were 3.7-fold and 14.6% higher,respectively,than those of the wild-type *** M4 was successfully introduced into the *** MT genome without any genetic markers;the L-arginine yield of *** MT-M4 was 26.2% higher than that of *** *** further improve upon the L-arginine yield,we constructed the mutant *** MT-M4 ?pro *** optimum concentration of L-proline was also investigated in order to determine its contribution to L-arginine *** L-proline was added to the medium at 10 mmol/L,the L-arginine yield reached 16.5 g/L after 108 h of shake-flask fermentation,approximately 70.1% higher than the yield attained using *** *** Feedback inhibition of L-arginine on NAGK in *** is clearly alleviated by the M4 mutation of NAGK,and deletion of the pro B in *** from MT to M4 results in a significant increase in arginine production.

关键词： Corynebacterium crenatum N-acetyl-L-glutamate kinase Site-directed mutagenesis L-arginine proB

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蛋白质赖氨酸残基的琥珀酰化修饰

中国生物化学与分子生物学报 2018年第12期34卷 1272-1279页

作者：李蓉陈雪岚江西师范大学生命科学学院南昌330096

蛋白质的琥珀酰化修饰是一种普遍存在于真核生物和原核生物中的翻译后修饰。修饰的蛋白质遍及细胞膜、细胞质基质、各种细胞器及细胞核等细胞的各个部分,它们参与了细胞内包括糖代谢、三羧酸循环和脂肪酸代谢等各种代谢反应,与生命体的... 详细信息

蛋白质的琥珀酰化修饰是一种普遍存在于真核生物和原核生物中的翻译后修饰。修饰的蛋白质遍及细胞膜、细胞质基质、各种细胞器及细胞核等细胞的各个部分,它们参与了细胞内包括糖代谢、三羧酸循环和脂肪酸代谢等各种代谢反应,与生命体的活动息息相关。本文综述了琥珀酰化蛋白质活性变化、修饰位点周围氨基酸的特异性及空间结构的分析、亚细胞分布情况、琥珀酰化与乙酰化之间的相互作用及碳源和生长阶段对蛋白质琥珀酰化水平的影响等内容,以期为后续蛋白质的琥珀酰化科研提供一定的参考。

关键词：赖氨酸琥珀酰化去琥珀酰化翻译后修饰乙酰化作用

Unraveling the therapeutic potential of Calculus Bovis in liver cancer:A novel step for targeted cancer treatment

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World Journal of Gastroenterology 2025年第3期31卷 127-129页

作者： Xing-Yu chen Xiang lan Department of Hepatobiliary Surgery The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China

Hepatocellular carcinoma is one of the leading causes of cancer-related deaths globally,and effective treatments are urgently *** present study aimed to investigate the inhibitory effect of Calculus Bovis(CB)on liver cancer and the underlying *** inhibited M2 tumor-associated macrophage polarization and modulated the Wnt/β-catenin signaling pathway,thereby suppressing the proliferation of liver cancer *** inhibitory effect on liver cancer growth was confirmed by both in vivo and in vitro experiments(detailed by Huang et al).The present study provides a theoretical basis for the application of CB for the treatment of liver cancer,providing new avenues for liver cancer treatment.

关键词： Calculus Bovis Liver cancer M2 tumor-associated macrophage polarization Wnt/β-catenin Pathway Tumor microenvironment

Expression and Characterization of ArgR, An Arginine Regulatory Protein in Corynebacterium crenatum

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Biomedical and Environmental Sciences 2014年第6期27卷 436-443页

作者： chen xue lan ZHANG Bin TANG Li JIAO Hai Tao XU Heng Yi XU Feng XU Hong WEI Hua XIONG Yong Hua Key Laboratory of Functional Small Organic Molecule Ministry of Education Jiangxi Normal University State Key Laboratory of Food Science and Technology Nanchang University Ocean NanoTech LLC

