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Exosome-Transmitted miR-2.4-5p Promotes Colorectal Cancer Cell Proliferation via Targeting ULK2.in p53-Dependent Manner

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biomedical and Environmental sciences 2024年第1期37卷 71-84页

作者： YANG Le Mei ZHENG Qi LIU Xiao Jia LI xian xian Veronica Lim {2. Qi ZHAO Zhong Hua WANG Shu Yang Department of Pathology School of Basic MedicineFudan UniversityShanghai 200032China Department of Pathology Dunedin School of MedicineUniversity of OtagoDunedin 9054New Zealand Department of Pathology Huashan HospitalFudan UniversityShanghai 200040China Shanghai Cancer Institute Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Teladoc Health Inc Shanghai 200335China Shanghai Medical College Fudan UniversityShanghai 200032China Shanghai Key Laboratory of Medical Imaging Computing and Computer Assisted Intervention Shanghai 200032China

Objective To investigate the role and molecular mechanism of exosomal miR-2.4-5p in colorectal cancer(CRC).Methods The miR-2.4-5p expression in CRC patient tissues and cell-derived exosomes was measured by laser capture microdissection and qRT-PCR,***-luciferase reporter gene assay was used to determine the target gene of *** protein expressions of p53 and unc-51 like kinase 2.ULK2.in CRC cells were detected by western *** cytometry was used to detect cell cycle and *** proliferation was measured by CCK8 and EdU *** The miR-2.4-5p expression was upregulated in CRC tissues and increased progressively with the rise of CRC *** cells secreted extracellular miR-2.4-5p mainly in an exosome-dependent manner,and then miR-2.4-5p could be transferred to surrounding tumor cells to regulate cell proliferation in the form of autocrine or ***,ULK2.was characterized as a direct target of miR-2.4-5p and was downregulated in CRC ***,ULK2.inhibited CRC cell proliferation in a p53-dependent ***,exosome-derived miR-2.4-5p partially reversed the proliferation regulation of ULK2.on CRC *** Our findings demonstrate that exosome-transmitted miR-2.4-5p promotes p53-dependent cell proliferation by targeting ULK2.in CRC,which may offer promising targets for CRC prevention and therapy.

关键词： miR-2.4-5p Exosome ULK2 p53 Cell proliferation Colorectal cancer

Mass spectrometry analysis of melanoma related O-glycans in sera

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Science china Chemistry 2010年第4期53卷 807-811页

作者： BAI chen1,4, WU Gang1 & ZHAO Liang2.3 1Department of Life Science, fudan university, Shanghai 2.0433,{2. 2.epartment of Chemistry, fudan university, Shanghai 2.0433,{2. 3institutes of biomedical sciences, fudan university, Shanghai 2.0433,{2. 4Department of Food Science, Shanghai Business School, Shanghai 2.02.5,{2. 1. Department of Life Science Fudan University Shanghai 200433 China4. Department of Food Science Shanghai Business School Shanghai 200235 China2. Department of Chemistry Fudan University Shanghai 200433 China3. Institutes of Biomedical Sciences Fudan University Shanghai 200433 China

Glycomics comparison was carried out by screening melanoma serum biomarkers between C57 mice with and without B16 implanted. O-glycans were released from 10 μL sera by β-elimination, purified by Graphitized Carbon Cartridge Solid Phase Extraction (GCC-SPE) and analyzed by MALDI-QIT-TOF-MS. MS raw data were acquired and exported by Launchpad software. MATLAB was then applied for further data analysis. 10 Glycans were considered to have stable changes after B16 implantation and 5 of them were under structural analysis via MS/MS.

