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Interannual evolution of the chemical composition,sources and processes of PM_(2.5)in chengdu,china:Insights from observations in four winters

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Journal of Environmental sciences 2024年第4期138卷 32-45页

作者： Junke Zhang Jiaqi Li Yunfei Su Chunying chen Luyao chen Xiaojuan Huang Fangzheng Wang Yawen Huang Gehui Wang Faculty of Geosciences and Environmental Engineering Southwest Jiaotong UniversityChengdu 611756China Department of Environmental Science&Engineering Shanghai Key Laboratory of Atmospheric Particle Pollution and Prevention(LAP3)Fudan UniversityShanghai 200438China Key Lab of Geographic Information Science of the Ministry of Education School of Geographic SciencesEast China Normal UniversityShanghai 200241China

The air quality in china has improved significantly in the last decade and,correspondingly,the characteristics of PM_(2.5)have also *** studied the interannual variation of PM_(2.5)in chengdu,one of the most heavily polluted megacities in southwest china,during the most polluted season(winter).Our results show that the mass concentrations of PM_(2.5)decreased significantly year-by-year,from 195.8±91.0μg/m~3in winter 2016 to 96.1±39.3μg/m^(3)in winter *** mass concentrations of organic matter(OM),SO_()4^(2-),NH_(4)^(+)and NO_(3)^(-)decreased by 49.6%,57.1%,49.7% and 28.7%,*** differential reduction in the concentrations of chemical components increased the contributions from secondary organic carbon and NO_(3)^(-)and there was a larger contribution from mobile *** contribution of OM and NO_(3)^(-)not only increased with increasing levels of pollution,but also increased year-by-year at the same level of *** sources of PM_(2.5)were identified:combustion sources,vehicular emissions,dust and secondary *** aerosols made the highest contribution and increased year-by-year,from 40.6%in winter 2016 to 46.3% in winter *** contrast,the contribution from combustion sources decreased from 14.4% to 8.7%.Our results show the effectiveness of earlier pollution reduction policies and emphasizes that priority should be given to key pollutants(e.g.,OM and NO_(3)^(-))and sources(secondary aerosols and vehicular emissions)in future policies for the reduction of pollution in chengdu during the winter months.

关键词： PM_(2.5) Chemical composition Sources Interannual evolution Winter chengdu

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循环逆转录反应方法及应用条件研究

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生物化学与生物物理进展 2001年第4期28卷 560-562页

作者：陈燃金詟伍迪唐榕毛裕民复旦大学生命科学学院遗传所遗传工程国家重点实验室上海200433

建立了循环逆转录反应方法 (repeatedreversetranscriptionreaction ,RRTR) .其原理是通过控制高温变性低温退火延伸的循环过程 ,反复利用起始RNA模板进行逆转录反应 .RNA杂交的结果证明在RRTR过程中 ,RNA模板是保持稳定的 ;点杂交结... 详细信息

建立了循环逆转录反应方法 (repeatedreversetranscriptionreaction ,RRTR) .其原理是通过控制高温变性低温退火延伸的循环过程 ,反复利用起始RNA模板进行逆转录反应 .RNA杂交的结果证明在RRTR过程中 ,RNA模板是保持稳定的 ;点杂交结果表明cDNA产物量得到了增加 ;定量PCR的结果表明RRTR是cDNA线性增长过程 .结果提示 ,RRTR可有效地提高RNA检测灵敏度。

关键词：循环逆转录反应 RNA 定量分析应用

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5种药用芦荟中芦荟甙含量的测定

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西南师范大学学报（自然科学版） 2001年第6期26卷 709-711页

作者：廖志华陈敏谈锋复旦大学遗传学研究所上海200433 复旦大学药学院上海200433 西南师范大学生命科学学院重庆400715

对库拉索芦荟、木立芦荟、好望角芦荟、中华芦荟和皂质芦荟 5种药用芦荟中芦荟甙的含量进行了定量的测定 ,发现全叶干粉中芦荟甙含量差异显著 ,含量高低依次为 :库拉索芦荟、木立芦荟、好望角芦荟、中华芦荟、皂质芦荟 ;而以鲜质量计算... 详细信息

