检索结果-南通市图书馆

The ERF transcription factor LTF1 activates DIR1 to control stereoselective synthesis of antiviral lignans and stress defense in Isatis indigotica roots

引用

Acta Pharmaceutica Sinica B 2024年第1期14卷 405-420页

作者： Ruibing chen Jian Yu Luyao Yu Liang Xiao Ying Xiao Junfeng chen Shouhong Gao Xianghui chen Qing Li Henan Zhang wansheng chen Lei Zhang Department of Pharmaceutical Botany School of PharmacyNaval Medical UniversityShanghai 200433China Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Shanghai Changzheng HospitalNaval Medical UniversityShanghai 200003China School of Medicine Shanghai UniversityShanghai 200433China Institute of Edible Fungi Shanghai Academy of Agricultural SciencesNational Engineering Research Center of Edible FungiKey Laboratory of Edible Fungi Resources and Utilization(South)Ministry of AgricultureShanghai 201403China College of Life Sciences and Medicine Key Laboratory of Plant Secondary Metabolism and Regulation of Zhejiang ProvinceZhejiang Sci-Tech UniversityHangzhou 310018China State Key Laboratory of Dao-di Herbs Beijing 100700China

Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions to protect human *** the mechanisms of stereoselective biosynthesis and response to stresses of lignans is important for the guidance of plant ***,we identified the complete pathway to stereoselectively synthesize antiviral(-)-lariciresinol glucosides in Isatis indigotica roots,which consists of three-step sequential stereoselective enzymes DIR1/2,PLR,and ***1 was further identified as the key gene in respoJanuary 2024nse to stresses and was able to trigger stress defenses by mediating the elevation in lignan ***,the phytohormone-responsive ERF transcription factor LTF1 colocalized with DIR1 in the cell periphery of the vascular regions in mature roots and helped resist biotic and abiotic stresses by directly regulating the expression of *** systematic results suggest that DIR1 as the first common step of the lignan pathway cooperates with PLR and UGT71B2 to stereoselectively synthesize(-)-lariciresinol derived antiviral lignans in *** roots and is also a part of the LTF1-mediated regulatory network to resist *** conclusion,the LTF1-DIR1 module is an ideal engineering target to improve plant Defenses while increasing the content of valuable lignans in plants.

关键词： Lignans Stereoselective synthesis Stress resistance Dirigent protein ERF

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Rapid characterization of non-volatile phenolic compounds reveals the reliable chemical markers for authentication of traditional Chinese medicine Xiang-ru among confusing Elsholtzia species

引用

Chinese Journal of Natural Medicines 2024年第4期22卷 375-384页

作者： ZENG Zhen ZHANG chen HU Jiadong WANG Feiyan WU Ziding WANG Jing ZHANG Jun YANG Shuda chen Junfeng LI Mingming TONG Qi QIU Shi chen wansheng The SATCM Key Laboratory for New Resources&Quality Evaluation of Chinese Medicine Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Second Affiliated Hospital of Navy Medical UniversityShanghai 200003China Kunming Plant Classification Biotechnology Co. Ltd.Kunming 650500China School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Products Kunming Medical UniversityKunming 650500China State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs Beijing100700China

The aerial parts of Mosla chinensis *** Mosla chinensis cv.'Jiangxiangru'(MCJ)are widely utilized in traditional Chinese medicine(TCM),known collectively as ***,due to clinical effectiveness concerns and frequent misidentification,the original plants have increasingly been substituted by various species within the genera Elsholtzia and *** challenge in distinguishing between these genera arises from their similar morphological and metabolic *** address this issue,our study introduced a rapid method for metabolic characterization,employing high-resolution mass spectrometry-based *** detailed biosynthetic and chemometric analyses,we pinpointed five phenolic compounds—salviaflaside,cynaroside,scutellarein-7-O-D-glucoside,rutin,and vicenin-2—among 203 identified compounds,as reliable chemical markers for distinguishing Xiang-ru from closely related Elsholtzia *** methodology holds promise for broad application in the analysis of plant aerial parts,especially in verifying the authenticity of aromatic traditional medicinal *** findings underscore the importance of non-volatile compounds as dependable chemical markers in the authentication process of aromatic traditional medicinal plants.

