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Quantifying cell binding kinetics mediated by surface-bound blood type B antigen to immobilized antibodies

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Chinese Science Bulletin 2008年第23期53卷 3634-3641页

作者： LI BaoXia chen Juan LONG mian National Microgravity Laboratory and Center of Biomechanics and Bioengineering Institute of Mechanics Chinese Academy ofSciences Beijing 100190 China

Cell adhesion is crucial to many biological processes, such as inflammatory responses, tumor metastasis and thrombosis formation. Recently a commercial surface plasmon resonance (SPR)-based BIAcore biosensor has been extended to determine cell binding mediated by surface-bound bio-molecular interactions. How such cell binding is quantitatively governed by kinetic rates and regulating factors, however, has been poorly understood. Here we developed a novel assay to determine the binding kinetics of surface-bound biomolecular interactions using a commercial BIAcore 3000 bio-sensor. Human red blood cells (RBCs) presenting blood group B antigen and CM5 chip bearing immobilized anti-B monoclonal antibody (mAb) were used to obtain the time courses of response unit, or sensorgrams, when flowing RBCs over the chip surface. A cellular kinetic model was proposed to correlate the sensorgrams with kinetic rates. Impacts of regulating factors, such as cell concentration, flow duration and rate, antibody-presenting level, as well as pH value and osmotic pressure of suspending medium were tested systematically, which imparted the confidence that the approach can be applied to kinetic measurements of cell adhesion mediated by surface-bound biomolecular interactions. These results provided a new insight into quantifying cell binding using a commercial SPR-based BIAcore biosensor.

关键词：生物传感器细胞动力模式细胞学构成模式

Deformation Mechanisms and Safe Drilling Fluids Density in Extremely Thick Salt Formations

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Petroleum Science 2007年第4期4卷 56-61页

作者： Yang Henglin chen mian Zhang Guangqing Drilling Research Institute CNPC Beijing 100097 China School of Petroleum Engineering China University of Petroleum Beijing 102249 China

Hydrocarbons are very often associated with salt structures. The oil and gas industry is often required to drill along and through long salt sections to reach and recover hydrocarbons. The unique physical properties of salt require special techniques to ensure borehole stability and adequate casing design. This paper assumed that the mechanical behavior of salt is regulated by the magnitude of mean stress and octahedral shear stress and under the influence of different stress conditions the deformation of rock salt can be represented by three domains, i.e. compression domain, volume unchanged domain, and dilatancy domain, which are separated by a stress dependent boundary. In the compression domain, the volume of salt decreases until all microcracks are closed, with only elastic deformation and pure creep; in the volume unchanged domain the deformation is considered steady incompressible flow controlled by pure creep; and in the dilatancy domain the volume of salt increases during deformation due to micro-cracking, causing damage and accelerating ＂creep＂ until failure. This paper presents a hypothesis that the borehole is stable only when the magnitude of octahedral shear stress is below the dilatancy boundary. It gives the design method for determining drilling fluids density, and calculates the closure rate ofborehole with the recommended drilling fluids density. If the closure rate of the borehole is less than 0.1%, the drilling fluids density window can be used during drilling through extremely thick salt formations.

关键词： Rock salt borehole stability deformation mechanisms constitutive equations equivalent viscosity drilling fluids density

An adaptive physics-informed deep learning method for pore pressure prediction using seismic data

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Petroleum Science 2024年第2期21卷 885-902页

作者： Xin Zhang Yun-Hu Lu Yan Jin mian chen Bo Zhou State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum(Beijing)Beijing102249China College of Petroleum Engineering China University of Petroleum(Beijing)Beijing102249China College of Artificial Intelligence China University of Petroleum(Beijing)Beijing102249China PetroChina Tarim Oilfield Company KorlaXinjiang841000China

Accurate prediction of formation pore pressure is essential to predict fluid flow and manage hydrocarbon production in petroleum *** deep learning technique has been receiving more interest due to the great potential to deal with pore pressure ***,most of the traditional deep learning models are less efficient to address generalization *** fill this technical gap,in this work,we developed a new adaptive physics-informed deep learning model with high generalization capability to predict pore pressure values directly from seismic ***,the new model,named CGP-NN,consists of a novel parametric features extraction approach(1DCPP),a stacked multilayer gated recurrent model(multilayer GRU),and an adaptive physics-informed loss *** machine training,the developed model can automatically select the optimal physical model to constrain the results for each pore pressure *** CGP-NN model has the best generalization when the physicsrelated metricλ=0.5.A hybrid approach combining Eaton and Bowers methods is also proposed to build machine-learnable labels for solving the problem of few *** validate the developed model and methodology,a case study on a complex reservoir in Tarim Basin was further performed to demonstrate the high accuracy on the pore pressure prediction of new wells along with the strong generalization *** adaptive physics-informed deep learning approach presented here has potential application in the prediction of pore pressures coupled with multiple genesis mechanisms using seismic data.