对库拉索芦荟、木立芦荟、好望角芦荟、中华芦荟和皂质芦荟 5种药用芦荟中芦荟甙的含量进行了定量的测定 ,发现全叶干粉中芦荟甙含量差异显著 ,含量高低依次为 :库拉索芦荟、木立芦荟、好望角芦荟、中华芦荟、皂质芦荟 ;而以鲜质量计算芦荟甙含量 ,发现库拉索芦荟、木立芦荟和好望角芦荟中芦荟甙含量接近。

关键词：库拉索芦荟木立芦荟好望角芦荟中华芦荟皂质芦荟芦荟甙含量测定药用芦荟

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Spatial impact of digital finance on carbon productivity

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Geoscience Frontiers 2024年第3期15卷 358-372页

作者： Huaping Sun Tingting chen Christoph Nedopil Wang school of Finance and Economics Jiangsu UniversityZhenjiangChina school of Economics and Management University of Science and Technology BeijingBeijingChina Fanhai International school of Finance Fudan UniversityShanghaiChina

Low carbon productivity has been identified as a key direction for china’s future *** an important driving force for economic growth,the question of whether digital finance that is reliant on digital technology can support the development of a low-carbon urban economy remains *** on the carbon productivity measured by panel data from 201 cities for the period 2011-2020,this study applies the spatial Dubin model and threshold regression model to explore the impact of digital finance on carbon productivity,yielding the following key ***,the spatial distribution heterogeneity of carbon productivity in china’s eastern region is higher than that in the western region,and both productivity and digital finance are characterized by high(low)-high(low)dotted spatial ***,digital finance can significantly improve carbon productivity via two transmission channels:the human capital and marketization *** the same time,digital finance exerts a spatial spillover effect on carbon productivity,and rising local digital finance levels will increase carbon productivity in neighboring *** analysis indicates that the spillover effect of digital finance in urban agglomerations and eastern regions is more ***,fixed-asset investment has a positive nonlinear moderating effect on digital finance,thus improving carbon *** the per capita investment in fixed assets does not exceed 682.73 yuan,digital finance exerts only a limit pulling effect on carbon productivity;when it is higher than this value,the pulling effect is intensified.

关键词： Digital finance Carbon productivity Spatial spillover effect Fixed-asset investment Spatial heterogeneity

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复旦大学大学生吸烟情况及影响因素调查

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复旦学报（医学版） 2003年第5期30卷 487-490页

作者：陈静孙亮钱海红傅东波郑频频傅华复旦大学公共卫生学院预防医学教研室上海200032 复旦大学团委上海200433

目的　了解复旦大学大学生吸烟现状 ,分析该人群吸烟行为的影响因素 ,寻找有效的控烟策略以预防和矫正大学生的吸烟行为。方法　对 4 0 19名在校本科生进行了问卷调查。结果　吸烟率为 2 .3% ,现在吸烟率为 2 .3%。性别、年级、专业与... 详细信息

目的　了解复旦大学大学生吸烟现状 ,分析该人群吸烟行为的影响因素 ,寻找有效的控烟策略以预防和矫正大学生的吸烟行为。方法　对 4 0 19名在校本科生进行了问卷调查。结果　吸烟率为 2 .3% ,现在吸烟率为 2 .3%。性别、年级、专业与现在是否吸烟有关。男性是吸烟的危险因素 ;随着年级的升高 ,吸烟率呈上升趋势 ;非医学专业的吸烟率高于医学专业。结论　大学生对吸烟的态度和信念较好 ,但是吸烟率仍然处于一个比较高的水平 ,近一半的吸烟者并没有戒烟的打算 ,非吸烟者拒绝被动吸烟的意识也比较薄弱。开展控烟运动已刻不容缓 ,成为当务之急。

关键词：复旦大学大学生吸烟情况影响因素调查控烟策略预防矫正

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FHC和Bim相互作用抵抗细胞凋亡(英文)

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生物化学与生物物理进展 2011年第9期38卷 859-865页

