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The general public’s ability to operate automated external defi brillator: A controlled simulation study

World Journal of Emergency Medicine 2020年第4期11卷 238-245页

作者： Xue-jie Dong Lin Zhang Yue-lin Yu Shu-xiao Shi xiao-chen yang xiao-qian Zhang Shuang Tian Helge Myklebust Guo-hong Li Zhi-jie Zheng School of Public Health Shanghai Jiao Tong UniversityShanghaiChina School of Medicine Shanghai Jiao Tong UniversityShanghaiChina Laerdal Medical StavangerNorway School of Public Health Peking UniversityBeijingChina

BACKGROUND:Automated external defibrillators(AEDs)enable laypeople to provide early defi brillations to patients undergoing cardiac arrest,but scant information is available on the general public’s ability to use *** study assessed the ability of laypeople to operate AEDs,the effect of a 15-minute training,and whether skills differed by ***:From May 1 to December 31,2018,a prospective simulation study was conducted with 94 laypeople aged 18–65 years(32 aged 18–24 years,34 aged 25–54 years,and 28 aged 55–65 years)with no prior AED *** participants’AED skills were assessed individually pretraining,post-training,and at a three-month follow-up using a simulated cardiac arrest *** critical actions and time intervals were evaluated during the AED operating ***:Only 14(14.9%)participants(eight aged 18–24 years,four aged 25–54 years,and two aged 55–65 years)successfully delivered defi brillations before *** operation errors were more likely to occur among the participants aged 55–65 years than among other age *** training,the proportion of successful defi brillations increased signifi cantly(18–24 years old:25.0%vs.71.9%,P<0.01;25–54 years old:11.8%vs.70.6%,P<0.01;55–65 years old:7.1%vs.67.9%,P<0.01).After three months,26.1%of the participants aged 55–65 years successfully delivered defi brillations,which was signifi cantly lower than that of participants aged 18–24 years(54.8%)and 25–54 years(64.3%)(P=0.02).There were no differences in time measures among three age groups in each ***:The majority of untrained laypeople cannot effectively operate *** frequent training and refresher courses are crucial to improve AED skills.

关键词： Automated external defi brillator Cardiac arrest General public Training Simulation

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Characteristics of nucleotides and amino acids in cDNA sequence of xyloglucan endotransglycosylase cloned from Anthocephalus chinensis

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Forestry Studies in China 2011年第1期13卷 45-51页

作者： LI Wei ZHU Song-lin LI Na chen xiao-yang Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education National Engineer- ing Laboratory for Forest Tree Breeding College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China State-owned Forest Administration of Lvliangshan Mountain Linfen 041000 P. R. China College of Forestry South China Agricultural University Guangzhou 510642 E R. China

Xyloglucan endotransglycosylase is an identified relaxation factor with functions of easing and extending of plant cell *** activities are directly related to plant growth and elongation of *** chinensis is a tall and fast growing evergreen tree *** cloned the full cDNA sequence of AcEXT genes which is abundantly expressed in the cambium of *** sequence analysis of nucleotides and amino acids revealed the presence of a 1396 bp full cDNA sequence,including a 960 bp complete open reading frame（ORF） encoding a 320 amino acid *** deduced amino acid sequence of AcXET was homologous to the other known XET proteins and contained the conserved EIDFE catalytic site which was specific to all the *** data should serve as a foundation for further insight into AcXET gene molecular mechanisms during wood formation and cell wall engineering of woody plants.

关键词： cloning XET gene sequence analysis Anthocephalus chinensis

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Polyploid induction of Lespedeza formosa by colchicine treatment

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Forestry Studies in China 2007年第4期9卷 283-286页

作者： Li Wei Hu Dong-nan Li Hui chen xiao-yang College of Biological Sciences and Biotechnology Beijing Forestry University Beijing 100083 P. R. China College of Landscape Architecture and Art Jiangxi Agriculture University Nanchang 330045 P. R. China

Polyploid induction has been conducted by different colchicines concentrations with seed, hypocotyl and apices of Lespedezaformosa. Morphological variation and amounts of chromosome about the polyploidy have also been analyzed. Results show that the best material for polyploid induction of L. formosa is the new apices from seed germination, with an induction rate of 44.4% at 0.1% colchicines concentration and 36 hours of treatment time. Compared with normal diploid plants, the polyploidy plants of L. formosa inducted in our experiments have short stature and stems with thick and wide leaves. Cytological studies show that the changed seedlings, whose morphology has changed dramatically, are tetraploid, with 44 （2n = 4x = 44） chromosomes in the somatic cells.