关键词： Genus Elsholtzia Genus Mosla Traditional Chinese medicine Xiang-ru Non-volatile phenolic compounds Liquid chromatography-mass spectrometry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

芒果苷的药效作用研究进展

引用

海军军医大学学报 2024年第2期45卷 219-226页

作者：范香成焦广洋张凤韩军陈万生海军军医大学(第二军医大学)第二附属医院药剂科上海200003 上海市药物(中药)代谢产物研究重点实验室上海200433 上海中医药大学中药研究所上海201203 海军军医大学(第二军医大学)第二附属医院消化内科上海200003

芒果苷是一种天然存在的C-葡萄糖基黄酮,是有效的药用化合物,可以从多种植物中分离提取。研究表明,芒果苷具有治疗糖尿病、抗肿瘤、抗炎、抗氧化、治疗眼部疾病、神经系统保护、肾脏保护、改善心血管疾病等作用。其具有众多的生物活性,... 详细信息

芒果苷是一种天然存在的C-葡萄糖基黄酮,是有效的药用化合物,可以从多种植物中分离提取。研究表明,芒果苷具有治疗糖尿病、抗肿瘤、抗炎、抗氧化、治疗眼部疾病、神经系统保护、肾脏保护、改善心血管疾病等作用。其具有众多的生物活性,对预防和治疗多种疾病有巨大潜力。本文对近年来芒果苷的药效学研究进展及其作用机制进行综述,以期为芒果苷的进一步研究和利用提供科学依据。

关键词：芒果苷黄酮药效学糖尿病肿瘤作用机制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

代谢组学在肺癌治疗生物标志物中的研究进展

引用

中国医院药学杂志 2024年第3期44卷 356-359页

作者：邓易汤浩李明明张凤陈万生上海市松江区中心医院药剂科上海310000 海军军医大学第二附属医院药剂科上海200003 上海中医药大学中药研究所上海201203

肺癌具有死亡率高、预后差、5年生存率低的特点,目前以化疗为主的综合治疗方案是非小细胞肺癌(non-small cell lung cancer,NSCLC)的主要治疗方式。根据肺癌的分期分型,患者相应接受包括手术、放疗、化疗以及靶向的综合治疗,然而早期症... 详细信息

肺癌具有死亡率高、预后差、5年生存率低的特点,目前以化疗为主的综合治疗方案是非小细胞肺癌(non-small cell lung cancer,NSCLC)的主要治疗方式。根据肺癌的分期分型,患者相应接受包括手术、放疗、化疗以及靶向的综合治疗,然而早期症状不显著、个体化差异巨大、放疗抵抗和化疗耐药等问题限制了患者的诊断和治疗效果,从而严重影响患者的身体健康。代谢组学从小分子代谢物的角度为发掘肿瘤生物标志物、评估整体代谢变化、研究发病机制提供着越来越重要的作用。该文主要综述了基于代谢组学技术的肺癌治疗预后效果评价生物标志物的研究进展,以期为肺癌个体化精准治疗提供重要理论依据,帮助临床患者取得治疗收益的最大化。

关键词：代谢组学生物标志物肺癌预后效果

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中药品质调控与资源可持续利用的思考与方法探讨

引用

上海中医药大学学报 2024年第1期38卷 8-17页

作者：肖莹邱实陈瑞兵管芹陈万生上海中医药大学中药研究所中药新资源与品质评价国家中医药管理局重点研究室中药标准化教育部重点实验室上海市复方中药重点实验室上海201203 海军军医大学药学院上海200433

高品质中药资源是中医药全产业链健康发展的物质载体和战略基石。当前,大宗药材品质退化、珍稀濒危资源匮乏,优质中药资源需求和供给矛盾日益突出,成为制约中医药高质量发展的瓶颈问题。作者团队长期从事中药品质调控与资源可持续利用... 详细信息

高品质中药资源是中医药全产业链健康发展的物质载体和战略基石。当前,大宗药材品质退化、珍稀濒危资源匮乏,优质中药资源需求和供给矛盾日益突出,成为制约中医药高质量发展的瓶颈问题。作者团队长期从事中药品质调控与资源可持续利用研究。该文结合研究实例,从“道地药材品质形成机制研究、中药活性成分生源途径解析、中药材优良品种的培育、中药活性成分异源生物制造”几个方面阐述中药品质调控与资源可持续利用的思路和方法,为本领域相关研究提供参考和借鉴。

关键词：中药资源中药品质品质调控资源可持续利用

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

细胞色素P450酶在植物次生代谢产物生物合成中的研究进展

引用

分子植物育种 2024年第7期22卷 2176-2183页

作者：李永康马雪祺冯婧娴陈万生孙连娜肖莹上海中医药大学中药研究所中药资源与生物技术中心中药炮制教研室上海201203 海军军医大学附属长征医院医疗保障中心上海200003

细胞色素P450 (cytochrome P450, CYP450)是一类广泛存在于自然界的超基因家族单加氧酶,参与多种植物次生代谢产物的生物合成,被誉为“万能的生物催化剂”。近年来,随着高通量测序技术和分子生物学的发展,越来越多植物CYP450酶的功能被... 详细信息