关键词： mass spectrometry glycomics melanoma biomarkers

WNT5A Regulated by miR154-5p is Associated with Angiopoiesis and Invasion of Placental Site Trophoblastic Tumor (PSTT)

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biomedical and Environmental sciences 2024年第4期37卷 423-427页

作者： ZHANG Sai chen Zhi xian ZHANG Mo DU Yan ZHOU Jia Yi WU Jing YU Yin Hua CAO Qi ZHAO Hong Bo Obstetrics and Gynecology Hospital Fudan UniversityShanghai 200011China Shanghai Key Laboratory of Female Reproductive Endocrine Related Diseases Shanghai 200011China

Placental site trophoblastic tumor(PSTT),a rare gestational trophoblastic neoplasm(GTN),exhibits slow growth,late-onset metastasis,and chemotherapy insensitivity[1,2..The invasive capacity of trophoblasts induces angiogenesis in PSTT,yet the specific mechanism remains unclear[3].PSTT is typically resistant to chemotherapy and hysterectomy stands as the primary therapeutic choice for patients with an overall cure rate of approximately 75%-80%[4,5].Therefore,investigating the precise emergence and progression of PSTT is crucial,aiming to identify novel targets for diagnosis and therapy.

关键词： chemotherapy diagnosis metastasis

Development of Two Scalable Synthesis of 4-Amino-5-aminomethyl-2.methylpyrimidine（GDA）:Key Intermediate for Vitamin B1

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Development of Two Scalable Synthesis of 4-Amino-5-aminometh...

2.11年全国药物化学学术会议——药物的源头创新

作者： Lei Zhao~(a,b),Xiao-Dong Ma~a,Fen-Er chen~(a,b,*) a Department of Chemistry,fudan {2.,2.0 Handan Road,Shanghai 2.0433, P.R.of b china,and institutes of biomedical sciences,fudan {2.,305 Fenglin Road,Shanghai 2.0032.P.R.of china

＜正＞Two scalable processes for the synthesis of 4-amino-5-aminomethyl-2.methylpyrimidine（2.GDA） are *** the first approach,the less expensive 2.cyanoacetamide was reacted with Vilsmeier reagent to afford enamine 1...

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Structural insights into a dual-specificity histone demethylase ceKDM7A from Caenorhabditis elegans

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Structural insights into a dual-specificity histone demethyl...

第十七届国际生物物理大会;第十二届中国生物物理大会

作者： Ying Yang~(1,2.3),Lu Zhou~5,Hanqin Lin~6,Degui chen~6,Yanhui Xu~(1,2.3),Lulu Hu~(2.3),Ping Wang~(2.3),Haifeng Hou~4,Yan Lin~(2.3),Yi Liu~(2.3) 1.Cancer Institute,Shanghai Cancer Center,fudan {2. Department of Oncology,Shanghai Medical College,fudan {2.,Shanghai,china 2.School of Life sciences,fudan {2.,2.0 Han-Dan Road,Shanghai,china 3.institutes of biomedical sciences,fudan {2.,Shanghai,china 4.Beijing Synchrotron Radiation Facility,Institute of High Energy Physics,Chinese Academy of sciences, Beijing,china 5.School of Pharmacy,fudan Univercity,South Chemistry Building,Room 315,82. Zhangheng Road, Shanghai,china 6.State Key Laboratory of Molecular Biology,Shanghai institutes for Biological sciences,Chinese Academy of sciences,Shanghai,china

Histone lysine nethylation can be removed by JmjC domain-containing proteins in a sequence- and methylation-state-specific ***,how substrate specificity is determined and how the enzymes are regulated were largely ***... 详细信息

关键词： histone methylation demethylase PHD Jmjc

Phylogenetics and Evolution of Su(var)3-9 SET Genes in Land Plants:Rapid Diversification in Structure and Function

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Phylogenetics and Evolution of Su(var)3-9 SET Genes in Land ...