关键词： Lespedeza formosa colchicines polyploid induction morphological variation

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Expression analysis of chlorophyllid a binding protein a secretory, red fluorescence protein in the midgut of silkworm, Bombyx mori

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Insect Science 2014年第1期21卷 20-30页

作者： Lin chen Rui yang xiao-Long Hu Xing-Wei Xiang xiao-Feng Wu Institute of Sericulture and Apiculture College of Animal Sciences Zhejiang University Hangzhou China

Chlorophyllid a binding protein （ chbp） was recently characterized by its ability to bind the prosthetic group of chlorophylls and little information is known regarding its expression. In the present study, we found that chpb was expressed highly and exclusively in the midgut of silkworm, Bombyx mori. The expression level of chbp was very high in the newly molted fifth instar larvae followed by gradual decline in the same instar. Our results demonstrated that CHBP was a secretory protein and located mainly in the apical of midgut epithelial cells. Real-time polymerase chain reaction analysis results showed that chpb highly expressed in the anterior midgut, threefold and sixfold higher compared with that of the middle midgut and posterior midgut, respectively, and chpb expression declined in darkness. In addition, the expression of chbp was affected by high-dose virus or bacterium infection.

关键词： Bombyx mori chlorophyllid binding protein expression midgut

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Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector

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Journal of Integrative Agriculture 2013年第5期12卷 803-814页

作者： Nazim Hussain Zahra Jabeen LI Yuan-long chen Ming-xun LI Zhi-lan GUO Wan-li Imran Haider Shamsi chen xiao-yang JIANG Li-xi Key Laboratory of Crop Germplasm Resources of Zhejiang Province College of Agriculture and BiotechnologyZhejiang University

The variation among Chinese genotypes of Brassica napus L. for seed tocopherols content and their analysis using gas chromatography has not been comprehensively reported till to date. In the present study, the tocopherol contents of four Chinese genotypes of Brassica napus L., namely, Gaoyou 605, Zhejiang 619, Zheshuang 758, and Zheshuang 72, were evaluated using three modified sample preparation protocols （P1, P2, and P3） for tocopherol extraction. These methods were distinguished as follows. Protocol one （P1） included the evaporation of solvent after extraction without silylation. Protocol two （P2） followed the direct supernatant collection after overnight extraction without drying and silylation. Protocol three （P3） included trimethylsilylation with N,O-bis（trimethylsilyl） trifluoroacetamide. Genotypic comparison of tocopherol and its isoforms revealed that Gaoyou 605 was dominant over the other genotypes with （140.5＋ 10.5）, （316.2＋ 9.2）, and （559.1＋ 24.3） ~tg g-~ of seed meal ct-, 7-, and total （T-） tocopherol, respectively, and a 0.44＋0.04 ^- to 7-tocopherol ratio. The comparison of the sample preparation protocols, on the other hand, suggests that P3 is the most suitable method for the tocopherol extraction from Brassica oilseeds and for the analysis of tocopherols using gas chromatography flame ionization detector （GC-FID）. Trimethylsilylation is the key step differentiating P3 from P1 and P2. Variations detected in tocopherol contents among the Chinese rapeseed （B. napus） genotypes signify the need to quantify a wide range of rapeseed germplasm for seed tocopherol dynamics in short and crop improvement in long.