细胞色素P450 (cytochrome P450, CYP450)是一类广泛存在于自然界的超基因家族单加氧酶,参与多种植物次生代谢产物的生物合成,被誉为“万能的生物催化剂”。近年来,随着高通量测序技术和分子生物学的发展,越来越多植物CYP450酶的功能被揭示,相关生物合成研究取得了令人瞩目的成果。本研究结合CYP450的结构特点、作用机制,重点介绍植物CYP450在次生代谢产物生物合成中的研究进展,为植物CYP450酶的挖掘及功能研究提供参考,也为基于此的植物次生代谢产物生物合成与代谢调控提供依据。

关键词：细胞色素P450酶植物次生代谢产物生物合成蛋白结构

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phenotyping of Salvia miltiorrhiza Roots Reveals Associations between Root Traits and Bioactive Components

引用

Plant Phenomics 2023年第4期5卷 746-756页

作者： Junfeng chen Yun Wang Peng Di Yulong Wu Shi Qiu Zongyou Lv Yuqi Qiao Yajing Li Jingfu Tan Weixu chen Ma Yu Ping Wei Ying Xiao wansheng chen The SATCM Key Laboratory for New Resources&Quality Evaluation of Chinese Medicine Institute of Chinese Materia MedicaShanghai University of Traditional Chinese MedicineShanghai 201203China School of Medicine Shanghai UniversityShanghai 200444China State Local Joint Engineering Research Center of Ginseng Breeding and Application Jilin Agricultural UniversityChangchun 130118China School of Computer Science Sichuan Normal UniversityChengdu 610066China Shangyao Huayu(Linyi)Traditional Chinese Resources Co. Ltd.Linyi 276000China School of Life Science and Engineering Southwest University of Science and TechnologyMianyang 621010SichuanChina Sichuan Academy of Traditional Chinese Medicine Chengdu 610041China Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003China

Plant phenomics aims to perform high-throughput,rapid,and accurate measurement of plant traits,facilitating the identification of desirable traits and optimal genotypes for crop *** miltiorrhiza(Danshen)roots possess remarkable therapeutic effect on cardiovascular diseases,with huge market *** great advances have been made in metabolic studies of the bioactive metabolites,investigation for *** roots on other physiological aspects is ***,we developed a framework that utilizes image feature extraction software for in-depth phenotyping of *** *** employing multiple software programs,*** roots were described from 3 aspects:agronomic traits,anatomy traits,and root system *** K-means clustering based on the diameter ranges of each root branch,all roots were categorized into 3 groups,with primary root-associated key *** a proof of concept,we examined the phenotypic components in a series of randomly collected *** roots,demonstrating that the total surface of root was the best parameter for the biomass prediction with high linear regression correlation(R^(2)=0.8312),which was sufficient for subsequently estimating the production of bioactive metabolites without content *** study provides an important approach for further grading of medicinal materials and breeding practices.

关键词： Root utilize anatomy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The transcription factors TLR1 and TLR2 negatively regulate trichome density and artemisinin levels in Artemisia annua

引用

Journal of Integrative Plant Biology 2022年第6期64卷 1212-1228页

作者： Zongyou Lv JinXing Li Shi Qiu Fei Qi Hang Su Qitao Bu Rui Jiang Kexuan Tang Lei Zhang wansheng chen Research and Development Center of Chinese Medicine Resources and Biotechnology Shanghai University of Traditional Chinese MedicineShanghai 201203China Department of Pharmacy Changzheng HospitalSecond Military Medical UniversityShanghai 200003China Department of Pharmaceutical Botany School of PharmacySecond Military Medical UniversityShanghai 200433China Joint International Research Laboratory of Metabolic&Developmental Sciences Key Laboratory of Urban Agriculture(South)Ministry of AgriculturePlant Biotechnology Research CenterFudan‐SJTU‐Nottingham Plant Biotechnology R&D CenterShanghai Jiao Tong UniversityShanghai 200240China State Key Laboratory of Subtropical Silviculture Zhejiang A&F UniversityHangzhou 311300China