关键词： Pore pressure prediction Seismic data 1D convolution pyramid pooling Adaptive physics-informed loss function High generalization capability

Effective stress law for anisotropic double porous media

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Chinese Science Bulletin 2004年第21期49卷 2327-2331页

作者： ZHAO Ying chen mian ZHANG Guangqing School of Oil&Gas Engineering University of PetroleumBeijing102249China

An effective stress law is derived analytically to describe the effect of pore (fracture pore and matrix-block pore) fluid pressure on the linearly elastic response of ani- sotropic saturated dual-porous rocks, which exhibit anisot- ropy. For general anisotropy the difference between the ef- fective stress and the applied stress is not hydrostatic simply multiplied by Biot coefficient. The effective stress law in- volves four constants for transversely isotropic response; these constants can be expressed in terms of the moduli of the single porous material, double porous material and of the solid material. These expressions are simplified considerably when the anisotropy is structural rather than intrinsic, i.e. in the case of an isotropic solid material with an anisotropic pore structure. In this case the effective stress law involves grain bulk modulus, four moduli and two compliances of the porous material for transverse isotropy. The law reduces, in the case of isotropic response, to that suggested by Li Shuiquan (2001). And reduction to the single-porosity (de- rived analytically by Carroll (1979)) is presented to demon- strate the conceptual consistency of the proposed law.

关键词： anisotropy single porous media double porous media pore fracture effective stress

Numerical stress analysis for the multi-casing structure inside a wellbore in the formation using the boundary element method

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Petroleum Science 2017年第1期14卷 126-137页

作者： Wan cheng Yan Jin mian chen Guo-Sheng Jiang State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum Faculty of Engineering China University of Geosciences

A multi-casing structure in drilling engineering can be considered as an inhomogeneous body consisting of many different materials. The mechanical behavior of the inhomogeneous body in an infinite domain is very com- plicated. In this paper, a detailed expression about the fictitious stress method of the boundary element method （BEM） is demonstrated for the inhomogeneous body. Then the fictitious stress method is deployed to investigate the stresses for the multi-casing structure under non-uniform loading conditions and an irregular wellbore. Three examples of the multi-casing structure in the borehole imply the high effectiveness of BEM for complex geometries related to the borehole in an infinite formation. The effects of casing eccentricity and the interfacial gap on the stress field are discussed. The eccentric casing takes the potential yield when the eccentric orientation is along the direction of Sh. Under different eccentric orientations, the yon Mises stress in the casing increases with increasing degree of eccentricity. The radial stress in the multi-casing structure is always continuous along the radius, but the circumferential stress is discontinuous at the interface. The radial stress decreases and the circumferential stress increases with the increasing of the interfacial gap between the adjacent materials.

关键词： Displacement discontinuity method Fictitiousstress method Drilling mechanics Wellbore stressconcentration Inhomogeneous body

Hydraulic fracture initiation theory for a horizontal well in a coal seam

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Petroleum Science 2013年第2期10卷 219-225页

作者： Hou Bing chen mian Wang Zheng Yuan Jianbo Liu Ming State Key Laboratory of Petroleum Resource and Prospecting China University of Petroleum Security Department Tuha Oil Company

A series of experiments were pertbrmed to determine rock mechanical parameters related to hydraulic fracturing of coal. The effect of confining pressure and pore pressure on the strength of coal was stt, died. Experimental results show that the coal seam in the study areas has a relatively low elastic modulus, high Poisson＇s ratio, high fragility and is easily broken and compressed. The coal seam is considered as a transversely isotropic medium, since the physical properties in the direction of bedding plane and orthogonal to the bedding plane vary markedly. Based on the generalized plane strain model, stress distribution for an arbitrarily orientated wellbore in the coal seam was determined. In a horizontal well, hydraulic fracturing was lbund to initiate in the coal seam mass due to tensile failure, or from cleats due to shear or tensile failure. For those coal seams with abundant natural cleats, hydraulic fracture initiation can be induced by any of these mechanisms. In this study, hydraulic fracture initiation criteria tbr a horizontal well in a coal seam were established.