关键词： Brassica napus L. genotypic variation tocopherol analysis gas chromatography flame ionization detector

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Isolation and sequence analysis of a Dof protein gene(PtDof1) in Populus

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Forestry Studies in China 2009年第2期11卷 93-98页

作者： XIN Bei chen xiao-yang OUyang Kun-xi PIAN Rui-qi LI Wei Key Laboratory for Genetics and Breeding of Forest Trees and Ornamental Plants of Ministry of Education Beijing Forestry University Beijing 100083 P. R. China College of Forestry South China Agricultural University Guangzhou 200064 P. R. China

Both cDNA and DNA clones of PtDof1 （GenBank Accession No. FJ402844 and FJ402845） were isolated from plants grown in tissue culture ofPopulus tornentosa. The DNA sequence is 1597 bp including two exons and one intron. The cDNA is 969 bp in length with a 765 bp open reading frame which is capable of encoding 255 amino acids. The deduced amino acids sequence of the PtDofl protein shares 65%, 56% and 55% identity with Vitis vinifera （CAO48618）, Nicotiana tabacum （CAA08755） and Glycine max （ABI 16022） Dof protein by blast analysis in GenBank. Phylogenic analysis suggests PtDof1 gene could belong to the Dofgene family. PtDofl protein contains an unusual conserved single zinc finger with the pattern of C-X2-C-X21-C-X2-C, which may play a functional role in tissue-specific expression and possibly the auxin response of endogenous plant genes.

关键词： Populus tomentosa Dof gene family PtDof1 cloning and sequencing

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Design, assembly, and pre-commissioning of cryostat for 3W1 superconducting wiggler magnet

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Nuclear Science and Techniques 2020年第11期31卷 78-92页

作者： Miao-Fu Xu Xiang-Zhen Zhang Rui Ye Fu-San chen xiao-chen yang Tong-Xian Zhao Shao-Peng Li Xian-Jing Sun Liang-Rui Sun Chang-cheng Ma Rui Ge Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Key Laboratory of Particle Acceleration Physics and Technology Chinese Academy of SciencesBeijing 100049China Center for Superconducting RF and Cryogenics Institute of High Energy PhysicsBeijing 100049China University of Chinese Academy of Sciences Beijing 100049China

One of the most important devices for the High Energy Photon Source Test Facility project,the 2.6 T 32-pole 3W1 superconducting wiggler,was designed by the Institute of High Energy Physics(IHEP);its magnetic gap is 68 mm,and its storage energy is 286 *** will be installed at the storage ring of the Beijing Electron Positron Collider Upgrade Project at the IHEP to replace the old permanent *** primary purpose of the cryostat is to create a safe and reliable system and realize long-term operation with zero liquid helium *** maintain liquid helium temperature,four identical two-stage cryocoolers are placed symmetrically at the wiggler *** cryostat has only one 60 K thermal shield,which is used to reduce the heat load to the liquid helium *** cryostat has several novel features,including a suspension system with little heat leakage that is self-centered during cooling of the cryostat,a special copper liner and high-efficiency condensers,three pairs of binary current leads,and three-level safety *** cryogenic system has been cooled three times,and the residual cooling capacity is approximately 0.41 W at 4.2 K without current.

关键词： Cryostat Cryocooler Superconducting wiggler Superconducting insertion device

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Intermittent hypoxia and isoniazid plus rifampicin affect hepatic ultrastructure in mice

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Chinese Medical Journal 2011年第23期124卷 4034-4038页

作者： WU Run-hua ZENG Yi-ming chen xiao-yang Department of Pulmonary Medicine Second Affiliated Hospital ol Fujian Medical University Quanzhou Fujian 362000 China

Background Chronic intermittent hypoxia is the most important pathophysiologic feature of sleep apnea syndrome. The present study aimed to determine whether chronic intermittent hypoxia, which is associated with sleep apnea syndrome, can cause or increase damage to liver cell ultrastructure induced by isoniazid and rifampicin in mice. Methods Based on a 2x2 full factorial design consisting of two factors of chronic intermittent hypoxia and isoniazid plus rifampicin, 32 male C57B6J mice were randomized into the control group, the chronic intermittent hypoxia group, the isoniazid plus rifampicin group, and the chronic intermittent hypoxia ＋ isoniazid plus rifampicin group. Twelve weeks after treatment, we examined the ultrastructure of liver cells and quantitatively analyzed mitochondrial morphology in C57B6J mice. Results Chronic intermittent hypoxia did not significantly affect the ultrastructure of liver cells. The main effect of chronic intermittent hypoxia did not lead to an increase of mean profile area or mean perimeter of mitochondria, and a decrease of numerical density on area of mitochondria （all P 〉0.05）. Isoniazid plus rifampicin significantly affected liver cell ultrastructure. The main effect of isoniazid plus rifampicin resulted in an increase of mean profile area and mean perimeter of mitochondria, and a decrease of numerical density on area of mitochondria （all P 〈0.05）. Moreover, there was a positive interaction among the chronic intermittent hypoxia and the isoniazid plus rifampicin groups for mean profile area, mean perimeter, and numerical density on area of mitochondria （all P〈0.05）. Conclusion Chronic intermittent hypoxia and isoniazid plus rifampicin treatment lead to synergistic liver cell ultrastructural injury.