Objective Corynebacterium crenatum MT, a mutant from C. crenatum AS 1.542 with a lethal argR gene, exhibits high arginine production. To confirm the effect of ArgR on arginine biosynthesis in C. crenatum, an intact argR gene from wild-type AS 1.542 was introduced into C. crenatum MT, resulting in C. crenatum MT. sp, and the changes of transcriptional levels of the arginine biosynthetic genes and arginine production were compared between the mutant strain and the recombinant strain. Methods Quantitative real-time polymerase chain reaction was employed to analyze the changes of the related genes at the transcriptional level, electrophoretic mobility shift assays were used to determine ArgR binding with the argCJBDF, argGH, and carAB promoter regions, and arginine production was determined with an automated amino acid analyzer. Results Arginine production assays showed a 69.9% reduction in arginine from 9.01±0.22 mg/mL in C. crenatum MT to 2.71±0.13 mg/mL （P〈0.05） in C. crenatum MT. sp. The argC, argB, argD, argF, argJ, argG, and carA genes were down-regulated significantly in C. crenatum MT. sp compared with those in its parental C. crenatum MT strain. The electrophoretic mobility shift assays showed that the promoter regions were directly bound to the ArgR protein. Conclusion The arginine biosynthetic genes in C crenatum are clearly controlled by the regulator ArgR, and intact ArgR in C. crenatum MT results in a significant descrease in production. negative arginine production.

关键词： Corynebacterium crenatum ArgR protein Arginine biosynthetic genes Real-time PCR ElectrophoretJc mobility shift assay

Effects of Shenqi Xiangyi granules in advanced gastric cancer chemotherapy

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 169-175页

作者： Xiao-Jing Shi Yu Song xue-xue Liang Ting chen Huang-Yu Hao xue Han Ya-Nan chen Department of Oncology Zhangjiagang First People's HospitalSuzhou 215600Jiangsu ProvinceChina

BACKGROUND Owing to the absence of specific symptoms in early-stage gastric cancer,most patients are diagnosed at intermediate or advanced *** a result,treatment often shifts from surgery to other therapies,with chemotherapy and targeted therapies being the primary options for advanced gastric cancer treatment.A total of 116 patients with advanced gastric cancer,admitted from January 2021 to December 2023,were selected and divided into two groups of 58 each using the random number table *** control group received FOLFOX4 chemothe-rapy(oxaliplatin+calcium+folinate+5-fluorouracil)combined with intravenous *** observation group received the same treatment as the control group,supplemented by oral administration of Senqi Shiyiwei *** groups underwent treatment cycles of 3 weeks,with a minimum of two *** therapeutic efficacy,immune mechanisms,and treatment-related toxicity and side effects were compared between the *** objective remission rate in the observation group(55.17%)was higher than that of the control group(36.21%)(P0.05).CONCLUSION Adjuvant therapy with Shenqixian granules may enhance the efficacy of simudizumab combined with FOLFOX4 chemotherapy in advanced gastric cancer and the immune function by increasing immune cell counts,making it a valuable option in clinical treatment.

关键词： Sindilizumab FOLFOX4 chemotherapy Advanced gastric cancer Ginseng Qi Xiangyi granule Clinic treatment

Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine

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Biomedical and Environmental Sciences 2014年第2期27卷 134-137页

作者： REN Mei Ling chen xue lan LI Chao Hui XU Bo LIU Wen Juan XU Heng Yi XIONG Yong Hua State Key Laboratory of Food Science and Technology Nanchang University Key Laboratory of Functional Small Organic Molecule Ministry of Education Jiangxi Normal University Wuxi Zodoboer Biotech. Co. Ltd.

A strip reader based lateral flow immunoassay （LFIA） was established for the rapid and quantitative detection of ractopamine （RAC） in swine urine. The ratio of the optical densities （ODs） of the test line （AT） to that of the control line （Ac） was used to effectively minimize interference among strips and sample variations. The linear range for the quantitative detection of RAC was 0.2 ng/mL to 3.5 ng/mL with a median inhibitory concentration （IC50） of 0.59＋0.06 ng/mL. The limit of detection （LOD） of the LFIA was 0.13 ng/mL. The intra-assay recovery rates were 92.97%, 97.25%, and 107.41%, whereas the inter-assay rates were 80.07%, 108.17%, and 93.7%, respectively.