关键词： Hydraulic fracturing coal seam transversely isotropic fracture initiation cleat naturalfracture

Antibacterial ability and biocompatibility of fluorinated titanium by plasma-based surface modification

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Rare Metals 2022年第2期41卷 689-699页

作者： mian chen Xiao-Qiao Wang Er-Lin Zhang Yi-Zao Wan Jian Hu Jiangxi Key Laboratory of Nanobiomaterials Institute of Advanced MaterialsEast China Jiaotong UniversityNanchang 330013China Key Laboratory for Anisotropy and Texture of Materials(ATM) School of Material Science and EngineeringNortheastern UniversityShenyang 110819China

Biomaterial surfaces with satisfied antibacterial activity and appropriate cytocompatibility are a pressing clinical need for orthopedic and dental *** biomaterials have been demonstrated to obtain antibacterial activity and osteogenic property,while the effect of fluorine chemical compositions on antibacterial property and cytocompatibility is rarely *** this end,the coatings with different fluorine chemical compositions on titanium surface were prepared by plasma treatment to verify the antibacterial ability and cytocompatibility of fluorinated *** antibacterial ability was evaluated by using Staphylococcus aureus,and the cell compatibility was investigated with MC3T3-E1 cells in *** results show that both fluorocarbon coating and metal fluorides coating exhibited a hydrophilic and nano-scaled *** than the fluorocarbon coating,the coating composed of metal fluorides presented satisfied bactericide effect and excellent *** antibacterial mechanism is associated with the metal fluorides and released fluoride *** work would provide novel sight in optimizing the surface modification method of fluorinated biomaterials for biomedical applications.

关键词： Titanium Plasma-based technology Fluorine Antibacterial property Cytocompatibility

Antibacterial property and bioadaptability of Ti6Al4V alloy with a silvered gradient nanostructured surface layer

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Rare Metals 2022年第2期41卷 621-629页

作者： Qin Liu Yong-Xiang Li Jing Qiu mian chen Jie Wang Jian Hu Institute of Advanced Materials East China Jiaotong UniversityNanchang 330013China Jiangxi Key Laboratory of Nanobiomaterials East China Jiaotong UniversityNanchang 330013China

In this paper,a silvered gradient nanostructured(GNS)layer was successfully fabricated on Ti6Al4V surface by means of surface ultrasonic rolling treatment(SURT)combined with silvering *** characteristics,mechanical properties,corrosion resistance,antibacterial ability and cytotoxicity of GNS Ag/Ti6Al4V were investigated in comparison with those of coarse-grained(CG)and GNS Ti6Al4V *** to the greatly enhanced diffusion kinetics of Ag in the GNS layer,surface silvering on GNS Ti6Al4V was achieved at a relatively low temperature(500℃),and the release rate of Ag^(+)was substantially accelerated,which endowed GNS Ag/Ti6Al4V with excellent antibacterial ***,improved wear and corrosion resistance of GNS Ag/Ti6Al4V can be achieved without cytotoxicity,indicating excellent bioadaptability.

关键词： Gradient nanostructure Ti6Al4V Silver Antibacterial property Bioadaptability

A continuous and long-term in-situ stress measuring method based on fiber optic. Part I: Theory of inverse differential strain analysis

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Petroleum Science 2024年第2期21卷 1171-1189页

作者： Kun-Peng Zhang mian chen Chang-Jun Zhao Su Wang Yong-Dong Fan State Key Laboratory of Petroleum Resources and Prospecting China University of PetroleumBeijing102249China MOE Key Laboratory of Petroleum Engineering in China University of Petroleum Beijing102249China Beijing International Center for Gas Hydrate School of Earth and Space SciencesPeking UniversityBeijing100871China

A method for in-situ stress measurement via fiber optics was proposed. The method utilizes the relationship between rock mass elastic parameters and in-situ stress. The approach offers the advantage of long-term stress measurements with high spatial resolution and frequency, significantly enhancing the ability to measure in-situ stress. The sensing casing, spirally wrapped with fiber optic, is cemented into the formation to establish a formation sensing nerve. Injecting fluid into the casing generates strain disturbance, establishing the relationship between rock mass properties and treatment ***, an optimization algorithm is established to invert the elastic parameters of formation via fiber optic strains. In the first part of this paper series, we established the theoretical basis for the inverse differential strain analysis method for in-situ stress measurement, which was subsequently verified using an analytical model. This paper is the fundamental basis for the inverse differential strain analysis method.

关键词： In-situ stress Fiber optic Orthotropic elastic Differential evolution ABAQUS