关键词： sleep apnea syndrome chronic intermittent hypoxia ultrastructure

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Highly conductive and stable iodine doped argyrodite electrolyte for all-solid-state lithium batteries

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Journal of Energy Chemistry 2025年第1期100卷 50-58页

作者： Gaozhan Liu Jing Zhang Jing yang Jinghui chen Xingyue xiao Xiayin Yao Ningbo Institute of Materials Technology and Engineering Chinese Academy of Sciences Center of Materials Science and Optoelectronics Engineering University of Chinese Academy of Sciences

Lithium argyrodites with high ionic conductivity and low cost are considered as one of the most prospective solid electrolytes for all-solid-state lithium ***,the poor chemical stability and compatibility with lithium metal limit their ***,Li5.4PS4.4Cl1.4I0.2solid electrolyte with high ionic conductivity of 11.49 m S cm-1and improved chemical stability is synthesized by iodine *** ultra-thin Li5.4PS4.4Cl1.4I0.2membrane with thickness of 10μm can be obtained by wet coating process,exhibiting a high ionic conductivity of 2.09 mS cm-1and low areal resistance of 1.17Ω***,iodine doping could in-situ form LiI at the lithium/electrolyte interface and improve the critical current density of Li5.4PS4.4Cl1.6from 0.8 to 1.35 mA *** resultant LiCoO2/Li5.4PS4.4Cl1.4I0.2/Li battery shows excellent cycling stability at 1 C,with a reversible specific capacity of 110.1 mA h g-1and a retention of 80.5% after 1000 *** addition,the assembled LiCoO2/Li5.4PS4.4Cl1.4I0.2membrane/Li pouch cell delivers an initial discharge capacity of 110.4 mA h g-1and 80.5% capacity retention after 100 cycles.

关键词： Argyrodite electrolytes Iodine doping Ionic conductivity Stability All-solid-state lithium battery

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Cloning of XET gene from Anthocephalus chinensis and its plant expression vector construction

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Forestry Studies in China 2010年第2期12卷 79-84页

作者： MA Sheng-jun ZHU Song-lin LI Wei OUyang Kun-xi LI Na chen xiao-yang Key Laboratory for Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education National Engineering Laboratory for Forest Tree Breeding Beijing Forestry University Beijing 100083 P. R. China State-owned Forest Administration of Lvliangshan Mountain Linfen 041000 P. R. China Forestry Research Institute of Hebei Province Shijiazhuang 050061 P. R. China College of Forestry South China Agricultural University Guangzhou 200064 P. R. China

A full-length cDNA sequence of xyloglucan endotransglycosylase gene （XET）, abundantly expressed in the cambium of Anthocephalus chinensis was cloned by conserved PCR, rapid-amplification of cDNA ends and by chromosome walking. Analytical results of the DNA sequence show that a 912 bp complete open reading frame （ORF） encoded a 303-amino acid protein was in the 1205 bp full cDNA sequence. The deduced amino acid sequence of AcXET, which contained the conserved specific EIDFE catalytic site sequence to XETs was homologous to the other known XET proteins. In order to study the gene function of AcXET and obtain transgenic plants, a plant expression vector pBIAcXET was constructed by recombinating the AcXET fragment from the cloning vector pMD19AcXET and the binary vector pBI121 between the XbaI and SmaI sites. The fragment ofAcXET gene was inserted between the CaMV 35S promotor and the coding region of the GUS gene in pBI121. The identification results show that the plant expression binary vector pBIAcXET was constructed successfully. These results lay the foundation for studying the molecular mechanism ofAcXET gene during wood formation.

关键词： cDNA cloning sequence analysis AcXET gene plant expression vector

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