作者：邹冠玉季朝能沈琪薛京伦陈金中复旦大学生命科学学院遗传工程国家重点实验室上海200433

FHC和Bim参与细胞铁代谢和由ROS引起的细胞凋亡过程.但是其具体的分子机制还未阐明.用pLexA-Bim L作为诱饵,筛选了一个基于pBD42AD的胎脑cDNA文库,发现FHC是一个新的Bim相互作用蛋白.酵母杂交实验发现Bim的相互作用片段为BH3功能域.上... 详细信息

FHC和Bim参与细胞铁代谢和由ROS引起的细胞凋亡过程.但是其具体的分子机制还未阐明.用pLexA-Bim L作为诱饵,筛选了一个基于pBD42AD的胎脑cDNA文库,发现FHC是一个新的Bim相互作用蛋白.酵母杂交实验发现Bim的相互作用片段为BH3功能域.上述相互作用进一步用免疫共沉淀和荧光共定位得以证实.在HEK293细胞过表达FHC可以减轻由Bim过表达或ROS所引起的细胞凋亡,而用FHC特异性siRNA调低FHC表达,则增加Bim过表达或ROS引起的细胞凋亡.研究首次报道了Bim和FHC的相互作用以及对细胞凋亡和氧化应激的影响,为进一步阐明FHC和Bim参与凋亡和ROS反应提供了新的线索.

关键词：铁蛋白重链FHC Bim 氧化应激细胞凋亡

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Costunolide alleviates HKSA-induced acute lung injury via inhibition of macrophage activation

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Acta Pharmacologica Sinica 2019年第8期40卷 1040-1048页

作者： Yun-tian chen Yao Du Bo Zhao Li-xing Gan Kai-kai Yu Lei Sun Jian Wang Feng Qian Department of Respiratory Medicine Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of Medicine Department of Infectious Diseases The Fifth People’s Hospital of ShanghaiFudan University Engineering Research Center of Cell & Therapeutic Antibody Ministry of EducationSchool of PharmacyShanghai Jiao Tong University

Staphylococcus aureus(***)infection leads to a severe inflammatory response and causes acute lung injury(ALI),eventually threatening human ***,it is of importance to find an agent to inhibit inflammation and reduce ***,we found that costunolide,a sesquiterpene lactone,displays anti-inflammatory effects and ameliorates heat-killed ***(HKSA)-induced *** treatment attenuated HKSA-induced murine ALI in which pulmonary neutrophil infiltration was inhibited,lung edema was decreased,and the production of pro-inflammatory cytokines was significantly *** addition,costunolide dose-dependently inhibited the generation of IL-6,TNF-α,IL-1β,and keratinocyte-derived cytokine(KC),as well as the expression of iNOS,in HKSA-induced ***,costunolide attenuated the phosphorylation of p38 MAPK and cAMP response element-binding protein(CREB).Collectively,our findings suggested that costunolide is a promising agent for alleviating bacterialinduced ALI via the inhibition of the MAPK signaling pathways.

关键词： Costunolide Staphylococcus aureus pneumonia macrophage MAPK signaling

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违约精神损害赔偿刍议

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天津大学学报（社会科学版） 2004年第4期6卷 355-359页

作者：陈晨复旦大学法学院上海200433

通过对最高人民法院《关于确定民事侵权精神损害赔偿责任若干问题的解释》中违反合同不得请求精神损害赔偿的规定的研究以及对当今各国违约精神损害赔偿立法的考察,论证了违约精神损害赔偿的必要性与可能性,并对我国违约精神损害赔偿制... 详细信息

通过对最高人民法院《关于确定民事侵权精神损害赔偿责任若干问题的解释》中违反合同不得请求精神损害赔偿的规定的研究以及对当今各国违约精神损害赔偿立法的考察,论证了违约精神损害赔偿的必要性与可能性,并对我国违约精神损害赔偿制度的设计提出了初步设想。

关键词：违约精神损害赔偿特殊类型合同可预见性

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Blastocyst Formation and Chromosome Statuses of Reconstructed Embryos Derived from Interspecies Somatic Cell Nuclear Transfer (iSCNT)