第十二届全国植物基因组学大会

作者： Xinyu Zhu~(1,2.,Hong Ma~(3,4),Zhiduan chen~(1*) 1.State Key Laboratory of Systematic and Evolutionary Botany,Institute of Botany,Chinese Academy of sciences,Beijing 100093,{2. 2.School of Life sciences,Nantong university,Nantong 2.6019,{2. 3.State Key Laboratory of Genetic Engineering, School of Life sciences,Institute of Plant Biology,Center for Evolutionary Biology,institutes of biomedical sciences, fudan university,Shanghai 2.0433,{2. 4.Department of Biology,the Huck institutes of the Life sciences,The Pennsylvania State university,university Park,PA 16802.USA

Plants contain numerous Su(var)3-9 homologues(SUVH) and related(SUVR) *** there have been studies on the evolution of plant Su(var)3-9 SET genes,a systematic evolutionary study including major land plant groups has not been *** orthologs of plant Su(var)3-9 SET protein sequences were retrieved from major representatives of land plants.A novel clustering that included most members analyzed

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UrotensinⅡinhibits the Proliferation but not Differentiation of Cardiac side Population Cells

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UrotensinⅡinhibits the Proliferation but not Differentiatio...

第八届海峡两岸心血管科学研讨会

作者： Hui GONG~1,Hong MA~2.Bo-jiang ZHOU~2.Guo-ping ZHANG~1, Zhi-dan chen~1,Guo-liang JIANG~1,Yuan YAN~2.Chun-jie YANG,Jian WU~1, Jie Yuan~2.Lei LI~1,Jun-bo GE~(1,2.,Yun-zeng ZOU~(1,2.*) (1.institutes of {2. sciences,fudan university,Shanghai 2.0032.china 2.Shanghai Institute of Cardiovascular Diseases,Zhongshan Hospital,fudan university,Shanghai 2.0032.china)

Background Urotensin Ⅱ (U Ⅱ ) induces the development of cardiac remodeling and atherosclerosis by exerting on hypertrophy of cardiomyocytes and mitogenesis of fibroblasts and vascular smooth muscle *** its effect on cardiac side population cell(CSP),one of somatic stem cells,is *** present study examined the influences of U Ⅱ on abilities of CSP to differentiate and *** nd Results CSPs were isolated from neonatal rat hearts by fluorescence - activated cell sorting(FACS) method and cultured with or without U Ⅱ (10,10,10mol·L) *** expression of <- cardiac myosin heavy chain(<-MHC),<-smooth muscle actin(SMA) and Von Willebrand factor(vWF) mRNAs and proteins were analyzed with reverse transcriptional PCR(RT - PCR) and immunofluoresence methods to evaluate abilities of CSPs to differentiate of into cardiomyocytes,smooth muscle cells and endothelial cells,respectively. The proliferation of CSPs was assessed using Luminescent Cell Viability Assay.U Ⅱ (60 nM/kg/day) was also intravenously injected into *** days later,mice were sacrificed and CSPs were isolated from the heart and accounted by *** results revealed that although U Ⅱ didn’t affect the differentiation of cultured CSPs,it did inhibit the proliferation of the cells with up - regulation of phosphorylated c - Jun N -terminal protein kinase(JNK).In vivo experiments also showed that U Ⅱ treatment reduced the number of CSPs in mice comparing with saline *** These data indicate that U Ⅱ reduces the number of CSPs by inhibiting the proliferation of CSPs ossibly through gincrease of JNK phosphorylation.

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Urotensin Ⅱ inhibits the proliferation but not differentiation of cardiac side population cells

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Urotensin Ⅱ inhibits the proliferation but not differentiat...