关键词： RAC Lateral Flow Immunoassay for Quantitative Detection of Ractopamine in Swine Urine Figure AT

硝酸盐呼吸和厌氧氨氧化的机制及其应用研究进展

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生命科学 2021年第4期33卷 428-437页

作者：朱凌峰陈雪岚江西师范大学生命科学学院南昌330022

硝酸盐呼吸过程广泛存在于厌氧及兼性厌氧菌中。当硝酸盐存在时,微生物在微氧或缺氧条件下,以硝酸盐作为电子受体,与电子传递呼吸链过程相耦合,产生能量并用于维持细胞的基本生命活动。硝酸盐的呼吸过程对污水处理、土壤氮保持、微生物... 详细信息

硝酸盐呼吸过程广泛存在于厌氧及兼性厌氧菌中。当硝酸盐存在时,微生物在微氧或缺氧条件下,以硝酸盐作为电子受体,与电子传递呼吸链过程相耦合,产生能量并用于维持细胞的基本生命活动。硝酸盐的呼吸过程对污水处理、土壤氮保持、微生物氮循环具有重要作用,研究硝酸盐呼吸对环境保护具有重要意义。该文对反硝化作用、硝酸盐异化还原为铵这两种硝酸盐呼吸和以硝酸盐为间接电子受体的厌氧氨氧化过程进行了综述,阐述了与硝酸盐呼吸、厌氧氨氧化有关的氧化还原酶基因簇以及相应的氧化还原酶的结构和功能,并对两者研究现状和未来发展方向进行了总结与展望。

关键词：硝酸盐呼吸电子受体硝酸盐异化还原为铵反硝化作用厌氧氨氧化

Targeting cholesterol trafficking to mitigate axonal degeneration in hereditary spastic paraplegia

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Neural Regeneration Research 2025年第5期20卷 1397-1398页

作者： Zhenyu chen xue-Jun Li Department of Biomedical Sciences University of Illinois College of Medicine RockfordRockfordILUSA Department of Bioengineering University of Illinois at ChicagoChicagoILUSA

Axonal degeneration underlies many debilitating diseases including hereditary spastic paraplegia(HSP),a genetically and clinically diverse group of disorders characterized by spasticity and weakness of the lower *** is one significant cause of chronic neurodisability due to the lack of effective treatments and a wide range of onset ages from early childhood to 70 years.

关键词： degeneration diseases hereditary

旅游影响下少数民族节日的文化适应与重构——基于哈尼族长街宴演变的分析

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地理研究 2011年第5期30卷 835-844页

作者：唐雪琼钱俊希陈岚雪西南林业大学生态旅游学院昆明650224 英国爱丁堡大学地学学院

在民族文化旅游发展的大背景下,少数民族节日传统文化在与外来文化的互动与碰撞过程中,不断经历着"地方传统"与"现代发展"的冲突与融合。本文通过元阳哈播、绿春两地长街宴节日活动的实地调研,研究哈尼族长街宴这一节日活动在旅游发展... 详细信息

在民族文化旅游发展的大背景下,少数民族节日传统文化在与外来文化的互动与碰撞过程中,不断经历着"地方传统"与"现代发展"的冲突与融合。本文通过元阳哈播、绿春两地长街宴节日活动的实地调研,研究哈尼族长街宴这一节日活动在旅游发展中的演变过程。研究指出:在旅游活动的影响下,在地方政府的引导或主导下,哈尼族长街宴为了适应游客传承的来自现代社会的主流文化,经历了地方文化认同、文化适应与文化重构,被包装、重塑成旅游节庆产品,从哈尼村寨走向旅游市场,从地方认同走向世界文化。

关键词：文化适应文化重构少数民族节日文化长街宴