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Journal of Reproduction and Contraception 2013年第1期24卷 38-44页

作者： Xiao-hong GUAN Yun FENG Xiao-wei LU Jian-quan chen Yi-juan SUN Hong-ying SHA Reproductive Medical Center Ruijin Hospital Medical School of Shanghai Jiaotong University shanghai Transgenic Research Center Department of Neurosurgery Fudan University Huashan Hospital National Key Laboratory for Medical Neurobiology Fudan University

Objective To analyze the blastocyst formation and chromosome statuses of reconstructed embryos derived from human-goat interspecies somatic cell nuclear transfer （iSCNT）, exploring the development retardant factors. Methods Human specific point probes cep2, cep6, tel2 and 13q14.2, 21q22.13 combining fluorescence in-situ hybridization （FISH） technology were used to test trophectoderm cells of blastocyst and blastomeres of development arrest nuclear transfer （NT） embryos. Results A total of 209 reconstructed embryos were recovered, and the rate of blastocyst formation was 3.8% （8/209）. FISH signals showed that chromosomal abnormalities were present in 2 blastocysts （2/8） and 146 development arrest embryos （146/201）. Conclusion The rate of blastocyst formation is low, and reconstituted embryos of development arrest showed extensive chromosome abnormalities, suggesting that a chromosomal mechanism may underlie their developmental arrest.

关键词： interspecies somatic cell nuclear transfer (iSCNT) preimplantation embryonicdevelopment fluorescence in-situ hybridization (FISH) chromosomal status

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Functional expression of Vitreoscilla hemoglobin(VHb)in Arabidopsis relieves submergence,nitrosative,photo-oxidative stress and enhances antioxidants metabolism

Functional expression of Vitreoscilla hemoglobin(VHb)in Arab...

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中华中医药学会第九届中药鉴定学术会议

作者： Lei Zhang~1,Zinan Wang~2,Xiayan Mai~3,Wangsheng chen~4,Kexuan Tang~2 1.Department of pharmacognosy,school of Pharmacy,Second Military Medical university, shanghai 200433,china 2.State Key Laboratory of Genetic Engineering,school of Life sciences,fudan university, shanghai 200433,china 3.shanghai Institute of Plant Physiology and Ecology,The Chinese Academy of sciences, shanghai,china 4.Department of Pharmacy,Changzheng Hospital,Second Military Medical university, shanghai 200003,china.

A chimeric construct consisting of the double CaMV35S promoter fused to the Myc-vhb gene encoding Vitreoscilla (bacterial) hemoglobin(VHb) was introduced into Arabidopsis thaliana and transgenic plants were cultured under various growth and stress conditions with the aim of studying the generality *** analysis indicated that the exogenous vhb was stably integrated into the Arabidopsis genome and successfully *** vhb expression improved the germination rate of transgenic *** submergence conditions,in vitro and in vivo bioassays revealed that the vhb expression protected transgenic plants against prolonged *** discs from the transgenic plants maintained in standard growth conditions reveal increased tolerance to nitrosative stress by sodium nitroprusside(SNP) *** differences in chlorophyll a,b,total protein content and conductivity contents were observed among the transgenic,wild-type(WT) and blank transformation(CK) *** significant differences were observed between the tested VHb,WT and CK lines in the presence of oxidative stresses by paraquat *** expression also affected the levels of some Arabidopsis endogenesis genes that are involved in oxygen metabolism and antioxidants biosynthesis;up-regulating their expression at transcriptional ***-MS revealed that VHb expression shifted plant secondary metabolism,which may reflect the availability of extra metabolite production for possible enhanced protection *** transgenic plants were more tolerant to photo-oxidative damage due to the increased antioxidants,such as *** results suggested that vhb gene might have the potential use in molecular breeding for the improvement of stress resistance and fine chemicals production in plants.

关键词： Vitreoscilla hemoglobin Arabidopsis thaliana life-cycle flooding nitrosative stress oxidative stress secondary metabolism profile antioxidant

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