The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes and the artemisinin content correlates with trichome density;however,the factors affecting trichome development are largely ***,we demonstrate that the *** R2R3 MYB transcription factor TrichomeLess Regulator 1(TLR1)negatively regulates trichome *** ***,TLR1 overexpression lines had 44.7%–64.0%lower trichome density and 11.5%–49.4%lower artemisinin contents and TLR1-RNAi lines had 33%–93.3%higher trichome density and 32.2%–84.0%higher artemisinin contents compared with non-transgenic ***1 also negatively regulates the expression of anthocyanin biosynthetic pathway genes in *** heterologously expressed in Arabidopsis thaliana,TLR1 interacts with GLABROUS3a,positive regulator of trichome development,and represses trichome *** two-hybrid and pull-down assays indicated that TLR1 interacts with the WUSCHEL homeobox(WOX)protein AaWOX1,which interacts with the LEAFY-like transcription factor ***2 overexpression in Arabidopsis and *** showed that TLR2 reduces trichome development by reducing gibberellin ***,artemisinin contents were 19%–43%lower in TLR2-overexpressing *** plants compared to *** data indicate that TLR1 and TLR2 negatively regulate trichome density by lowering gibberellin levels and may enable approaches to enhance artemisinin yields.

关键词： artemisinin Artemisia annua L. LFY MYB transcription factor trichome WOX

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于“辨状论质”的地域性市售丹参饮片质量等级评价研究

引用

上海中医药大学学报 2024年第1期38卷 18-26页

作者：冯昊陈俊源黄豆豆杨颖博肖莹陈万生孙连娜上海中医药大学中药学院上海201203 上海中医药大学中药研究所上海201203 江苏康缘药业股份有限公司江苏连云港222001

目的:考察丹参饮片性状指标与内在成分含量的相关性,筛选获得可行可控的质控指标,建立科学合理的地域性市售丹参饮片等级标准。方法:基于“辨状论质”理论,将来源于上海市售的不同直径和不同切面颜色丹参饮片分等,采用色差仪对不同切面... 详细信息

目的:考察丹参饮片性状指标与内在成分含量的相关性,筛选获得可行可控的质控指标,建立科学合理的地域性市售丹参饮片等级标准。方法:基于“辨状论质”理论,将来源于上海市售的不同直径和不同切面颜色丹参饮片分等,采用色差仪对不同切面颜色丹参饮片粉末颜色值进行测量,结合饮片指标性化学成分含量,使用OriginLab 2021软件进行Pearson相关性分析。结果:丹参饮片直径与总丹参酮和丹参酮ⅡA含量呈极显著负相关,与丹酚酸含量呈极显著正相关;丹参饮片切面颜色与粉末L值呈极显著负相关。丹参饮片粉末L值与丹酚酸B含量呈极显著正相关,b值与丹酚酸B含量呈极显著负相关。结论:丹参饮片直径和切面颜色指标可作为其内在品质判断指标,为上海地区市售丹参饮片等级标准提供客观、科学的理论依据。

关键词：丹参饮片直径切面颜色相关性分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Increased Vitamin C Content Accompanied by an Enhanced Recycling Pathway Confers Oxidative Stress Tolerance in Arabidopsis

引用

Journal of Integrative Plant Biology 2010年第4期52卷 400-409页

作者： zinan wang Ying Xiao wansheng chen Kexuan Tang Lei Zhang State Key Laboratory of Genetic Engineering School of Life Sciences Fudan-SJTU-Nottingham Plant Biotechnology R&D Center Fudan University Shanghai 200433 China Department of Pharmacognosy School of Pharmacy Second Military Medical University Shanghai 200433 China Department of Pharmacy Changzheng Hospital second Military Medical University Shanghai 200003 China

Vitamin C （L-ascorbic acid, AsA） has important antioxidant and metabolic functions in both plants and animals. Once used, ascorbic acid can be regenerated from its oxidized form in a reaction catalyzed by dehydroascorbate reductase （DHAR, EC 1.***.5.1）. To analyze the physiological role of DHAR catalyzing the reduction of DHA to ascorbate in environmental stress adaptation, we examined whether increasing the level of AsA through enhanced AsA recycling would limit the deleterious effects of oxidative stress. A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-dhar gene was introduced into Arabidopsis thaliana. Transgenic plants were biochemically characterized and tested for responses to oxidative stress. Western blot indicated that the dhar-transgene was successfully expressed. In homozygous T4 transgenic seedlings, DHAR overexpression was increased up to 1.5 to 5.4 fold, which enhanced foliar ascorbic acid levels 2- to 4.25-fold and ratio of AsA/DHA about 3- to 16-fold relative to wild type. In addition, the level of glutathione, the reductant used by DHAR, also increased as did its redox state. When whole plants were treated with high light and high temperature stress or in vitro leaf discs were subjected to 10μM paraquat, transgenic plants showed a larger AsA pool size, lower membrane damage, and a higher level of chlorophyll compared with controls. These data suggested that increasing the plant vitamin C content through enhanced ascorbate recycling could limit the deleterious effects of environmental oxidative stress.

关键词： Stress Tolerance Increased Vitamin Enhanced Recycling

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：