第十三次全国心血管病学术会议

作者： GONG Hui,MA Hong,ZHOU Bo-jiang,ZHANG Guo-ping,chen Zhi-dan, JIANG Guo-liang,YAN Yuan,YANG Chun-ji,WU Jian,YUAN Jie, LI Lei,GE Jun-bo,ZOU Yun-zeng *institutes of {2. sciences,fudan university,Shanghai 2.0000,china

Objective UrotensinⅡ(UⅡ) induces the development of cardiac remodeling and atherosclerosis by exerting on hypertrophy of cardiomyocytes and mitogenesis of fibroblasts and vascular smooth muscle *** its effect on cardiac side population cell(CSP),one of somatic stem cells,is *** present study examined the influences of UⅡon abilities of CSP to differentiate and *** and Results CSPs were isolated from neonatal rat hearts by fluorescence-activa-

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Biallelic variants in RBM42.cause a multisystem disorder with neurological,facial,cardiac,and musculoskeletal involvement

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Protein & Cell 2024年第1期15卷 52-68页

作者： Yiyao chen Bingxin Yang Xiaoyu Merlin Zhang Songchang chen Minhui Wang Liya Hu Nina Pan Shuyuan Li Weihui Shi Zhenhua Yang Li Wang Yajing Tan Jian Wang Yanlin Wang Qinghe Xing Zhonghua Ma Jinsong Li He-Feng Huang Jinglan Zhang chenming Xu institutes of biomedical {2. Fudan UniversityShanghai 200032China bstetrics and Gynecology Hospital Institute of Reproduction and DevelopmentFudan UniversityShanghai 200011China Intermational Peace Matermity and Child Health Hospital School of MedicineShanghai Jiao Tong UniversityShanghai 200030China Shanghai Key Laboratory of Embryo Original Diseases Shanghai 200030China State Key Laboratory of Cell Biology Shanghai Key Laboratory of Molecular AndrologyShanghai Institute of Biochemistry and Cell BiologyCAS Center for Excellence in Molecular Cell ScienceUniversity of Chinese Academy of SciencesChinese Academy of SciencesShanghai 200031China State Key Laboratory of Rice Biology the Key Laboratory of Molecular Biology of Crop Pathogens and InsectsInstitute of BiotechnologyZhejiang UniversityHangzhou 310058China Verna and Marrs McLean Department of Biochemistry and Molecular Biology Baylor College of MedicineHoustonTX 77030USA Children's hospital of fudan university Shanghai 201102China School of Life Science and Technology Shanghai Tech UniversityShanghai 201210China School of Life Science Hangzhou Institute for Advanced StudyUniversity of Chinese Academy of SciencesHangzhou 310024ZhejiangChina Research Units of Embryo Original Diseases Chinese Academy of Medical Sciences(No.2019RU056)Shanghai 200011China

Here,we report a previously unrecognized syndromic neurodevelopmental disorder associated with biallelic loss-of-function variants in the RBM42.*** patient is a 2.year-old female with severe central nervous system(CNs)abnormalities,hypotonia,hearing loss,congenital heart defects,and dysmorphic facial *** whole-exome sequencing(WEs)reveals that the patient has two compound heterozygous variants,c.304C>T(p.R102.)and c.1312.>A(p.A438T),in the RBM42.gene which encodes an integral component of splicing complex in the RNA-binding motif protein *** p.A438T variant is in the RRM domain which impairs RBM42.pro-tein stability in ***,p.A438T disrupts the interaction of RBM42.with hnRNP K,which is the causa-tive gene for Au-Kline syndrome with overlapping disease characteristics seen in the index *** human R102.or A438T mutant protein failed to fully rescue the growth defects of RBM42.ortholog knockout△FgRbp1 in Fusarium while it was rescued by the wild-type(WT)human RBM42.A mouse model carying Rbm42.compound heterozygous variants,c.2.0C>T(p.Q94*)and c.1306_1308delinsACA(p.A436T),demonstrated gross fetal develop-mental defects and most of the double mutant animals died by ***-seq data confirmed that Rbm42.was involved in neurological and myocardial functions with an essential role in alternative splicing(As).Overall,we present clinical,genetic,and functional data to demonstrate that defects in RBM42.constitute the underlying etiology of a new neurodevelopmental disease which links the dysregulation of global AS to abnormal embryonic development.

关键词： RBM42.gene RNA-binding protein neurodevelopmental disorder Au-Kline syndrome alternative splicing